Transcripts of the MHM region on the chicken Z chromosome accumulate as non-coding RNA in the nucleus of female cells adjacent to the DMRT1 locus
The male hypermethylated (MHM) region, located near the middle of the short arm of the Z chromosome of chickens, consists of approximately 210 tandem repeats of a BamHI 2.2-kb sequence unit. Cytosines of the CpG dinucleotides of this region are extensively methylated on the two Z chromosomes in the...
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| Veröffentlicht in: | Chromosome research 2001-01, Vol.9 (2), p.147-165 |
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| creator | Teranishi, M Shimada, Y Hori, T Nakabayashi, O Kikuchi, T Macleod, T Pym, R Sheldon, B Solovei, I Macgregor, H Mizuno, S |
| description | The male hypermethylated (MHM) region, located near the middle of the short arm of the Z chromosome of chickens, consists of approximately 210 tandem repeats of a BamHI 2.2-kb sequence unit. Cytosines of the CpG dinucleotides of this region are extensively methylated on the two Z chromosomes in the male but much less methylated on the single Z chromosome in the female. The state of methylation of the MHM region is established after fertilization by about the 1-day embryonic stage. The MHM region is transcribed only in the female from the particular strand into heterogeneous, high molecular-mass, non-coding RNA, which is accumulated at the site of transcription, adjacent to the DMRT1 locus, in the nucleus. The transcriptional silence of the MHM region in the male is most likely caused by the CpG methylation, since treatment of the male embryonic fibroblasts with 5-azacytidine results in hypo-methylation and active transcription of this region. In ZZW triploid chickens, MHM regions are hypomethylated and transcribed on the two Z chromosomes, whereas MHM regions are hypermethylated and transcriptionally inactive on the three Z chromosomes in ZZZ triploid chickens, suggesting a possible role of the W chromosome on the state of the MHM region. |
| doi_str_mv | 10.1023/A:1009235120741 |
| format | Article |
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Cytosines of the CpG dinucleotides of this region are extensively methylated on the two Z chromosomes in the male but much less methylated on the single Z chromosome in the female. The state of methylation of the MHM region is established after fertilization by about the 1-day embryonic stage. The MHM region is transcribed only in the female from the particular strand into heterogeneous, high molecular-mass, non-coding RNA, which is accumulated at the site of transcription, adjacent to the DMRT1 locus, in the nucleus. The transcriptional silence of the MHM region in the male is most likely caused by the CpG methylation, since treatment of the male embryonic fibroblasts with 5-azacytidine results in hypo-methylation and active transcription of this region. In ZZW triploid chickens, MHM regions are hypomethylated and transcribed on the two Z chromosomes, whereas MHM regions are hypermethylated and transcriptionally inactive on the three Z chromosomes in ZZZ triploid chickens, suggesting a possible role of the W chromosome on the state of the MHM region.</description><identifier>ISSN: 0967-3849</identifier><identifier>EISSN: 1573-6849</identifier><identifier>DOI: 10.1023/A:1009235120741</identifier><identifier>PMID: 11321370</identifier><language>eng</language><publisher>Netherlands: Springer Nature B.V</publisher><subject>Animals ; Azacitidine - pharmacology ; Biotinylation ; Blotting, Northern ; Blotting, Southern ; Cell Nucleus - metabolism ; Chickens ; Chromosomes ; Chromosomes - ultrastructure ; CpG Islands ; Cytoplasm - metabolism ; DNA Methylation ; DNA Primers - metabolism ; DNA, Complementary - metabolism ; Female ; In Situ Hybridization, Fluorescence ; Male ; Ploidies ; RNA - metabolism ; RNA, Messenger - metabolism ; Sequence Analysis, DNA ; Sex Factors ; Transcription Factors - genetics ; Transcription, Genetic ; Transcriptional Activation</subject><ispartof>Chromosome research, 2001-01, Vol.9 (2), p.147-165</ispartof><rights>Kluwer Academic Publishers 