PriA participates in nascent DNA synthesis in Escherichia coli
The question of whether discontinuous DNA replication operates only for the lagging strand or for both strands in E. coli remains unresolved. In this study, the participation of priA, B, C and rep genes in discontinuous DNA replication was examined by analyzing the size distribution of nascent DNA s...
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| Veröffentlicht in: | Molecular biology reports 2010-10, Vol.37 (7), p.3165-3170 |
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| creator | Chen, Yen-Yu Huang, Helen Wang, Tzu-Chien V |
| description | The question of whether discontinuous DNA replication operates only for the lagging strand or for both strands in E. coli remains unresolved. In this study, the participation of priA, B, C and rep genes in discontinuous DNA replication was examined by analyzing the size distribution of nascent DNA synthesized in wild-type, lig-7 and polA4113 genetic backgrounds. Inactivation of priA, but not priB, priC or rep, resulted in a significant increase of high molecular weight (HMW) DNA in the short pulse-labeled DNA in the wild-type lig ⁺ polA ⁺ strains. Inactivation of priA also produced a significant increase of HMW DNA in the nascent DNA synthesized in lig-7 and polA4113 strains. These results indicate that PriA is involved in the discontinuous synthesis of nascent DNA. |
| doi_str_mv | 10.1007/s11033-009-9896-5 |
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In this study, the participation of priA, B, C and rep genes in discontinuous DNA replication was examined by analyzing the size distribution of nascent DNA synthesized in wild-type, lig-7 and polA4113 genetic backgrounds. Inactivation of priA, but not priB, priC or rep, resulted in a significant increase of high molecular weight (HMW) DNA in the short pulse-labeled DNA in the wild-type lig ⁺ polA ⁺ strains. Inactivation of priA also produced a significant increase of HMW DNA in the nascent DNA synthesized in lig-7 and polA4113 strains. These results indicate that PriA is involved in the discontinuous synthesis of nascent DNA.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-009-9896-5</identifier><identifier>PMID: 19823946</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Biomedical and Life Sciences ; Biosynthesis ; Cellular biology ; Deoxyribonucleic acid ; Discontinuous DNA replication ; DNA ; DNA biosynthesis ; DNA Helicases - metabolism ; DNA Replication ; DNA, Bacterial - biosynthesis ; E coli ; Enzymes ; Escherichia coli ; Escherichia coli - enzymology ; Escherichia coli Proteins - metabolism ; Histology ; Life Sciences ; Ligase ; Molecular biology ; Molecular Weight ; Morphology ; Mutation - genetics ; Pol I ; PriA protein ; PriA, B, C ; Rep ; Replication ; Size distribution</subject><ispartof>Molecular biology reports, 2010-10, Vol.37 (7), p.3165-3170</ispartof><rights>Springer Science+Business Media B.V. 2009</rights><rights>Springer Science+Business Media B.V. 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2935-56d8ccaf7397df311a8c6a65eaa74566cd7d6131a327f51333106aa55385b5c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-009-9896-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-009-9896-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27928,27929,41492,42561,51323</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19823946$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Yen-Yu</creatorcontrib><creatorcontrib>Huang, Helen</creatorcontrib><creatorcontrib>Wang, Tzu-Chien V</creatorcontrib><title>PriA participates in nascent DNA synthesis in Escherichia coli</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>The question of whether discontinuous DNA replication operates only for the lagging strand or for both strands in E. coli remains unresolved. In this study, the participation of priA, B, C and rep genes in discontinuous DNA replication was examined by analyzing the size distribution of nascent DNA synthesized in wild-type, lig-7 and polA4113 genetic backgrounds. Inactivation of priA, but not priB, priC or rep, resulted in a significant increase of high molecular weight (HMW) DNA in the short pulse-labeled DNA in the wild-type lig ⁺ polA ⁺ strains. Inactivation of priA also produced a significant increase of HMW DNA in the nascent DNA synthesized in lig-7 and polA4113 strains. These results indicate that PriA is involved in the discontinuous synthesis of nascent DNA.