Laparoscopic surgery and Kupffer cell activation
Evidence tends to support a relative preservation of the systemic immune response with laparoscopy as compared with laparotomy. However, the role of hepatic macrophages, or Kupffer cells, in modulating this immune advantage is unknown. This study investigated the functions of Kupffer cells after eit...
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| Veröffentlicht in: | Surgical endoscopy 2000-12, Vol.14 (12), p.1171-1176 |
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| creator | VITTIMBERGA, F. J NOLAN, B PERUGINI, R. A SPECTOR, L CALLERY, M. P |
| description | Evidence tends to support a relative preservation of the systemic immune response with laparoscopy as compared with laparotomy. However, the role of hepatic macrophages, or Kupffer cells, in modulating this immune advantage is unknown. This study investigated the functions of Kupffer cells after either laparoscopy or laparotomy in a rat model.
Rats underwent laparoscopy, laparotomy, or control operations. Kupffer cells were harvested, cultured, and stimulated with lipopolysaccharide. Culture supernatants were analyzed for tumor necrosis factor (TNF-alpha) and interleukin-6 (IL-6). Cytoplasmic lysates were analyzed for activation of two mitogen-activated protein kinases (MAPKs).
Production of TNF-alpha and IL-6 was similar in laparoscopy, laparotomy, and control groups. Both laparotomy and laparoscopy showed increased activation of p38 MAPK as compared with controls. Activation of ERK1/2 was decreased during laparotomy as compared with laparoscopy.
Although cytokine production was similar in the laparoscopy and laparotomy groups, changes in MAPK activation suggest that intracellular pathways are more affected during laparotomy than during laparoscopy. |
| doi_str_mv | 10.1007/s004640010065 |
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Rats underwent laparoscopy, laparotomy, or control operations. Kupffer cells were harvested, cultured, and stimulated with lipopolysaccharide. Culture supernatants were analyzed for tumor necrosis factor (TNF-alpha) and interleukin-6 (IL-6). Cytoplasmic lysates were analyzed for activation of two mitogen-activated protein kinases (MAPKs).
Production of TNF-alpha and IL-6 was similar in laparoscopy, laparotomy, and control groups. Both laparotomy and laparoscopy showed increased activation of p38 MAPK as compared with controls. Activation of ERK1/2 was decreased during laparotomy as compared with laparoscopy.
Although cytokine production was similar in the laparoscopy and laparotomy groups, changes in MAPK activation suggest that intracellular pathways are more affected during laparotomy than during laparoscopy.</description><identifier>ISSN: 0930-2794</identifier><identifier>EISSN: 1432-2218</identifier><identifier>DOI: 10.1007/s004640010065</identifier><identifier>PMID: 11148792</identifier><identifier>CODEN: SUREEX</identifier><language>eng</language><publisher>New York, NY: Springer</publisher><subject>Abdomen ; Animals ; Biological and medical sciences ; Blotting, Western - methods ; Blotting, Western - statistics & numerical data ; Cell Separation - methods ; Enzyme Activation - drug effects ; Enzyme-Linked Immunosorbent Assay - methods ; Enzyme-Linked Immunosorbent Assay - statistics & numerical data ; Interleukin-6 - analysis ; Interleukin-6 - biosynthesis ; Kupffer Cells - drug effects ; Kupffer Cells - metabolism ; Laparoscopy ; Laparotomy ; Lipopolysaccharides - pharmacology ; Macrophage Activation - drug effects ; Medical sciences ; Mitogen-Activated Protein Kinases - analysis ; Mitogen-Activated Protein Kinases - drug effects ; Mitogen-Activated Protein Kinases - metabolism ; Pneumoperitoneum, Artificial ; Rats ; Rats, Sprague-Dawley ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Surgery of the digestive system ; Tumor Necrosis Factor-alpha - analysis ; Tumor Necrosis Factor-alpha - biosynthesis</subject><ispartof>Surgical endoscopy, 2000-12, Vol.14 (12), p.1171-1176</ispartof><rights>2001 INIST-CNRS</rights><rights>Springer-Verlag New York Inc. 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c376t-44cffabbcd8d5af9ca8b7fa38f4e5bdd2441e98f5d15f377f9682dc7681c628b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>310,311,315,782,786,791,792,23937,23938,25147,27931,27932</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=870003$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11148792$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>VITTIMBERGA, F. J</creatorcontrib><creatorcontrib>NOLAN, B</creatorcontrib><creatorcontrib>PERUGINI, R. A</creatorcontrib><creatorcontrib>SPECTOR, L</creatorcontrib><creatorcontrib>CALLERY, M. P</creatorcontrib><title>Laparoscopic surgery and Kupffer cell activation</title><title>Surgical endoscopy</title><addtitle>Surg Endosc</addtitle><description>Evidence tends to support a relative preservation of the systemic immune response with laparoscopy as compared with laparotomy. However, the role of hepatic macrophages, or Kupffer cells, in modulating this immune advantage is unknown. This study investigated the functions of Kupffer cells after either laparoscopy or laparotomy in a rat model.
