Characterization of a sperm-specific monoclonal antibody and isolation of 95-kilodalton fertilization antigen-2 from human sperm
Monoclonal antibodies (mAbs) were raised in mice against human sperm. Of the eight hybridomas secreting mAbs that react with human sperm, one, the Vic-1 antibody, was selected for detailed analysis because of its high degree of tissue specificity. The Vic-1 antibody was of the IgG1 subclass and demo...
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| Veröffentlicht in: | Biology of reproduction 1993-12, Vol.49 (6), p.1236-1244 |
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| container_title | Biology of reproduction |
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| creator | Naz, R K Morte, C Garcia-Framis, V Kaplan, P Martinez, P |
| description | Monoclonal antibodies (mAbs) were raised in mice against human sperm. Of the eight hybridomas secreting mAbs that react with
human sperm, one, the Vic-1 antibody, was selected for detailed analysis because of its high degree of tissue specificity.
The Vic-1 antibody was of the IgG1 subclass and demonstrated binding predominantly with the acrosomal regions of viable but
not methanol-fixed noncapacitated and capacitated human sperm cells. It also reacted with the acrosomal and mid-piece regions
of viable capacitated as well as noncapacitated murine sperm, but not with methanol-fixed murine sperm. The Vic-1 antibody
was germ-cell specific as it did not react with any human somatic cell, tissue, or secretion examined including seminal plasma.
The Vic-1 antibody significantly (p = 0.0006) inhibited human sperm penetration of zona-free hamster oocytes in a concentration-dependent
manner; at 15 g% concentration it almost completely blocked sperm penetration. The antibody significantly reduced the acrosome
reaction and the release of acrosin activity in human sperm cells. There was no effect of the Vic-1 antibody on percentage
of motile sperm, although it significantly affected motility characteristics such as linearity, amplitude of lateral head
displacement, and beat frequency; motility parameters involved in the hyperactivation phenomenon related to capacitation and
the acrosome reaction. The Vic-1 antibody recognized a predominant antigen of 95 kDa, designated fertilization antigen-2 (FA-2),
in Western blot and immunoprecipitation procedures using human sperm preparations. The FA-2 antigen was isolated from human
sperm preparations by using an immunoaffinity column containing the Vic-1 antibody. |
| doi_str_mv | 10.1095/biolreprod49.6.1236 |
| format | Article |
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human sperm, one, the Vic-1 antibody, was selected for detailed analysis because of its high degree of tissue specificity.
The Vic-1 antibody was of the IgG1 subclass and demonstrated binding predominantly with the acrosomal regions of viable but
not methanol-fixed noncapacitated and capacitated human sperm cells. It also reacted with the acrosomal and mid-piece regions
of viable capacitated as well as noncapacitated murine sperm, but not with methanol-fixed murine sperm. The Vic-1 antibody
was germ-cell specific as it did not react with any human somatic cell, tissue, or secretion examined including seminal plasma.
