Footprinting of DNA secondary structure by high-intensity (laser) ultraviolet irradiation
The action of high-intensity ultraviolet pulse laser radiation on a 161 bp fragment of pBR 322 DNA ( EcoRI- Msp I fragment) was studied. At doses up to 5 × 10 18 photons/cm 2 the N-glycosidic bond splitting is negligible. The action of hot piperidine on irradiated DNA leads to chain splitting at the...
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| Veröffentlicht in: | FEBS letters 1985-08, Vol.188 (1), p.155-158 |
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| creator | Budowsky, E.I. Kovalsky, O.I. Yakovlev, D.Yu Simukova, N.A. Rubin, L.B. |
| description | The action of high-intensity ultraviolet pulse laser radiation on a 161 bp fragment of pBR 322 DNA (
EcoRI-
Msp I fragment) was studied. At doses up to 5 × 10
18 photons/cm
2 the
N-glycosidic bond splitting is negligible. The action of hot piperidine on irradiated DNA leads to chain splitting at the residues, modified via biphotonic processes. The modification and, hence, splitting efficiences depend on the type of base (G>T>A>C) and on its position in the sequence. Preferentially modified bases in the opposite strands of double-stranded DNA belong, mainly, to the same or adjacent base pairs. Residues in the Pribnow box are modified considerably less, than in the sequences, immediately upstream and downstream. This approach seems to be useful in footprinting of DNA secondary structure peculiarities and alterations, conjugated with the functional role and state of the respective fragment. |
| doi_str_mv | 10.1016/0014-5793(85)80894-2 |
| format | Article |
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EcoRI-
Msp I fragment) was studied. At doses up to 5 × 10
18 photons/cm
2 the
N-glycosidic bond splitting is negligible. The action of hot piperidine on irradiated DNA leads to chain splitting at the residues, modified via biphotonic processes. The modification and, hence, splitting efficiences depend on the type of base (G>T>A>C) and on its position in the sequence. Preferentially modified bases in the opposite strands of double-stranded DNA belong, mainly, to the same or adjacent base pairs. Residues in the Pribnow box are modified considerably less, than in the sequences, immediately upstream and downstream. This approach seems to be useful in footprinting of DNA secondary structure peculiarities and alterations, conjugated with the functional role and state of the respective fragment.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(85)80894-2</identifier><identifier>PMID: 4018270</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Base Sequence ; Biological and medical sciences ; DNA - metabolism ; DNA - radiation effects ; DNA secondary structure ; DNA splitting ; Endodeoxyribonucleases - metabolism ; Escherichia coli ; Footprinting ; Fundamental and applied biological sciences. Psychology ; Glycosides - metabolism ; Laser ultraviolet irradiation ; Lasers ; Molecular biophysics ; Nucleic Acid Conformation ; Photochemistry. Photosynthesis. Bioluminescence ; Piperidines - pharmacology ; Plasmid pBR 322 ; Plasmids ; Pyrimidine Dimers - metabolism ; Radiation-biomolecule interaction ; Ultraviolet Rays</subject><ispartof>FEBS letters, 1985-08, Vol.188 (1), p.155-158</ispartof><rights>1985</rights><rights>FEBS Letters 188 (1985) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5462-e12c62dabf675bd13d0d543934f217d25a796c510f6f1f73b38943f0ef2c9cb3</citedby><cites>FETCH-LOGICAL-c5462-e12c62dabf675bd13d0d543934f217d25a796c510f6f1f73b38943f0ef2c9cb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579385808942$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8615617$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4018270$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Budowsky, E.I.</creatorcontrib><creatorcontrib>Kovalsky, O.I.</creatorcontrib><creatorcontrib>Yakovlev, D.Yu</creatorcontrib><creatorcontrib>Simukova, N.A.</creatorcontrib><creatorcontrib>Rubin, L.B.</creatorcontrib><title>Footprinting of DNA secondary structure by high-intensity (laser) ultraviolet irradiation</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The action of high-intensity ultraviolet pulse laser radiation on a 161 bp fragment of pBR 322 DNA (
EcoRI-
Msp I fragment) was studied. At doses up to 5 × 10
18 photons/cm
2 the
N-glycosidic bond splitting is negligible. The action of hot piperidine on irradiated DNA leads to chain splitting at the residues, modified via biphotonic processes. The modification and, hence, splitting efficiences depend on the type of base (G>T>A>C) and on its position in the sequence. Preferentially modified bases in the opposite strands of double-stranded DNA belong, mainly, to the same or adjacent base pairs. Residues in the Pribnow box are modified considerably less, than in the sequences, immediately upstream and downstream. This approach seems to be useful in footprinting of DNA secondary structure peculiarities and alterations, conjugated with the functional role and state of the respective fragment.