False localization of acid phosphatase activity in the nuclear envelope and endoplasmic reticulum of peritoneal macrophages
Acid phosphatase cytochemistry using lead salt methods was performed on rat peritoneal macrophages obtained by the intraperitoneal injection of dextran five days previously. Lead precipitate was present in the nuclear envelope, the rough endoplasmic reticulum, Golgi apparatus and lysosomes in about...
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| Veröffentlicht in: | The Histochemical journal 1985-02, Vol.17 (2), p.235-241 |
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| container_title | The Histochemical journal |
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| creator | HOEFSMIT, E. C. M EESTERMANS, I. L KORN, C VAN DUIJN, P |
| description | Acid phosphatase cytochemistry using lead salt methods was performed on rat peritoneal macrophages obtained by the intraperitoneal injection of dextran five days previously. Lead precipitate was present in the nuclear envelope, the rough endoplasmic reticulum, Golgi apparatus and lysosomes in about 50% of these cells. The formation of reaction product appeared to be substrate-specific and was sensitive to sodium fluoride in all these sites. However, only in the nuclear envelope, the rough endoplasmic reticulum and Golgi apparatus could lead salt precipitation be prevented by (a) omission of the washing procedure following the incubation step, (b) postincubation in a medium containing sodium fluoride, or (c) washing in buffer containing lead salt. It is concluded that precipitation of lead salt does not prove the presence of acid phosphatase activity in these organelles. The formation of precipitate in these sites is probably due to a local matrix effect, facilitated by the persistence of acid phosphatase activity in the lysosomes and a suboptimal trapping efficiency of phosphate ions during the washing procedure which follows in the incubation step. |
| doi_str_mv | 10.1007/BF01003222 |
| format | Article |
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C. M ; EESTERMANS, I. L ; KORN, C ; VAN DUIJN, P</creator><creatorcontrib>HOEFSMIT, E. C. M ; EESTERMANS, I. L ; KORN, C ; VAN DUIJN, P</creatorcontrib><description>Acid phosphatase cytochemistry using lead salt methods was performed on rat peritoneal macrophages obtained by the intraperitoneal injection of dextran five days previously. Lead precipitate was present in the nuclear envelope, the rough endoplasmic reticulum, Golgi apparatus and lysosomes in about 50% of these cells. The formation of reaction product appeared to be substrate-specific and was sensitive to sodium fluoride in all these sites. However, only in the nuclear envelope, the rough endoplasmic reticulum and Golgi apparatus could lead salt precipitation be prevented by (a) omission of the washing procedure following the incubation step, (b) postincubation in a medium containing sodium fluoride, or (c) washing in buffer containing lead salt. It is concluded that precipitation of lead salt does not prove the presence of acid phosphatase activity in these organelles. The formation of precipitate in these sites is probably due to a local matrix effect, facilitated by the persistence of acid phosphatase activity in the lysosomes and a suboptimal trapping efficiency of phosphate ions during the washing procedure which follows in the incubation step.</description><identifier>ISSN: 0018-2214</identifier><identifier>EISSN: 1573-6865</identifier><identifier>DOI: 10.1007/BF01003222</identifier><identifier>PMID: 4019251</identifier><identifier>CODEN: HISJAE</identifier><language>eng</language><publisher>London: Kluwer</publisher><subject>acid phosphatase ; Acid Phosphatase - metabolism ; Animals ; Biological and medical sciences ; endoplasmic reticulum ; Endoplasmic Reticulum - enzymology ; False Positive Reactions ; Fundamental and applied biological sciences. Psychology ; Histocytochemistry ; Intestine. Mesentery ; macrophages ; Macrophages - enzymology ; Macrophages - ultrastructure ; Male ; Nuclear Envelope - enzymology ; nuclear envelopes ; Peritoneal Cavity - cytology ; Rats ; Rats, Inbred Strains ; Vertebrates: digestive system</subject><ispartof>The Histochemical journal, 1985-02, Vol.17 (2), p.235-241</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-6984a2e7b9ea6b40308f27282b8a7ce6da37f5e7822d6467a97cdce0fd370eab3</citedby><cites>FETCH-LOGICAL-c342t-6984a2e7b9ea6b40308f27282b8a7ce6da37f5e7822d6467a97cdce0fd370eab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9166941$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4019251$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HOEFSMIT, E. C. M</creatorcontrib><creatorcontrib>EESTERMANS, I. L</creatorcontrib><creatorcontrib>KORN, C</creatorcontrib><creatorcontrib>VAN DUIJN, P</creatorcontrib><title>False localization of acid phosphatase activity in the nuclear envelope and endoplasmic reticulum of peritoneal macrophages</title><title>The Histochemical journal</title><addtitle>Histochem J</addtitle><description>Acid phosphatase cytochemistry using lead salt methods was performed on rat peritoneal macrophages obtained by the intraperitoneal injection of dextran five days previously. Lead precipitate was present in the nuclear envelope, the rough endoplasmic reticulum, Golgi apparatus and lysosomes in about 50% of these cells. The formation of reaction product appeared to be substrate-specific and was sensitive to sodium fluoride in all these sites. However, only in the nuclear envelope, the rough endoplasmic reticulum and Golgi apparatus could lead salt precipitation be prevented by (a) omission of the washing procedure following the incubation step, (b) postincubation in a medium containing sodium fluoride, or (c) washing in buffer containing lead salt. It is concluded that precipitation of lead salt does not prove the presence of acid phosphatase activity in these organelles. The formation of precipitate in these sites is probably due to a local matrix effect, facilitated by the persistence of acid phosphatase activity in the lysosomes and a suboptimal trapping efficiency of phosphate ions during the washing procedure which follows in the incubation step.