Levels of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, inflammatory cytokines and species-specific immunoglobulin G in generalized aggressive and chronic periodontitis
Casarin RCV, Del Peloso Ribeiro É, Mariano FS, Nociti FH Jr, Casati MZ, Gonçalves RB. Levels of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, inflammatory cytokines and species‐specific immunoglobulin G in generalized aggressive and chronic periodontitis. J Periodont Res 2010; 45:...
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| description | Casarin RCV, Del Peloso Ribeiro É, Mariano FS, Nociti FH Jr, Casati MZ, Gonçalves RB. Levels of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, inflammatory cytokines and species‐specific immunoglobulin G in generalized aggressive and chronic periodontitis. J Periodont Res 2010; 45: 635–642. © 2010 John Wiley & Sons A/S
Background and Objective: Aggressive periodontitis pathogenesis still is not completely understood in the literature regarding the relationship between microbial and inflammatory aspects. So this study aimed to compare microbial and inflammatory patterns in the gingival crevicular fluid of generalized aggressive and chronic periodontitis patients.
Material and Methods: Forty aggressive and 28 chronic periodontitis patients were selected. Biofilm and gingival crevicular fluid were collected from a deep pocket (periodontal probing depth >7 mm) and a moderate pocket (periodontal probing depth = 5 mm) of each patient, and microbiological and immunoenzymatic assays were performed. Real‐time PCR was used to determine quantities of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Enzyme‐linked immunosorbent assay (ELISA) was employed to determine gingival crevicular fluid levels of interleukin‐1β, interferon‐γ, prostaglandin E2 and interleukin‐10. In addition, immunoglobulin G (IgG) levels against A. actinomycetemcomitans and P. gingivalis lipopolysaccharide were also determined by ELISA. Analysis of variance/Tukey test, Mann–Whitney U‐test and the Pearson correlation test were used to determine differences and correlations between variables analysed (α = 5%).
Results: Patients suffering from generalized aggressive periodontitis had their mouth colonized by higher amounts of A. actinomycetemcomitans and P. gingivalis than chronic periodontitis patients. Conversely, the gingival crevicular fluid levels of IgG against both pathogens were statistically inferior in aggressive periodontitis patients (p |
| doi_str_mv | 10.1111/j.1600-0765.2010.01278.x |
| format | Article |
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Background and Objective: Aggressive periodontitis pathogenesis still is not completely understood in the literature regarding the relationship between microbial and inflammatory aspects. So this study aimed to compare microbial and inflammatory patterns in the gingival crevicular fluid of generalized aggressive and chronic periodontitis patients.
Material and Methods: Forty aggressive and 28 chronic periodontitis patients were selected. Biofilm and gingival crevicular fluid were collected from a deep pocket (periodontal probing depth >7 mm) and a moderate pocket (periodontal probing depth = 5 mm) of each patient, and microbiological and immunoenzymatic assays were performed. Real‐time PCR was used to determine quantities of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Enzyme‐linked immunosorbent assay (ELISA) was employed to determine gingival crevicular fluid levels of interleukin‐1β, interferon‐γ, prostaglandin E2 and interleukin‐10. In addition, immunoglobulin G (IgG) levels against A. actinomycetemcomitans and P. gingivalis lipopolysaccharide were also determined by ELISA. Analysis of variance/Tukey test, Mann–Whitney U‐test and the Pearson correlation test were used to determine differences and correlations between variables analysed (α = 5%).
Results: Patients suffering from generalized aggressive periodontitis had their mouth colonized by higher amounts of A. actinomycetemcomitans and P. gingivalis than chronic periodontitis patients. Conversely, the gingival crevicular fluid levels of IgG against both pathogens were statistically inferior in aggressive periodontitis patients (p < 0.05). With regard to gingival crevicular fluid levels of cytokines, aggressive periodontitis patients presented reduced levels of interleukin‐10 (p < 0.05).
