Turnover of nascent proteins in HeLa-S3 cells and the quasi-linear incorporation kinetics of amino acids
The turnover of nascent proteins in HeLa S-3 cells was estimated simultaneously with synthesis by the difference between the expected accumulation of radioactivity, based on rate determinations from very short pulse treatments, from that observed by continuous exposure for 100 min. The results were...
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| Veröffentlicht in: | Cell biology international reports 1985-05, Vol.9 (5), p.463-470 |
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| container_title | Cell biology international reports |
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| creator | Wheatley, Denys N. Inglis, Marget S. |
| description | The turnover of nascent proteins in HeLa S-3 cells was estimated simultaneously with synthesis by the difference between the expected accumulation of radioactivity, based on rate determinations from very short pulse treatments, from that observed by continuous exposure for 100 min. The results were similar to those from conventional pulse-chase analysis, but not identical for reasons which are discussed. They confirm that extensive protein turnover accompanies synthesis in the growing cell, and therefore net synthesis is much lower than gross synthesis.
Cells retained all proteins synthesised during heat treatment at 45 °C, indicating the complete inhibition of protein degradation, cells retaining all proteins synthesised. This particular perturbation experimentally validated the deficit method, and its potential usefulness in future turnover studies. |
| doi_str_mv | 10.1016/0309-1651(85)90154-7 |
| format | Article |
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Cells retained all proteins synthesised during heat treatment at 45 °C, indicating the complete inhibition of protein degradation, cells retaining all proteins synthesised. This particular perturbation experimentally validated the deficit method, and its potential usefulness in future turnover studies.</description><identifier>ISSN: 0309-1651</identifier><identifier>EISSN: 1878-240X</identifier><identifier>DOI: 10.1016/0309-1651(85)90154-7</identifier><identifier>PMID: 4016964</identifier><identifier>CODEN: CBRPDS</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Amino Acids - metabolism ; Biological and medical sciences ; Cell Division ; Cell metabolism, cell oxidation ; Cell physiology ; Fundamental and applied biological sciences. Psychology ; HeLa Cells - metabolism ; Hot Temperature ; Humans ; Kinetics ; Molecular and cellular biology ; Protein Biosynthesis ; Proteins - metabolism</subject><ispartof>Cell biology international reports, 1985-05, Vol.9 (5), p.463-470</ispartof><rights>1985</rights><rights>1985 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-57efc892c37647b5cba8f4ad15d85843f7c36568f8e718a2bd623e3fd54c52bc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9267259$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4016964$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wheatley, Denys N.</creatorcontrib><creatorcontrib>Inglis, Marget S.</creatorcontrib><title>Turnover of nascent proteins in HeLa-S3 cells and the quasi-linear incorporation kinetics of amino acids</title><title>Cell biology international reports</title><addtitle>Cell Biol Int Rep</addtitle><description>The turnover of nascent proteins in HeLa S-3 cells was estimated simultaneously with synthesis by the difference between the expected accumulation of radioactivity, based on rate determinations from very short pulse treatments, from that observed by continuous exposure for 100 min. The results were similar to those from conventional pulse-chase analysis, but not identical for reasons which are discussed. They confirm that extensive protein turnover accompanies synthesis in the growing cell, and therefore net synthesis is much lower than gross synthesis.
