Staining with Highly Sensitive Coomassie Brilliant Blue SeePico™ Stain after Flamingo™ Fluorescent Gel Stain Is Useful for Cancer Proteomic Analysis by Means of Two-dimensional Gel Electrophoresis

Highly sensitive Coomassie brilliant blue SeePico™ Stain was applied for proteomic analysis using two-dimensional gel electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). After staining with Flamingo™ Fluorescent Gel Stain, the images of the protein spots were analyz...

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Veröffentlicht in:	Anticancer research 2010-10, Vol.30 (10), p.4001-4005
Hauptverfasser:	KURAMITSU, Yasuhiro, HAYASHI, Eiko, OKADA, Futoshi, XIULIAN ZHANG, TANAKA, Toshiyuki, UEYAMA, Yoshiya, NAKAMURA, Kazuyuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Biological and medical sciences Cell Line, Tumor Chromatography, Liquid Electrophoresis, Gel, Two-Dimensional - methods Fibrosarcoma - chemistry Fibrosarcoma - metabolism Indicators and Reagents - chemistry Medical sciences Mice Molecular Sequence Data Neoplasm Proteins - analysis Neoplasm Proteins - biosynthesis Proteome - analysis Proteomics - methods Rosaniline Dyes - chemistry Staining and Labeling - methods Tandem Mass Spectrometry Tumors
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