Distribution and Characterization of Neuropeptide FF-like Immunoreactivity in the Rat Nervous System with a Monoclonal Antibody
Monoclonal antibodies against neuropeptide FF were produced and characterized. The antibodies are directed and highly specific to neuropeptide FF, and reactivity requires the C‐terminal dipeptide of neuropeptide FF (Arg‐Phe‐NH2). Tissue extracts from bovine spinal cord, rat spinal cord and hypothala...
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| Veröffentlicht in: | The European journal of neuroscience 1993-10, Vol.5 (10), p.1339-1348 |
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| container_title | The European journal of neuroscience |
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| creator | Lee, Che-Hung Wasowicz, Krzysztof Brown, Robert Majane, Elizabeth A. Yang, Hsiu-Ying T. Panula, Pertti |
| description | Monoclonal antibodies against neuropeptide FF were produced and characterized. The antibodies are directed and highly specific to neuropeptide FF, and reactivity requires the C‐terminal dipeptide of neuropeptide FF (Arg‐Phe‐NH2). Tissue extracts from bovine spinal cord, rat spinal cord and hypothalamus were analysed by high‐pressure liquid chromatography coupled with radioimmunoassay using the characterized monoclonal antibody. Only one immunoreactive peptide was detected and it coeluted with authentic neuropeptide FF. Using this highly specific monoclonal antibody, the distribution of neuropeptide FF‐like immunoreactivity was further studied by indirect immunohistochemistry. Immunoreactivity was seen in two major cell groups in the rat brain. The largest cell group was located in the medial hypothalamus between the dorsomedial and ventromedial nuclei. The other one was found in the nucleus of the solitary tract. Fibres immunoreactive for neuropeptide FF were located in the lateral septal nucleus, amygdala, different hypothalamic areas, nucleus of the solitary tract, ventral medulla, trigeminal complex and the dorsal horn of the spinal cord. Spinal and sympathetic ganglia were non‐reactive. No neuropeptide FF immunoreactivity was seen in the gut autonomic nervous system or endocrine cells. The results show that neuropeptide FF‐like immunoreactivity has a clearly more limited distribution in the nervous system than typical brain–gut peptides. |
| doi_str_mv | 10.1111/j.1460-9568.1993.tb00920.x |
| format | Article |
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The antibodies are directed and highly specific to neuropeptide FF, and reactivity requires the C‐terminal dipeptide of neuropeptide FF (Arg‐Phe‐NH2). Tissue extracts from bovine spinal cord, rat spinal cord and hypothalamus were analysed by high‐pressure liquid chromatography coupled with radioimmunoassay using the characterized monoclonal antibody. Only one immunoreactive peptide was detected and it coeluted with authentic neuropeptide FF. Using this highly specific monoclonal antibody, the distribution of neuropeptide FF‐like immunoreactivity was further studied by indirect immunohistochemistry. Immunoreactivity was seen in two major cell groups in the rat brain. The largest cell group was located in the medial hypothalamus between the dorsomedial and ventromedial nuclei. The other one was found in the nucleus of the solitary tract. Fibres immunoreactive for neuropeptide FF were located in the lateral septal nucleus, amygdala, different hypothalamic areas, nucleus of the solitary tract, ventral medulla, trigeminal complex and the dorsal horn of the spinal cord. Spinal and sympathetic ganglia were non‐reactive. No neuropeptide FF immunoreactivity was seen in the gut autonomic nervous system or endocrine cells. The results show that neuropeptide FF‐like immunoreactivity has a clearly more limited distribution in the nervous system than typical brain–gut peptides.</description><identifier>ISSN: 0953-816X</identifier><identifier>EISSN: 1460-9568</identifier><identifier>DOI: 10.1111/j.1460-9568.1993.tb00920.x</identifier><identifier>PMID: 8275233</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acid Sequence ; analgesia ; Animals ; Antibodies, Monoclonal ; Antibody Specificity ; Biological and medical sciences ; Cattle ; Central nervous system ; Central neurotransmission. Neuromudulation. Pathways and receptors ; Chromatography, High Pressure Liquid ; Dipeptides - analysis ; Enzyme-Linked Immunosorbent Assay ; Fundamental and applied biological sciences. Psychology ; hypothalamus ; Hypothalamus - chemistry ; Hypothalamus - cytology ; Immunohistochemistry ; Male ; Molecular Sequence Data ; morphine ; Nerve Fibers - ultrastructure ; Neurons - cytology ; neuropeptide FF ; Neuropeptides - analysis ; nucleus of the solitary tract ; Oligopeptides - analysis ; Organ Specificity ; Prosencephalon - cytology ; Radioimmunoassay ; Rats ; Rats, Sprague-Dawley ; Spinal Cord - chemistry ; Spinal Cord - cytology ; Vertebrates: nervous system and sense organs</subject><ispartof>The European journal of neuroscience, 1993-10, Vol.5 (10), p.1339-1348</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4689-45cb4443b0b0a9fe5893f3bd8a7ff7610929a53d66936b3338c3be7fe9bbcf593</citedby><cites>FETCH-LOGICAL-c4689-45cb4443b0b0a9fe5893f3bd8a7ff7610929a53d66936b3338c3be7fe9bbcf593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1460-9568.1993.tb00920.