Nuclear DNA in intestinal carcinoid tumors. A study before and after cytotoxin (streptozotocin and 5‐Fluorouracil) treatment
Imprint cytology specimens of metastases of intestinal carcinoids obtained by percutaneous biopsy were analysed cytofluorometrically with regard to nuclear DNA records. All untreated tumors (nine cases) exhibited diploid DNA values with a relatively low proliferative activity (less than 2% nuclei in...
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| Veröffentlicht in: | Cancer 1985-08, Vol.56 (4), p.793-796 |
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| creator | Wilander, Erik Bengtsson, Allan Lindgren, Per G. Lundqvist, Monalill Norheim, Ingrid Öberg, Kjell |
| description | Imprint cytology specimens of metastases of intestinal carcinoids obtained by percutaneous biopsy were analysed cytofluorometrically with regard to nuclear DNA records. All untreated tumors (nine cases) exhibited diploid DNA values with a relatively low proliferative activity (less than 2% nuclei in Sphase region). The mean number of tetraploid cells was 5%. Cytofluorometry also was performed on five tumor metastases treated with the cytotoxin streptozotocin and 5‐fluorouracil. After treatment, an increase in the number of tetraploid cells (mean value, 30%) was noted, indicating that the cytotoxin treatment (possibly streptozotocin) on the tumor cells in vivo blocked progression from G2 to M phase. The current cytofluorometric analyses show that diploid nuclear DNA records and a low proliferative activity is a characteristic of malignant carcinoid tumors of the intestine. Due to regular DNA histograms in the carcinoid tumors, it is suggested that reliable studies are permitted of the effect of cytotoxins on the different phases of the cell cycle in vivo. |
| doi_str_mv | 10.1002/1097-0142(19850815)56:4<793::AID-CNCR2820560416>3.0.CO;2-W |
| format | Article |
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A study before and after cytotoxin (streptozotocin and 5‐Fluorouracil) treatment</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Wilander, Erik ; Bengtsson, Allan ; Lindgren, Per G. ; Lundqvist, Monalill ; Norheim, Ingrid ; Öberg, Kjell</creator><creatorcontrib>Wilander, Erik ; Bengtsson, Allan ; Lindgren, Per G. ; Lundqvist, Monalill ; Norheim, Ingrid ; Öberg, Kjell</creatorcontrib><description>Imprint cytology specimens of metastases of intestinal carcinoids obtained by percutaneous biopsy were analysed cytofluorometrically with regard to nuclear DNA records. All untreated tumors (nine cases) exhibited diploid DNA values with a relatively low proliferative activity (less than 2% nuclei in Sphase region). The mean number of tetraploid cells was 5%. Cytofluorometry also was performed on five tumor metastases treated with the cytotoxin streptozotocin and 5‐fluorouracil. After treatment, an increase in the number of tetraploid cells (mean value, 30%) was noted, indicating that the cytotoxin treatment (possibly streptozotocin) on the tumor cells in vivo blocked progression from G2 to M phase. The current cytofluorometric analyses show that diploid nuclear DNA records and a low proliferative activity is a characteristic of malignant carcinoid tumors of the intestine. Due to regular DNA histograms in the carcinoid tumors, it is suggested that reliable studies are permitted of the effect of cytotoxins on the different phases of the cell cycle in vivo.</description><identifier>ISSN: 0008-543X</identifier><identifier>EISSN: 1097-0142</identifier><identifier>DOI: 10.1002/1097-0142(19850815)56:4<793::AID-CNCR2820560416>3.0.CO;2-W</identifier><identifier>PMID: 3160455</identifier><identifier>CODEN: CANCAR</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Aged ; Antineoplastic agents ; Antineoplastic Combined Chemotherapy Protocols - therapeutic use ; Biological and medical sciences ; Carcinoid Tumor - drug therapy ; Carcinoid Tumor - metabolism ; Carcinoid Tumor - pathology ; Cell Nucleus - metabolism ; Chemotherapy ; Diploidy ; DNA - genetics ; DNA - metabolism ; Female ; Flow Cytometry ; Fluorouracil - administration & dosage ; Humans ; Intestinal Neoplasms - drug therapy ; Intestinal