Characterization of anti-channel catfish IgL Ief monoclonal antibodies
This study characterizes three monoclonal antibodies (mAbs) developed against the constant (C) region of the immunoglobulin light (IgL) Ief chain isotype of the channel catfish, Ictalurus punctatus. Microsphere bead assays and Western blot analyses utilizing different recombinant (r) proteins show t...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 2010-06, Vol.135 (3-4), p.325-328 |
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| container_issue | 3-4 |
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| container_title | Veterinary immunology and immunopathology |
| container_volume | 135 |
| creator | Edholm, Eva-Stina Hudgens, Edward D Tompkins, Dannielle Sahoo, Manoranjan Burkhalter, Bailey Miller, Norman W Bengten, Eva Wilson, Melanie |
| description | This study characterizes three monoclonal antibodies (mAbs) developed against the constant (C) region of the immunoglobulin light (IgL) Ief chain isotype of the channel catfish, Ictalurus punctatus. Microsphere bead assays and Western blot analyses utilizing different recombinant (r) proteins show that these anti-catfish IgL Ief chain mAbs each specifically recognize the denatured form of IgL Ief. Importantly, Western blotting of catfish sera using the anti-IgL Ief mAbs also identified an IgL chain-sized immunoreactive band(s) of 27kDa. It is anticipated that these mAbs in combination with the already existing anti-catfish Ig heavy (H) and IgL chain mAbs will be useful in future studies examining the functional roles of the different catfish IgL isotypes. |
| doi_str_mv | 10.1016/j.vetimm.2010.01.004 |
| format | Article |
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Microsphere bead assays and Western blot analyses utilizing different recombinant (r) proteins show that these anti-catfish IgL Ief chain mAbs each specifically recognize the denatured form of IgL Ief. Importantly, Western blotting of catfish sera using the anti-IgL Ief mAbs also identified an IgL chain-sized immunoreactive band(s) of 27kDa. 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| fulltext | fulltext |
| identifier | ISSN: 0165-2427 |
| ispartof | Veterinary immunology and immunopathology, 2010-06, Vol.135 (3-4), p.325-328 |
| issn | 0165-2427 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_762264929 |
| source | Elsevier ScienceDirect Journals |
| subjects | Freshwater Ictalurus punctatus |
| title | Characterization of anti-channel catfish IgL Ief monoclonal antibodies |
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