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1460-9568.1993.tb00920.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3764088$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8275233$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Che-Hung</creatorcontrib><creatorcontrib>Wasowicz, Krzysztof</creatorcontrib><creatorcontrib>Brown, Robert</creatorcontrib><creatorcontrib>Majane, Elizabeth A.</creatorcontrib><creatorcontrib>Yang, Hsiu-Ying T.</creatorcontrib><creatorcontrib>Panula, Pertti</creatorcontrib><title>Distribution and Characterization of Neuropeptide FF-like Immunoreactivity in the Rat Nervous System with a Monoclonal Antibody</title><title>The European journal of neuroscience</title><addtitle>Eur J Neurosci</addtitle><description>Monoclonal antibodies against neuropeptide FF were produced and characterized. The antibodies are directed and highly specific to neuropeptide FF, and reactivity requires the C‐terminal dipeptide of neuropeptide FF (Arg‐Phe‐NH2). Tissue extracts from bovine spinal cord, rat spinal cord and hypothalamus were analysed by high‐pressure liquid chromatography coupled with radioimmunoassay using the characterized monoclonal antibody. Only one immunoreactive peptide was detected and it coeluted with authentic neuropeptide FF. Using this highly specific monoclonal antibody, the distribution of neuropeptide FF‐like immunoreactivity was further studied by indirect immunohistochemistry. Immunoreactivity was seen in two major cell groups in the rat brain. The largest cell group was located in the medial hypothalamus between the dorsomedial and ventromedial nuclei. The other one was found in the nucleus of the solitary tract. Fibres immunoreactive for neuropeptide FF were located in the lateral septal nucleus, amygdala, different hypothalamic areas, nucleus of the solitary tract, ventral medulla, trigeminal complex and the dorsal horn of the spinal cord. Spinal and sympathetic ganglia were non‐reactive. No neuropeptide FF immunoreactivity was seen in the gut autonomic nervous system or endocrine cells. The results show that neuropeptide FF‐like immunoreactivity has a clearly more limited distribution in the nervous system than typical brain–gut peptides.</description><subject>Amino Acid Sequence</subject><subject>analgesia</subject><subject>Animals</subject><subject>Antibodies, Monoclonal</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Dipeptides - analysis</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hypothalamus</subject><subject>Hypothalamus - chemistry</subject><subject>Hypothalamus - cytology</subject><subject>Immunohistochemistry</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>morphine</subject><subject>Nerve Fibers - ultrastructure</subject><subject>Neurons - cytology</subject><subject>neuropeptide FF</subject><subject>Neuropeptides - analysis</subject><subject>nucleus of the solitary tract</subject><subject>Oligopeptides - analysis</subject><subject>Organ Specificity</subject><subject>Prosencephalon - cytology</subject><subject>Radioimmunoassay</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Spinal Cord - chemistry</subject><subject>Spinal Cord - cytology</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0953-816X</issn><issn>1460-9568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVklFv0zAUhSMEGmXwE5AshPaW4vTGjs0Lmtp1DEaRYIiJF8tOHNVdEhfb2Vpe-Os4NOorwn6w5Pud42sfJ8mrDE-zON5spllOccoJZdOMc5gGhTGf4enuUTI5lh4nE8wJpCyjt0-TZ95vMMaM5uQkOWGzgswAJsnvhfHBGdUHYzskuwrN19LJMmhnfsm_m7ZGK907u9XbYCqNlsu0MXcaXbVt31mnI2zuTdgj06Gw1uiLDFHg7m3v0de9D7pFDyaskUSfbGfLxnayQeddMMpW--fJk1o2Xr8Y19Pk2_LiZv4-vf58eTU_v07LnDKe5qRUeZ6DwgpLXmvCONSgKiaLui5oFm_PJYGKUg5UAQArQemi1lypsiYcTpOzg-_W2Z-99kG0xpe6aWSnY6MieuSQUfgnOMO8IHFG8O0BLJ313ulabJ1ppduLDIshJrERQxZiyEIMMYkxJrGL4pfjKb1qdXWUjrnE-uuxLn0pm9rJrjT-iEFBc8xYxN4dsAfT6P1_NCAuPqwygOFd0oND_AZ6d3SQ7k7QAgoivq8uxUf243ZBFjcCwx_kJMDS</recordid><startdate>199310</startdate><enddate>199310</enddate><creator>Lee, Che-Hung</creator><creator>Wasowicz, Krzysztof</creator><creator>Brown, Robert</creator><creator>Majane, Elizabeth A.</creator><creator>Yang, Hsiu-Ying T.