Identification of caprine arthritis-encephalitis retrovirus proteins in immunodiffusion precipitin lines
1 Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture and the Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164, U.S.A. Precipitin lines formed between serum from a goat...
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| Veröffentlicht in: | Journal of general virology 1985-05, Vol.66 (5), p.1139-1143 |
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| container_title | Journal of general virology |
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| creator | Adams, D.S Gogolewski, R.P Barbet, A.F Cheevers, W.P |
| description | 1 Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture and the Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164, U.S.A.
Precipitin lines formed between serum from a goat infected with caprine arthritis-encephalitis virus (CAEV) and radiolabelled viral proteins in polyethylene glycolconcentrated culture medium were excised from immunodiffusion (ID) plates and analysed by polyacrylamide gel electrophoresis. The two major precipitin lines contained the 135000 mol. wt. glycoprotein (gp135) and the internal 28000 mol. wt. structural protein (p28). This method obviates the use of purified proteins or monospecific antisera to positively determine viral constituents in ID precipitin lines formed between a crude antigen preparation and antiserum against whole virus.
Keywords: CAEV, serum, proteins
Received 12 September 1984;
accepted 14 January 1985. |
| doi_str_mv | 10.1099/0022-1317-66-5-1139 |
| format | Article |
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Precipitin lines formed between serum from a goat infected with caprine arthritis-encephalitis virus (CAEV) and radiolabelled viral proteins in polyethylene glycolconcentrated culture medium were excised from immunodiffusion (ID) plates and analysed by polyacrylamide gel electrophoresis. The two major precipitin lines contained the 135000 mol. wt. glycoprotein (gp135) and the internal 28000 mol. wt. structural protein (p28). This method obviates the use of purified proteins or monospecific antisera to positively determine viral constituents in ID precipitin lines formed between a crude antigen preparation and antiserum against whole virus.
Keywords: CAEV, serum, proteins
Received 12 September 1984;
accepted 14 January 1985.</description><identifier>ISSN: 0022-1317</identifier><identifier>EISSN: 1465-2099</identifier><identifier>DOI: 10.1099/0022-1317-66-5-1139</identifier><identifier>PMID: 3923155</identifier><identifier>CODEN: JGVIAY</identifier><language>eng</language><publisher>Reading: Soc General Microbiol</publisher><subject>Animals ; Antigens, Viral ; arthritis ; Arthritis, Infectious - microbiology ; Arthritis, Infectious - veterinary ; Biological and medical sciences ; Electrophoresis, Polyacrylamide Gel ; encephalitis ; Encephalitis - microbiology ; Encephalitis - veterinary ; Fundamental and applied biological sciences. Psychology ; Glycoproteins - analysis ; Glycoproteins - immunology ; goats ; Goats - microbiology ; Immunodiffusion ; immunodiffusion tests ; Microbiology ; Molecular Weight ; Retroviridae ; Retroviridae - analysis ; Retroviridae - immunology ; Techniques used in virology ; Viral Proteins - analysis ; Viral Proteins - immunology ; Viral Structural Proteins ; Virology</subject><ispartof>Journal of general virology, 1985-05, Vol.66 (5), p.1139-1143</ispartof><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c462t-7ad435da6a7fed4e5dfb80f9ac7d11c94373d05d04a5d46eed95ab25bb04ddc03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,3733,3734,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8421964$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3923155$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Adams, D.S</creatorcontrib><creatorcontrib>Gogolewski, R.P</creatorcontrib><creatorcontrib>Barbet, A.F</creatorcontrib><creatorcontrib>Cheevers, W.P</creatorcontrib><title>Identification of caprine arthritis-encephalitis retrovirus proteins in immunodiffusion precipitin lines</title><title>Journal of general virology</title><addtitle>J Gen Virol</addtitle><description>1 Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture and the Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164, U.S.A.
Precipitin lines formed between serum from a goat infected with caprine arthritis-encephalitis virus (CAEV) and radiolabelled viral proteins in polyethylene glycolconcentrated culture medium were excised from immunodiffusion (ID) plates and analysed by polyacrylamide gel electrophoresis. The two major precipitin lines contained the 135000 mol. wt. glycoprotein (gp135) and the internal 28000 mol. wt. structural protein (p28). This method obviates the use of purified proteins or monospecific antisera to positively determine viral constituents in ID precipitin lines formed between a crude antigen preparation and antiserum against whole virus.
