Detection of Immunoreactive Schizophyllan by Solid-Phase Enzyme-Linked Immunosorbent Assay
In order to detect immunoreactive schizophyllan (SPG), one of the β-1, 3-glucans in solution, a murine antischizophyllan monoclonal antibody, SPG1-HS, was used as a primary antibody for solid-phase enzyme-linked immunosorbent assay (ELISA). Polysaccharide-bovine serum albumin (BSA) and polysaccharid...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 1993/11/15, Vol.16(11), pp.1091-1093 |
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| container_title | Biological & pharmaceutical bulletin |
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| creator | HIRATA, Akio ITOH, Wataru KOMODA, Makiko TABATA, Kengo ITOYAMA, Shinji SUGAWARA, Isamu |
| description | In order to detect immunoreactive schizophyllan (SPG), one of the β-1, 3-glucans in solution, a murine antischizophyllan monoclonal antibody, SPG1-HS, was used as a primary antibody for solid-phase enzyme-linked immunosorbent assay (ELISA). Polysaccharide-bovine serum albumin (BSA) and polysaccharide-bovine hemoglobin (Hb) conjugate were prepared as an antigen. The absorbance at 490 nm in ELISA was directly proportional to the SPG concentration in SPG-BSA or SPG-Hb conjugates, allowing the accurate measurement of SPG at concentrations of more than 1.0 μg/ml. Also, this ELISA detected SPG in SPG-BSA and SPG-Hb conjugates in human serum in a similar manner. These results suggest that this ELISA is applicable for the determination of SPG concentration in SPG-BSA and SPG-Hb conjugates in human tissue and blood. |
| doi_str_mv | 10.1248/bpb.16.1091 |
| format | Article |
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Polysaccharide-bovine serum albumin (BSA) and polysaccharide-bovine hemoglobin (Hb) conjugate were prepared as an antigen. The absorbance at 490 nm in ELISA was directly proportional to the SPG concentration in SPG-BSA or SPG-Hb conjugates, allowing the accurate measurement of SPG at concentrations of more than 1.0 μg/ml. Also, this ELISA detected SPG in SPG-BSA and SPG-Hb conjugates in human serum in a similar manner. These results suggest that this ELISA is applicable for the determination of SPG concentration in SPG-BSA and SPG-Hb conjugates in human tissue and blood.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.16.1091</identifier><identifier>PMID: 8312861</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>Analysis ; Animals ; Antibodies, Monoclonal ; Biological and medical sciences ; Enzyme-Linked Immunosorbent Assay ; General pharmacology ; Humans ; Medical sciences ; Mice ; monoclonal antibody ; Pharmacology. Drug treatments ; schizophyllan ; Sizofiran - analysis ; Sizofiran - blood ; Sizofiran - immunology ; Spectrophotometry, Ultraviolet</subject><ispartof>Biological and Pharmaceutical Bulletin, 1993/11/15, Vol.16(11), pp.1091-1093</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>1994 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1993</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,1884,4025,27928,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3988359$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8312861$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HIRATA, Akio</creatorcontrib><creatorcontrib>ITOH, Wataru</creatorcontrib><creatorcontrib>KOMODA, Makiko</creatorcontrib><creatorcontrib>TABATA, Kengo</creatorcontrib><creatorcontrib>ITOYAMA, Shinji</creatorcontrib><creatorcontrib>SUGAWARA, Isamu</creatorcontrib><title>Detection of Immunoreactive Schizophyllan by Solid-Phase Enzyme-Linked Immunosorbent Assay</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>In order to detect immunoreactive schizophyllan (SPG), one of the β-1, 3-glucans in solution, a murine antischizophyllan monoclonal antibody, SPG1-HS, was used as a primary antibody for solid-phase enzyme-linked immunosorbent assay (ELISA). Polysaccharide-bovine serum albumin (BSA) and polysaccharide-bovine hemoglobin (Hb) conjugate were prepared as an antigen. The absorbance at 490 nm in ELISA was directly proportional to the SPG concentration in SPG-BSA or SPG-Hb conjugates, allowing the accurate measurement of SPG at concentrations of more than 1.0 μg/ml. Also, this ELISA detected SPG in SPG-BSA and SPG-Hb conjugates in human serum in a similar manner. These results suggest that this ELISA is applicable for the determination of SPG concentration in SPG-BSA and SPG-Hb conjugates in human tissue and blood.