Insertion of eukaryotic DNA into the Bacillus subtilis genome by means of a temperature-sensitive plasmid vector
A hybrid temperature-sensitive plasmid capable of integration into the Bacillus subtilis genome was constructed. By using this vector, we inserted a 3.2-kb fragment of eukaryotic DNA (wheat ‘Chinese Spring’) into the bacterial genome. The fragment of wheat DNA was stably retained and replicated as a...
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| Veröffentlicht in: | Gene 1985, Vol.34 (1), p.39-46 |
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| container_end_page | 46 |
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| container_issue | 1 |
| container_start_page | 39 |
| container_title | Gene |
| container_volume | 34 |
| creator | Prozorov, Alexander A. Bashkirov, Vladimir I. Khasanov, Fuat K. Glumova, Ekaterina F. Irich, Viktor Y. |
| description | A hybrid temperature-sensitive plasmid capable of integration into the
Bacillus subtilis genome was constructed. By using this vector, we inserted a 3.2-kb fragment of eukaryotic DNA (wheat ‘Chinese Spring’) into the bacterial genome. The fragment of wheat DNA was stably retained and replicated as a part of the bacterial genome. The position of the integrated plasmid in the
B. subtilis genome was mapped, as was the site in wheat DNA insert on plasmid at which the integration occurred. |
| doi_str_mv | 10.1016/0378-1119(85)90292-6 |
| format | Article |
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Bacillus subtilis genome was constructed. By using this vector, we inserted a 3.2-kb fragment of eukaryotic DNA (wheat ‘Chinese Spring’) into the bacterial genome. The fragment of wheat DNA was stably retained and replicated as a part of the bacterial genome. The position of the integrated plasmid in the
B. subtilis genome was mapped, as was the site in wheat DNA insert on plasmid at which the integration occurred.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(85)90292-6</identifier><identifier>PMID: 3924736</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>Bacillus subtilis - genetics ; Bacillus subtilis - ultrastructure ; Base Sequence ; Biological and medical sciences ; Biotechnology ; Chromosomes, Bacterial - metabolism ; Cloning, Molecular ; DNA - metabolism ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Genetic Vectors ; map position ; Methods. Procedures. Technologies ; Plasmids ; Recombinant DNA ; Recombination, Genetic ; site of integration ; Temperature ; Triticum - genetics ; Vectors (cloning, transfer, expression). Insertion sequences and transposons ; wheat DNA cloning</subject><ispartof>Gene, 1985, Vol.34 (1), p.39-46</ispartof><rights>1985</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-ace58b8586c7337e26d4812095b89fcf06578ae96bddd9f6b9bc3e2c49c3d6d43</citedby><cites>FETCH-LOGICAL-c422t-ace58b8586c7337e26d4812095b89fcf06578ae96bddd9f6b9bc3e2c49c3d6d43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(85)90292-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3541,4014,27914,27915,27916,45986</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8458136$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3924736$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Prozorov, Alexander A.</creatorcontrib><creatorcontrib>Bashkirov, Vladimir I.</creatorcontrib><creatorcontrib>Khasanov, Fuat K.</creatorcontrib><creatorcontrib>Glumova, Ekaterina F.</creatorcontrib><creatorcontrib>Irich, Viktor Y.</creatorcontrib><title>Insertion of eukaryotic DNA into the Bacillus subtilis genome by means of a temperature-sensitive plasmid vector</title><title>Gene</title><addtitle>Gene</addtitle><description>A hybrid temperature-sensitive plasmid capable of integration into the
Bacillus subtilis genome was constructed. By using this vector, we inserted a 3.2-kb fragment of eukaryotic DNA (wheat ‘Chinese Spring’) into the bacterial genome. The fragment of wheat DNA was stably retained and replicated as a part of the bacterial genome. The position of the integrated plasmid in the
