An enzyme-linked immunosorbent assay (ELISA) for monitoring guineapigs and rabbits for Bordetella bronchiseptica antibodies
An enzyme-linked immunosorbent assay (ELISA) for monitoring antibodies specific to Bordetella bronchiseptica in guineapigs and rabbits was developed. In conventional and SPF colonies of guineapigs and rabbits, the ELISA was equally successful in detecting infected animals when compared to selective...
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| Veröffentlicht in: | Laboratory animals (London) 1993-01, Vol.27 (4), p.342-349 |
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| container_title | Laboratory animals (London) |
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| creator | Boot, R Bakker, R H Thuis, H Veenema, J L |
| description | An enzyme-linked immunosorbent assay (ELISA) for monitoring antibodies specific to Bordetella bronchiseptica in guineapigs and rabbits was developed. In conventional and SPF colonies of guineapigs and rabbits, the ELISA was equally successful in detecting infected animals when compared to selective cultivation from the respiratory tract. The ELISA showed a sensitivity of 100% and a specificity of 90% in guineapigs. In rabbits the sensitivity and specificity of the ELISA were 97% and 91% respectively. In rabbit sera from infected colonies, ELISA activity showed a statistically significant correlation with titres obtained in the micro-agglutination test. Since serologically unrelated strains of the bacterium exist, the monitoring of animals for B. bronchiseptica infection by ELISA should be performed with various antigens. |
| doi_str_mv | 10.1258/002367793780745624 |
| format | Article |
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In conventional and SPF colonies of guineapigs and rabbits, the ELISA was equally successful in detecting infected animals when compared to selective cultivation from the respiratory tract. The ELISA showed a sensitivity of 100% and a specificity of 90% in guineapigs. In rabbits the sensitivity and specificity of the ELISA were 97% and 91% respectively. In rabbit sera from infected colonies, ELISA activity showed a statistically significant correlation with titres obtained in the micro-agglutination test. Since serologically unrelated strains of the bacterium exist, the monitoring of animals for B. bronchiseptica infection by ELISA should be performed with various antigens.</description><identifier>ISSN: 0023-6772</identifier><identifier>EISSN: 1758-1117</identifier><identifier>DOI: 10.1258/002367793780745624</identifier><identifier>PMID: 8277707</identifier><language>eng</language><publisher>London, England: SAGE Publications</publisher><subject>Animals ; Antibodies, Bacterial - blood ; Antibody Specificity ; Bordetella bronchiseptica - immunology ; Bordetella Infections - microbiology ; Bordetella Infections - veterinary ; Cross Reactions ; Enzyme-Linked Immunosorbent Assay - veterinary ; Female ; Guinea Pigs ; Rabbits ; Rats ; Sensitivity and Specificity ; Species Specificity</subject><ispartof>Laboratory animals (London), 1993-01, Vol.27 (4), p.342-349</ispartof><rights>1993 Royal Society of Medicine Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2974-20ba0309fcd1eb5c13bc9366c8998a7bb3b43e9f0eff175b9b2ae0d6a7e8453d3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8277707$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Boot, R</creatorcontrib><creatorcontrib>Bakker, R H</creatorcontrib><creatorcontrib>Thuis, H</creatorcontrib><creatorcontrib>Veenema, J L</creatorcontrib><title>An enzyme-linked immunosorbent assay (ELISA) for monitoring guineapigs and rabbits for Bordetella bronchiseptica antibodies</title><title>Laboratory animals (London)</title><addtitle>Lab Anim</addtitle><description>An enzyme-linked immunosorbent assay (ELISA) for monitoring antibodies specific to Bordetella bronchiseptica in guineapigs and rabbits was developed. In conventional and SPF colonies of guineapigs and rabbits, the ELISA was equally successful in detecting infected animals when compared to selective cultivation from the respiratory tract. The ELISA showed a sensitivity of 100% and a specificity of 90% in guineapigs. In rabbits the sensitivity and specificity of the ELISA were 97% and 91% respectively. In rabbit sera from infected colonies, ELISA activity showed a statistically significant correlation with titres obtained in the micro-agglutination test. Since serologically unrelated strains of the bacterium exist, the monitoring of animals for B. bronchiseptica infection by ELISA should be performed with various antigens.