The Vic-1 antibody significantly (p = 0.0006) inhibited human sperm penetration of zona-free hamster oocytes in a concentration-dependent
manner; at 15 g% concentration it almost completely blocked sperm penetration. The antibody significantly reduced the acrosome
reaction and the release of acrosin activity in human sperm cells. There was no effect of the Vic-1 antibody on percentage
of motile sperm, although it significantly affected motility characteristics such as linearity, amplitude of lateral head
displacement, and beat frequency; motility parameters involved in the hyperactivation phenomenon related to capacitation and
the acrosome reaction. The Vic-1 antibody recognized a predominant antigen of 95 kDa, designated fertilization antigen-2 (FA-2),
in Western blot and immunoprecipitation procedures using human sperm preparations. The FA-2 antigen was isolated from human
sperm preparations by using an immunoaffinity column containing the Vic-1 antibody.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod49.6.1236</identifier><identifier>PMID: 8286606</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Acrosome - immunology ; Animals ; Antibodies, immunoglobulins ; Antibodies, Monoclonal - biosynthesis ; Antibodies, Monoclonal - immunology ; Antigens - isolation & purification ; Biological and medical sciences ; Blotting, Western ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; Hybridomas - immunology ; Hydrogen-Ion Concentration ; Immunoglobulin G - immunology ; Immunosorbent Techniques ; Male ; Mice ; Mice, Inbred BALB C ; Molecular immunology ; Molecular Weight ; Monoclonal antibodies ; Spermatozoa - immunology</subject><ispartof>Biology of reproduction, 1993-12, Vol.49 (6), p.1236-1244</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3916882$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8286606$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Naz, R K</creatorcontrib><creatorcontrib>Morte, C</creatorcontrib><creatorcontrib>Garcia-Framis, V</creatorcontrib><creatorcontrib>Kaplan, P</creatorcontrib><creatorcontrib>Martinez, P</creatorcontrib><title>Characterization of a sperm-specific monoclonal antibody and isolation of 95-kilodalton fertilization antigen-2 from human sperm</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>Monoclonal antibodies (mAbs) were raised in mice against human sperm. Of the eight hybridomas secreting mAbs that react with
human sperm, one, the Vic-1 antibody, was selected for detailed analysis because of its high degree of tissue specificity.
The Vic-1 antibody was of the IgG1 subclass and demonstrated binding predominantly with the acrosomal regions of viable but
not methanol-fixed noncapacitated and capacitated human sperm cells. It also reacted with the acrosomal and mid-piece regions
of viable capacitated as well as noncapacitated murine sperm, but not with methanol-fixed murine sperm. The Vic-1 antibody
was germ-cell specific as it did not react with any human somatic cell, tissue, or secretion examined including seminal plasma.
The Vic-1 antibody significantly (p = 0.0006) inhibited human sperm penetration of zona-free hamster oocytes in a concentration-dependent
manner; at 15 g% concentration it almost completely blocked sperm penetration. The antibody significantly reduced the acrosome
reaction and the release of acrosin activity in human sperm cells. There was no effect of the Vic-1 antibody on percentage
of motile sperm, although it significantly affected motility characteristics such as linearity, amplitude of lateral head
displacement, and beat frequency; motility parameters involved in the hyperactivation phenomenon related to capacitation and
the acrosome reaction. The Vic-1 antibody recognized a predominant antigen of 95 kDa, designated fertilization antigen-2 (FA-2),
in Western blot and immunoprecipitation procedures using human sperm preparations. The FA-2 antigen was isolated from human
sperm preparations by using an immunoaffinity column containing the Vic-1 antibody.</description><subject>Acrosome - immunology</subject><subject>Animals</subject><subject>Antibodies, immunoglobulins</subject><subject>Antibodies, Monoclonal - biosynthesis</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens - isolation & purification</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Hybridomas - immunology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunosorbent Techniques</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Molecular immunology</subject><subject>Molecular Weight</subject><subject>Monoclonal antibodies</subject><subject>Spermatozoa - immunology</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFUctKxDAUDaLo-PgCEboQdx3zbrKUwRcIbnRdbtPURtNmTDoM48pPN-Kom_s851w4F6FTgucEa3HZuOCjXcbQcj2Xc0KZ3EEzIqguKyrVLpphjGXJmGQH6DClV4wJZ5Tto31FlZRYztDnoocIZrLRfcDkwliEroAiLW0cyhyN65wphjAG48MIvoBxck1oN7loC5eC_2NpUb45H1rwUx50Nk7O_4p-s17sWNKii2Eo-tUA48-RY7TXgU_2ZJuP0PPN9dPirnx4vL1fXD2UPZVyKhtBiQUtTMdZKzqeG4JtpRtVtRIaDrghjTKqAiGoYNxyroVQFHNWgdSGHaGLH93s1_vKpqkeXDLWexhtWKW6kkSobFsGnm2Bq2awbb2MboC4qbeW5f35dg_JgO8ijMalPxjTRCpF_-_17qVfu2jrNID3WZTV6_Wa61rW3x9jX-dEjD0</recordid><startdate>19931201</startdate><enddate>19931201</enddate><creator>Naz, R