Acetylcholine release in the cat caudate nucleus measured with the choline oxidase method
A chemiluminescent assay for the estimation of acetylcholine (ACh) was used to measure ACh release in caudate nuclei (CN) of halothane-anaesthetized cats implanted with push-pull cannulae. The validity of the entire experimental approach used was shown by the fact that ACh release was calcium-depend...
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| Veröffentlicht in: | European journal of pharmacology 1985-03, Vol.110 (1), p.81-87 |
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| container_title | European journal of pharmacology |
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| creator | Morot-Gaudry, Yvette Romo, Ranulfo Lesbats, Bernard Chéramy, André Godeheu, Gérard Glowinski, Jacques Israël, Maurice |
| description | A chemiluminescent assay for the estimation of acetylcholine (ACh) was used to measure ACh release in caudate nuclei (CN) of halothane-anaesthetized cats implanted with push-pull cannulae. The validity of the entire experimental approach used was shown by the fact that ACh release was calcium-dependent and was increased by depolarizing agents (potassium ions, veratridine) as well as by atropine. The effects of GABA (10
−5 M, 30 min) unilateral application into the ventralis medialis and ventralis lateralis thalamic nuclei on ACh release in both CN were then examined. This treatment, known to increase DA release bilaterally, decreased ACh release in both CN. These data further reveal the role of thalamic nuclei in the bilateral regulation of the activity of neurons identified within the basal ganglia and are discussed in the light of the well-known inhibitory influence of nigrostriatal DA neurons on striatal cholinergic neurons. |
| doi_str_mv | 10.1016/0014-2999(85)90031-7 |
| format | Article |
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−5 M, 30 min) unilateral application into the ventralis medialis and ventralis lateralis thalamic nuclei on ACh release in both CN were then examined. This treatment, known to increase DA release bilaterally, decreased ACh release in both CN. These data further reveal the role of thalamic nuclei in the bilateral regulation of the activity of neurons identified within the basal ganglia and are discussed in the light of the well-known inhibitory influence of nigrostriatal DA neurons on striatal cholinergic neurons.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/0014-2999(85)90031-7</identifier><identifier>PMID: 3891381</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acetylcholine - metabolism ; Acetylcholine release ; Alcohol Oxidoreductases ; Animals ; Biological and medical sciences ; Cats ; Caudate nucleus ; Caudate Nucleus - metabolism ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Chemiluminescent assay ; Female ; Fundamental and applied biological sciences. Psychology ; gamma-Aminobutyric Acid - pharmacology ; In Vitro Techniques ; Luminescent Measurements ; Male ; Methods ; Push-pull cannula ; Thalamus ; Thalamus - drug effects ; Vertebrates: nervous system and sense organs</subject><ispartof>European journal of pharmacology, 1985-03, Vol.110 (1), p.81-87</ispartof><rights>1985</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-b70ab204b23d2e2806ff1a46a993db655642d7952391d2a401a5f9ad8d283e7c3</citedby><cites>FETCH-LOGICAL-c417t-b70ab204b23d2e2806ff1a46a993db655642d7952391d2a401a5f9ad8d283e7c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-2999(85)90031-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8484522$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3891381$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Morot-Gaudry, Yvette</creatorcontrib><creatorcontrib>Romo, Ranulfo</creatorcontrib><creatorcontrib>Lesbats, Bernard</creatorcontrib><creatorcontrib>Chéramy, André</creatorcontrib><creatorcontrib>Godeheu, Gérard</creatorcontrib><creatorcontrib>Glowinski, Jacques</creatorcontrib><creatorcontrib>Israël, Maurice</creatorcontrib><title>Acetylcholine release in the cat caudate nucleus measured with the choline oxidase method</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>A chemiluminescent assay for the estimation of acetylcholine (ACh) was used to measure ACh release in caudate nuclei (CN) of halothane-anaesthetized cats implanted with push-pull cannulae. The validity of the entire experimental approach used was shown by the fact that ACh release was calcium-dependent and was increased by depolarizing agents (potassium ions, veratridine) as well as by atropine. The effects of GABA (10
