Enzyme Labeling of Steroids by the N-Succinimidyl Ester Method. Preparation of Horseradish Peroxidase-Labeled Antigen for Use in Enzyme Immunoassay

Enzyme labeling of a steroid with horseradish peroxidase by the N-succinimidyl ester method has been investigated in comparison with that with β-galactosidase. The reaction of the activated ester of 4-hydroxytestosterone 4-hemiglutarate with horseradish peroxidase provided a labeled antigen showing...

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Veröffentlicht in:	Chemical & pharmaceutical bulletin 1985/01/25, Vol.33(1), pp.249-255
Hauptverfasser:	HOSODA, HIROSHI, KARUBE, TOMOKO, KOBAYASHI, NORIHIRO, NAMBARA, TOSHIO
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Biological and medical sciences Esters General pharmacology Horseradish Peroxidase Immunoenzyme Techniques Medical sciences Pharmacology. Drug treatments sensitivity Steroids - analysis Succinimides Testosterone - analysis
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