Assessment of ram sperm mitochondrial function by quantitative determination of sperm rhodamine 123 accumulation

A simple procedure is described for determining the functional state of ram sperm mitochondria by quantitative measurement of sperm rhodamine 123 (R 123) accumulation. Sperm were incubated with 1 μg/ml R 123, and the accumulated R 123 was measured fluorimetrically after release from washed sperm by...

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Veröffentlicht in:	Molecular reproduction and development 1993-11, Vol.36 (3), p.354-360
Hauptverfasser:	Windsor, D.P, White, I.G
Format:	Artikel
Sprache:	eng
Schlagworte:	2,4-Dinitrophenol Animals BELIER Deoxyglucose - metabolism Detergents - pharmacology Dinitrophenols - pharmacology ESPERMATOZOO Fluorescence Fluorometry In Vitro Techniques Male Malonates - pharmacology Membrane potential Membrane Potentials Microscopy, Fluorescence MITOCHONDRIA Mitochondria - metabolism MITOCHONDRIE MITOCONDRIA MORUECO Oxygen Consumption - drug effects RAMS Respiration Rhodamine 123 Rhodamines - metabolism Sheep SPERMATOZOA Spermatozoa - drug effects Spermatozoa - metabolism SPERMATOZOIDE Triton X-100
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