The Role of Citrate in the Regulation of Hepatic Fatty Acid Synthesis by Insulin and Glucagon
Summary Incubation of intact rat hepatocytes with insulin and glucagon resulted in increased and decreased rates of DE NOVO fatty acid biosynthesis, respectively. These changes were paralleled by corresponding alterations in the activity of acetyl-CoA carboxylase, an important regulatory enzyme of t...
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| Veröffentlicht in: | Hormone and metabolic research 1993-10, Vol.25 (10), p.525-527 |
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| container_title | Hormone and metabolic research |
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| creator | Geelen, M. J. H. Schmitz, Marion G. J. |
| description | Summary
Incubation of intact rat hepatocytes with insulin and glucagon resulted in increased and decreased rates of DE NOVO fatty acid biosynthesis, respectively. These changes were paralleled by corresponding alterations in the activity of acetyl-CoA carboxylase, an important regulatory enzyme of this pathway. The hormonal conditions imposed on the hepatocytes did not change the cellular or the cytosolic level of citrate. Incubation of hepatocytes with octanoate showed a parallel increase in the rate of fatty acid synthesis and acetyl-CoA carboxylase with a concomitant elevation of the cellular citrate level. The increase in whole-cell citrate was mainly due to a marked increase in the level of cytosolic citrate. Collectively, our data indicate that insulin and glucagon-determined changes in acetyl-CoA carboxylase are not mediated by changes in cytosolic citrate levels. |
| doi_str_mv | 10.1055/s-2007-1002166 |
| format | Article |
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Incubation of intact rat hepatocytes with insulin and glucagon resulted in increased and decreased rates of DE NOVO fatty acid biosynthesis, respectively. These changes were paralleled by corresponding alterations in the activity of acetyl-CoA carboxylase, an important regulatory enzyme of this pathway. The hormonal conditions imposed on the hepatocytes did not change the cellular or the cytosolic level of citrate. Incubation of hepatocytes with octanoate showed a parallel increase in the rate of fatty acid synthesis and acetyl-CoA carboxylase with a concomitant elevation of the cellular citrate level. The increase in whole-cell citrate was mainly due to a marked increase in the level of cytosolic citrate. Collectively, our data indicate that insulin and glucagon-determined changes in acetyl-CoA carboxylase are not mediated by changes in cytosolic citrate levels.</description><identifier>ISSN: 0018-5043</identifier><identifier>EISSN: 1439-4286</identifier><identifier>DOI: 10.1055/s-2007-1002166</identifier><identifier>PMID: 7903266</identifier><identifier>CODEN: HMMRA2</identifier><language>eng</language><publisher>Stuttgart: Thieme</publisher><subject>Acetyl-CoA Carboxylase - metabolism ; Animals ; Biological and medical sciences ; Caprylates - pharmacology ; Citrates - metabolism ; Citric Acid ; Cytosol - metabolism ; Enzyme Activation ; Fatty Acids - biosynthesis ; Fundamental and applied biological sciences. Psychology ; Glucagon - pharmacology ; Insulin - pharmacology ; Lipids. Glycolipids ; Liver - drug effects ; Liver - metabolism ; Liver - ultrastructure ; Male ; Metabolisms and neurohumoral controls ; Originals Basic ; Rats ; Rats, Wistar ; Subcellular Fractions - metabolism ; Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><ispartof>Hormone and metabolic research, 1993-10, Vol.25 (10), p.525-527</ispartof><rights>Georg Thieme Verlag, Stuttgart · New York</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-166313eb79a0450a7249ddd55c6fac616c9041f493bdf80c62642f120b7c9c343</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.thieme-connect.de/products/ejournals/pdf/10.1055/s-2007-1002166.pdf$$EPDF$$P50$$Gthieme$$H</linktopdf><link.rule.ids>314,776,780,3004,3005,27901,27902,54534</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3750148$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7903266$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Geelen, M. J. H.</creatorcontrib><creatorcontrib>Schmitz, Marion G. J.</creatorcontrib><title>The Role of Citrate in the Regulation of Hepatic Fatty Acid Synthesis by Insulin and Glucagon</title><title>Hormone and metabolic research</title><addtitle>Horm Metab Res</addtitle><description>Summary
Incubation of intact rat hepatocytes with insulin and glucagon resulted in increased and decreased rates of DE NOVO fatty acid biosynthesis, respectively. These changes were paralleled by corresponding alterations in the activity of acetyl-CoA carboxylase, an important regulatory enzyme of this pathway. The hormonal conditions imposed on the hepatocytes did not change the cellular or the cytosolic level of citrate. Incubation of hepatocytes with octanoate showed a parallel increase in the rate of fatty acid synthesis and acetyl-CoA carboxylase with a concomitant elevation of the cellular citrate level. The increase in whole-cell citrate was mainly due to a marked increase in the level of cytosolic citrate. Collectively, our data indicate that insulin and glucagon-determined changes in acetyl-CoA carboxylase are not mediated by changes in cytosolic citrate levels.