DNA oligonucleotide microarray technology identifies fisp-12 among other potential fibrogenic genes following murine unilateral ureteral obstruction (UUO): Modulation during epithelial-mesenchymal transition
DNA oligonucleotide microarray technology identifies fisp-12 among other potential fibrogenic genes following murine unilateral ureteral obstruction (UUO): Modulation during epithelial-mesenchymal transition. Tubulointerstitial inflammation and fibrosis are pathologic hallmarks of end-stage renal di...
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| Veröffentlicht in: | Kidney international 2003-12, Vol.64 (6), p.2079-2091 |
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| creator | Higgins, Debra F. Lappin, David W.P. Kieran, Niamh E. Anders, Hans J. Watson, Ronald W.G. Strutz, Frank Schlondorff, Detlef Haase, Volker H. Fitzpatrick, John M. Godson, Catherine Brady, Hugh R. |
| description | DNA oligonucleotide microarray technology identifies fisp-12 among other potential fibrogenic genes following murine unilateral ureteral obstruction (UUO): Modulation during epithelial-mesenchymal transition.
Tubulointerstitial inflammation and fibrosis are pathologic hallmarks of end-stage renal disease (ESRD). Here we have used DNA microarray technology to monitor the transcriptomic responses to murine unilateral ureteral obstruction (UUO) with a view to identifying molecular modulators of tubulointerstitial fibrosis.
Using Affymetrix Mu74Av2 microarrays, gene expression 4 and 10days postobstruction was investigated relative to control contralateral kidneys. Candidate profibrogenic genes were further investigated in epithelial cells undergoing epithelial to mesenchymal transition (EMT) in vitro.
mRNA levels for 1091 gene/EST sequences, of a total of 12,488 displayed on the microarray, were altered twofold or greater by days 4 and 10 postobstruction compared to contralateral control kidneys. Genes were categorised into functional groups, including modulators of cytoskeletal and extracellular matrix metabolism, cell growth, signalling, and transcription/translational events. Among the potentially profibrogenic genes, whose mRNA levels were increased after UUO, were fibroblast-inducible secreted protein (fisp-12), the murine homologue of connective tissue growth factor (CTGF), collagen XVIIIα1, secreted protein acidic and rich in cysteine (SPARC), and src-suppressed C-kinase substrate (SSeCKS). A sustained increase in fisp-12 mRNA level was observed during EMT induced by transforming growth factor-β1 (TGF-β1) and epidermal growth factor (EGF).
Altered gene expression in murine UUO has been demonstrated. Increased expression of fisp-12, SPARC, and SSeCKS has been shown in response to TGF-β1 treatment and during EMT, suggesting that these genes may offer potential therapeutic targets against tubulointerstitial fibrosis. |
| doi_str_mv | 10.1046/j.1523-1755.2003.00306.x |
| format | Article |
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Tubulointerstitial inflammation and fibrosis are pathologic hallmarks of end-stage renal disease (ESRD). Here we have used DNA microarray technology to monitor the transcriptomic responses to murine unilateral ureteral obstruction (UUO) with a view to identifying molecular modulators of tubulointerstitial fibrosis.
Using Affymetrix Mu74Av2 microarrays, gene expression 4 and 10days postobstruction was investigated relative to control contralateral kidneys. Candidate profibrogenic genes were further investigated in epithelial cells undergoing epithelial to mesenchymal transition (EMT) in vitro.
mRNA levels for 1091 gene/EST sequences, of a total of 12,488 displayed on the microarray, were altered twofold or greater by days 4 and 10 postobstruction compared to contralateral control kidneys. Genes were categorised into functional groups, including modulators of cytoskeletal and extracellular matrix metabolism, cell growth, signalling, and transcription/translational events. Among the potentially profibrogenic genes, whose mRNA levels were increased after UUO, were fibroblast-inducible secreted protein (fisp-12), the murine homologue of connective tissue growth factor (CTGF), collagen XVIIIα1, secreted protein acidic and rich in cysteine (SPARC), and src-suppressed C-kinase substrate (SSeCKS). A sustained increase in fisp-12 mRNA level was observed during EMT induced by transforming growth factor-β1 (TGF-β1) and epidermal growth factor (EGF).