Neoplasms - metabolism ; Intestinal Neoplasms - pathology ; Male ; Medical sciences ; Middle Aged ; Pharmacology. Drug treatments ; Streptozocin - administration & dosage</subject><ispartof>Cancer, 1985-08, Vol.56 (4), p.793-796</ispartof><rights>Copyright © 1985 American Cancer Society</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3276-d2e290147b73d863bc4870ad97da44021a64b5838deecc2204c94943d83f9a923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9296368$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3160455$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wilander, Erik</creatorcontrib><creatorcontrib>Bengtsson, Allan</creatorcontrib><creatorcontrib>Lindgren, Per G.</creatorcontrib><creatorcontrib>Lundqvist, Monalill</creatorcontrib><creatorcontrib>Norheim, Ingrid</creatorcontrib><creatorcontrib>Öberg, Kjell</creatorcontrib><title>Nuclear DNA in intestinal carcinoid tumors. A study before and after cytotoxin (streptozotocin and 5‐Fluorouracil) treatment</title><title>Cancer</title><addtitle>Cancer</addtitle><description>Imprint cytology specimens of metastases of intestinal carcinoids obtained by percutaneous biopsy were analysed cytofluorometrically with regard to nuclear DNA records. All untreated tumors (nine cases) exhibited diploid DNA values with a relatively low proliferative activity (less than 2% nuclei in Sphase region). The mean number of tetraploid cells was 5%. Cytofluorometry also was performed on five tumor metastases treated with the cytotoxin streptozotocin and 5‐fluorouracil. After treatment, an increase in the number of tetraploid cells (mean value, 30%) was noted, indicating that the cytotoxin treatment (possibly streptozotocin) on the tumor cells in vivo blocked progression from G2 to M phase. The current cytofluorometric analyses show that diploid nuclear DNA records and a low proliferative activity is a characteristic of malignant carcinoid tumors of the intestine. Due to regular DNA histograms in the carcinoid tumors, it is suggested that reliable studies are permitted of the effect of cytotoxins on the different phases of the cell cycle in vivo.</description><subject>Aged</subject><subject>Antineoplastic agents</subject><subject>Antineoplastic Combined Chemotherapy Protocols - therapeutic use</subject><subject>Biological and medical sciences</subject><subject>Carcinoid Tumor - drug therapy</subject><subject>Carcinoid Tumor - metabolism</subject><subject>Carcinoid Tumor - pathology</subject><subject>Cell Nucleus - metabolism</subject><subject>Chemotherapy</subject><subject>Diploidy</subject><subject>DNA - genetics</subject><subject>DNA - metabolism</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Fluorouracil - administration & dosage</subject><subject>Humans</subject><subject>Intestinal Neoplasms - drug therapy</subject><subject>Intestinal Neoplasms - metabolism</subject><subject>Intestinal Neoplasms - pathology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Pharmacology. Drug treatments</subject><subject>Streptozocin - administration & dosage</subject><issn>0008-543X</issn><issn>1097-0142</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkd9qFDEUxoModa0-gpALkfZi1vyfyVaEdWq1UHZBlIo3IZPJwMjMZE0ytOtF6SP0GfskZt11QS8EIRAO5_cdvnM-AN5iNMUIkVcYyTxDmJEjLAuOCsyPuZix17mks9n8_DQrF-VHUhDEBWJYvKFTNC2XJyS7fAAme_FDMEEIFRln9Mtj8CSEb6nMCacH4IDipOR8Am4Wo-ms9vB0MYftkF60IbaD7qDR3rSDa2sYx975MIVzGOJYr2FlG-ct1EMNdROth2YdXXTXSX8Uorer6H6kOql_Mfz-9u6sG513o9em7Y5hYnTs7RCfgkeN7oJ9tvsPweezd5_KD9nF8v15Ob_IDCW5yGpiiUw75VVO60LQyrAiR7qWea0ZQwRrwSpe0KK21hhCEDOSSZZY2kgtCT0EL7dzV959H9OGqm-DsV2nB-vGoHKxOaZACfy6BY13IXjbqJVve-3XCiO1CUdt7qs291W_w1FcKKZSOEqlcNSf4SiqkCqXiqjLNPz5zsVY9bbej96lkfovdn0djO4arwfThj0miRRUFAmzW-yq7ez6vwz-099fHfoT1Ty67g</recordid><startdate>19850815</startdate><enddate>19850815</enddate><creator>Wilander, Erik</creator><creator>Bengtsson, Allan</creator><creator>Lindgren, Per G.