In vitro studies with mammalian cell lines and gum arabic-coated magnetic nanoparticles
Iron oxide magnetic nanoparticles (MNPs) were synthesized by the chemical co‐precipitation method and coated with gum arabic (GA) by physical adsorption and covalent attachment. Cultures of mammalian cell lines (HEK293, CHO and TE671) were grown in the presence of uncoated and GA‐coated MNPs. Cellul...
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| Veröffentlicht in: | Journal of molecular recognition 2010-11, Vol.23 (6), p.536-542 |
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| creator | Bicho, Ana Roque, Ana Cecília A. Cardoso, Ana Sofia Domingos, Patrícia Batalha, Íris Luz |
| description | Iron oxide magnetic nanoparticles (MNPs) were synthesized by the chemical co‐precipitation method and coated with gum arabic (GA) by physical adsorption and covalent attachment. Cultures of mammalian cell lines (HEK293, CHO and TE671) were grown in the presence of uncoated and GA‐coated MNPs. Cellular growth was followed by optical microscopy in order to assess the proportion of cells with particles, alterations in cellular density and the presence of debris. The in vitro assays demonstrated that cells from different origins are affected differently by the presence of the nanoparticles. Also, the methods followed for GA coating of MNPs endow distinct surface characteristics that probably underlie the observed differences when in contact with the cells. In general, the nanoparticles to which the GA was adsorbed had a smaller ability to attach to the cells' surface and to compromise the viability of the cultures. Copyright © 2010 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/jmr.1066 |
| format | Article |
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Cultures of mammalian cell lines (HEK293, CHO and TE671) were grown in the presence of uncoated and GA‐coated MNPs. Cellular growth was followed by optical microscopy in order to assess the proportion of cells with particles, alterations in cellular density and the presence of debris. The in vitro assays demonstrated that cells from different origins are affected differently by the presence of the nanoparticles. Also, the methods followed for GA coating of MNPs endow distinct surface characteristics that probably underlie the observed differences when in contact with the cells. In general, the nanoparticles to which the GA was adsorbed had a smaller ability to attach to the cells' surface and to compromise the viability of the cultures. 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Mol. Recognit</addtitle><description>Iron oxide magnetic nanoparticles (MNPs) were synthesized by the chemical co‐precipitation method and coated with gum arabic (GA) by physical adsorption and covalent attachment. Cultures of mammalian cell lines (HEK293, CHO and TE671) were grown in the presence of uncoated and GA‐coated MNPs. Cellular growth was followed by optical microscopy in order to assess the proportion of cells with particles, alterations in cellular density and the presence of debris. The in vitro assays demonstrated that cells from different origins are affected differently by the presence of the nanoparticles. Also, the methods followed for GA coating of MNPs endow distinct surface characteristics that probably underlie the observed differences when in contact with the cells. In general, the nanoparticles to which the GA was adsorbed had a smaller ability to attach to the cells' surface and to compromise the viability of the cultures. Copyright © 2010 John Wiley & Sons, Ltd.</description><subject>Animals</subject><subject>Cell Count</subject><subject>Cell Culture Techniques - instrumentation</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Line</subject><subject>Cell Proliferation - drug effects</subject><subject>Cellular</subject><subject>CHO</subject><subject>CHO Cells</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Coated Materials, Biocompatible - pharmacology</subject><subject>Contact</subject><subject>Cricetinae</subject><subject>Cricetulus</subject><subject>Culture</subject><subject>Density</subject><subject>Eukaryotic Cells - cytology</subject><subject>Eukaryotic Cells - drug effects</subject><subject>Genetic algorithms</subject><subject>gum arabic</subject><subject>Gum Arabic - chemistry</subject><subject>Gum Arabic - pharmacology</subject><subject>HEK293</subject><subject>Humans</subject><subject>In vitro testing</subject><subject>magnetic particles</subject><subject>Magnetics</subject><subject>Magnetite Nanoparticles - chemistry</subject><subject>mammalian cell lines</subject><subject>Mammals</subject><subject>Nanoparticles</subject><subject>Optical microscopy</subject><subject>TE671</subject><subject>Time Factors</subject><issn>0952-3499</issn><issn>1099-1352</issn><issn>1099-1352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90MtKxDAUBuAgio6j4BNIdrqp5tKkk6V46SjeUMFlyCSpRnsZk9Zx3t4Uq64UAufA-fgJPwA7GB1ghMjhS-XjwvkKGGEkRIIpI6tghAQjCU2F2ACbIbwgFG8MrYMNghHFacZH4PG8hu-u9Q0MbWecDXDh2mdYqapSpVM11LYsYenqeFG1gU9dBZVXM6cT3ajWmkifats6DWtVN3Pl41rasAXWClUGuz3MMbg_O304niaXN_n58dFlolPCeFJoo1OTck2R4cawjFBqUppqq0V8RBeYYMILyjliXEwyxm02Sy1jE8FmdAz2vlLnvnnrbGhl5UL_Y1Xbpgsy4wRRNEGTKPf_lX0hRCCB2S_VvgnB20LOvauUX0Yk-7plrFv2dUe6O6R2s8qaH_jdbwTJF1i40i7_DJIXV3dD4OBdaO3Hj1f-VfKMZkw-XucS3eZTimkuT-gnhoOXbQ</recordid><startdate>201011</startdate><enddate>201011</enddate><creator>Bicho, Ana</creator><creator>Roque, Ana Cecília A.