Altered gene expression in murine UUO has been demonstrated. Increased expression of fisp-12, SPARC, and SSeCKS has been shown in response to TGF-β1 treatment and during EMT, suggesting that these genes may offer potential therapeutic targets against tubulointerstitial fibrosis.</description><identifier>ISSN: 0085-2538</identifier><identifier>EISSN: 1523-1755</identifier><identifier>DOI: 10.1046/j.1523-1755.2003.00306.x</identifier><identifier>PMID: 14633130</identifier><identifier>CODEN: KDYIA5</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>A Kinase Anchor Proteins ; Animals ; Biological and medical sciences ; Cell Cycle Proteins - genetics ; Cells, Cultured ; Collagen Type XVIII - genetics ; collagen XVIIIα1 ; Connective Tissue Growth Factor ; Disease Progression ; Epidermal Growth Factor - pharmacology ; fisp-12 ; Gene Expression ; Immediate-Early Proteins - genetics ; Immediate-Early Proteins - metabolism ; Intercellular Signaling Peptides and Proteins - genetics ; Intercellular Signaling Peptides and Proteins - metabolism ; Interstitial nephritis ; Medical sciences ; Mice ; Mice, Inbred C57BL ; microarray analysis ; Mitogens - genetics ; Nephrology. Urinary tract diseases ; Nephropathies. Renovascular diseases. Renal failure ; Oligonucleotide Array Sequence Analysis ; Osteonectin - genetics ; profibrogenic genes ; Protein Isoforms - genetics ; renal fibrosis ; RNA, Messenger - metabolism ; SPARC ; SSeCKS ; Transforming Growth Factor beta - pharmacology ; Transforming Growth Factor beta1 ; Ureteral Obstruction - genetics ; Ureteral Obstruction - metabolism ; Ureteral Obstruction - pathology ; Ureteral Obstruction - physiopathology ; UUO</subject><ispartof>Kidney international, 2003-12, Vol.64 (6), p.2079-2091</ispartof><rights>2003 International Society of Nephrology</rights><rights>2004 INIST-CNRS</rights><rights>Copyright Nature Publishing Group Dec 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c606t-1808a5e404a72f781dedc2843a2f29e4a8a3e90c693cb082f20b51578b1a6d013</citedby><cites>FETCH-LOGICAL-c606t-1808a5e404a72f781dedc2843a2f29e4a8a3e90c693cb082f20b51578b1a6d013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15288796$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/14633130$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Higgins, Debra F.</creatorcontrib><creatorcontrib>Lappin, David W.P.</creatorcontrib><creatorcontrib>Kieran, Niamh E.</creatorcontrib><creatorcontrib>Anders, Hans J.</creatorcontrib><creatorcontrib>Watson, Ronald W.G.</creatorcontrib><creatorcontrib>Strutz, Frank</creatorcontrib><creatorcontrib>Schlondorff, Detlef</creatorcontrib><creatorcontrib>Haase, Volker H.</creatorcontrib><creatorcontrib>Fitzpatrick, John M.</creatorcontrib><creatorcontrib>Godson, Catherine</creatorcontrib><creatorcontrib>Brady, Hugh R.</creatorcontrib><title>DNA oligonucleotide microarray technology identifies fisp-12 among other potential fibrogenic genes following murine unilateral ureteral obstruction (UUO): Modulation during epithelial-mesenchymal transition</title><title>Kidney international</title><addtitle>Kidney Int</addtitle><description>DNA oligonucleotide microarray technology identifies fisp-12 among other potential fibrogenic genes following murine unilateral ureteral obstruction (UUO): Modulation during epithelial-mesenchymal transition.
Tubulointerstitial inflammation and fibrosis are pathologic hallmarks of end-stage renal disease (ESRD). Here we have used DNA microarray technology to monitor the transcriptomic responses to murine unilateral ureteral obstruction (UUO) with a view to identifying molecular modulators of tubulointerstitial fibrosis.