Conclusion: In comparison to chronic periodontitis, generalized aggressive periodontitis patients have an imbalance in the host response, with reduced levels of interleukin‐10 and IgG, and increased periodontal pathogens.</description><identifier>ISSN: 0022-3484</identifier><identifier>EISSN: 1600-0765</identifier><identifier>DOI: 10.1111/j.1600-0765.2010.01278.x</identifier><identifier>PMID: 20546109</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Aggregatibacter actinomycetemcomitans - isolation & purification ; aggressive periodontitis ; Aggressive Periodontitis - immunology ; Aggressive Periodontitis - microbiology ; Antibodies, Bacterial - analysis ; bacteria ; Biofilms ; Biological and medical sciences ; Chi-Square Distribution ; chronic periodontitis ; Chronic Periodontitis - immunology ; Chronic Periodontitis - microbiology ; cytokine ; Cytokines ; Cytokines - analysis ; Cytokines - metabolism ; Dentistry ; Dinoprostone - analysis ; Dinoprostone - metabolism ; DNA, Bacterial - analysis ; Enzyme-linked immunosorbent assay ; Facial bones, jaws, teeth, parodontium: diseases, semeiology ; Female ; g-Interferon ; Gingiva ; Gingival Crevicular Fluid - chemistry ; Gingival Crevicular Fluid - immunology ; Gingival Crevicular Fluid - microbiology ; Host-Pathogen Interactions ; Humans ; immunoglobulin ; Immunoglobulin G ; Immunoglobulin G - analysis ; Inflammation ; Interferon-gamma - analysis ; Interleukin 10 ; Interleukins - analysis ; Interleukins - metabolism ; Lipopolysaccharides - immunology ; Male ; Medical sciences ; Middle Aged ; Non tumoral diseases ; Normal Distribution ; Otorhinolaryngology. Stomatology ; pathogenesis ; Pathogens ; Periodontal Pocket - immunology ; Periodontal Pocket - microbiology ; Periodontitis ; Porphyromonas gingivalis ; Porphyromonas gingivalis - isolation & purification ; Species Specificity ; Statistics, Nonparametric</subject><ispartof>Journal of periodontal research, 2010-10, Vol.45 (5), p.635-642</ispartof><rights>2010 John Wiley & Sons A/S</rights><rights>2015 INIST-CNRS</rights><rights>(c) 2010 John Wiley & Sons A/S.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4858-a4a5e6236e0885f757c932ddf6f0aa03029176777eb7783f8f726aed3c03d3213</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0765.2010.01278.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0765.2010.01278.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23217126$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20546109$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Casarin, R. C. V.</creatorcontrib><creatorcontrib>Del Peloso Ribeiro, É.</creatorcontrib><creatorcontrib>Mariano, F. S.</creatorcontrib><creatorcontrib>Nociti Jr, F. H.</creatorcontrib><creatorcontrib>Casati, M. Z.</creatorcontrib><creatorcontrib>Gonçalves, R. B.</creatorcontrib><title>Levels of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, inflammatory cytokines and species-specific immunoglobulin G in generalized aggressive and chronic periodontitis</title><title>Journal of periodontal research</title><addtitle>J Periodontal Res</addtitle><description>Casarin RCV, Del Peloso Ribeiro É, Mariano FS, Nociti FH Jr, Casati MZ, Gonçalves RB. Levels of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, inflammatory cytokines and species‐specific immunoglobulin G in generalized aggressive and chronic periodontitis. J Periodont Res 2010; 45: 635–642. © 2010 John Wiley & Sons A/S
Background and Objective: Aggressive periodontitis pathogenesis still is not completely understood in the literature regarding the relationship between microbial and inflammatory aspects. So this study aimed to compare microbial and inflammatory patterns in the gingival crevicular fluid of generalized aggressive and chronic periodontitis patients.
Material and Methods: Forty aggressive and 28 chronic periodontitis patients were selected. Biofilm and gingival crevicular fluid were collected from a deep pocket (periodontal probing depth >7 mm) and a moderate pocket (periodontal probing depth = 5 mm) of each patient, and microbiological and immunoenzymatic assays were performed. Real‐time PCR was used to determine quantities of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Enzyme‐linked immunosorbent assay (ELISA) was employed to determine gingival crevicular fluid levels of interleukin‐1β, interferon‐γ, prostaglandin E2 and interleukin‐10. In addition, immunoglobulin G (IgG) levels against A. actinomycetemcomitans and P. gingivalis lipopolysaccharide were also determined by ELISA. Analysis of variance/Tukey test, Mann–Whitney U‐test and the Pearson correlation test were used to determine differences and correlations between variables analysed (α = 5%).