Rats underwent laparoscopy, laparotomy, or control operations. Kupffer cells were harvested, cultured, and stimulated with lipopolysaccharide. Culture supernatants were analyzed for tumor necrosis factor (TNF-alpha) and interleukin-6 (IL-6). Cytoplasmic lysates were analyzed for activation of two mitogen-activated protein kinases (MAPKs).
Production of TNF-alpha and IL-6 was similar in laparoscopy, laparotomy, and control groups. Both laparotomy and laparoscopy showed increased activation of p38 MAPK as compared with controls. Activation of ERK1/2 was decreased during laparotomy as compared with laparoscopy.
Although cytokine production was similar in the laparoscopy and laparotomy groups, changes in MAPK activation suggest that intracellular pathways are more affected during laparotomy than during laparoscopy.</description><subject>Abdomen</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western - methods</subject><subject>Blotting, Western - statistics & numerical data</subject><subject>Cell Separation - methods</subject><subject>Enzyme Activation - drug effects</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzyme-Linked Immunosorbent Assay - statistics & numerical data</subject><subject>Interleukin-6 - analysis</subject><subject>Interleukin-6 - biosynthesis</subject><subject>Kupffer Cells - drug effects</subject><subject>Kupffer Cells - metabolism</subject><subject>Laparoscopy</subject><subject>Laparotomy</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Macrophage Activation - drug effects</subject><subject>Medical sciences</subject><subject>Mitogen-Activated Protein Kinases - analysis</subject><subject>Mitogen-Activated Protein Kinases - drug effects</subject><subject>Mitogen-Activated Protein Kinases - metabolism</subject><subject>Pneumoperitoneum, Artificial</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Surgery (general aspects). 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P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c376t-44cffabbcd8d5af9ca8b7fa38f4e5bdd2441e98f5d15f377f9682dc7681c628b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Abdomen</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western - methods</topic><topic>Blotting, Western - statistics & numerical data</topic><topic>Cell Separation - methods</topic><topic>Enzyme Activation - drug effects</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzyme-Linked Immunosorbent Assay - statistics & numerical data</topic><topic>Interleukin-6 - analysis</topic><topic>Interleukin-6 - biosynthesis</topic><topic>Kupffer Cells - drug effects</topic><topic>Kupffer Cells - metabolism</topic><topic>Laparoscopy</topic><topic>Laparotomy</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Macrophage Activation - drug effects</topic><topic>Medical sciences</topic><topic>Mitogen-Activated Protein Kinases - analysis</topic><topic>Mitogen-Activated Protein Kinases - drug effects</topic><topic>Mitogen-Activated Protein Kinases - metabolism</topic><topic>Pneumoperitoneum, Artificial</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Surgery (general aspects). 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Rats underwent laparoscopy, laparotomy, or control operations. Kupffer cells were harvested, cultured, and stimulated with lipopolysaccharide. Culture supernatants were analyzed for tumor necrosis factor (TNF-alpha) and interleukin-6 (IL-6). Cytoplasmic lysates were analyzed for activation of two mitogen-activated protein kinases (MAPKs).
Production of TNF-alpha and IL-6 was similar in laparoscopy, laparotomy, and control groups. Both laparotomy and laparoscopy showed increased activation of p38 MAPK as compared with controls. Activation of ERK1/2 was decreased during laparotomy as compared with laparoscopy.
Although cytokine production was similar in the laparoscopy and laparotomy groups, changes in MAPK activation suggest that intracellular pathways are more affected during laparotomy than during laparoscopy.</abstract><cop>New York, NY</cop><pub>Springer</pub><pmid>11148792</pmid><doi>10.1007/s004640010065</doi><tpages>6</tpages></addata></record> |
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| subjects | Abdomen Animals Biological and medical sciences Blotting, Western - methods Blotting, Western - statistics & numerical data Cell Separation - methods Enzyme Activation - drug effects Enzyme-Linked Immunosorbent Assay - methods Enzyme-Linked Immunosorbent Assay - statistics & numerical data Interleukin-6 - analysis Interleukin-6 - biosynthesis Kupffer Cells - drug effects Kupffer Cells - metabolism Laparoscopy Laparotomy Lipopolysaccharides - pharmacology Macrophage Activation - drug effects Medical sciences Mitogen-Activated Protein Kinases - analysis Mitogen-Activated Protein Kinases - drug effects Mitogen-Activated Protein Kinases - metabolism Pneumoperitoneum, Artificial Rats Rats, Sprague-Dawley Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Surgery of the digestive system Tumor Necrosis Factor-alpha - analysis Tumor Necrosis Factor-alpha - biosynthesis |
| title | Laparoscopic surgery and Kupffer cell activation |
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