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Cellular biology</subject><subject>Deoxyribonucleic acid</subject><subject>Discontinuous DNA replication</subject><subject>DNA</subject><subject>DNA biosynthesis</subject><subject>DNA Helicases - metabolism</subject><subject>DNA Replication</subject><subject>DNA, Bacterial - biosynthesis</subject><subject>E coli</subject><subject>Enzymes</subject><subject>Escherichia coli</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli Proteins - metabolism</subject><subject>Histology</subject><subject>Life Sciences</subject><subject>Ligase</subject><subject>Molecular biology</subject><subject>Molecular Weight</subject><subject>Morphology</subject><subject>Mutation - genetics</subject><subject>Pol I</subject><subject>PriA protein</subject><subject>PriA, B, C</subject><subject>Rep</subject><subject>Replication</subject><subject>Size distribution</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU1P3DAQhi3UCra0P4ALRFx6SuvJxB77UmlF6YeE2kotZ2twHNZoN1ns7IF_X9OshMSBnnyY533t8SPECcgPICV9zAASsZbS1tZYXasDsQBFWLeWzCuxkCihbo2CI_Em5zspZQukDsURWNOgbfVCfPqV4rLacpqij1ueQq7iUA2cfRim6vOPZZUfhmkVcvw3uMx-FVL0q8iVH9fxrXjd8zqHd_vzWFx_ufxz8a2--vn1-8XyqvaNRVUr3RnvuSe01PUIwMZr1iowU6u09h11GhAYG-oVICJIzawUGnWjvMFj8X7u3abxfhfy5DaxPHG95iGMu-xINw01Euj_pFJAEskW8vwZeTfu0lDWKFDTakPUFghmyKcx5xR6t01xw-nBgXSPEtwswRUJ7lGCUyVzui_e3WxC95TY_3oBmhnIZTTchvR080utZ3Oo59HxbYrZXf8uKxfHxlhDBv8C4dGYKQ</recordid><startdate>201010</startdate><enddate>201010</enddate><creator>Chen, Yen-Yu</creator><creator>Huang, Helen</creator><creator>Wang, Tzu-Chien V</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>7QL</scope><scope>C1K</scope></search><sort><creationdate>201010</creationdate><title>PriA participates in nascent DNA synthesis in Escherichia coli</title><author>Chen, Yen-Yu ; Huang, Helen ; Wang, Tzu-Chien V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2935-56d8ccaf7397df311a8c6a65eaa74566cd7d6131a327f51333106aa55385b5c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Cellular biology</topic><topic>Deoxyribonucleic acid</topic><topic>Discontinuous DNA replication</topic><topic>DNA</topic><topic>DNA biosynthesis</topic><topic>DNA Helicases - metabolism</topic><topic>DNA Replication</topic><topic>DNA, Bacterial - biosynthesis</topic><topic>E coli</topic><topic>Enzymes</topic><topic>Escherichia coli</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli Proteins - metabolism</topic><topic>Histology</topic><topic>Life Sciences</topic><topic>Ligase</topic><topic>Molecular biology</topic><topic>Molecular Weight</topic><topic>Morphology</topic><topic>Mutation - genetics</topic><topic>Pol I</topic><topic>PriA protein</topic><topic>PriA, B, C</topic><topic>Rep</topic><topic>Replication</topic><topic>Size distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Yen-Yu</creatorcontrib><creatorcontrib>Huang, Helen</creatorcontrib><creatorcontrib>Wang, Tzu-Chien V</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Yen-Yu</au><au>Huang, Helen</au><au>Wang, Tzu-Chien V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PriA participates in nascent DNA synthesis in Escherichia coli</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2010-10</date><risdate>2010</risdate><volume>37</volume><issue>7</issue><spage>3165</spage><epage>3170</epage><pages>3165-3170</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>The question of whether discontinuous DNA replication operates only for the lagging strand or for both strands in E. coli remains unresolved. In this study, the participation of priA, B, C and rep genes in discontinuous DNA replication was examined by analyzing the size distribution of nascent DNA synthesized in wild-type, lig-7 and polA4113 genetic backgrounds. Inactivation of priA, but not priB, priC or rep, resulted in a significant increase of high molecular weight (HMW) DNA in the short pulse-labeled DNA in the wild-type lig ⁺ polA ⁺ strains. Inactivation of priA also produced a significant increase of HMW DNA in the nascent DNA synthesized in lig-7 and polA4113 strains. These results indicate that PriA is involved in the discontinuous synthesis of nascent DNA.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><pmid>19823946</pmid><doi>10.1007/s11033-009-9896-5</doi><tpages>6</tpages></addata></record> |
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| subjects | Animal Anatomy Animal Biochemistry Biomedical and Life Sciences Biosynthesis Cellular biology Deoxyribonucleic acid Discontinuous DNA replication DNA DNA biosynthesis DNA Helicases - metabolism DNA Replication DNA, Bacterial - biosynthesis E coli Enzymes Escherichia coli Escherichia coli - enzymology Escherichia coli Proteins - metabolism Histology Life Sciences Ligase Molecular biology Molecular Weight Morphology Mutation - genetics Pol I PriA protein PriA, B, C Rep Replication Size distribution |
| title | PriA participates in nascent DNA synthesis in Escherichia coli |
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