Using Affymetrix Mu74Av2 microarrays, gene expression 4 and 10days postobstruction was investigated relative to control contralateral kidneys. Candidate profibrogenic genes were further investigated in epithelial cells undergoing epithelial to mesenchymal transition (EMT) in vitro.
mRNA levels for 1091 gene/EST sequences, of a total of 12,488 displayed on the microarray, were altered twofold or greater by days 4 and 10 postobstruction compared to contralateral control kidneys. Genes were categorised into functional groups, including modulators of cytoskeletal and extracellular matrix metabolism, cell growth, signalling, and transcription/translational events. Among the potentially profibrogenic genes, whose mRNA levels were increased after UUO, were fibroblast-inducible secreted protein (fisp-12), the murine homologue of connective tissue growth factor (CTGF), collagen XVIIIα1, secreted protein acidic and rich in cysteine (SPARC), and src-suppressed C-kinase substrate (SSeCKS). A sustained increase in fisp-12 mRNA level was observed during EMT induced by transforming growth factor-β1 (TGF-β1) and epidermal growth factor (EGF).
Altered gene expression in murine UUO has been demonstrated. Increased expression of fisp-12, SPARC, and SSeCKS has been shown in response to TGF-β1 treatment and during EMT, suggesting that these genes may offer potential therapeutic targets against tubulointerstitial fibrosis.</description><subject>A Kinase Anchor Proteins</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Cycle Proteins - genetics</subject><subject>Cells, Cultured</subject><subject>Collagen Type XVIII - genetics</subject><subject>collagen XVIIIα1</subject><subject>Connective Tissue Growth Factor</subject><subject>Disease Progression</subject><subject>Epidermal Growth Factor - pharmacology</subject><subject>fisp-12</subject><subject>Gene Expression</subject><subject>Immediate-Early Proteins - genetics</subject><subject>Immediate-Early Proteins - metabolism</subject><subject>Intercellular Signaling Peptides and Proteins - genetics</subject><subject>Intercellular Signaling Peptides and Proteins - metabolism</subject><subject>Interstitial nephritis</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>microarray analysis</subject><subject>Mitogens - genetics</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Nephropathies. Renovascular diseases. Renal failure</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Osteonectin - genetics</subject><subject>profibrogenic genes</subject><subject>Protein Isoforms - genetics</subject><subject>renal fibrosis</subject><subject>RNA, Messenger - metabolism</subject><subject>SPARC</subject><subject>SSeCKS</subject><subject>Transforming Growth Factor beta - pharmacology</subject><subject>Transforming Growth Factor beta1</subject><subject>Ureteral Obstruction - genetics</subject><subject>Ureteral Obstruction - metabolism</subject><subject>Ureteral Obstruction - pathology</subject><subject>Ureteral Obstruction - physiopathology</subject><subject>UUO</subject><issn>0085-2538</issn><issn>1523-1755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFksmOEzEQhlsIxISBV0AWEtuhg5deHG7DsEoDcyFny-2uThy57WC7YfKUvBLVJGIkDmDJW9VXdtn1FwVhdMlo1bzaLVnNRcnaul5ySsUSO22WN3eKxR_H3WJBqaxLXgt5VjxIaUdxvxL0fnHGqkYIJuii-Pn2ywUJzm6Cn4yDkG0PZLQmBh2jPpAMZuuDC5sDQY_PdrCQyGDTvmSc6DH4DQl5C5HsQ5792qG3i2ED3hqC44wH58IPi-g4ReuBTN46nSEiPEU4LkKXcpxMtsGTF-v19cvX5HPoJ-RmSz8HbgjsLV7m8JZyhATebA8jxuaofbIz-LC4N2iX4NFpPi_W7999vfxYXl1_-HR5cVWahja5ZJJKXUNFK93yoZWsh95wWQnNB76CSkstYEVNsxKmoxKNtKtZ3cqO6aanTJwXz4_n7mP4NkHKarTJgHPaQ5iSahvOGyl4heSzf5OswjKxBsEnf4G7MEWPr1CcUVZjaxGSRwgrlFKEQe2jHXU8KEbVrA21U7ME1CwBNWtD_daGusHQx6fzp26E_jbwJAYEnp4AnYx2A36qsemWq7mU7WpO9M2RA_zg7xaiSsZiLaC3EUxWfbD_z-YXxC7c3g</recordid><startdate>20031201</startdate><enddate>20031201</enddate><creator>Higgins, Debra F.</creator><creator>Lappin, David W.P.</creator><creator>Kieran, Niamh E.</creator><creator>Anders, Hans J.</creator><creator>Watson, Ronald W.G.</creator><creator>Strutz, Frank</creator><creator>Schlondorff, Detlef</creator><creator>Haase, Volker H.</creator><creator>Fitzpatrick, John M.