</creator><creator>Lundqvist, Monalill</creator><creator>Norheim, Ingrid</creator><creator>Öberg, Kjell</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19850815</creationdate><title>Nuclear DNA in intestinal carcinoid tumors. A study before and after cytotoxin (streptozotocin and 5‐Fluorouracil) treatment</title><author>Wilander, Erik ; Bengtsson, Allan ; Lindgren, Per G. ; Lundqvist, Monalill ; Norheim, Ingrid ; Öberg, Kjell</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3276-d2e290147b73d863bc4870ad97da44021a64b5838deecc2204c94943d83f9a923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Aged</topic><topic>Antineoplastic agents</topic><topic>Antineoplastic Combined Chemotherapy Protocols - therapeutic use</topic><topic>Biological and medical sciences</topic><topic>Carcinoid Tumor - drug therapy</topic><topic>Carcinoid Tumor - metabolism</topic><topic>Carcinoid Tumor - pathology</topic><topic>Cell Nucleus - metabolism</topic><topic>Chemotherapy</topic><topic>Diploidy</topic><topic>DNA - genetics</topic><topic>DNA - metabolism</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Fluorouracil - administration & dosage</topic><topic>Humans</topic><topic>Intestinal Neoplasms - drug therapy</topic><topic>Intestinal Neoplasms - metabolism</topic><topic>Intestinal Neoplasms - pathology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Pharmacology. Drug treatments</topic><topic>Streptozocin - administration & dosage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wilander, Erik</creatorcontrib><creatorcontrib>Bengtsson, Allan</creatorcontrib><creatorcontrib>Lindgren, Per G.</creatorcontrib><creatorcontrib>Lundqvist, Monalill</creatorcontrib><creatorcontrib>Norheim, Ingrid</creatorcontrib><creatorcontrib>Öberg, Kjell</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wilander, Erik</au><au>Bengtsson, Allan</au><au>Lindgren, Per G.</au><au>Lundqvist, Monalill</au><au>Norheim, Ingrid</au><au>Öberg, Kjell</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclear DNA in intestinal carcinoid tumors. A study before and after cytotoxin (streptozotocin and 5‐Fluorouracil) treatment</atitle><jtitle>Cancer</jtitle><addtitle>Cancer</addtitle><date>1985-08-15</date><risdate>1985</risdate><volume>56</volume><issue>4</issue><spage>793</spage><epage>796</epage><pages>793-796</pages><issn>0008-543X</issn><eissn>1097-0142</eissn><coden>CANCAR</coden><abstract>Imprint cytology specimens of metastases of intestinal carcinoids obtained by percutaneous biopsy were analysed cytofluorometrically with regard to nuclear DNA records. All untreated tumors (nine cases) exhibited diploid DNA values with a relatively low proliferative activity (less than 2% nuclei in Sphase region). The mean number of tetraploid cells was 5%. Cytofluorometry also was performed on five tumor metastases treated with the cytotoxin streptozotocin and 5‐fluorouracil. After treatment, an increase in the number of tetraploid cells (mean value, 30%) was noted, indicating that the cytotoxin treatment (possibly streptozotocin) on the tumor cells in vivo blocked progression from G2 to M phase. The current cytofluorometric analyses show that diploid nuclear DNA records and a low proliferative activity is a characteristic of malignant carcinoid tumors of the intestine. Due to regular DNA histograms in the carcinoid tumors, it is suggested that reliable studies are permitted of the effect of cytotoxins on the different phases of the cell cycle in vivo.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>3160455</pmid><doi>10.1002/1097-0142(19850815)56:4<793::AID-CNCR2820560416>3.0.CO;2-W</doi><tpages>4</tpages></addata></record> |
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| subjects | Aged Antineoplastic agents Antineoplastic Combined Chemotherapy Protocols - therapeutic use Biological and medical sciences Carcinoid Tumor - drug therapy Carcinoid Tumor - metabolism Carcinoid Tumor - pathology Cell Nucleus - metabolism Chemotherapy Diploidy DNA - genetics DNA - metabolism Female Flow Cytometry Fluorouracil - administration & dosage Humans Intestinal Neoplasms - drug therapy Intestinal Neoplasms - metabolism Intestinal Neoplasms - pathology Male Medical sciences Middle Aged Pharmacology. Drug treatments Streptozocin - administration & dosage |
| title | Nuclear DNA in intestinal carcinoid tumors. A study before and after cytotoxin (streptozotocin and 5‐Fluorouracil) treatment |
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