Effect of dehydroepiandrosterone on pentose phosphate pathway activity in the rat colon
1. 1. The effects of fasting and fasting followed by refeeding on the activities of the oxidative pentose pathway (OPP) and the non-oxidative pentose pathway (NOPP) were estimated by the rate of production of 14CO 2 from [l- 14C] glucose in isolated rat colonocytes, and the production of hexose 6-ph...
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| Veröffentlicht in: | International journal of biochemistry 1993-11, Vol.25 (11), p.1601-1607 |
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| container_title | International journal of biochemistry |
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| creator | Butler, R.N. Butler, W.J. Antoniou, D. Pascoe, V. |
| description | 1.
1. The effects of fasting and fasting followed by refeeding on the activities of the oxidative pentose pathway (OPP) and the non-oxidative pentose pathway (NOPP) were estimated by the rate of production of
14CO
2 from [l-
14C] glucose in isolated rat colonocytes, and the production of hexose 6-phosphates from ribose 5-phosphate in rat colonic cytosols, respectively.
2.
2. The OPP activity in colonocytes from rats in the fasted state was 50% lower when compared to colonocytes from rats refed after a fast. This indicated induction of the rate-limiting enzyme of the OPP, glucose 6-P dehydrogenase (G6-PDH) in the latter instance. No effect on the maximal catalytic activity of the enzymes of the NOPP was seen in colonocytes from rats refed after a fast compared with colonocytes from rats in the fasted state.
3.
3. Isolated colonocytes obtained from the distal colon of rats refed after a fast, showed a significant decrease (30%) in OPP activity when incubated with 50 μ M dehydroepiandrosterone (DHEA). A similar degree of inhibition was seen with 10 mM butyrate (
P |
| doi_str_mv | 10.1016/0020-711X(93)90518-J |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76165054</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0020711X9390518J</els_id><sourcerecordid>76165054</sourcerecordid><originalsourceid>FETCH-LOGICAL-c386t-9396d076b23d8ca4dce9c5bd0717121ba8adbcf660a4f4f225d19b3b5152ce0d3</originalsourceid><addsrcrecordid>eNp9kE1vGyEQQDm0SlM3_6CVOFRRc9gE2GUNl0hRlOZDkXpJlNwQC4OWag1bwKn874try8ecZjTzZjTzEPpKyTkltL8ghJFmSenrD9meScKpaB4-oOND-RP6nPNvQqgUHT1CR4IJQZg8Ri83zoEpODpsYdzYFGH2OtSYC6QYAMeAZwglZsDzGPM86lIzXca_eoO1Kf7Nlw32AZcRcNIFmzjF8AV9dHrKcLKPC_T88-bp-q55_HV7f3312JhW9KWRrewtWfYDa60wurMGpOFDLdElZXTQQtvBuL4nunOdY4xbKod24JQzA8S2C3S62zun-GcNuaiVzwamSQeI66yWPe054V0Fux1o6ms5gVNz8iudNooStXWotrLUVpaSrfrvUD3UsW_7_ethBfYwtBdY-9_3fZ2NnlzSwfh8wFrRccJ4xS53GFQXbx6SysZDMGB9qvqVjf79O_4BcnmRRg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76165054</pqid></control><display><type>article</type><title>Effect of dehydroepiandrosterone on pentose phosphate pathway activity in the rat colon</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Butler, R.N. ; Butler, W.J. ; Antoniou, D. ; Pascoe, V.</creator><creatorcontrib>Butler, R.N. ; Butler, W.J. ; Antoniou, D. ; Pascoe, V.</creatorcontrib><description>1.
1. The effects of fasting and fasting followed by refeeding on the activities of the oxidative pentose pathway (OPP) and the non-oxidative pentose pathway (NOPP) were estimated by the rate of production of
14CO
2 from [l-
14C] glucose in isolated rat colonocytes, and the production of hexose 6-phosphates from ribose 5-phosphate in rat colonic cytosols, respectively.
2.
2. The OPP activity in colonocytes from rats in the fasted state was 50% lower when compared to colonocytes from rats refed after a fast. This indicated induction of the rate-limiting enzyme of the OPP, glucose 6-P dehydrogenase (G6-PDH) in the latter instance. No effect on the maximal catalytic activity of the enzymes of the NOPP was seen in colonocytes from rats refed after a fast compared with colonocytes from rats in the fasted state.
3.
3. Isolated colonocytes obtained from the distal colon of rats refed after a fast, showed a significant decrease (30%) in OPP activity when incubated with 50 μ M dehydroepiandrosterone (DHEA). A similar degree of inhibition was seen with 10 mM butyrate (
P <0.05). In contrast, using colonie cytosols, both DHEA and butyrate had no effect on the maximal catalytic activity of the NOPP.
4.
