Cloning, sequencing and phylogenetic analysis of a human 5-hydroxytryptamine 1D receptor pseudogene
A third member of the human 5 HT ID gene family has been identified using a combination of homology cloning and DNA sequence analysis. This human gene is most related to the 5 HT 1 Dα subtype (77% shared identity) and is a pseudogene, based on the lack of an open reading frame (ORF) caused by multip...
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| Veröffentlicht in: | Gene 1993-12, Vol.137 (2), p.339-344 |
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| creator | Shuck, Mary E. Veldman, Sarah A. Bienkowski, Michael J. |
| description | A third member of the human 5
HT
ID gene family has been identified using a combination of homology cloning and DNA sequence analysis. This human gene is most related to the 5
HT
1
Dα
subtype (77% shared identity) and is a pseudogene, based on the lack of an open reading frame (ORF) caused by multiple in-frame stop codons and nucleotide (nt) deletions relative to the functional 5
HT
1
Dα
gene (encoding the 5-hydroxytryptamine
1Dα receptor). The 5
HT
1
D
pseudogene also contained an insertion that shares 87% identity to the
Alu consensus sequence. Phylogenetic analysis of the three human genes in this family reveals that although the two functional genes, 5
HT
1
Dα
and 5
HT
1
Dgb
, are detected in all mammalian species examined, the 5
HT
1
D
pseudogene is only detected in a subset of primates (catarrhines) that evolved approximately 35–45 million years (Myr) ago. Alternatively, based on the 23% divergence between the functional 5
HT
1
Dα
gene and the 5
HT
1
D
pseudogene, we estimate that these two genes began to diverge approximately 50 Myr ago. |
| doi_str_mv | 10.1016/0378-1119(93)90031-W |
| format | Article |
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HT
ID gene family has been identified using a combination of homology cloning and DNA sequence analysis. This human gene is most related to the 5
HT
1
Dα
subtype (77% shared identity) and is a pseudogene, based on the lack of an open reading frame (ORF) caused by multiple in-frame stop codons and nucleotide (nt) deletions relative to the functional 5
HT
1
Dα
gene (encoding the 5-hydroxytryptamine
1Dα receptor). The 5
HT
1
D
pseudogene also contained an insertion that shares 87% identity to the
Alu consensus sequence. Phylogenetic analysis of the three human genes in this family reveals that although the two functional genes, 5
HT
1
Dα
and 5
HT
1
Dgb
, are detected in all mammalian species examined, the 5
HT
1
D
pseudogene is only detected in a subset of primates (catarrhines) that evolved approximately 35–45 million years (Myr) ago. Alternatively, based on the 23% divergence between the functional 5
HT
1
Dα
gene and the 5
HT
1
D
pseudogene, we estimate that these two genes began to diverge approximately 50 Myr ago.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(93)90031-W</identifier><identifier>PMID: 8299968</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Alu repeat ; Amino Acid Sequence ; Base Sequence ; Cloning, Molecular ; DNA ; evolution ; Humans ; Molecular Sequence Data ; Multigene Family ; Phylogeny ; primate DNA ; Pseudogenes ; Receptors, Serotonin - genetics ; serotonin receptor</subject><ispartof>Gene, 1993-12, Vol.137 (2), p.339-344</ispartof><rights>1993</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/037811199390031W$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8299968$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shuck, Mary E.</creatorcontrib><creatorcontrib>Veldman, Sarah A.</creatorcontrib><creatorcontrib>Bienkowski, Michael J.</creatorcontrib><title>Cloning, sequencing and phylogenetic analysis of a human 5-hydroxytryptamine 1D receptor pseudogene</title><title>Gene</title><addtitle>Gene</addtitle><description>A third member of the human 5
HT
ID gene family has been identified using a combination of homology cloning and DNA sequence analysis. This human gene is most related to the 5
HT
1
Dα
subtype (77% shared identity) and is a pseudogene, based on the lack of an open reading frame (ORF) caused by multiple in-frame stop codons and nucleotide (nt) deletions relative to the functional 5
HT
1
Dα
gene (encoding the 5-hydroxytryptamine
1Dα receptor). The 5
HT
1
D
pseudogene also contained an insertion that shares 87% identity to the
Alu consensus sequence. Phylogenetic analysis of the three human genes in this family reveals that although the two functional genes, 5
HT
1
Dα
and 5
HT
1
Dgb
