Synthetic DNA delivery systems
The ability to safely and efficiently transfer foreign DNA into cells is a fundamental goal in biotechnology. Toward this end, rapid advances have recently been made in our understanding of mechanisms for DNA stability and transport within cells. Current synthetic DNA delivery systems are versatile...
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| Veröffentlicht in: | Nature biotechnology 2000-01, Vol.18 (1), p.33-37 |
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| creator | Saltzman, W. Mark Luo, Dan |
| description | The ability to safely and efficiently transfer foreign DNA into cells is a fundamental goal in biotechnology. Toward this end, rapid advances have recently been made in our understanding of mechanisms for DNA stability and transport within cells. Current synthetic DNA delivery systems are versatile and safe, but substantially less efficient than viruses. Indeed, most current systems address only one of the obstacles to DNA delivery by enhancing DNA uptake. In fact, the effectiveness of gene expression is also dependent on several additional factors, including the release of intracellular DNA, stability of DNA in the cytoplasm, unpackaging of the DNA-vector complex, and the targeting of DNA to the nucleus. Delivery systems of the future must fully accommodate all these processes to effectively shepherd DNA across the plasma membrane, through the hostile intracellular environment, and into the nucleus. |
| doi_str_mv | 10.1038/71889 |
| format | Article |
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Mark ; Luo, Dan</creator><creatorcontrib>Saltzman, W. Mark ; Luo, Dan</creatorcontrib><description>The ability to safely and efficiently transfer foreign DNA into cells is a fundamental goal in biotechnology. Toward this end, rapid advances have recently been made in our understanding of mechanisms for DNA stability and transport within cells. Current synthetic DNA delivery systems are versatile and safe, but substantially less efficient than viruses. Indeed, most current systems address only one of the obstacles to DNA delivery by enhancing DNA uptake. In fact, the effectiveness of gene expression is also dependent on several additional factors, including the release of intracellular DNA, stability of DNA in the cytoplasm, unpackaging of the DNA-vector complex, and the targeting of DNA to the nucleus. Delivery systems of the future must fully accommodate all these processes to effectively shepherd DNA across the plasma membrane, through the hostile intracellular environment, and into the nucleus.</description><identifier>ISSN: 1087-0156</identifier><identifier>EISSN: 1546-1696</identifier><identifier>DOI: 10.1038/71889</identifier><identifier>PMID: 10625387</identifier><identifier>CODEN: NABIF9</identifier><language>eng</language><publisher>New York, NY: Nature</publisher><subject>Animals ; Biological and medical sciences ; Biological Transport ; Biotechnology ; Cell division ; Cell Nucleus - metabolism ; Deoxyribonucleic acid ; DNA ; DNA - administration & dosage ; DNA - metabolism ; Drug Delivery Systems - methods ; Drug Delivery Systems - trends ; Efficiency ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene therapy ; Health. Pharmaceutical industry ; Humans ; Industrial applications and implications. 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Mark</creatorcontrib><creatorcontrib>Luo, Dan</creatorcontrib><title>Synthetic DNA delivery systems</title><title>Nature biotechnology</title><addtitle>Nat Biotechnol</addtitle><description>The ability to safely and efficiently transfer foreign DNA into cells is a fundamental goal in biotechnology. Toward this end, rapid advances have recently been made in our understanding of mechanisms for DNA stability and transport within cells. Current synthetic DNA delivery systems are versatile and safe, but substantially less efficient than viruses. Indeed, most current systems address only one of the obstacles to DNA delivery by enhancing DNA uptake. In fact, the effectiveness of gene expression is also dependent on several additional factors, including the release of intracellular DNA, stability of DNA in the cytoplasm, unpackaging of the DNA-vector complex, and the targeting of DNA to the nucleus. Delivery systems of the future must fully accommodate all these processes to effectively shepherd DNA across the plasma membrane, through the hostile intracellular environment, and into the nucleus.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Biotechnology</subject><subject>Cell division</subject><subject>Cell Nucleus - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - administration & dosage</subject><subject>DNA - metabolism</subject><subject>Drug Delivery Systems - methods</subject><subject>Drug Delivery Systems - trends</subject><subject>Efficiency</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene therapy</subject><subject>Health. Pharmaceutical industry</subject><subject>Humans</subject><subject>Industrial applications and implications. 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| ispartof | Nature biotechnology, 2000-01, Vol.18 (1), p.33-37 |
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| source | MEDLINE; Nature; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Biological Transport Biotechnology Cell division Cell Nucleus - metabolism Deoxyribonucleic acid DNA DNA - administration & dosage DNA - metabolism Drug Delivery Systems - methods Drug Delivery Systems - trends Efficiency Fundamental and applied biological sciences. Psychology Gene expression Gene therapy Health. Pharmaceutical industry Humans Industrial applications and implications. Economical aspects Laboratories Nuclear Localization Signals Plasma Toxicity Transfection - methods |
| title | Synthetic DNA delivery systems |
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