Estrogen response in the hFOB 1.19 human fetal osteoblastic cell line stably transfected with the human estrogen receptor gene

The gene coding for the human wild‐type estrogen receptor (ER) was stably transfected into the human fetal osteoblastic cell line hFOB 1.19, a clonal cell line which is conditionally immortilized with a temperature sensitive mutant of SV40 large T antigen (tsA58). Five subclones were obtained which...

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Veröffentlicht in:	Journal of cellular biochemistry 1995-10, Vol.59 (2), p.193-201
Hauptverfasser:	Harris, Steven A., Tau, Kimberly R., Enger, Robert J., Toft, David O., Riggs, B. Lawrence, Spelsberg, Thomas C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Cell Line Estradiol - metabolism estrogen receptor Fetus - physiology Gene Expression Regulation, Developmental - physiology Humans Luciferases - biosynthesis Molecular Sequence Data osteoblasts Osteoblasts - metabolism Receptors, Estrogen - genetics RNA, Messenger - biosynthesis stable transfection SV40 large T antigen Transfection
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