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA - metabolism</subject><subject>DNA - radiation effects</subject><subject>DNA secondary structure</subject><subject>DNA splitting</subject><subject>Endodeoxyribonucleases - metabolism</subject><subject>Escherichia coli</subject><subject>Footprinting</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycosides - metabolism</subject><subject>Laser ultraviolet irradiation</subject><subject>Lasers</subject><subject>Molecular biophysics</subject><subject>Nucleic Acid Conformation</subject><subject>Photochemistry. Photosynthesis. Bioluminescence</subject><subject>Piperidines - pharmacology</subject><subject>Plasmid pBR 322</subject><subject>Plasmids</subject><subject>Pyrimidine Dimers - metabolism</subject><subject>Radiation-biomolecule interaction</subject><subject>Ultraviolet Rays</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE9vFCEYh4nR1G31G2gyB2Pawyj_h7k0qW1XTRq99OKJMPDSYmaHCkzNfnuZ7maP6gl434cfvA9Cbwj-QDCRHzEmvBVdz06VOFNY9bylz9CKqI61jEv1HK0OyEt0nPNPXM-K9EfoiNcN7fAK_VjHWB5SmEqY7prom6tvF00GGydn0rbJJc22zAmaYdvch7v7tpIw5VC2zeloMqSzZh5LMo8hjlCakJJxwZQQp1fohTdjhtf79QTdrq9vL7-0N98_f728uGmt4JK2QKiV1JnBy04MjjCHneCsZ9xT0jkqTNdLKwj20hPfsYHVQZnH4Knt7cBO0Ptd7EOKv2bIRW9CtjCOZoI4Z91JSmgv6T9BwimRTPEK8h1oU8w5gdfVz6ba0ATrxbxetOpFq1ZCP5nXS_7bff48bMAdLu1V1_67fd9ka0afzGRDPmBKEiFJV7H1DvsdRtj-19N6ff2JLo2lrsRTdfnP-S4IqvzHAElnG2Cy4EICW7SL4e8D_QHyprKx</recordid><startdate>19850819</startdate><enddate>19850819</enddate><creator>Budowsky, E.I.</creator><creator>Kovalsky, O.I.</creator><creator>Yakovlev, D.Yu</creator><creator>Simukova, N.A.</creator><creator>Rubin, L.B.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19850819</creationdate><title>Footprinting of DNA secondary structure by high-intensity (laser) ultraviolet irradiation</title><author>Budowsky, E.I. ; Kovalsky, O.I. ; Yakovlev, D.Yu ; Simukova, N.A. ; Rubin, L.B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5462-e12c62dabf675bd13d0d543934f217d25a796c510f6f1f73b38943f0ef2c9cb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA - metabolism</topic><topic>DNA - radiation effects</topic><topic>DNA secondary structure</topic><topic>DNA splitting</topic><topic>Endodeoxyribonucleases - metabolism</topic><topic>Escherichia coli</topic><topic>Footprinting</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycosides - metabolism</topic><topic>Laser ultraviolet irradiation</topic><topic>Lasers</topic><topic>Molecular biophysics</topic><topic>Nucleic Acid Conformation</topic><topic>Photochemistry. Photosynthesis. Bioluminescence</topic><topic>Piperidines - pharmacology</topic><topic>Plasmid pBR 322</topic><topic>Plasmids</topic><topic>Pyrimidine Dimers - metabolism</topic><topic>Radiation-biomolecule interaction</topic><topic>Ultraviolet Rays</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Budowsky, E.I.</creatorcontrib><creatorcontrib>Kovalsky, O.I.</creatorcontrib><creatorcontrib>Yakovlev, D.Yu</creatorcontrib><creatorcontrib>Simukova, N.A.</creatorcontrib><creatorcontrib>Rubin, L.B.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Budowsky, E.I.</au><au>Kovalsky, O.I.</au><au>Yakovlev, D.Yu</au><au>Simukova, N.A.</au><au>Rubin, L.B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Footprinting of DNA secondary structure by high-intensity (laser) ultraviolet irradiation</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1985-08-19</date><risdate>1985</risdate><volume>188</volume><issue>1</issue><spage>155</spage><epage>158</epage><pages>155-158</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>The action of high-intensity ultraviolet pulse laser radiation on a 161 bp fragment of pBR 322 DNA (
EcoRI-
Msp I fragment) was studied. At doses up to 5 × 10
18 photons/cm
2 the
N-glycosidic bond splitting is negligible. The action of hot piperidine on irradiated DNA leads to chain splitting at the residues, modified via biphotonic processes. The modification and, hence, splitting efficiences depend on the type of base (G>T>A>C) and on its position in the sequence. Preferentially modified bases in the opposite strands of double-stranded DNA belong, mainly, to the same or adjacent base pairs. Residues in the Pribnow box are modified considerably less, than in the sequences, immediately upstream and downstream. This approach seems to be useful in footprinting of DNA secondary structure peculiarities and alterations, conjugated with the functional role and state of the respective fragment.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>4018270</pmid><doi>10.1016/0014-5793(85)80894-2</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
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| ispartof | FEBS letters, 1985-08, Vol.188 (1), p.155-158 |
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| source | MEDLINE; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Base Sequence Biological and medical sciences DNA - metabolism DNA - radiation effects DNA secondary structure DNA splitting Endodeoxyribonucleases - metabolism Escherichia coli Footprinting Fundamental and applied biological sciences. Psychology Glycosides - metabolism Laser ultraviolet irradiation Lasers Molecular biophysics Nucleic Acid Conformation Photochemistry. Photosynthesis. Bioluminescence Piperidines - pharmacology Plasmid pBR 322 Plasmids Pyrimidine Dimers - metabolism Radiation-biomolecule interaction Ultraviolet Rays |
| title | Footprinting of DNA secondary structure by high-intensity (laser) ultraviolet irradiation |
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