Amphibian lymphokines: 2 Migration inhibition factor produced by antigenic and mitogenic stimulation of amphibian leucocytes
Migration of Rana temporaria peritoneal exudate cells (PEC) was examined in vitro using both direct and indirect assay systems. After sensitization in vivo followed by in vitro challenge 7–21 days later with the appropriate sensitizing antigen, spleen cell culture supernatants were obtained which in...
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| Veröffentlicht in: | Developmental and comparative immunology 1985, Vol.9 (2), p.291-300 |
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| container_title | Developmental and comparative immunology |
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| creator | Gearing, Andrew Rimmer, J.J. |
| description | Migration of
Rana
temporaria
peritoneal exudate cells (PEC) was examined
in
vitro
using both direct and indirect assay systems. After sensitization
in
vivo
followed by
in
vitro
challenge 7–21 days later with the appropriate sensitizing antigen, spleen cell culture supernatants were obtained which inhibited the normal
in
vitro
migration of PEC from non-sensitized animals. Cultures of spleen cells with mitogen also gave rise to supernatants with migration inhibitory properties. Sephadex separation of supernatants showed that maximum inhibitory activity was present in the 27–50,000 MW range and, furthermore, that this inhibition was blocked by α-L-fucose, but not by B-D-galactose. The inhibition did not appear to be species specific. The results indicate that following appropriate stimulation amphibian leucocytes produce a soluble, migration inhibition factor (MIF) with characteristics similar to those described for the mammalian lymphokine MIF. |
| doi_str_mv | 10.1016/0145-305X(85)90120-X |
| format | Article |
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Rana
temporaria
peritoneal exudate cells (PEC) was examined
in
vitro
using both direct and indirect assay systems. After sensitization
in
vivo
followed by
in
vitro
challenge 7–21 days later with the appropriate sensitizing antigen, spleen cell culture supernatants were obtained which inhibited the normal
in
vitro
migration of PEC from non-sensitized animals. Cultures of spleen cells with mitogen also gave rise to supernatants with migration inhibitory properties. Sephadex separation of supernatants showed that maximum inhibitory activity was present in the 27–50,000 MW range and, furthermore, that this inhibition was blocked by α-L-fucose, but not by B-D-galactose. The inhibition did not appear to be species specific. The results indicate that following appropriate stimulation amphibian leucocytes produce a soluble, migration inhibition factor (MIF) with characteristics similar to those described for the mammalian lymphokine MIF.</description><identifier>ISSN: 0145-305X</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/0145-305X(85)90120-X</identifier><identifier>PMID: 2410307</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Animals ; Antigens - immunology ; Ascitic Fluid - immunology ; Concanavalin A - pharmacology ; Erythrocytes - immunology ; gamma-Globulins - immunology ; In Vitro Techniques ; Leukocyte Migration-Inhibitory Factors - biosynthesis ; Leukocytes - immunology ; Lymphokines - biosynthesis ; Mammals - immunology ; Rana temporaria - immunology ; Serum Albumin - immunology ; Species Specificity ; Spleen - immunology</subject><ispartof>Developmental and comparative immunology, 1985, Vol.9 (2), p.291-300</ispartof><rights>1985</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-cb7f126c0a1719a0b1c50e8535635be843b2a6df750c25ff47245d5a7ba60a1d3</citedby><cites>FETCH-LOGICAL-c272t-cb7f126c0a1719a0b1c50e8535635be843b2a6df750c25ff47245d5a7ba60a1d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0145305X8590120X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2410307$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gearing, Andrew</creatorcontrib><creatorcontrib>Rimmer, J.J.</creatorcontrib><title>Amphibian lymphokines: 2 Migration inhibition factor produced by antigenic and mitogenic stimulation of amphibian leucocytes</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>Migration of
Rana
temporaria
peritoneal exudate cells (PEC) was examined
in
vitro
using both direct and indirect assay systems. After sensitization
in
vivo
followed by
in
vitro
challenge 7–21 days later with the appropriate sensitizing antigen, spleen cell culture supernatants were obtained which inhibited the normal
in
vitro