</creator><creator>Godson, Catherine</creator><creator>Brady, Hugh R.</creator><general>Elsevier Inc</general><general>Nature Publishing</general><general>Elsevier Limited</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>7TM</scope></search><sort><creationdate>20031201</creationdate><title>DNA oligonucleotide microarray technology identifies fisp-12 among other potential fibrogenic genes following murine unilateral ureteral obstruction (UUO): Modulation during epithelial-mesenchymal transition</title><author>Higgins, Debra F. ; Lappin, David W.P. ; Kieran, Niamh E. ; Anders, Hans J. ; Watson, Ronald W.G. ; Strutz, Frank ; Schlondorff, Detlef ; Haase, Volker H. ; Fitzpatrick, John M. ; Godson, Catherine ; Brady, Hugh R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c606t-1808a5e404a72f781dedc2843a2f29e4a8a3e90c693cb082f20b51578b1a6d013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>A Kinase Anchor Proteins</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Cycle Proteins - genetics</topic><topic>Cells, Cultured</topic><topic>Collagen Type XVIII - genetics</topic><topic>collagen XVIIIα1</topic><topic>Connective Tissue Growth Factor</topic><topic>Disease Progression</topic><topic>Epidermal Growth Factor - pharmacology</topic><topic>fisp-12</topic><topic>Gene Expression</topic><topic>Immediate-Early Proteins - genetics</topic><topic>Immediate-Early Proteins - metabolism</topic><topic>Intercellular Signaling Peptides and Proteins - genetics</topic><topic>Intercellular Signaling Peptides and Proteins - metabolism</topic><topic>Interstitial nephritis</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>microarray analysis</topic><topic>Mitogens - genetics</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Nephropathies. Renovascular diseases. Renal failure</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Osteonectin - genetics</topic><topic>profibrogenic genes</topic><topic>Protein Isoforms - genetics</topic><topic>renal fibrosis</topic><topic>RNA, Messenger - metabolism</topic><topic>SPARC</topic><topic>SSeCKS</topic><topic>Transforming Growth Factor beta - pharmacology</topic><topic>Transforming Growth Factor beta1</topic><topic>Ureteral Obstruction - genetics</topic><topic>Ureteral Obstruction - metabolism</topic><topic>Ureteral Obstruction - pathology</topic><topic>Ureteral Obstruction - physiopathology</topic><topic>UUO</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Higgins, Debra F.</creatorcontrib><creatorcontrib>Lappin, David W.P.</creatorcontrib><creatorcontrib>Kieran, Niamh E.</creatorcontrib><creatorcontrib>Anders, Hans J.</creatorcontrib><creatorcontrib>Watson, Ronald W.G.</creatorcontrib><creatorcontrib>Strutz, Frank</creatorcontrib><creatorcontrib>Schlondorff, Detlef</creatorcontrib><creatorcontrib>Haase, Volker H.</creatorcontrib><creatorcontrib>Fitzpatrick, John M.</creatorcontrib><creatorcontrib>Godson, Catherine</creatorcontrib><creatorcontrib>Brady, Hugh R.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Kidney international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Higgins, Debra F.</au><au>Lappin, David W.P.</au><au>Kieran, Niamh E.</au><au>Anders, Hans J.</au><au>Watson, Ronald W.G.</au><au>Strutz, Frank</au><au>Schlondorff, Detlef</au><au>Haase, Volker H.</au><au>Fitzpatrick, John M.</au><au>Godson, Catherine</au><au>Brady, Hugh R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA oligonucleotide microarray technology identifies fisp-12 among other potential fibrogenic genes following murine unilateral ureteral obstruction (UUO): Modulation during epithelial-mesenchymal transition</atitle><jtitle>Kidney international</jtitle><addtitle>Kidney Int</addtitle><date>2003-12-01</date><risdate>2003</risdate><volume>64</volume><issue>6</issue><spage>2079</spage><epage>2091</epage><pages>2079-2091</pages><issn>0085-2538</issn><eissn>1523-1755</eissn><coden>KDYIA5</coden><abstract>DNA oligonucleotide microarray technology identifies fisp-12 among other potential fibrogenic genes following murine unilateral ureteral obstruction (UUO): Modulation during epithelial-mesenchymal transition.
