Immunization with fibronectin binding protein from Staphylococcus aureus protects against experimental endocarditis in rats
Rats were immunized with a fusion protein (gal-FnBP) encompassing β-galactosidase and the domains of fibronectin binding protein from Staphylococcus aureus responsible for binding to fibronectin. Antibodies against gal-FnBP were shown to block the binding of S. aureus to immobilized fibronectin in v...
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| Veröffentlicht in: | Microbial pathogenesis 1993-09, Vol.15 (3), p.227-236 |
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| container_title | Microbial pathogenesis |
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| creator | Schennings, Torgny Heimdahl, Anders Coster, Karin Flock, Jan-Ingmar |
| description | Rats were immunized with a fusion protein (gal-FnBP) encompassing β-galactosidase and the domains of fibronectin binding protein from
Staphylococcus aureus responsible for binding to fibronectin. Antibodies against gal-FnBP were shown to block the binding of
S. aureus to immobilized fibronectin
in vitro. Endocarditis in immunized and non-immunized control rats was induced by catheterization via the right carotid artery, resulting in damaged aortic heart valves which became covered by fibrinogen and fibronectin. The catheterized rats were then infected intravenously with 1×10
5 cells of
S. aureus. The number of bacteria associated with aortic valves was determined 1
1
2
days after the challenge infection and a significant difference in bacterial numbers between immunized and non-immunized groups was then observed (
p < 0.05). |
| doi_str_mv | 10.1006/mpat.1993.1073 |
| format | Article |
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Staphylococcus aureus responsible for binding to fibronectin. Antibodies against gal-FnBP were shown to block the binding of
S. aureus to immobilized fibronectin
in vitro. Endocarditis in immunized and non-immunized control rats was induced by catheterization via the right carotid artery, resulting in damaged aortic heart valves which became covered by fibrinogen and fibronectin. The catheterized rats were then infected intravenously with 1×10
5 cells of
S. aureus. The number of bacteria associated with aortic valves was determined 1
1
2
days after the challenge infection and a significant difference in bacterial numbers between immunized and non-immunized groups was then observed (
p < 0.05).</description><identifier>ISSN: 0882-4010</identifier><identifier>EISSN: 1096-1208</identifier><identifier>DOI: 10.1006/mpat.1993.1073</identifier><identifier>PMID: 8271922</identifier><identifier>CODEN: MIPAEV</identifier><language>eng</language><publisher>Oxford: Elsevier India Pvt Ltd</publisher><subject>Adhesins, Bacterial ; Animals ; Antibodies, Bacterial - blood ; Aortic Valve - microbiology ; Bacterial Adhesion ; Bacterial Outer Membrane Proteins - immunology ; Bacterial Outer Membrane Proteins - metabolism ; Bacterial Proteins ; Bacteriology ; Binding, Competitive ; Biological and medical sciences ; Carrier Proteins ; Endocarditis, Bacterial - microbiology ; Endocarditis, Bacterial - prevention & control ; Enzyme-Linked Immunosorbent Assay ; Female ; Fibronectins - metabolism ; Fundamental and applied biological sciences. Psychology ; Immunoglobulin G - blood ; Microbiology ; Rats ; Rats, Wistar ; Recombinant Fusion Proteins - genetics ; Recombinant Fusion Proteins - immunology ; Staphylococcal Infections - prevention & control ; Staphylococcal Vaccines - immunology ; Staphylococcus aureus - immunology ; Staphylococcus aureus - metabolism ; Staphylococcus aureus; adhesion; endocarditis; immunization; fibronectin binding protein ; Vaccination ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Vaccines, Synthetic - immunology</subject><ispartof>Microbial pathogenesis, 1993-09, Vol.15 (3), p.227-236</ispartof><rights>1993 Academic