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c312t-a862dc09c7e60aedb2d4941225ae5ce9bfcfd98c4890a43c442f012788b8cf763</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11321370$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Teranishi, M</creatorcontrib><creatorcontrib>Shimada, Y</creatorcontrib><creatorcontrib>Hori, T</creatorcontrib><creatorcontrib>Nakabayashi, O</creatorcontrib><creatorcontrib>Kikuchi, T</creatorcontrib><creatorcontrib>Macleod, T</creatorcontrib><creatorcontrib>Pym, R</creatorcontrib><creatorcontrib>Sheldon, B</creatorcontrib><creatorcontrib>Solovei, I</creatorcontrib><creatorcontrib>Macgregor, H</creatorcontrib><creatorcontrib>Mizuno, S</creatorcontrib><title>Transcripts of the MHM region on the chicken Z chromosome accumulate as non-coding RNA in the nucleus of female cells adjacent to the DMRT1 locus</title><title>Chromosome research</title><addtitle>Chromosome Res</addtitle><description>The male hypermethylated (MHM) region, located near the middle of the short arm of the Z chromosome of chickens, consists of approximately 210 tandem repeats of a BamHI 2.2-kb sequence unit. Cytosines of the CpG dinucleotides of this region are extensively methylated on the two Z chromosomes in the male but much less methylated on the single Z chromosome in the female. The state of methylation of the MHM region is established after fertilization by about the 1-day embryonic stage. The MHM region is transcribed only in the female from the particular strand into heterogeneous, high molecular-mass, non-coding RNA, which is accumulated at the site of transcription, adjacent to the DMRT1 locus, in the nucleus. The transcriptional silence of the MHM region in the male is most likely caused by the CpG methylation, since treatment of the male embryonic fibroblasts with 5-azacytidine results in hypo-methylation and active transcription of this region. In ZZW triploid chickens, MHM regions are hypomethylated and transcribed on the two Z chromosomes, whereas MHM regions are hypermethylated and transcriptionally inactive on the three Z chromosomes in ZZZ triploid chickens, suggesting a possible role of the W chromosome on the state of the MHM region.</description><subject>Animals</subject><subject>Azacitidine - pharmacology</subject><subject>Biotinylation</subject><subject>Blotting, Northern</subject><subject>Blotting, Southern</subject><subject>Cell Nucleus - metabolism</subject><subject>Chickens</subject><subject>Chromosomes</subject><subject>Chromosomes - ultrastructure</subject><subject>CpG Islands</subject><subject>Cytoplasm - metabolism</subject><subject>DNA Methylation</subject><subject>DNA Primers - metabolism</subject><subject>DNA, Complementary - metabolism</subject><subject>Female</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Male</subject><subject>Ploidies</subject><subject>RNA - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Analysis, DNA</subject><subject>Sex Factors</subject><subject>Transcription Factors - genetics</subject><subject>Transcription, Genetic</subject><subject>Transcriptional Activation</subject><issn>0967-3849</issn><issn>1573-6849</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kUtLxDAQx4Mouj7O3iR40FM1rzaJt2V9gqsg68VLyU5T7doma9Mc_Bh-Y7M-Lh6EgfkTfvNjhiC0T8kJJYyfjs8oIZrxnDIiBV1DI5pLnhVK6HU0IrqQGU95C22HsCCEKC7oJtqilDPKJRmhj1lvXIC-WQ4B-xoPLxZPr6e4t8-NdzjV6gVeGni1Dj-l1PvOB99ZbABiF1szpBiw8y4DXzXuGT_cjXHzPegitDZ-mWvbmTapbNsGbKqFAesGPPgv7nz6MKO49RDDLtqoTRvs3k_fQY-XF7PJdXZ7f3UzGd9mwCkbMqMKVgHRIG1BjK3mrBJaUMZyY3Owel5DXWkFQmliBAchWE0ok0rNFdSy4Dvo-Nu77P1btGEouyastjPO-hhKWTBWaM1VIo_-J4lUybsCD_-ACx97l64oZU5yJZSQCTr4geK8s1W57JvO9O_l75_wTy-mjYk</recordid><startdate>20010101</startdate><enddate>20010101</enddate><creator>Teranishi, M</creator><creator>Shimada, Y</creator><creator>Hori, T</creator><creator>Nakabayashi, O</creator><creator>Kikuchi, T</creator><creator>Macleod, T</creator><creator>Pym, R</creator><creator>Sheldon, B</creator><creator>Solovei, I</creator><creator>Macgregor, H</creator><creator>Mizuno, S</creator><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20010101</creationdate><title>Transcripts of the MHM region on the chicken Z chromosome accumulate as non-coding RNA in the nucleus of female cells adjacent to the DMRT1 locus</title><author>Teranishi, M ; Shimada, Y ; Hori, T ; Nakabayashi, O ; Kikuchi, T ; Macleod, T ; Pym, R ; Sheldon, B ; Solovei, I ; Macgregor, H ; Mizuno, S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c312t-a862dc09c7e60aedb2d4941225ae5ce9bfcfd98c4890a43c442f012788b8cf763</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Azacitidine - pharmacology</topic><topic>Biotinylation</topic><topic>Blotting, Northern</topic><topic>Blotting, Southern</topic><topic>Cell Nucleus - metabolism</topic><topic>Chickens</topic><topic>Chromosomes</topic><topic>Chromosomes - ultrastructure</topic><topic>CpG Islands</topic><topic>Cytoplasm - metabolism</topic><topic>DNA Methylation</topic><topic>DNA Primers - metabolism</topic><topic>DNA, Complementary - metabolism</topic><topic>Female</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Male</topic><topic>Ploidies</topic><topic>RNA - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Analysis, DNA</topic><topic>Sex Factors</topic><topic>Transcription Factors - genetics</topic><topic>Transcription, Genetic</topic><topic>Transcriptional Activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Teranishi, M</creatorcontrib><creatorcontrib>Shimada, Y</creatorcontrib><creatorcontrib>Hori, T</creatorcontrib><creatorcontrib>Nakabayashi, O</creatorcontrib><creatorcontrib>Kikuchi, T</creatorcontrib><creatorcontrib>Macleod, T</creatorcontrib><creatorcontrib>Pym, R</creatorcontrib><creatorcontrib>Sheldon, B</creatorcontrib><creatorcontrib>Solovei, I</creatorcontrib><creatorcontrib>Macgregor, H</creatorcontrib><creatorcontrib>Mizuno, S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chromosome research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teranishi, M</au><au>Shimada, Y</au><au>Hori, T</au><au>Nakabayashi, O</au><au>Kikuchi, T</au><au>Macleod, T</au><au>Pym, R</au><au>Sheldon, B</au><au>Solovei, I</au><au>Macgregor, H</au><au>Mizuno, S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcripts of the MHM region on the chicken Z chromosome accumulate as non-coding RNA in the nucleus of female cells adjacent to the DMRT1 locus</atitle><jtitle>Chromosome research</jtitle><addtitle>Chromosome Res</addtitle><date>2001-01-01</date><risdate>2001</risdate><volume>9</volume><issue>2</issue><spage>147</spage><epage>165</epage><pages>147-165</pages><issn>0967-3849</issn><eissn>1573-6849</eissn><abstract>The male hypermethylated (MHM) region, located near the middle of the short arm of the Z chromosome of chickens, consists of approximately 210 tandem repeats of a BamHI 2.2-kb sequence unit. Cytosines of the CpG dinucleotides of this region are extensively methylated on the two Z chromosomes in the male but much less methylated on the single Z chromosome in the female. The state of methylation of the MHM region is established after fertilization by about the 1-day embryonic stage. The MHM region is transcribed only in the female from the particular strand into heterogeneous, high molecular-mass, non-coding RNA, which is accumulated at the site of transcription, adjacent to the DMRT1 locus, in the nucleus. The transcriptional silence of the MHM region in the male is most likely caused by the CpG methylation, since treatment of the male embryonic fibroblasts with 5-azacytidine results in hypo-methylation and active transcription of this region. In ZZW triploid chickens, MHM regions are hypomethylated and transcribed on the two Z chromosomes, whereas MHM regions are hypermethylated and transcriptionally inactive on the three Z chromosomes in ZZZ triploid chickens, suggesting a possible role of the W chromosome on the state of the MHM region.</abstract><cop>Netherlands</cop><pub>Springer Nature B.V</pub><pmid>11321370</pmid><doi>10.1023/A:1009235120741</doi><tpages>19</tpages></addata></record> |
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| subjects | Animals Azacitidine - pharmacology Biotinylation Blotting, Northern Blotting, Southern Cell Nucleus - metabolism Chickens Chromosomes Chromosomes - ultrastructure CpG Islands Cytoplasm - metabolism DNA Methylation DNA Primers - metabolism DNA, Complementary - metabolism Female In Situ Hybridization, Fluorescence Male Ploidies RNA - metabolism RNA, Messenger - metabolism Sequence Analysis, DNA Sex Factors Transcription Factors - genetics Transcription, Genetic Transcriptional Activation |
| title | Transcripts of the MHM region on the chicken Z chromosome accumulate as non-coding RNA in the nucleus of female cells adjacent to the DMRT1 locus |
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