Tubulointerstitial inflammation and fibrosis are pathologic hallmarks of end-stage renal disease (ESRD). Here we have used DNA microarray technology to monitor the transcriptomic responses to murine unilateral ureteral obstruction (UUO) with a view to identifying molecular modulators of tubulointerstitial fibrosis.
Using Affymetrix Mu74Av2 microarrays, gene expression 4 and 10days postobstruction was investigated relative to control contralateral kidneys. Candidate profibrogenic genes were further investigated in epithelial cells undergoing epithelial to mesenchymal transition (EMT) in vitro.
mRNA levels for 1091 gene/EST sequences, of a total of 12,488 displayed on the microarray, were altered twofold or greater by days 4 and 10 postobstruction compared to contralateral control kidneys. Genes were categorised into functional groups, including modulators of cytoskeletal and extracellular matrix metabolism, cell growth, signalling, and transcription/translational events. Among the potentially profibrogenic genes, whose mRNA levels were increased after UUO, were fibroblast-inducible secreted protein (fisp-12), the murine homologue of connective tissue growth factor (CTGF), collagen XVIIIα1, secreted protein acidic and rich in cysteine (SPARC), and src-suppressed C-kinase substrate (SSeCKS). A sustained increase in fisp-12 mRNA level was observed during EMT induced by transforming growth factor-β1 (TGF-β1) and epidermal growth factor (EGF).
Altered gene expression in murine UUO has been demonstrated. Increased expression of fisp-12, SPARC, and SSeCKS has been shown in response to TGF-β1 treatment and during EMT, suggesting that these genes may offer potential therapeutic targets against tubulointerstitial fibrosis.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>14633130</pmid><doi>10.1046/j.1523-1755.2003.00306.x</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Kidney international, 2003-12, Vol.64 (6), p.2079-2091 |
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| subjects | A Kinase Anchor Proteins Animals Biological and medical sciences Cell Cycle Proteins - genetics Cells, Cultured Collagen Type XVIII - genetics collagen XVIIIα1 Connective Tissue Growth Factor Disease Progression Epidermal Growth Factor - pharmacology fisp-12 Gene Expression Immediate-Early Proteins - genetics Immediate-Early Proteins - metabolism Intercellular Signaling Peptides and Proteins - genetics Intercellular Signaling Peptides and Proteins - metabolism Interstitial nephritis Medical sciences Mice Mice, Inbred C57BL microarray analysis Mitogens - genetics Nephrology. Urinary tract diseases Nephropathies. Renovascular diseases. Renal failure Oligonucleotide Array Sequence Analysis Osteonectin - genetics profibrogenic genes Protein Isoforms - genetics renal fibrosis RNA, Messenger - metabolism SPARC SSeCKS Transforming Growth Factor beta - pharmacology Transforming Growth Factor beta1 Ureteral Obstruction - genetics Ureteral Obstruction - metabolism Ureteral Obstruction - pathology Ureteral Obstruction - physiopathology UUO |
| title | DNA oligonucleotide microarray technology identifies fisp-12 among other potential fibrogenic genes following murine unilateral ureteral obstruction (UUO): Modulation during epithelial-mesenchymal transition |
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