</description><subject>Acetyl-CoA Carboxylase - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Caprylates - pharmacology</subject><subject>Citrates - metabolism</subject><subject>Citric Acid</subject><subject>Cytosol - metabolism</subject><subject>Enzyme Activation</subject><subject>Fatty Acids - biosynthesis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucagon - pharmacology</subject><subject>Insulin - pharmacology</subject><subject>Lipids. Glycolipids</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Liver - ultrastructure</subject><subject>Male</subject><subject>Metabolisms and neurohumoral controls</subject><subject>Originals Basic</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Subcellular Fractions - metabolism</subject><subject>Vertebrates: anatomy and physiology, studies on body, several organs or systems</subject><issn>0018-5043</issn><issn>1439-4286</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1LwzAchoMoc06v3oQcxFtnvpq2xzHcJgwEnUcJaZpuGW06m_TQ_96Uld085eN9fm_CA8AjRnOM4vjVRQShJMIIEcz5FZhiRrOIkZRfgylCOI1ixOgtuHPuGI4sw2wCJkmGKOF8Cn52Bw0_m0rDpoRL41vpNTQW-uFa77tKetPYIdzoU9gruJLe93ChTAG_ehs4ZxzMe_huXVeFSWkLuK46JfeNvQc3paycfhjXGfheve2Wm2j7sX5fLraRYoT6KHycYqrzJJOIxUgmhGVFUcSx4qVUHHOVIYZLltG8KFOkOOGMlJigPFGZoozOwMu599Q2v512XtTGKV1V0uqmcyLhwUooDeD8DKq2ca7VpTi1ppZtLzASg0_hxOBTjD7DwNPY3OW1Li74KDDkz2MunZJV2UqrjLtgNImD8zRg0RnzB6NrLY5N19pg5L9n_wBni4kx</recordid><startdate>19931001</startdate><enddate>19931001</enddate><creator>Geelen, M. J. H.</creator><creator>Schmitz, Marion G. J.</creator><general>Thieme</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19931001</creationdate><title>The Role of Citrate in the Regulation of Hepatic Fatty Acid Synthesis by Insulin and Glucagon</title><author>Geelen, M. J. H. ; Schmitz, Marion G. J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-166313eb79a0450a7249ddd55c6fac616c9041f493bdf80c62642f120b7c9c343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Acetyl-CoA Carboxylase - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Caprylates - pharmacology</topic><topic>Citrates - metabolism</topic><topic>Citric Acid</topic><topic>Cytosol - metabolism</topic><topic>Enzyme Activation</topic><topic>Fatty Acids - biosynthesis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucagon - pharmacology</topic><topic>Insulin - pharmacology</topic><topic>Lipids. Glycolipids</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Liver - ultrastructure</topic><topic>Male</topic><topic>Metabolisms and neurohumoral controls</topic><topic>Originals Basic</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Subcellular Fractions - metabolism</topic><topic>Vertebrates: anatomy and physiology, studies on body, several organs or systems</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Geelen, M. J. H.</creatorcontrib><creatorcontrib>Schmitz, Marion G. J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Hormone and metabolic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Geelen, M. J. H.</au><au>Schmitz, Marion G. J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Role of Citrate in the Regulation of Hepatic Fatty Acid Synthesis by Insulin and Glucagon</atitle><jtitle>Hormone and metabolic research</jtitle><addtitle>Horm Metab Res</addtitle><date>1993-10-01</date><risdate>1993</risdate><volume>25</volume><issue>10</issue><spage>525</spage><epage>527</epage><pages>525-527</pages><issn>0018-5043</issn><eissn>1439-4286</eissn><coden>HMMRA2</coden><abstract>Summary
Incubation of intact rat hepatocytes with insulin and glucagon resulted in increased and decreased rates of DE NOVO fatty acid biosynthesis, respectively. These changes were paralleled by corresponding alterations in the activity of acetyl-CoA carboxylase, an important regulatory enzyme of this pathway. The hormonal conditions imposed on the hepatocytes did not change the cellular or the cytosolic level of citrate. Incubation of hepatocytes with octanoate showed a parallel increase in the rate of fatty acid synthesis and acetyl-CoA carboxylase with a concomitant elevation of the cellular citrate level. The increase in whole-cell citrate was mainly due to a marked increase in the level of cytosolic citrate. Collectively, our data indicate that insulin and glucagon-determined changes in acetyl-CoA carboxylase are not mediated by changes in cytosolic citrate levels.</abstract><cop>Stuttgart</cop><cop>New York, NY</cop><pub>Thieme</pub><pmid>7903266</pmid><doi>10.1055/s-2007-1002166</doi><tpages>3</tpages></addata></record> |
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| subjects | Acetyl-CoA Carboxylase - metabolism Animals Biological and medical sciences Caprylates - pharmacology Citrates - metabolism Citric Acid Cytosol - metabolism Enzyme Activation Fatty Acids - biosynthesis Fundamental and applied biological sciences. Psychology Glucagon - pharmacology Insulin - pharmacology Lipids. Glycolipids Liver - drug effects Liver - metabolism Liver - ultrastructure Male Metabolisms and neurohumoral controls Originals Basic Rats Rats, Wistar Subcellular Fractions - metabolism Vertebrates: anatomy and physiology, studies on body, several organs or systems |
| title | The Role of Citrate in the Regulation of Hepatic Fatty Acid Synthesis by Insulin and Glucagon |
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