K</creator><creator>Morte, C</creator><creator>Garcia-Framis, V</creator><creator>Kaplan, P</creator><creator>Martinez, P</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19931201</creationdate><title>Characterization of a sperm-specific monoclonal antibody and isolation of 95-kilodalton fertilization antigen-2 from human sperm</title><author>Naz, R K ; Morte, C ; Garcia-Framis, V ; Kaplan, P ; Martinez, P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h266t-b521ea95cf43d5f41ea10e79b87d6ab4a0b1b8c87a552534e44955820437a69c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Acrosome - immunology</topic><topic>Animals</topic><topic>Antibodies, immunoglobulins</topic><topic>Antibodies, Monoclonal - biosynthesis</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antigens - isolation & purification</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Hybridomas - immunology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Immunoglobulin G - immunology</topic><topic>Immunosorbent Techniques</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Molecular immunology</topic><topic>Molecular Weight</topic><topic>Monoclonal antibodies</topic><topic>Spermatozoa - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Naz, R K</creatorcontrib><creatorcontrib>Morte, C</creatorcontrib><creatorcontrib>Garcia-Framis, V</creatorcontrib><creatorcontrib>Kaplan, P</creatorcontrib><creatorcontrib>Martinez, P</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Naz, R K</au><au>Morte, C</au><au>Garcia-Framis, V</au><au>Kaplan, P</au><au>Martinez, P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a sperm-specific monoclonal antibody and isolation of 95-kilodalton fertilization antigen-2 from human sperm</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1993-12-01</date><risdate>1993</risdate><volume>49</volume><issue>6</issue><spage>1236</spage><epage>1244</epage><pages>1236-1244</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>Monoclonal antibodies (mAbs) were raised in mice against human sperm. Of the eight hybridomas secreting mAbs that react with
human sperm, one, the Vic-1 antibody, was selected for detailed analysis because of its high degree of tissue specificity.
The Vic-1 antibody was of the IgG1 subclass and demonstrated binding predominantly with the acrosomal regions of viable but
not methanol-fixed noncapacitated and capacitated human sperm cells. It also reacted with the acrosomal and mid-piece regions
of viable capacitated as well as noncapacitated murine sperm, but not with methanol-fixed murine sperm. The Vic-1 antibody
was germ-cell specific as it did not react with any human somatic cell, tissue, or secretion examined including seminal plasma.
The Vic-1 antibody significantly (p = 0.0006) inhibited human sperm penetration of zona-free hamster oocytes in a concentration-dependent
manner; at 15 g% concentration it almost completely blocked sperm penetration. The antibody significantly reduced the acrosome
reaction and the release of acrosin activity in human sperm cells. There was no effect of the Vic-1 antibody on percentage
of motile sperm, although it significantly affected motility characteristics such as linearity, amplitude of lateral head
displacement, and beat frequency; motility parameters involved in the hyperactivation phenomenon related to capacitation and
the acrosome reaction. The Vic-1 antibody recognized a predominant antigen of 95 kDa, designated fertilization antigen-2 (FA-2),
in Western blot and immunoprecipitation procedures using human sperm preparations. The FA-2 antigen was isolated from human
sperm preparations by using an immunoaffinity column containing the Vic-1 antibody.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>8286606</pmid><doi>10.1095/biolreprod49.6.1236</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3363 |
| ispartof | Biology of reproduction, 1993-12, Vol.49 (6), p.1236-1244 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76158726 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Acrosome - immunology Animals Antibodies, immunoglobulins Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antigens - isolation & purification Biological and medical sciences Blotting, Western Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Hybridomas - immunology Hydrogen-Ion Concentration Immunoglobulin G - immunology Immunosorbent Techniques Male Mice Mice, Inbred BALB C Molecular immunology Molecular Weight Monoclonal antibodies Spermatozoa - immunology |
| title | Characterization of a sperm-specific monoclonal antibody and isolation of 95-kilodalton fertilization antigen-2 from human sperm |
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