</creator><creator>Panula, Pertti</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>199310</creationdate><title>Distribution and Characterization of Neuropeptide FF-like Immunoreactivity in the Rat Nervous System with a Monoclonal Antibody</title><author>Lee, Che-Hung ; Wasowicz, Krzysztof ; Brown, Robert ; Majane, Elizabeth A. ; Yang, Hsiu-Ying T. ; Panula, Pertti</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4689-45cb4443b0b0a9fe5893f3bd8a7ff7610929a53d66936b3338c3be7fe9bbcf593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Sequence</topic><topic>analgesia</topic><topic>Animals</topic><topic>Antibodies, Monoclonal</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Dipeptides - analysis</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hypothalamus</topic><topic>Hypothalamus - chemistry</topic><topic>Hypothalamus - cytology</topic><topic>Immunohistochemistry</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>morphine</topic><topic>Nerve Fibers - ultrastructure</topic><topic>Neurons - cytology</topic><topic>neuropeptide FF</topic><topic>Neuropeptides - analysis</topic><topic>nucleus of the solitary tract</topic><topic>Oligopeptides - analysis</topic><topic>Organ Specificity</topic><topic>Prosencephalon - cytology</topic><topic>Radioimmunoassay</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Spinal Cord - chemistry</topic><topic>Spinal Cord - cytology</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Che-Hung</creatorcontrib><creatorcontrib>Wasowicz, Krzysztof</creatorcontrib><creatorcontrib>Brown, Robert</creatorcontrib><creatorcontrib>Majane, Elizabeth A.</creatorcontrib><creatorcontrib>Yang, Hsiu-Ying T.</creatorcontrib><creatorcontrib>Panula, Pertti</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The European journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Che-Hung</au><au>Wasowicz, Krzysztof</au><au>Brown, Robert</au><au>Majane, Elizabeth A.</au><au>Yang, Hsiu-Ying T.</au><au>Panula, Pertti</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution and Characterization of Neuropeptide FF-like Immunoreactivity in the Rat Nervous System with a Monoclonal Antibody</atitle><jtitle>The European journal of neuroscience</jtitle><addtitle>Eur J Neurosci</addtitle><date>1993-10</date><risdate>1993</risdate><volume>5</volume><issue>10</issue><spage>1339</spage><epage>1348</epage><pages>1339-1348</pages><issn>0953-816X</issn><eissn>1460-9568</eissn><abstract>Monoclonal antibodies against neuropeptide FF were produced and characterized. The antibodies are directed and highly specific to neuropeptide FF, and reactivity requires the C‐terminal dipeptide of neuropeptide FF (Arg‐Phe‐NH2). Tissue extracts from bovine spinal cord, rat spinal cord and hypothalamus were analysed by high‐pressure liquid chromatography coupled with radioimmunoassay using the characterized monoclonal antibody. Only one immunoreactive peptide was detected and it coeluted with authentic neuropeptide FF. Using this highly specific monoclonal antibody, the distribution of neuropeptide FF‐like immunoreactivity was further studied by indirect immunohistochemistry. Immunoreactivity was seen in two major cell groups in the rat brain. The largest cell group was located in the medial hypothalamus between the dorsomedial and ventromedial nuclei. The other one was found in the nucleus of the solitary tract. Fibres immunoreactive for neuropeptide FF were located in the lateral septal nucleus, amygdala, different hypothalamic areas, nucleus of the solitary tract, ventral medulla, trigeminal complex and the dorsal horn of the spinal cord. Spinal and sympathetic ganglia were non‐reactive. No neuropeptide FF immunoreactivity was seen in the gut autonomic nervous system or endocrine cells. The results show that neuropeptide FF‐like immunoreactivity has a clearly more limited distribution in the nervous system than typical brain–gut peptides.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8275233</pmid><doi>10.1111/j.1460-9568.1993.tb00920.x</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0953-816X |
| ispartof | The European journal of neuroscience, 1993-10, Vol.5 (10), p.1339-1348 |
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| subjects | Amino Acid Sequence analgesia Animals Antibodies, Monoclonal Antibody Specificity Biological and medical sciences Cattle Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Chromatography, High Pressure Liquid Dipeptides - analysis Enzyme-Linked Immunosorbent Assay Fundamental and applied biological sciences. Psychology hypothalamus Hypothalamus - chemistry Hypothalamus - cytology Immunohistochemistry Male Molecular Sequence Data morphine Nerve Fibers - ultrastructure Neurons - cytology neuropeptide FF Neuropeptides - analysis nucleus of the solitary tract Oligopeptides - analysis Organ Specificity Prosencephalon - cytology Radioimmunoassay Rats Rats, Sprague-Dawley Spinal Cord - chemistry Spinal Cord - cytology Vertebrates: nervous system and sense organs |
| title | Distribution and Characterization of Neuropeptide FF-like Immunoreactivity in the Rat Nervous System with a Monoclonal Antibody |
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