Results: Patients suffering from generalized aggressive periodontitis had their mouth colonized by higher amounts of A. actinomycetemcomitans and P. gingivalis than chronic periodontitis patients. Conversely, the gingival crevicular fluid levels of IgG against both pathogens were statistically inferior in aggressive periodontitis patients (p < 0.05). With regard to gingival crevicular fluid levels of cytokines, aggressive periodontitis patients presented reduced levels of interleukin‐10 (p < 0.05).
Conclusion: In comparison to chronic periodontitis, generalized aggressive periodontitis patients have an imbalance in the host response, with reduced levels of interleukin‐10 and IgG, and increased periodontal pathogens.</description><subject>Adult</subject><subject>Aggregatibacter actinomycetemcomitans - isolation & purification</subject><subject>aggressive periodontitis</subject><subject>Aggressive Periodontitis - immunology</subject><subject>Aggressive Periodontitis - microbiology</subject><subject>Antibodies, Bacterial - analysis</subject><subject>bacteria</subject><subject>Biofilms</subject><subject>Biological and medical sciences</subject><subject>Chi-Square Distribution</subject><subject>chronic periodontitis</subject><subject>Chronic Periodontitis - immunology</subject><subject>Chronic Periodontitis - microbiology</subject><subject>cytokine</subject><subject>Cytokines</subject><subject>Cytokines - analysis</subject><subject>Cytokines - metabolism</subject><subject>Dentistry</subject><subject>Dinoprostone - analysis</subject><subject>Dinoprostone - metabolism</subject><subject>DNA, Bacterial - analysis</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Facial bones, jaws, teeth, parodontium: diseases, semeiology</subject><subject>Female</subject><subject>g-Interferon</subject><subject>Gingiva</subject><subject>Gingival Crevicular Fluid - chemistry</subject><subject>Gingival Crevicular Fluid - immunology</subject><subject>Gingival Crevicular Fluid - microbiology</subject><subject>Host-Pathogen Interactions</subject><subject>Humans</subject><subject>immunoglobulin</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin G - analysis</subject><subject>Inflammation</subject><subject>Interferon-gamma - analysis</subject><subject>Interleukin 10</subject><subject>Interleukins - analysis</subject><subject>Interleukins - metabolism</subject><subject>Lipopolysaccharides - immunology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Non tumoral diseases</subject><subject>Normal Distribution</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>pathogenesis</subject><subject>Pathogens</subject><subject>Periodontal Pocket - immunology</subject><subject>Periodontal Pocket - microbiology</subject><subject>Periodontitis</subject><subject>Porphyromonas gingivalis</subject><subject>Porphyromonas gingivalis - isolation & purification</subject><subject>Species Specificity</subject><subject>Statistics, Nonparametric</subject><issn>0022-3484</issn><issn>1600-0765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFksGO0zAURSMEYsrALyBvEBtSHDuxkwWL0WgooFLQaBBLy3VeMu7EdrGT0vBr_BxOW8oSL_xs33OfnuSbJCjD8yyut5t5xjBOMWfFnOD4ijPCy_n-UTI7C4-TGcaEpDQv84vkWQgbHO-MV0-TC4KLnGW4miW_l7CDLiDXoKu29dDKXq-l6sGjuGvrzKigB6Oc0b204Q366vz2fvTOOCsDarVt9U52OiraNp00RvbOj0iNvXvQFgKStkZhC0pDSA-10QppYwbr2s6th05btIhm1IIFH1v9ghrJaZgQ9A4OfnXvnY22LXjtamd73evwPHnSyC7Ai1O9TL69v7m7_pAuvyw-Xl8tU5WXRZnKXBbACGWAy7JoeMFVRUldN6zBUmKKSZVxxjmHNeclbcqGEyahpgrTmpKMXiavj3233v0YIPTC6KCg66QFNwTBGSEcc0z-T-ZlxYuqKCP58kQOawO12HptpB_F35-JwKsTIIOSXeOlVTr84-JgPCMscu-O3E_dwXjWMyympIiNmAIhpkCIKSnikBSxF59ub6ZT9KdHvw497M9-6R8E45QX4vtqIW5XK7zid58Fpn8AhFDFeA</recordid><startdate>201010</startdate><enddate>201010</enddate><creator>Casarin, R. C. V.</creator><creator>Del Peloso Ribeiro, É.</creator><creator>Mariano, F. S.</creator><creator>Nociti Jr, F. H.</creator><creator>Casati, M. Z.</creator><creator>Gonçalves, R. B.