</description><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>Antibody Specificity</subject><subject>Bordetella bronchiseptica - immunology</subject><subject>Bordetella Infections - microbiology</subject><subject>Bordetella Infections - veterinary</subject><subject>Cross Reactions</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Female</subject><subject>Guinea Pigs</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Sensitivity and Specificity</subject><subject>Species Specificity</subject><issn>0023-6772</issn><issn>1758-1117</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtPwzAQhC0EKqXwB5CQfEJwCPUjiZNjqcpDqsQBOEe2sykujV3s5FD487i04oLEabXab0Y7g9A5JTeUZcWYEMZzIUouCiLSLGfpARpSkRUJpVQcouEWSCLBjtFJCMu40rQgAzQomBCCiCH6mlgM9nPTQrIy9h1qbNq2ty44r8B2WIYgN_hqNn98nlzjxnncOms6541d4EVvLMi1WQQsbY29VMp04Ye6db6GDlYriZV3Vr-ZAOvOaBnJzihXGwin6KiRqwBn-zlCr3ezl-lDMn-6f5xO5olmpUgTRpQknJSNrimoTFOudMnzXBdlWUihFFcph7Ih0DQxvCoVk0DqXAoo0ozXfIQud75r7z56CF3VmqC3v1lwfahEHltJ8zyCbAdq70Lw0FRrb1rpNxUl1bbx6m_jUXSxd-9VC_WvZF9xvI939yAXUC1d720M-5_jN6nri0U</recordid><startdate>19930101</startdate><enddate>19930101</enddate><creator>Boot, R</creator><creator>Bakker, R H</creator><creator>Thuis, H</creator><creator>Veenema, J L</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930101</creationdate><title>An enzyme-linked immunosorbent assay (ELISA) for monitoring guineapigs and rabbits for Bordetella bronchiseptica antibodies</title><author>Boot, R ; Bakker, R H ; Thuis, H ; Veenema, J L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2974-20ba0309fcd1eb5c13bc9366c8998a7bb3b43e9f0eff175b9b2ae0d6a7e8453d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>Antibody Specificity</topic><topic>Bordetella bronchiseptica - immunology</topic><topic>Bordetella Infections - microbiology</topic><topic>Bordetella Infections - veterinary</topic><topic>Cross Reactions</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Female</topic><topic>Guinea Pigs</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Sensitivity and Specificity</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Boot, R</creatorcontrib><creatorcontrib>Bakker, R H</creatorcontrib><creatorcontrib>Thuis, H</creatorcontrib><creatorcontrib>Veenema, J L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Laboratory animals (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Boot, R</au><au>Bakker, R H</au><au>Thuis, H</au><au>Veenema, J L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An enzyme-linked immunosorbent assay (ELISA) for monitoring guineapigs and rabbits for Bordetella bronchiseptica antibodies</atitle><jtitle>Laboratory animals (London)</jtitle><addtitle>Lab Anim</addtitle><date>1993-01-01</date><risdate>1993</risdate><volume>27</volume><issue>4</issue><spage>342</spage><epage>349</epage><pages>342-349</pages><issn>0023-6772</issn><eissn>1758-1117</eissn><abstract>An enzyme-linked immunosorbent assay (ELISA) for monitoring antibodies specific to Bordetella bronchiseptica in guineapigs and rabbits was developed. In conventional and SPF colonies of guineapigs and rabbits, the ELISA was equally successful in detecting infected animals when compared to selective cultivation from the respiratory tract. The ELISA showed a sensitivity of 100% and a specificity of 90% in guineapigs. In rabbits the sensitivity and specificity of the ELISA were 97% and 91% respectively. In rabbit sera from infected colonies, ELISA activity showed a statistically significant correlation with titres obtained in the micro-agglutination test. Since serologically unrelated strains of the bacterium exist, the monitoring of animals for B. bronchiseptica infection by ELISA should be performed with various antigens.</abstract><cop>London, England</cop><pub>SAGE Publications</pub><pmid>8277707</pmid><doi>10.1258/002367793780745624</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0023-6772 |
| ispartof | Laboratory animals (London), 1993-01, Vol.27 (4), p.342-349 |
| issn | 0023-6772 1758-1117 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Antibodies, Bacterial - blood Antibody Specificity Bordetella bronchiseptica - immunology Bordetella Infections - microbiology Bordetella Infections - veterinary Cross Reactions Enzyme-Linked Immunosorbent Assay - veterinary Female Guinea Pigs Rabbits Rats Sensitivity and Specificity Species Specificity |
| title | An enzyme-linked immunosorbent assay (ELISA) for monitoring guineapigs and rabbits for Bordetella bronchiseptica antibodies |
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