alpha2-adrenergic agonist-induced inhibition of cyclic AMP formation in transfected cell lines using a microtiter-based scintillation proximity assay

Human embryonic kidney cells (HEK-7) were transfected with the gene for the human alpha2C receptor (alpha2C4). Cells were grown in 96-well microtiter plates and cyclic AMP levels were measured by scintillation proximity assay, a modified radioimmunoassay technique. Radioactivity was quantified using...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Journal of receptors and signal transduction 1995-01, Vol.15 (1-4), p.557-579
Hauptverfasser:	Hancock, A A, Vodenlich, A D, Maldonado, C, Janis, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Adrenergic alpha-2 Receptor Agonists Cell Line Clone Cells Clonidine - pharmacology Colforsin - pharmacology Cyclic AMP - biosynthesis Humans Norepinephrine - pharmacology Phentolamine - pharmacology Radioligand Assay Receptors, Adrenergic, alpha-2 - genetics Receptors, Adrenergic, alpha-2 - metabolism Scintillation Counting - methods Transfection
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!