</creator><creator>Cardoso, Ana Sofia</creator><creator>Domingos, Patrícia</creator><creator>Batalha, Íris Luz</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>201011</creationdate><title>In vitro studies with mammalian cell lines and gum arabic-coated magnetic nanoparticles</title><author>Bicho, Ana ; Roque, Ana Cecília A. ; Cardoso, Ana Sofia ; Domingos, Patrícia ; Batalha, Íris Luz</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4256-fcdc4d46c30d6dd57233d434cec9ec92cf12126f36605698756e7b4e55895b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Cell Count</topic><topic>Cell Culture Techniques - instrumentation</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Line</topic><topic>Cell Proliferation - drug effects</topic><topic>Cellular</topic><topic>CHO</topic><topic>CHO Cells</topic><topic>Coated Materials, Biocompatible - chemistry</topic><topic>Coated Materials, Biocompatible - pharmacology</topic><topic>Contact</topic><topic>Cricetinae</topic><topic>Cricetulus</topic><topic>Culture</topic><topic>Density</topic><topic>Eukaryotic Cells - cytology</topic><topic>Eukaryotic Cells - drug effects</topic><topic>Genetic algorithms</topic><topic>gum arabic</topic><topic>Gum Arabic - chemistry</topic><topic>Gum Arabic - pharmacology</topic><topic>HEK293</topic><topic>Humans</topic><topic>In vitro testing</topic><topic>magnetic particles</topic><topic>Magnetics</topic><topic>Magnetite Nanoparticles - chemistry</topic><topic>mammalian cell lines</topic><topic>Mammals</topic><topic>Nanoparticles</topic><topic>Optical microscopy</topic><topic>TE671</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bicho, Ana</creatorcontrib><creatorcontrib>Roque, Ana Cecília A.</creatorcontrib><creatorcontrib>Cardoso, Ana Sofia</creatorcontrib><creatorcontrib>Domingos, Patrícia</creatorcontrib><creatorcontrib>Batalha, Íris Luz</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular recognition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bicho, Ana</au><au>Roque, Ana Cecília A.</au><au>Cardoso, Ana Sofia</au><au>Domingos, Patrícia</au><au>Batalha, Íris Luz</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro studies with mammalian cell lines and gum arabic-coated magnetic nanoparticles</atitle><jtitle>Journal of molecular recognition</jtitle><addtitle>J. Mol. Recognit</addtitle><date>2010-11</date><risdate>2010</risdate><volume>23</volume><issue>6</issue><spage>536</spage><epage>542</epage><pages>536-542</pages><issn>0952-3499</issn><issn>1099-1352</issn><eissn>1099-1352</eissn><abstract>Iron oxide magnetic nanoparticles (MNPs) were synthesized by the chemical co‐precipitation method and coated with gum arabic (GA) by physical adsorption and covalent attachment. Cultures of mammalian cell lines (HEK293, CHO and TE671) were grown in the presence of uncoated and GA‐coated MNPs. Cellular growth was followed by optical microscopy in order to assess the proportion of cells with particles, alterations in cellular density and the presence of debris. The in vitro assays demonstrated that cells from different origins are affected differently by the presence of the nanoparticles. Also, the methods followed for GA coating of MNPs endow distinct surface characteristics that probably underlie the observed differences when in contact with the cells. In general, the nanoparticles to which the GA was adsorbed had a smaller ability to attach to the cells' surface and to compromise the viability of the cultures. Copyright © 2010 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>21031476</pmid><doi>10.1002/jmr.1066</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Cell Count Cell Culture Techniques - instrumentation Cell Culture Techniques - methods Cell Line Cell Proliferation - drug effects Cellular CHO CHO Cells Coated Materials, Biocompatible - chemistry Coated Materials, Biocompatible - pharmacology Contact Cricetinae Cricetulus Culture Density Eukaryotic Cells - cytology Eukaryotic Cells - drug effects Genetic algorithms gum arabic Gum Arabic - chemistry Gum Arabic - pharmacology HEK293 Humans In vitro testing magnetic particles Magnetics Magnetite Nanoparticles - chemistry mammalian cell lines Mammals Nanoparticles Optical microscopy TE671 Time Factors |
| title | In vitro studies with mammalian cell lines and gum arabic-coated magnetic nanoparticles |
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