</description><subject>Analysis</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>monoclonal antibody</subject><subject>Pharmacology. Drug treatments</subject><subject>schizophyllan</subject><subject>Sizofiran - analysis</subject><subject>Sizofiran - blood</subject><subject>Sizofiran - immunology</subject><subject>Spectrophotometry, Ultraviolet</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkM1v1DAQxS0EKkvhxBkpEogLyuKJHcc-Vv2ASisVqXDhYjnOmM2SxIudVEr_erxs2EMvM9K838wbPULeAl1DweXnel-vQayBKnhGVsB4lZcFlM_JKk1kLqCUL8mrGHeU0ooW7IycSQaFFLAiP69wRDu2fsi8y277fhp8QJMmD5jd22376PfbuevMkNVzdu-7tsm_bU3E7Hp4nHvMN-3wG5tlM_pQ4zBmFzGa-TV54UwX8c3Sz8mPm-vvl1_zzd2X28uLTW5LIcZcKO4KMGCUpBxcbaVwlBpVpGIpd8zVVBpbgaiAIneSN4icNapq0IGt2Dn5eLy7D_7PhHHUfRstHn5GP0VdiQKUUCyB75-AOz-FIf2mgXMFpVC0TNSnI2WDjzGg0_vQ9ibMGqg-5K1T3hqEPuSd6HfLzanusTmxS8BJ_7DoJlrTuWAG28YTxpSUrFQJuzliuziaX3jSTRhb2-HBEpRi_2zhf03-J8BuTdA4sL_y2aBe</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>HIRATA, Akio</creator><creator>ITOH, Wataru</creator><creator>KOMODA, Makiko</creator><creator>TABATA, Kengo</creator><creator>ITOYAMA, Shinji</creator><creator>SUGAWARA, Isamu</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1993</creationdate><title>Detection of Immunoreactive Schizophyllan by Solid-Phase Enzyme-Linked Immunosorbent Assay</title><author>HIRATA, Akio ; ITOH, Wataru ; KOMODA, Makiko ; TABATA, Kengo ; ITOYAMA, Shinji ; SUGAWARA, Isamu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c566t-694f21a1a98041fbc86f00a9200ac04f3fb08ac716710e4f84dee43d97def1c73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>monoclonal antibody</topic><topic>Pharmacology. Drug treatments</topic><topic>schizophyllan</topic><topic>Sizofiran - analysis</topic><topic>Sizofiran - blood</topic><topic>Sizofiran - immunology</topic><topic>Spectrophotometry, Ultraviolet</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HIRATA, Akio</creatorcontrib><creatorcontrib>ITOH, Wataru</creatorcontrib><creatorcontrib>KOMODA, Makiko</creatorcontrib><creatorcontrib>TABATA, Kengo</creatorcontrib><creatorcontrib>ITOYAMA, Shinji</creatorcontrib><creatorcontrib>SUGAWARA, Isamu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HIRATA, Akio</au><au>ITOH, Wataru</au><au>KOMODA, Makiko</au><au>TABATA, Kengo</au><au>ITOYAMA, Shinji</au><au>SUGAWARA, Isamu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Immunoreactive Schizophyllan by Solid-Phase Enzyme-Linked Immunosorbent Assay</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>1993</date><risdate>1993</risdate><volume>16</volume><issue>11</issue><spage>1091</spage><epage>1093</epage><pages>1091-1093</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>In order to detect immunoreactive schizophyllan (SPG), one of the β-1, 3-glucans in solution, a murine antischizophyllan monoclonal antibody, SPG1-HS, was used as a primary antibody for solid-phase enzyme-linked immunosorbent assay (ELISA). Polysaccharide-bovine serum albumin (BSA) and polysaccharide-bovine hemoglobin (Hb) conjugate were prepared as an antigen. The absorbance at 490 nm in ELISA was directly proportional to the SPG concentration in SPG-BSA or SPG-Hb conjugates, allowing the accurate measurement of SPG at concentrations of more than 1.0 μg/ml. Also, this ELISA detected SPG in SPG-BSA and SPG-Hb conjugates in human serum in a similar manner. These results suggest that this ELISA is applicable for the determination of SPG concentration in SPG-BSA and SPG-Hb conjugates in human tissue and blood.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>8312861</pmid><doi>10.1248/bpb.16.1091</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biological and Pharmaceutical Bulletin, 1993/11/15, Vol.16(11), pp.1091-1093 |
| issn | 0918-6158 1347-5215 |
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| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Analysis Animals Antibodies, Monoclonal Biological and medical sciences Enzyme-Linked Immunosorbent Assay General pharmacology Humans Medical sciences Mice monoclonal antibody Pharmacology. Drug treatments schizophyllan Sizofiran - analysis Sizofiran - blood Sizofiran - immunology Spectrophotometry, Ultraviolet |
| title | Detection of Immunoreactive Schizophyllan by Solid-Phase Enzyme-Linked Immunosorbent Assay |
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