−5 M, 30 min) unilateral application into the ventralis medialis and ventralis lateralis thalamic nuclei on ACh release in both CN were then examined. This treatment, known to increase DA release bilaterally, decreased ACh release in both CN. These data further reveal the role of thalamic nuclei in the bilateral regulation of the activity of neurons identified within the basal ganglia and are discussed in the light of the well-known inhibitory influence of nigrostriatal DA neurons on striatal cholinergic neurons.</description><subject>Acetylcholine - metabolism</subject><subject>Acetylcholine release</subject><subject>Alcohol Oxidoreductases</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cats</subject><subject>Caudate nucleus</subject><subject>Caudate Nucleus - metabolism</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Chemiluminescent assay</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gamma-Aminobutyric Acid - pharmacology</subject><subject>In Vitro Techniques</subject><subject>Luminescent Measurements</subject><subject>Male</subject><subject>Methods</subject><subject>Push-pull cannula</subject><subject>Thalamus</subject><subject>Thalamus - drug effects</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1rFTEUhoNU6rX6DyrMooguRnPyMUk2Qil-QcFNu-gqZJIz3JT5qElG7b93pne4S10czuI878vhIeQc6Aeg0HykFETNjDHvtHxvKOVQq2dkB1qZmipgJ2R3RF6QlznfU0qlYfKUnHJtgGvYkbtLj-Wx9_upjyNWCXt0Gas4VmWPlXdlmTm4gtU4-x7nXA0LMCcM1e9Y9gdqC09_YljDA5b9FF6R553rM77e9hm5_fL55upbff3j6_ery-vaC1ClbhV1LaOiZTwwZJo2XQdONM4YHtpGykawoIxk3EBgTlBwsjMu6MA0R-X5GXl76H1I088Zc7FDzB773o04zdmqBhoORvwXBAFGUaEWUBxAn6acE3b2IcXBpUcL1K7q7erVrl6tlvZJvV1jb7b-uR0wHEOb6-V-sd1d9q7vkht9zEdMCy0kYwv26YDhIu1XxGSzjzh6DDGhLzZM8d9__AUTm58P</recordid><startdate>19850326</startdate><enddate>19850326</enddate><creator>Morot-Gaudry, Yvette</creator><creator>Romo, Ranulfo</creator><creator>Lesbats, Bernard</creator><creator>Chéramy, André</creator><creator>Godeheu, Gérard</creator><creator>Glowinski, Jacques</creator><creator>Israël, Maurice</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19850326</creationdate><title>Acetylcholine release in the cat caudate nucleus measured with the choline oxidase method</title><author>Morot-Gaudry, Yvette ; Romo, Ranulfo ; Lesbats, Bernard ; Chéramy, André ; Godeheu, Gérard ; Glowinski, Jacques ; Israël, Maurice</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-b70ab204b23d2e2806ff1a46a993db655642d7952391d2a401a5f9ad8d283e7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Acetylcholine - metabolism</topic><topic>Acetylcholine release</topic><topic>Alcohol Oxidoreductases</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cats</topic><topic>Caudate nucleus</topic><topic>Caudate Nucleus - metabolism</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Chemiluminescent assay</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gamma-Aminobutyric Acid - pharmacology</topic><topic>In Vitro Techniques</topic><topic>Luminescent Measurements</topic><topic>Male</topic><topic>Methods</topic><topic>Push-pull cannula</topic><topic>Thalamus</topic><topic>Thalamus - drug effects</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Morot-Gaudry, Yvette</creatorcontrib><creatorcontrib>Romo, Ranulfo</creatorcontrib><creatorcontrib>Lesbats, Bernard</creatorcontrib><creatorcontrib>Chéramy, André</creatorcontrib><creatorcontrib>Godeheu, Gérard</creatorcontrib><creatorcontrib>Glowinski, Jacques</creatorcontrib><creatorcontrib>Israël, Maurice</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Morot-Gaudry, Yvette</au><au>Romo, Ranulfo</au><au>Lesbats, Bernard</au><au>Chéramy, André</au><au>Godeheu, Gérard</au><au>Glowinski, Jacques</au><au>Israël, Maurice</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Acetylcholine release in the cat caudate nucleus measured with the choline oxidase method</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1985-03-26</date><risdate>1985</risdate><volume>110</volume><issue>1</issue><spage>81</spage><epage>87</epage><pages>81-87</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>A chemiluminescent assay for the estimation of acetylcholine (ACh) was used to measure ACh release in caudate nuclei (CN) of halothane-anaesthetized cats implanted with push-pull cannulae. The validity of the entire experimental approach used was shown by the fact that ACh release was calcium-dependent and was increased by depolarizing agents (potassium ions, veratridine) as well as by atropine. The effects of GABA (10
−5 M, 30 min) unilateral application into the ventralis medialis and ventralis lateralis thalamic nuclei on ACh release in both CN were then examined. This treatment, known to increase DA release bilaterally, decreased ACh release in both CN. These data further reveal the role of thalamic nuclei in the bilateral regulation of the activity of neurons identified within the basal ganglia and are discussed in the light of the well-known inhibitory influence of nigrostriatal DA neurons on striatal cholinergic neurons.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3891381</pmid><doi>10.1016/0014-2999(85)90031-7</doi><tpages>7</tpages></addata></record> |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acetylcholine - metabolism Acetylcholine release Alcohol Oxidoreductases Animals Biological and medical sciences Cats Caudate nucleus Caudate Nucleus - metabolism Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Chemiluminescent assay Female Fundamental and applied biological sciences. Psychology gamma-Aminobutyric Acid - pharmacology In Vitro Techniques Luminescent Measurements Male Methods Push-pull cannula Thalamus Thalamus - drug effects Vertebrates: nervous system and sense organs |
| title | Acetylcholine release in the cat caudate nucleus measured with the choline oxidase method |
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