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope></search><sort><creationdate>201010</creationdate><title>Levels of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, inflammatory cytokines and species-specific immunoglobulin G in generalized aggressive and chronic periodontitis</title><author>Casarin, R. C. V. ; Del Peloso Ribeiro, É. ; Mariano, F. S. ; Nociti Jr, F. H. ; Casati, M. Z. ; Gonçalves, R. B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4858-a4a5e6236e0885f757c932ddf6f0aa03029176777eb7783f8f726aed3c03d3213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adult</topic><topic>Aggregatibacter actinomycetemcomitans - isolation & purification</topic><topic>aggressive periodontitis</topic><topic>Aggressive Periodontitis - immunology</topic><topic>Aggressive Periodontitis - microbiology</topic><topic>Antibodies, Bacterial - analysis</topic><topic>bacteria</topic><topic>Biofilms</topic><topic>Biological and medical sciences</topic><topic>Chi-Square Distribution</topic><topic>chronic periodontitis</topic><topic>Chronic Periodontitis - immunology</topic><topic>Chronic Periodontitis - microbiology</topic><topic>cytokine</topic><topic>Cytokines</topic><topic>Cytokines - analysis</topic><topic>Cytokines - metabolism</topic><topic>Dentistry</topic><topic>Dinoprostone - analysis</topic><topic>Dinoprostone - metabolism</topic><topic>DNA, Bacterial - analysis</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Facial bones, jaws, teeth, parodontium: diseases, semeiology</topic><topic>Female</topic><topic>g-Interferon</topic><topic>Gingiva</topic><topic>Gingival Crevicular Fluid - chemistry</topic><topic>Gingival Crevicular Fluid - immunology</topic><topic>Gingival Crevicular Fluid - microbiology</topic><topic>Host-Pathogen Interactions</topic><topic>Humans</topic><topic>immunoglobulin</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin G - analysis</topic><topic>Inflammation</topic><topic>Interferon-gamma - analysis</topic><topic>Interleukin 10</topic><topic>Interleukins - analysis</topic><topic>Interleukins - metabolism</topic><topic>Lipopolysaccharides - immunology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Non tumoral diseases</topic><topic>Normal Distribution</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>pathogenesis</topic><topic>Pathogens</topic><topic>Periodontal Pocket - immunology</topic><topic>Periodontal Pocket - microbiology</topic><topic>Periodontitis</topic><topic>Porphyromonas gingivalis</topic><topic>Porphyromonas gingivalis - isolation & purification</topic><topic>Species Specificity</topic><topic>Statistics, Nonparametric</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Casarin, R. C. V.</creatorcontrib><creatorcontrib>Del Peloso Ribeiro, É.</creatorcontrib><creatorcontrib>Mariano, F. S.</creatorcontrib><creatorcontrib>Nociti Jr, F. H.</creatorcontrib><creatorcontrib>Casati, M. Z.</creatorcontrib><creatorcontrib>Gonçalves, R. B.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of periodontal research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Casarin, R. C. V.</au><au>Del Peloso Ribeiro, É.</au><au>Mariano, F. S.</au><au>Nociti Jr, F. H.</au><au>Casati, M. Z.</au><au>Gonçalves, R. B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Levels of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, inflammatory cytokines and species-specific immunoglobulin G in generalized aggressive and chronic periodontitis</atitle><jtitle>Journal of periodontal research</jtitle><addtitle>J Periodontal Res</addtitle><date>2010-10</date><risdate>2010</risdate><volume>45</volume><issue>5</issue><spage>635</spage><epage>642</epage><pages>635-642</pages><issn>0022-3484</issn><eissn>1600-0765</eissn><abstract>Casarin RCV, Del Peloso Ribeiro É, Mariano FS, Nociti FH Jr, Casati MZ, Gonçalves RB. Levels of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, inflammatory cytokines and species‐specific immunoglobulin G in generalized aggressive and chronic periodontitis. J Periodont Res 2010; 45: 635–642. © 2010 John Wiley & Sons A/S
Background and Objective: Aggressive periodontitis pathogenesis still is not completely understood in the literature regarding the relationship between microbial and inflammatory aspects. So this study aimed to compare microbial and inflammatory patterns in the gingival crevicular fluid of generalized aggressive and chronic periodontitis patients.