Keywords: CAEV, serum, proteins
Received 12 September 1984;
accepted 14 January 1985.</description><subject>Animals</subject><subject>Antigens, Viral</subject><subject>arthritis</subject><subject>Arthritis, Infectious - microbiology</subject><subject>Arthritis, Infectious - veterinary</subject><subject>Biological and medical sciences</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>encephalitis</subject><subject>Encephalitis - microbiology</subject><subject>Encephalitis - veterinary</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycoproteins - analysis</subject><subject>Glycoproteins - immunology</subject><subject>goats</subject><subject>Goats - microbiology</subject><subject>Immunodiffusion</subject><subject>immunodiffusion tests</subject><subject>Microbiology</subject><subject>Molecular Weight</subject><subject>Retroviridae</subject><subject>Retroviridae - analysis</subject><subject>Retroviridae - immunology</subject><subject>Techniques used in virology</subject><subject>Viral Proteins - analysis</subject><subject>Viral Proteins - immunology</subject><subject>Viral Structural Proteins</subject><subject>Virology</subject><issn>0022-1317</issn><issn>1465-2099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS1EVYbCL0CILBBiY_A7k2VV8ahUqYvSteXY1xOjxA52AuLf19GMRuxYWdb5zvH1uQi9oeQTJV33mRDGMOW0xUphiSnl3TO0o0JJzKr-HO3OxAv0spSfhFAhZHuJLnnHOJVyh4ZbB3EJPlizhBSb5Btr5hwiNCYvQw5LKBiihXkw43ZpMiw5_Q55Lc2c0wIhlibEJkzTGpML3q9lC5oz2DBXR2zGmlZeoQtvxgKvT-cVevz65cfNd3x3_-325voOW6HYglvjBJfOKNN6cAKk8_2e-M7Y1lFqO8Fb7oh0RBjphAJwnTQ9k31PhHOW8Cv04Zhbh_u1Qln0FIqFcTQR0lp0qyhvBaH_Balgcs8lryA_gjanUjJ4XfuZTP6rKdHbIvRWs95q1kppqbdFVNfbU_zaT-DOnlPzVX9_0k2xZvTZRBvKGdsLRjslKvbxiA3hMPwJGfQB4hTqKH1Iuq7h3xffHVFvkjaHXNMeH1j9KWFKUtYK_gQnBqsM</recordid><startdate>198505</startdate><enddate>198505</enddate><creator>Adams, D.S</creator><creator>Gogolewski, R.P</creator><creator>Barbet, A.F</creator><creator>Cheevers, W.P</creator><general>Soc General Microbiol</general><general>Society for General Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>198505</creationdate><title>Identification of caprine arthritis-encephalitis retrovirus proteins in immunodiffusion precipitin lines</title><author>Adams, D.S ; Gogolewski, R.P ; Barbet, A.F ; Cheevers, W.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c462t-7ad435da6a7fed4e5dfb80f9ac7d11c94373d05d04a5d46eed95ab25bb04ddc03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Antigens, Viral</topic><topic>arthritis</topic><topic>Arthritis, Infectious - microbiology</topic><topic>Arthritis, Infectious - veterinary</topic><topic>Biological and medical sciences</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>encephalitis</topic><topic>Encephalitis - microbiology</topic><topic>Encephalitis - veterinary</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycoproteins - analysis</topic><topic>Glycoproteins - immunology</topic><topic>goats</topic><topic>Goats - microbiology</topic><topic>Immunodiffusion</topic><topic>immunodiffusion tests</topic><topic>Microbiology</topic><topic>Molecular Weight</topic><topic>Retroviridae</topic><topic>Retroviridae - analysis</topic><topic>Retroviridae - immunology</topic><topic>Techniques used in virology</topic><topic>Viral Proteins - analysis</topic><topic>Viral Proteins - immunology</topic><topic>Viral Structural Proteins</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adams, D.S</creatorcontrib><creatorcontrib>Gogolewski, R.P</creatorcontrib><creatorcontrib>Barbet, A.F</creatorcontrib><creatorcontrib>Cheevers, W.P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of general virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adams, D.S</au><au>Gogolewski, R.P</au><au>Barbet, A.F</au><au>Cheevers, W.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of caprine arthritis-encephalitis retrovirus proteins in immunodiffusion precipitin lines</atitle><jtitle>Journal of general virology</jtitle><addtitle>J Gen Virol</addtitle><date>1985-05</date><risdate>1985</risdate><volume>66</volume><issue>5</issue><spage>1139</spage><epage>1143</epage><pages>1139-1143</pages><issn>0022-1317</issn><eissn>1465-2099</eissn><coden>JGVIAY</coden><abstract>1 Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture and the Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164, U.S.A.
Precipitin lines formed between serum from a goat infected with caprine arthritis-encephalitis virus (CAEV) and radiolabelled viral proteins in polyethylene glycolconcentrated culture medium were excised from immunodiffusion (ID) plates and analysed by polyacrylamide gel electrophoresis. The two major precipitin lines contained the 135000 mol. wt. glycoprotein (gp135) and the internal 28000 mol. wt. structural protein (p28). This method obviates the use of purified proteins or monospecific antisera to positively determine viral constituents in ID precipitin lines formed between a crude antigen preparation and antiserum against whole virus.
Keywords: CAEV, serum, proteins
Received 12 September 1984;
accepted 14 January 1985.</abstract><cop>Reading</cop><pub>Soc General Microbiol</pub><pmid>3923155</pmid><doi>10.1099/0022-1317-66-5-1139</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Antigens, Viral arthritis Arthritis, Infectious - microbiology Arthritis, Infectious - veterinary Biological and medical sciences Electrophoresis, Polyacrylamide Gel encephalitis Encephalitis - microbiology Encephalitis - veterinary Fundamental and applied biological sciences. Psychology Glycoproteins - analysis Glycoproteins - immunology goats Goats - microbiology Immunodiffusion immunodiffusion tests Microbiology Molecular Weight Retroviridae Retroviridae - analysis Retroviridae - immunology Techniques used in virology Viral Proteins - analysis Viral Proteins - immunology Viral Structural Proteins Virology |
| title | Identification of caprine arthritis-encephalitis retrovirus proteins in immunodiffusion precipitin lines |
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