, are detected in all mammalian species examined, the 5
HT
1
D
pseudogene is only detected in a subset of primates (catarrhines) that evolved approximately 35–45 million years (Myr) ago. Alternatively, based on the 23% divergence between the functional 5
HT
1
Dα
gene and the 5
HT
1
D
pseudogene, we estimate that these two genes began to diverge approximately 50 Myr ago.</description><subject>Alu repeat</subject><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>evolution</subject><subject>Humans</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Phylogeny</subject><subject>primate DNA</subject><subject>Pseudogenes</subject><subject>Receptors, Serotonin - genetics</subject><subject>serotonin receptor</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UclKBDEQDaKM4-gfKOQkCrZm6SW5CDKuMOBFmWNI0tUzkd7sdIv992YWrEtVvXoU9V4hdE7JLSU0vSM8ExGlVF5Jfi0J4TRaHqApFZmMQicO0fSfcoxOvP8iIZKETdBEMCllKqbIzsumdvXqBnv4HqC2oca6znG7HstmBTX0zgZAl6N3HjcF1ng9VLrGSbQe8675HftubHtduRowfcQdWGj7psOthyHfbjhFR4UuPZzt8wx9Pj99zF-jxfvL2_xhEQElQkTcJKwwsc0giTNdUEmM5Vmsdca1McQwAUYQK41msTEyCy1jIiA0YaBJzGfocre37Zqgxfeqct5CWeoamsGrLGVEpIwF4sWeOJgKctV2rtLdqPauhPn9bg7h2h8HnfLWBW8gd0Fer_LGKUrU5gtqY7HaWKwkV9svqCX_Aw4mei4</recordid><startdate>19931231</startdate><enddate>19931231</enddate><creator>Shuck, Mary E.</creator><creator>Veldman, Sarah A.</creator><creator>Bienkowski, Michael J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19931231</creationdate><title>Cloning, sequencing and phylogenetic analysis of a human 5-hydroxytryptamine 1D receptor pseudogene</title><author>Shuck, Mary E. ; Veldman, Sarah A. ; Bienkowski, Michael J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e1088-3b52fb4c7e547af190bc374aa73abb0b28eb80c9ba24bb978eb22880c152ea043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Alu repeat</topic><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>DNA</topic><topic>evolution</topic><topic>Humans</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Phylogeny</topic><topic>primate DNA</topic><topic>Pseudogenes</topic><topic>Receptors, Serotonin - genetics</topic><topic>serotonin receptor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shuck, Mary E.</creatorcontrib><creatorcontrib>Veldman, Sarah A.</creatorcontrib><creatorcontrib>Bienkowski, Michael J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shuck, Mary E.</au><au>Veldman, Sarah A.</au><au>Bienkowski, Michael J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning, sequencing and phylogenetic analysis of a human 5-hydroxytryptamine 1D receptor pseudogene</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1993-12-31</date><risdate>1993</risdate><volume>137</volume><issue>2</issue><spage>339</spage><epage>344</epage><pages>339-344</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>A third member of the human 5
HT
ID gene family has been identified using a combination of homology cloning and DNA sequence analysis. This human gene is most related to the 5
HT
1
Dα
subtype (77% shared identity) and is a pseudogene, based on the lack of an open reading frame (ORF) caused by multiple in-frame stop codons and nucleotide (nt) deletions relative to the functional 5
HT
1
Dα
gene (encoding the 5-hydroxytryptamine
1Dα receptor). The 5
HT
1
D
pseudogene also contained an insertion that shares 87% identity to the
Alu consensus sequence. Phylogenetic analysis of the three human genes in this family reveals that although the two functional genes, 5
HT
1
Dα
and 5
HT
1
Dgb
, are detected in all mammalian species examined, the 5
HT
1
D
pseudogene is only detected in a subset of primates (catarrhines) that evolved approximately 35–45 million years (Myr) ago. Alternatively, based on the 23% divergence between the functional 5
HT
1
Dα
gene and the 5
HT
1
D
pseudogene, we estimate that these two genes began to diverge approximately 50 Myr ago.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>8299968</pmid><doi>10.1016/0378-1119(93)90031-W</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Gene, 1993-12, Vol.137 (2), p.339-344 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76208622 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alu repeat Amino Acid Sequence Base Sequence Cloning, Molecular DNA evolution Humans Molecular Sequence Data Multigene Family Phylogeny primate DNA Pseudogenes Receptors, Serotonin - genetics serotonin receptor |
| title | Cloning, sequencing and phylogenetic analysis of a human 5-hydroxytryptamine 1D receptor pseudogene |
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