Material and Methods: Forty aggressive and 28 chronic periodontitis patients were selected. Biofilm and gingival crevicular fluid were collected from a deep pocket (periodontal probing depth >7 mm) and a moderate pocket (periodontal probing depth = 5 mm) of each patient, and microbiological and immunoenzymatic assays were performed. Real‐time PCR was used to determine quantities of Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis. Enzyme‐linked immunosorbent assay (ELISA) was employed to determine gingival crevicular fluid levels of interleukin‐1β, interferon‐γ, prostaglandin E2 and interleukin‐10. In addition, immunoglobulin G (IgG) levels against A. actinomycetemcomitans and P. gingivalis lipopolysaccharide were also determined by ELISA. Analysis of variance/Tukey test, Mann–Whitney U‐test and the Pearson correlation test were used to determine differences and correlations between variables analysed (α = 5%).
Results: Patients suffering from generalized aggressive periodontitis had their mouth colonized by higher amounts of A. actinomycetemcomitans and P. gingivalis than chronic periodontitis patients. Conversely, the gingival crevicular fluid levels of IgG against both pathogens were statistically inferior in aggressive periodontitis patients (p < 0.05). With regard to gingival crevicular fluid levels of cytokines, aggressive periodontitis patients presented reduced levels of interleukin‐10 (p < 0.05).
Conclusion: In comparison to chronic periodontitis, generalized aggressive periodontitis patients have an imbalance in the host response, with reduced levels of interleukin‐10 and IgG, and increased periodontal pathogens.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20546109</pmid><doi>10.1111/j.1600-0765.2010.01278.x</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of periodontal research, 2010-10, Vol.45 (5), p.635-642 |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Adult Aggregatibacter actinomycetemcomitans - isolation & purification aggressive periodontitis Aggressive Periodontitis - immunology Aggressive Periodontitis - microbiology Antibodies, Bacterial - analysis bacteria Biofilms Biological and medical sciences Chi-Square Distribution chronic periodontitis Chronic Periodontitis - immunology Chronic Periodontitis - microbiology cytokine Cytokines Cytokines - analysis Cytokines - metabolism Dentistry Dinoprostone - analysis Dinoprostone - metabolism DNA, Bacterial - analysis Enzyme-linked immunosorbent assay Facial bones, jaws, teeth, parodontium: diseases, semeiology Female g-Interferon Gingiva Gingival Crevicular Fluid - chemistry Gingival Crevicular Fluid - immunology Gingival Crevicular Fluid - microbiology Host-Pathogen Interactions Humans immunoglobulin Immunoglobulin G Immunoglobulin G - analysis Inflammation Interferon-gamma - analysis Interleukin 10 Interleukins - analysis Interleukins - metabolism Lipopolysaccharides - immunology Male Medical sciences Middle Aged Non tumoral diseases Normal Distribution Otorhinolaryngology. Stomatology pathogenesis Pathogens Periodontal Pocket - immunology Periodontal Pocket - microbiology Periodontitis Porphyromonas gingivalis Porphyromonas gingivalis - isolation & purification Species Specificity Statistics, Nonparametric |
| title | Levels of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, inflammatory cytokines and species-specific immunoglobulin G in generalized aggressive and chronic periodontitis |
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