migration of PEC from non-sensitized animals. Cultures of spleen cells with mitogen also gave rise to supernatants with migration inhibitory properties. Sephadex separation of supernatants showed that maximum inhibitory activity was present in the 27–50,000 MW range and, furthermore, that this inhibition was blocked by α-L-fucose, but not by B-D-galactose. The inhibition did not appear to be species specific. The results indicate that following appropriate stimulation amphibian leucocytes produce a soluble, migration inhibition factor (MIF) with characteristics similar to those described for the mammalian lymphokine MIF.</description><subject>Animals</subject><subject>Antigens - immunology</subject><subject>Ascitic Fluid - immunology</subject><subject>Concanavalin A - pharmacology</subject><subject>Erythrocytes - immunology</subject><subject>gamma-Globulins - immunology</subject><subject>In Vitro Techniques</subject><subject>Leukocyte Migration-Inhibitory Factors - biosynthesis</subject><subject>Leukocytes - immunology</subject><subject>Lymphokines - biosynthesis</subject><subject>Mammals - immunology</subject><subject>Rana temporaria - immunology</subject><subject>Serum Albumin - immunology</subject><subject>Species Specificity</subject><subject>Spleen - immunology</subject><issn>0145-305X</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9P3DAQxS0EogvtNwDJJwSHlLETxwkHJIT6T1rEpZX2ZjnOBNwm8dZ2Kq3Eh8e7WcGtvnhG894b-0fIGYPPDFh5DawQWQ5idVmJqxoYh2x1QBasknUGUNWHZPEm-UBOQvgN6VQMjskxLxjkIBfk5W5YP9vG6pH2m1S6P3bEcEM5fbBPXkfrRmrHrWJXdtpE5-nau3Yy2NJmQ_UY7ROO1qSqpYONbu5CtMPUzwmuo_p9D07GmU3E8JEcdboP-Gl_n5JfX7_8vP-eLR-__bi_W2aGSx4z08iO8dKAZpLVGhpmBGAlclHmosGqyBuuy7aTAgwXXVdIXohWaNnoMnna_JRczLnp3X8nDFENNhjsez2im4KSJWdCsDoJi1lovAvBY6fW3g7abxQDtYWutkTVlqiqhNpBV6tkO9_nT82A7ZtpTznNb-c5pk_-s-hVMBbHBNB6NFG1zv5_wSu9lZPe</recordid><startdate>1985</startdate><enddate>1985</enddate><creator>Gearing, Andrew</creator><creator>Rimmer, J.J.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1985</creationdate><title>Amphibian lymphokines: 2 Migration inhibition factor produced by antigenic and mitogenic stimulation of amphibian leucocytes</title><author>Gearing, Andrew ; Rimmer, J.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-cb7f126c0a1719a0b1c50e8535635be843b2a6df750c25ff47245d5a7ba60a1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Antigens - immunology</topic><topic>Ascitic Fluid - immunology</topic><topic>Concanavalin A - pharmacology</topic><topic>Erythrocytes - immunology</topic><topic>gamma-Globulins - immunology</topic><topic>In Vitro Techniques</topic><topic>Leukocyte Migration-Inhibitory Factors - biosynthesis</topic><topic>Leukocytes - immunology</topic><topic>Lymphokines - biosynthesis</topic><topic>Mammals - immunology</topic><topic>Rana temporaria - immunology</topic><topic>Serum Albumin - immunology</topic><topic>Species Specificity</topic><topic>Spleen - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gearing, Andrew</creatorcontrib><creatorcontrib>Rimmer, J.J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gearing, Andrew</au><au>Rimmer, J.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amphibian lymphokines: 2 Migration inhibition factor produced by antigenic and mitogenic stimulation of amphibian leucocytes</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>1985</date><risdate>1985</risdate><volume>9</volume><issue>2</issue><spage>291</spage><epage>300</epage><pages>291-300</pages><issn>0145-305X</issn><eissn>1879-0089</eissn><abstract>Migration of
Rana
temporaria
peritoneal exudate cells (PEC) was examined
in
vitro
using both direct and indirect assay systems. After sensitization
in
vivo
followed by
in
vitro
challenge 7–21 days later with the appropriate sensitizing antigen, spleen cell culture supernatants were obtained which inhibited the normal
in
vitro
migration of PEC from non-sensitized animals. Cultures of spleen cells with mitogen also gave rise to supernatants with migration inhibitory properties. Sephadex separation of supernatants showed that maximum inhibitory activity was present in the 27–50,000 MW range and, furthermore, that this inhibition was blocked by α-L-fucose, but not by B-D-galactose. The inhibition did not appear to be species specific. The results indicate that following appropriate stimulation amphibian leucocytes produce a soluble, migration inhibition factor (MIF) with characteristics similar to those described for the mammalian lymphokine MIF.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>2410307</pmid><doi>10.1016/0145-305X(85)90120-X</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0145-305X |
| ispartof | Developmental and comparative immunology, 1985, Vol.9 (2), p.291-300 |
| issn | 0145-305X 1879-0089 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76215519 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antigens - immunology Ascitic Fluid - immunology Concanavalin A - pharmacology Erythrocytes - immunology gamma-Globulins - immunology In Vitro Techniques Leukocyte Migration-Inhibitory Factors - biosynthesis Leukocytes - immunology Lymphokines - biosynthesis Mammals - immunology Rana temporaria - immunology Serum Albumin - immunology Species Specificity Spleen - immunology |
| title | Amphibian lymphokines: 2 Migration inhibition factor produced by antigenic and mitogenic stimulation of amphibian leucocytes |
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