Cells retained all proteins synthesised during heat treatment at 45 °C, indicating the complete inhibition of protein degradation, cells retaining all proteins synthesised. This particular perturbation experimentally validated the deficit method, and its potential usefulness in future turnover studies.</description><subject>Amino Acids - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cell Division</subject><subject>Cell metabolism, cell oxidation</subject><subject>Cell physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HeLa Cells - metabolism</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Kinetics</subject><subject>Molecular and cellular biology</subject><subject>Protein Biosynthesis</subject><subject>Proteins - metabolism</subject><issn>0309-1651</issn><issn>1878-240X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM9rFDEUx4NY6lr9DxRyENHDaDL5OZeCFLWFhR6s4C1kkhcanU22yUyh_70Zd9mjp0fe-7wvLx-E3lDyiRIqPxNGho5KQT9o8XEgVPBOPUMbqpXuek5-PUebE_ICvaz1N2lvJoZzdM5bwCD5Bt3fLSXlRyg4B5xsdZBmvC95hpgqjglfw9Z2Pxh2ME0V2-TxfA_4YbE1dlNMYEujXC77XOwcc8J_WnOOrq6BdhdTxtZFX1-hs2CnCq-P9QL9_Pb17uq6295-v7n6su0c03LuhILg9NA7piRXo3Cj1YFbT4XXQnMWlGNSSB00KKptP3rZM2DBC-5EPzp2gd4fctsnHhaos9nFuh5vE-SlGiV7olp4A_kBdCXXWiCYfYk7W54MJWYVbFZ7ZrVntDD_BBvV1t4e85dxB_60dDTa5u-O8ybTTqHY5GI9YUMvVS-Ghl0eMGguHiMUU12E5MDHAm42Psf_3_EXO8mXjA</recordid><startdate>198505</startdate><enddate>198505</enddate><creator>Wheatley, Denys N.</creator><creator>Inglis, Marget S.</creator><general>Elsevier B.V</general><general>Academic Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198505</creationdate><title>Turnover of nascent proteins in HeLa-S3 cells and the quasi-linear incorporation kinetics of amino acids</title><author>Wheatley, Denys N. ; Inglis, Marget S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-57efc892c37647b5cba8f4ad15d85843f7c36568f8e718a2bd623e3fd54c52bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Amino Acids - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cell Division</topic><topic>Cell metabolism, cell oxidation</topic><topic>Cell physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HeLa Cells - metabolism</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Kinetics</topic><topic>Molecular and cellular biology</topic><topic>Protein Biosynthesis</topic><topic>Proteins - metabolism</topic><toplevel>online_resources</toplevel><creatorcontrib>Wheatley, Denys N.</creatorcontrib><creatorcontrib>Inglis, Marget S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biology international reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wheatley, Denys N.</au><au>Inglis, Marget S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Turnover of nascent proteins in HeLa-S3 cells and the quasi-linear incorporation kinetics of amino acids</atitle><jtitle>Cell biology international reports</jtitle><addtitle>Cell Biol Int Rep</addtitle><date>1985-05</date><risdate>1985</risdate><volume>9</volume><issue>5</issue><spage>463</spage><epage>470</epage><pages>463-470</pages><issn>0309-1651</issn><eissn>1878-240X</eissn><coden>CBRPDS</coden><abstract>The turnover of nascent proteins in HeLa S-3 cells was estimated simultaneously with synthesis by the difference between the expected accumulation of radioactivity, based on rate determinations from very short pulse treatments, from that observed by continuous exposure for 100 min. The results were similar to those from conventional pulse-chase analysis, but not identical for reasons which are discussed. They confirm that extensive protein turnover accompanies synthesis in the growing cell, and therefore net synthesis is much lower than gross synthesis.
Cells retained all proteins synthesised during heat treatment at 45 °C, indicating the complete inhibition of protein degradation, cells retaining all proteins synthesised. This particular perturbation experimentally validated the deficit method, and its potential usefulness in future turnover studies.</abstract><cop>London</cop><cop>San Diego, CA</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>4016964</pmid><doi>10.1016/0309-1651(85)90154-7</doi><tpages>8</tpages></addata></record> |
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| ispartof | Cell biology international reports, 1985-05, Vol.9 (5), p.463-470 |
| issn | 0309-1651 1878-240X |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Amino Acids - metabolism Biological and medical sciences Cell Division Cell metabolism, cell oxidation Cell physiology Fundamental and applied biological sciences. Psychology HeLa Cells - metabolism Hot Temperature Humans Kinetics Molecular and cellular biology Protein Biosynthesis Proteins - metabolism |
| title | Turnover of nascent proteins in HeLa-S3 cells and the quasi-linear incorporation kinetics of amino acids |
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