4. Intraperitoneal injection (i.p.) of DHEA in rats refed after a fast showed a significant increase in the maximal catalytic activity of the NOPP in the distal colon (46%;
P <0.05). A similar elevation in the maximal catalytic activity of the NOPP was seen in the distal colon of DHEA treated pair-fed rats (43%;
P < 0.05). No significant change was seen in maximal catalytic activity of the NOPP in colonocytes obtained from the proximal colon of DHEA treated rats in both
ad libitum fed and pair-fed rats.
5.
5. DHEA administration is postulated to increase the maximal catalytic activity of the NOPP as a compensatory response to a lowered OPP activity. This increased potential for glucose flux through the NOPP can presumably replace the deficit in supply of phosphorylated sugars needed for the actively dividing colonocytes in the distal colon.</description><identifier>ISSN: 0020-711X</identifier><identifier>DOI: 10.1016/0020-711X(93)90518-J</identifier><identifier>PMID: 8288029</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>Adrenals. Interrenals ; Adrenocortical hormones. Regulation ; Animals ; Biological and medical sciences ; Colon - drug effects ; Colon - metabolism ; Dehydroepiandrosterone - pharmacology ; Female ; Fundamental and applied biological sciences. Psychology ; Oxidation-Reduction ; Pentose Phosphate Pathway - drug effects ; Rats ; Rats, Sprague-Dawley ; Starvation ; Vertebrates: endocrinology</subject><ispartof>International journal of biochemistry, 1993-11, Vol.25 (11), p.1601-1607</ispartof><rights>1993</rights><rights>1994 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-9396d076b23d8ca4dce9c5bd0717121ba8adbcf660a4f4f225d19b3b5152ce0d3</citedby><cites>FETCH-LOGICAL-c386t-9396d076b23d8ca4dce9c5bd0717121ba8adbcf660a4f4f225d19b3b5152ce0d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3845025$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8288029$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Butler, R.N.</creatorcontrib><creatorcontrib>Butler, W.J.</creatorcontrib><creatorcontrib>Antoniou, D.</creatorcontrib><creatorcontrib>Pascoe, V.</creatorcontrib><title>Effect of dehydroepiandrosterone on pentose phosphate pathway activity in the rat colon</title><title>International journal of biochemistry</title><addtitle>Int J Biochem</addtitle><description>1.
1. The effects of fasting and fasting followed by refeeding on the activities of the oxidative pentose pathway (OPP) and the non-oxidative pentose pathway (NOPP) were estimated by the rate of production of
14CO
2 from [l-
14C] glucose in isolated rat colonocytes, and the production of hexose 6-phosphates from ribose 5-phosphate in rat colonic cytosols, respectively.
2.
2. The OPP activity in colonocytes from rats in the fasted state was 50% lower when compared to colonocytes from rats refed after a fast. This indicated induction of the rate-limiting enzyme of the OPP, glucose 6-P dehydrogenase (G6-PDH) in the latter instance. No effect on the maximal catalytic activity of the enzymes of the NOPP was seen in colonocytes from rats refed after a fast compared with colonocytes from rats in the fasted state.
3.
3. Isolated colonocytes obtained from the distal colon of rats refed after a fast, showed a significant decrease (30%) in OPP activity when incubated with 50 μ M dehydroepiandrosterone (DHEA). A similar degree of inhibition was seen with 10 mM butyrate (
P <0.05). In contrast, using colonie cytosols, both DHEA and butyrate had no effect on the maximal catalytic activity of the NOPP.
4.
4. Intraperitoneal injection (i.p.) of DHEA in rats refed after a fast showed a significant increase in the maximal catalytic activity of the NOPP in the distal colon (46%;
P <0.05). A similar elevation in the maximal catalytic activity of the NOPP was seen in the distal colon of DHEA treated pair-fed rats (43%;
P < 0.05). No significant change was seen in maximal catalytic activity of the NOPP in colonocytes obtained from the proximal colon of DHEA treated rats in both
ad libitum fed and pair-fed rats.
5.
