Semiconductor laser-induced fluorescence detection in capillary electrophoresis using a cyanine dye

Cy5, an activated carboxyl cyanine fluorophore, was characterized by capillary electrophoresis (CE) using a semiconductor laser at 652 nm to induce fluorescence. Hydrolysis of the activated Cy5 in the presence of ammonia results in the formation of a mono- and diamide and a dicarboxylic acid. A Cy5-...

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Veröffentlicht in:	Journal of Chromatography A 1993-10, Vol.652 (2), p.355-360
Hauptverfasser:	Chen, Fu-Tai A., Tusak, Anton, Pentoney, Stephen, Konrad, Ken, Lew, Clarence, Koh, Ed, Sternberg, James
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Carbocyanines - chemistry Coloring Agents DNA Primers Electrophoresis - methods Lasers Molecular Sequence Data Semiconductors Spectrometry, Fluorescence - methods Spectrophotometry, Ultraviolet
Online-Zugang:	Volltext
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