Inhibition of carbonic anhydrase in dog plasma

Using a pH stat method, we measured the activity of carbonic anhydrase (CA) from dog erythrocytes in the presence of various amounts of dog plasma. A plasma factor appeared to be able to inhibit about 86% of the total CA activity, corresponding to the relative activity of CA II. Naiodoacetate was sh...

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Veröffentlicht in:	Pflügers Archiv 1985-04, Vol.403 (4), p.344-347
Hauptverfasser:	RISPENS, P, HESSELS, J, ZWART, A, ZIJLSTRA, W. G
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Blood gas. Hemoglobin. Myoglobin. Hemotissulary gas exchange. Acid-base balance Carbonic Anhydrase Inhibitors - blood Carbonic Anhydrase Inhibitors - pharmacology Chlorides - pharmacology Dogs Fluorides - pharmacology Fundamental and applied biological sciences. Psychology Hemolysis Iodoacetates - pharmacology Iodoacetic Acid Vertebrates: respiratory system
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Beschreibung
Zusammenfassung:	Using a pH stat method, we measured the activity of carbonic anhydrase (CA) from dog erythrocytes in the presence of various amounts of dog plasma. A plasma factor appeared to be able to inhibit about 86% of the total CA activity, corresponding to the relative activity of CA II. Naiodoacetate was shown to inhibit the total CA activity up to about 13%, corresponding to the relative activity of CA I. C1- inhibited the total CA activity up to about 20%, presumably mainly through its strong influence on Ca I. It is concluded that with a degree of haemolysis of up to 3%, no appreciable plasma CA activity will occur.
ISSN:	0031-6768 1432-2013
DOI:	10.1007/BF00589244