Expression and SNP association analysis of porcine FBXL4 gene

As a kind of E3 ligase, the product of FBXL4 gene belongs to a member of FBLs which is the biggest eukaryotic subfamily of F-BOX proteins, it can recognize some substrate through particular protein–protein interaction domains. To investigate its functions, the polymorphism and association analysis w...

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Veröffentlicht in:Molecular biology reports 2010, Vol.37 (1), p.579-585
Hauptverfasser: Li, Y., Yang, S. L., Tang, Z. L., Cui, W. T., Mu, Y. L., Chu, M. X., Zhao, S. H., Wu, Z. F., Li, K., Peng, K. M.
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container_end_page 585
container_issue 1
container_start_page 579
container_title Molecular biology reports
container_volume 37
creator Li, Y.
Yang, S. L.
Tang, Z. L.
Cui, W. T.
Mu, Y. L.
Chu, M. X.
Zhao, S. H.
Wu, Z. F.
Li, K.
Peng, K. M.
description As a kind of E3 ligase, the product of FBXL4 gene belongs to a member of FBLs which is the biggest eukaryotic subfamily of F-BOX proteins, it can recognize some substrate through particular protein–protein interaction domains. To investigate its functions, the polymorphism and association analysis was analyzed. The partial cDNA of porcine FBXL4 with 2384 bp long was first cloned; the deduced protein comprises a conserved F-BOX domain at position from the 277th to 332nd amino acid. The phylogenetic tree indicated porcine FBXL4 has the closest genetic relationship with bovine FBXL4 than other selected animal species. Ten tissue expression level of porcine FBXL4 mRNA fluctuated remarkably in a large range by quantitative RT-PCR analysis. For two identified SNPs, the genotyping analysis of Tail showed TT genotype owned dominance in introduced Landrace pig and miniature Guizhou and Wuzhishan breeds, but CC genotype was more than two other genotypes in miniature Laiwu breed. While in another genotyping analysis of BsaJI, CC genotype was obviously more than other genotypes in two kinds of Chinese miniature pig breeds and introduced Landrace pig breeds. Furthermore, the association analysis with immune traits and blood parameters revealed that SNP Tail was significantly associated with the lymphocyte percentage ( P  = 0.0166) and the antibody levels for pseudorabies virus vaccination ( P  = 0.0001) of neonate piglets at 0 day. Meanwhile, SNP BsaJI was significantly associated with lymphocyte percentage of individuals at 32 days ( P  = 0.0351), neutrophil percentage ( P  = 0.0005), the absolute lymphocyte count ( P  = 0.0458), and the mixed cells ( P  = 0.0010) of neonate piglets at 0 day.
doi_str_mv 10.1007/s11033-009-9825-7
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L. ; Tang, Z. L. ; Cui, W. T. ; Mu, Y. L. ; Chu, M. X. ; Zhao, S. H. ; Wu, Z. F. ; Li, K. ; Peng, K. M.</creator><creatorcontrib>Li, Y. ; Yang, S. L. ; Tang, Z. L. ; Cui, W. T. ; Mu, Y. L. ; Chu, M. X. ; Zhao, S. H. ; Wu, Z. F. ; Li, K. ; Peng, K. M.</creatorcontrib><description>As a kind of E3 ligase, the product of FBXL4 gene belongs to a member of FBLs which is the biggest eukaryotic subfamily of F-BOX proteins, it can recognize some substrate through particular protein–protein interaction domains. To investigate its functions, the polymorphism and association analysis was analyzed. The partial cDNA of porcine FBXL4 with 2384 bp long was first cloned; the deduced protein comprises a conserved F-BOX domain at position from the 277th to 332nd amino acid. The phylogenetic tree indicated porcine FBXL4 has the closest genetic relationship with bovine FBXL4 than other selected animal species. 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L.</au><au>Tang, Z. L.</au><au>Cui, W. T.</au><au>Mu, Y. L.</au><au>Chu, M. X.</au><au>Zhao, S. H.</au><au>Wu, Z. F.</au><au>Li, K.</au><au>Peng, K. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression and SNP association analysis of porcine FBXL4 gene</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2010</date><risdate>2010</risdate><volume>37</volume><issue>1</issue><spage>579</spage><epage>585</epage><pages>579-585</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>As a kind of E3 ligase, the product of FBXL4 gene belongs to a member of FBLs which is the biggest eukaryotic subfamily of F-BOX proteins, it can recognize some substrate through particular protein–protein interaction domains. To investigate its functions, the polymorphism and association analysis was analyzed. The partial cDNA of porcine FBXL4 with 2384 bp long was first cloned; the deduced protein comprises a conserved F-BOX domain at position from the 277th to 332nd amino acid. The phylogenetic tree indicated porcine FBXL4 has the closest genetic relationship with bovine FBXL4 than other selected animal species. Ten tissue expression level of porcine FBXL4 mRNA fluctuated remarkably in a large range by quantitative RT-PCR analysis. For two identified SNPs, the genotyping analysis of Tail showed TT genotype owned dominance in introduced Landrace pig and miniature Guizhou and Wuzhishan breeds, but CC genotype was more than two other genotypes in miniature Laiwu breed. While in another genotyping analysis of BsaJI, CC genotype was obviously more than other genotypes in two kinds of Chinese miniature pig breeds and introduced Landrace pig breeds. Furthermore, the association analysis with immune traits and blood parameters revealed that SNP Tail was significantly associated with the lymphocyte percentage ( P  = 0.0166) and the antibody levels for pseudorabies virus vaccination ( P  = 0.0001) of neonate piglets at 0 day. Meanwhile, SNP BsaJI was significantly associated with lymphocyte percentage of individuals at 32 days ( P  = 0.0351), neutrophil percentage ( P  = 0.0005), the absolute lymphocyte count ( P  = 0.0458), and the mixed cells ( P  = 0.0010) of neonate piglets at 0 day.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>19768576</pmid><doi>10.1007/s11033-009-9825-7</doi><tpages>7</tpages></addata></record>
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subjects Amino acids
Animal Anatomy
Animal Biochemistry
Animals
Association analysis
Biomedical and Life Sciences
Dominance
F-Box Proteins - genetics
Gene expression
Gene Expression Profiling
Gene Expression Regulation
Gene Frequency - genetics
Gene polymorphism
Genotype
Genotype & phenotype
Genotyping
Histology
Hogs
Introns - genetics
Life Sciences
Lymphocytes
Molecular biology
Molecular Sequence Data
Morphology
Neonates
Organ Specificity - genetics
Phylogeny
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polymorphism, Single Nucleotide - genetics
Proteins
Pseudorabies virus
RNA, Messenger - genetics
RNA, Messenger - metabolism
Single-nucleotide polymorphism
Sus scrofa - blood
Sus scrofa - genetics
Sus scrofa - immunology
Tails
title Expression and SNP association analysis of porcine FBXL4 gene
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