</description><subject>acid phosphatase</subject><subject>Acid Phosphatase - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>endoplasmic reticulum</subject><subject>Endoplasmic Reticulum - enzymology</subject><subject>False Positive Reactions</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Histocytochemistry</subject><subject>Intestine. Mesentery</subject><subject>macrophages</subject><subject>Macrophages - enzymology</subject><subject>Macrophages - ultrastructure</subject><subject>Male</subject><subject>Nuclear Envelope - enzymology</subject><subject>nuclear envelopes</subject><subject>Peritoneal Cavity - cytology</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Vertebrates: digestive system</subject><issn>0018-2214</issn><issn>1573-6865</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1P3DAQxa2Kii60l96RfEA9IIX6a-3kCIilSEhc6DmaOJOukROntrMS7T9fr1jBsaen0fz05uMR8pWzS86Y-X69YUWlEOIDWfG1kZWu9fqIrBjjdSUEV5_ISUrPjLHGGH1MjhXjjVjzFfm7AZ-Q-mDBuz-QXZhoGChY19N5G9K8hQwFAJvdzuUX6iaat0inxXqESHHaoQ9zAaa-FH2YPaTRWRoxO7v4ZdzbzRhdDhOCpyPYGIrrL0yfycdhP_3LQU_Jz83t082P6uHx7v7m6qGyUolc6aZWINB0DYLuFJOsHoQRtehqMBZ1D9IMazS1EL1W2kBjbG-RDb00DKGTp-Tbq-8cw-8FU25Hlyx6DxOGJbVGC651edv_QK5EI6SqC3jxCpZbUoo4tHN0I8SXlrN2H0n7HkmBzw6uSzdi_4YeMij980MfUklhiDBZl96wpuzWKC7_AeJ7lPw</recordid><startdate>198502</startdate><enddate>198502</enddate><creator>HOEFSMIT, E. C. M</creator><creator>EESTERMANS, I. L</creator><creator>KORN, C</creator><creator>VAN DUIJN, P</creator><general>Kluwer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>198502</creationdate><title>False localization of acid phosphatase activity in the nuclear envelope and endoplasmic reticulum of peritoneal macrophages</title><author>HOEFSMIT, E. C. M ; EESTERMANS, I. L ; KORN, C ; VAN DUIJN, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-6984a2e7b9ea6b40308f27282b8a7ce6da37f5e7822d6467a97cdce0fd370eab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>acid phosphatase</topic><topic>Acid Phosphatase - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>endoplasmic reticulum</topic><topic>Endoplasmic Reticulum - enzymology</topic><topic>False Positive Reactions</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Histocytochemistry</topic><topic>Intestine. Mesentery</topic><topic>macrophages</topic><topic>Macrophages - enzymology</topic><topic>Macrophages - ultrastructure</topic><topic>Male</topic><topic>Nuclear Envelope - enzymology</topic><topic>nuclear envelopes</topic><topic>Peritoneal Cavity - cytology</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HOEFSMIT, E. C. M</creatorcontrib><creatorcontrib>EESTERMANS, I. L</creatorcontrib><creatorcontrib>KORN, C</creatorcontrib><creatorcontrib>VAN DUIJN, P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Histochemical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HOEFSMIT, E. C. M</au><au>EESTERMANS, I. L</au><au>KORN, C</au><au>VAN DUIJN, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>False localization of acid phosphatase activity in the nuclear envelope and endoplasmic reticulum of peritoneal macrophages</atitle><jtitle>The Histochemical journal</jtitle><addtitle>Histochem J</addtitle><date>1985-02</date><risdate>1985</risdate><volume>17</volume><issue>2</issue><spage>235</spage><epage>241</epage><pages>235-241</pages><issn>0018-2214</issn><eissn>1573-6865</eissn><coden>HISJAE</coden><abstract>Acid phosphatase cytochemistry using lead salt methods was performed on rat peritoneal macrophages obtained by the intraperitoneal injection of dextran five days previously. Lead precipitate was present in the nuclear envelope, the rough endoplasmic reticulum, Golgi apparatus and lysosomes in about 50% of these cells. The formation of reaction product appeared to be substrate-specific and was sensitive to sodium fluoride in all these sites. However, only in the nuclear envelope, the rough endoplasmic reticulum and Golgi apparatus could lead salt precipitation be prevented by (a) omission of the washing procedure following the incubation step, (b) postincubation in a medium containing sodium fluoride, or (c) washing in buffer containing lead salt. It is concluded that precipitation of lead salt does not prove the presence of acid phosphatase activity in these organelles. The formation of precipitate in these sites is probably due to a local matrix effect, facilitated by the persistence of acid phosphatase activity in the lysosomes and a suboptimal trapping efficiency of phosphate ions during the washing procedure which follows in the incubation step.</abstract><cop>London</cop><pub>Kluwer</pub><pmid>4019251</pmid><doi>10.1007/BF01003222</doi><tpages>7</tpages></addata></record> |
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| ispartof | The Histochemical journal, 1985-02, Vol.17 (2), p.235-241 |
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| subjects | acid phosphatase Acid Phosphatase - metabolism Animals Biological and medical sciences endoplasmic reticulum Endoplasmic Reticulum - enzymology False Positive Reactions Fundamental and applied biological sciences. Psychology Histocytochemistry Intestine. Mesentery macrophages Macrophages - enzymology Macrophages - ultrastructure Male Nuclear Envelope - enzymology nuclear envelopes Peritoneal Cavity - cytology Rats Rats, Inbred Strains Vertebrates: digestive system |
| title | False localization of acid phosphatase activity in the nuclear envelope and endoplasmic reticulum of peritoneal macrophages |
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