B. subtilis genome was mapped, as was the site in wheat DNA insert on plasmid at which the integration occurred.</description><subject>Bacillus subtilis - genetics</subject><subject>Bacillus subtilis - ultrastructure</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Chromosomes, Bacterial - metabolism</subject><subject>Cloning, Molecular</subject><subject>DNA - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic Vectors</subject><subject>map position</subject><subject>Methods. Procedures. Technologies</subject><subject>Plasmids</subject><subject>Recombinant DNA</subject><subject>Recombination, Genetic</subject><subject>site of integration</subject><subject>Temperature</subject><subject>Triticum - genetics</subject><subject>Vectors (cloning, transfer, expression). Insertion sequences and transposons</subject><subject>wheat DNA cloning</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFu1DAQhi0EKtvCG4DkA0LlkGInsWNfkEqBUqkqFzhbjj0BQ2KnHmelvj3Z7mqPncsc5vt_jT5C3nB2wRmXH1nTqYpzrs-V-KBZretKPiMbrjpdMdao52RzRF6SU8S_bB0h6hNy0ui67Rq5IfNNRMglpEjTQGH5Z_NDKsHRL3eXNMSSaPkD9LN1YRwXpLj0JYwB6W-IaQLaP9AJbMRd2NIC0wzZliVDhRAxlLAFOo8Wp-DpFlxJ-RV5MdgR4fVhn5Ff377-vPpe3f64vrm6vK1cW9elsg6E6pVQ0nVN00Etfat4zbTolR7cwKTolAUte--9HmSve9dA7VrtGr-yzRl5v--dc7pfAIuZAjoYRxshLWg6yWWnNV_Bdg-6nBAzDGbOYVo1GM7MTrTZWTQ7i0YJ8yjayDX29tC_9BP4Y-hgdr2_O9wtOjsO2UYX8IipVij-iH3aY7C62AbIBl2A6MCHvPoyPoWn__gPutibUg</recordid><startdate>1985</startdate><enddate>1985</enddate><creator>Prozorov, Alexander A.</creator><creator>Bashkirov, Vladimir I.</creator><creator>Khasanov, Fuat K.</creator><creator>Glumova, Ekaterina F.</creator><creator>Irich, Viktor Y.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1985</creationdate><title>Insertion of eukaryotic DNA into the Bacillus subtilis genome by means of a temperature-sensitive plasmid vector</title><author>Prozorov, Alexander A. ; Bashkirov, Vladimir I. ; Khasanov, Fuat K. ; Glumova, Ekaterina F. ; Irich, Viktor Y.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-ace58b8586c7337e26d4812095b89fcf06578ae96bddd9f6b9bc3e2c49c3d6d43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Bacillus subtilis - genetics</topic><topic>Bacillus subtilis - ultrastructure</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Chromosomes, Bacterial - metabolism</topic><topic>Cloning, Molecular</topic><topic>DNA - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic Vectors</topic><topic>map position</topic><topic>Methods. Procedures. Technologies</topic><topic>Plasmids</topic><topic>Recombinant DNA</topic><topic>Recombination, Genetic</topic><topic>site of integration</topic><topic>Temperature</topic><topic>Triticum - genetics</topic><topic>Vectors (cloning, transfer, expression). Insertion sequences and transposons</topic><topic>wheat DNA cloning</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Prozorov, Alexander A.</creatorcontrib><creatorcontrib>Bashkirov, Vladimir I.</creatorcontrib><creatorcontrib>Khasanov, Fuat K.</creatorcontrib><creatorcontrib>Glumova, Ekaterina F.</creatorcontrib><creatorcontrib>Irich, Viktor Y.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Prozorov, Alexander A.</au><au>Bashkirov, Vladimir I.</au><au>Khasanov, Fuat K.</au><au>Glumova, Ekaterina F.</au><au>Irich, Viktor Y.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Insertion of eukaryotic DNA into the Bacillus subtilis genome by means of a temperature-sensitive plasmid vector</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1985</date><risdate>1985</risdate><volume>34</volume><issue>1</issue><spage>39</spage><epage>46</epage><pages>39-46</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>A hybrid temperature-sensitive plasmid capable of integration into the
Bacillus subtilis genome was constructed. By using this vector, we inserted a 3.2-kb fragment of eukaryotic DNA (wheat ‘Chinese Spring’) into the bacterial genome. The fragment of wheat DNA was stably retained and replicated as a part of the bacterial genome. The position of the integrated plasmid in the
B. subtilis genome was mapped, as was the site in wheat DNA insert on plasmid at which the integration occurred.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>3924736</pmid><doi>10.1016/0378-1119(85)90292-6</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1985, Vol.34 (1), p.39-46 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76167991 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Bacillus subtilis - genetics Bacillus subtilis - ultrastructure Base Sequence Biological and medical sciences Biotechnology Chromosomes, Bacterial - metabolism Cloning, Molecular DNA - metabolism Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetic Vectors map position Methods. Procedures. Technologies Plasmids Recombinant DNA Recombination, Genetic site of integration Temperature Triticum - genetics Vectors (cloning, transfer, expression). Insertion sequences and transposons wheat DNA cloning |
| title | Insertion of eukaryotic DNA into the Bacillus subtilis genome by means of a temperature-sensitive plasmid vector |
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