5. DHEA administration is postulated to increase the maximal catalytic activity of the NOPP as a compensatory response to a lowered OPP activity. This increased potential for glucose flux through the NOPP can presumably replace the deficit in supply of phosphorylated sugars needed for the actively dividing colonocytes in the distal colon.</description><subject>Adrenals. Interrenals</subject><subject>Adrenocortical hormones. Regulation</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Colon - drug effects</subject><subject>Colon - metabolism</subject><subject>Dehydroepiandrosterone - pharmacology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Oxidation-Reduction</subject><subject>Pentose Phosphate Pathway - drug effects</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Starvation</subject><subject>Vertebrates: endocrinology</subject><issn>0020-711X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1vGyEQQDm0SlM3_6CVOFRRc9gE2GUNl0hRlOZDkXpJlNwQC4OWag1bwKn874try8ecZjTzZjTzEPpKyTkltL8ghJFmSenrD9meScKpaB4-oOND-RP6nPNvQqgUHT1CR4IJQZg8Ri83zoEpODpsYdzYFGH2OtSYC6QYAMeAZwglZsDzGPM86lIzXca_eoO1Kf7Nlw32AZcRcNIFmzjF8AV9dHrKcLKPC_T88-bp-q55_HV7f3312JhW9KWRrewtWfYDa60wurMGpOFDLdElZXTQQtvBuL4nunOdY4xbKod24JQzA8S2C3S62zun-GcNuaiVzwamSQeI66yWPe054V0Fux1o6ms5gVNz8iudNooStXWotrLUVpaSrfrvUD3UsW_7_ethBfYwtBdY-9_3fZ2NnlzSwfh8wFrRccJ4xS53GFQXbx6SysZDMGB9qvqVjf79O_4BcnmRRg</recordid><startdate>19931101</startdate><enddate>19931101</enddate><creator>Butler, R.N.</creator><creator>Butler, W.J.</creator><creator>Antoniou, D.</creator><creator>Pascoe, V.</creator><general>Elsevier B.V</general><general>Pergamon</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19931101</creationdate><title>Effect of dehydroepiandrosterone on pentose phosphate pathway activity in the rat colon</title><author>Butler, R.N. ; Butler, W.J. ; Antoniou, D. ; Pascoe, V.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-9396d076b23d8ca4dce9c5bd0717121ba8adbcf660a4f4f225d19b3b5152ce0d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adrenals. Interrenals</topic><topic>Adrenocortical hormones. Regulation</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Colon - drug effects</topic><topic>Colon - metabolism</topic><topic>Dehydroepiandrosterone - pharmacology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Oxidation-Reduction</topic><topic>Pentose Phosphate Pathway - drug effects</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Starvation</topic><topic>Vertebrates: endocrinology</topic><toplevel>online_resources</toplevel><creatorcontrib>Butler, R.N.</creatorcontrib><creatorcontrib>Butler, W.J.</creatorcontrib><creatorcontrib>Antoniou, D.</creatorcontrib><creatorcontrib>Pascoe, V.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Butler, R.N.</au><au>Butler, W.J.</au><au>Antoniou, D.</au><au>Pascoe, V.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of dehydroepiandrosterone on pentose phosphate pathway activity in the rat colon</atitle><jtitle>International journal of biochemistry</jtitle><addtitle>Int J Biochem</addtitle><date>1993-11-01</date><risdate>1993</risdate><volume>25</volume><issue>11</issue><spage>1601</spage><epage>1607</epage><pages>1601-1607</pages><issn>0020-711X</issn><abstract>1.
1. The effects of fasting and fasting followed by refeeding on the activities of the oxidative pentose pathway (OPP) and the non-oxidative pentose pathway (NOPP) were estimated by the rate of production of
14CO
2 from [l-
14C] glucose in isolated rat colonocytes, and the production of hexose 6-phosphates from ribose 5-phosphate in rat colonic cytosols, respectively.
2.
2. The OPP activity in colonocytes from rats in the fasted state was 50% lower when compared to colonocytes from rats refed after a fast. This indicated induction of the rate-limiting enzyme of the OPP, glucose 6-P dehydrogenase (G6-PDH) in the latter instance. No effect on the maximal catalytic activity of the enzymes of the NOPP was seen in colonocytes from rats refed after a fast compared with colonocytes from rats in the fasted state.
3.
3. Isolated colonocytes obtained from the distal colon of rats refed after a fast, showed a significant decrease (30%) in OPP activity when incubated with 50 μ M dehydroepiandrosterone (DHEA). A similar degree of inhibition was seen with 10 mM butyrate (
P <0.05). In contrast, using colonie cytosols, both DHEA and butyrate had no effect on the maximal catalytic activity of the NOPP.
4.
4. Intraperitoneal injection (i.p.) of DHEA in rats refed after a fast showed a significant increase in the maximal catalytic activity of the NOPP in the distal colon (46%;
P <0.05). A similar elevation in the maximal catalytic activity of the NOPP was seen in the distal colon of DHEA treated pair-fed rats (43%;
P < 0.05). No significant change was seen in maximal catalytic activity of the NOPP in colonocytes obtained from the proximal colon of DHEA treated rats in both
ad libitum fed and pair-fed rats.
5.
5. DHEA administration is postulated to increase the maximal catalytic activity of the NOPP as a compensatory response to a lowered OPP activity. This increased potential for glucose flux through the NOPP can presumably replace the deficit in supply of phosphorylated sugars needed for the actively dividing colonocytes in the distal colon.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>8288029</pmid><doi>10.1016/0020-711X(93)90518-J</doi><tpages>7</tpages></addata></record> |
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| subjects | Adrenals. Interrenals Adrenocortical hormones. Regulation Animals Biological and medical sciences Colon - drug effects Colon - metabolism Dehydroepiandrosterone - pharmacology Female Fundamental and applied biological sciences. Psychology Oxidation-Reduction Pentose Phosphate Pathway - drug effects Rats Rats, Sprague-Dawley Starvation Vertebrates: endocrinology |
| title | Effect of dehydroepiandrosterone on pentose phosphate pathway activity in the rat colon |
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