Press</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-f7782829d61fe45611ea2a27eb9e441fa8b9ac5ac43a731fabcb0b7993ecb43</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/mpat.1993.1073$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3796919$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8271922$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Schennings, Torgny</creatorcontrib><creatorcontrib>Heimdahl, Anders</creatorcontrib><creatorcontrib>Coster, Karin</creatorcontrib><creatorcontrib>Flock, Jan-Ingmar</creatorcontrib><title>Immunization with fibronectin binding protein from Staphylococcus aureus protects against experimental endocarditis in rats</title><title>Microbial pathogenesis</title><addtitle>Microb Pathog</addtitle><description>Rats were immunized with a fusion protein (gal-FnBP) encompassing β-galactosidase and the domains of fibronectin binding protein from
Staphylococcus aureus responsible for binding to fibronectin. Antibodies against gal-FnBP were shown to block the binding of
S. aureus to immobilized fibronectin
in vitro. Endocarditis in immunized and non-immunized control rats was induced by catheterization via the right carotid artery, resulting in damaged aortic heart valves which became covered by fibrinogen and fibronectin. The catheterized rats were then infected intravenously with 1×10
5 cells of
S. aureus. The number of bacteria associated with aortic valves was determined 1
1
2
days after the challenge infection and a significant difference in bacterial numbers between immunized and non-immunized groups was then observed (
p < 0.05).</description><subject>Adhesins, Bacterial</subject><subject>Animals</subject><subject>Antibodies, Bacterial - blood</subject><subject>Aortic Valve - microbiology</subject><subject>Bacterial Adhesion</subject><subject>Bacterial Outer Membrane Proteins - immunology</subject><subject>Bacterial Outer Membrane Proteins - metabolism</subject><subject>Bacterial Proteins</subject><subject>Bacteriology</subject><subject>Binding, Competitive</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins</subject><subject>Endocarditis, Bacterial - microbiology</subject><subject>Endocarditis, Bacterial - prevention & control</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Fibronectins - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunoglobulin G - blood</subject><subject>Microbiology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Recombinant Fusion Proteins - genetics</subject><subject>Recombinant Fusion Proteins - immunology</subject><subject>Staphylococcal Infections - prevention & control</subject><subject>Staphylococcal Vaccines - immunology</subject><subject>Staphylococcus aureus - immunology</subject><subject>Staphylococcus aureus - metabolism</subject><subject>Staphylococcus aureus; adhesion; endocarditis; immunization; fibronectin binding protein</subject><subject>Vaccination</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Vaccines, Synthetic - immunology</subject><issn>0882-4010</issn><issn>1096-1208</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1v1DAQhi0EKkvhyg0pB8Qtiz-ysX1EVaGVKnEod2vsTFqjxA62Ay38eRx21Run0at5PH71EPKW0T2jtP84L1D2TGtRoxTPyI5R3beMU_Wc7KhSvO0ooy_Jq5y_U0p1J_QZOVNcMs35jvy5nuc1-N9QfAzNL1_um9HbFAO64kNjfRh8uGuWFAvWPKY4N7cFlvvHKbro3JobWBPW8Q9xpeY78CGXBh8WTH7GUGBqMAzRQRp88bmphxKU_Jq8GGHK-OY0z8nt58tvF1ftzdcv1xefblonelXaUUrFFddDz0bsDj1jCBy4RKux69gIympwB3CdAClqts5SK6sSdLYT5-TD8Wot-GPFXMzss8NpgoBxzUb2TBwOXFdwfwRdijknHM1S60N6NIyazbXZXJvNtdlc1wfvTpdXO-PwhJ_k1v370x6yg2lMEJzPT5iQutds-1cdMawOfnpMJjuPweHgUxVqhuj_1-AvJjmfLw</recordid><startdate>19930901</startdate><enddate>19930901</enddate><creator>Schennings, Torgny</creator><creator>Heimdahl, Anders</creator><creator>Coster, Karin</creator><creator>Flock, Jan-Ingmar</creator><general>Elsevier India Pvt Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930901</creationdate><title>Immunization with fibronectin binding protein from Staphylococcus aureus protects against experimental endocarditis in rats</title><author>Schennings, Torgny ; Heimdahl, Anders ; Coster, Karin ; Flock, Jan-Ingmar</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-f7782829d61fe45611ea2a27eb9e441fa8b9ac5ac43a731fabcb0b7993ecb43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adhesins, Bacterial</topic><topic>Animals</topic><topic>Antibodies, Bacterial - blood</topic><topic>Aortic Valve - microbiology</topic><topic>Bacterial Adhesion</topic><topic>Bacterial Outer Membrane Proteins - immunology</topic><topic>Bacterial Outer Membrane Proteins - metabolism</topic><topic>Bacterial Proteins</topic><topic>Bacteriology</topic><topic>Binding, Competitive</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins</topic><topic>Endocarditis, Bacterial - microbiology</topic><topic>Endocarditis, Bacterial - prevention & control</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Fibronectins - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunoglobulin G - blood</topic><topic>Microbiology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Recombinant Fusion Proteins - genetics</topic><topic>Recombinant Fusion Proteins - immunology</topic><topic>Staphylococcal Infections - prevention & control</topic><topic>Staphylococcal Vaccines - immunology</topic><topic>Staphylococcus aureus - immunology</topic><topic>Staphylococcus aureus - metabolism</topic><topic>Staphylococcus aureus; adhesion; endocarditis; immunization; fibronectin binding protein</topic><topic>Vaccination</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Vaccines, Synthetic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schennings, Torgny</creatorcontrib><creatorcontrib>Heimdahl, Anders</creatorcontrib><creatorcontrib>Coster, Karin</creatorcontrib><creatorcontrib>Flock, Jan-Ingmar</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Microbial pathogenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schennings, Torgny</au><au>Heimdahl, Anders</au><au>Coster, Karin</au><au>Flock, Jan-Ingmar</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunization with fibronectin binding protein from Staphylococcus aureus protects against experimental endocarditis in rats</atitle><jtitle>Microbial pathogenesis</jtitle><addtitle>Microb Pathog</addtitle><date>1993-09-01</date><risdate>1993</risdate><volume>15</volume><issue>3</issue><spage>227</spage><epage>236</epage><pages>227-236</pages><issn>0882-4010</issn><eissn>1096-1208</eissn><coden>MIPAEV</coden><abstract>Rats were immunized with a fusion protein (gal-FnBP) encompassing β-galactosidase and the domains of fibronectin binding protein from
Staphylococcus aureus responsible for binding to fibronectin. Antibodies against gal-FnBP were shown to block the binding of
S. aureus to immobilized fibronectin
in vitro. Endocarditis in immunized and non-immunized control rats was induced by catheterization via the right carotid artery, resulting in damaged aortic heart valves which became covered by fibrinogen and fibronectin. The catheterized rats were then infected intravenously with 1×10
5 cells of
S. aureus. The number of bacteria associated with aortic valves was determined 1
1
2
days after the challenge infection and a significant difference in bacterial numbers between immunized and non-immunized groups was then observed (
p < 0.05).</abstract><cop>Oxford</cop><pub>Elsevier India Pvt Ltd</pub><pmid>8271922</pmid><doi>10.1006/mpat.1993.1073</doi><tpages>10</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Adhesins, Bacterial Animals Antibodies, Bacterial - blood Aortic Valve - microbiology Bacterial Adhesion Bacterial Outer Membrane Proteins - immunology Bacterial Outer Membrane Proteins - metabolism Bacterial Proteins Bacteriology Binding, Competitive Biological and medical sciences Carrier Proteins Endocarditis, Bacterial - microbiology Endocarditis, Bacterial - prevention & control Enzyme-Linked Immunosorbent Assay Female Fibronectins - metabolism Fundamental and applied biological sciences. Psychology Immunoglobulin G - blood Microbiology Rats Rats, Wistar Recombinant Fusion Proteins - genetics Recombinant Fusion Proteins - immunology Staphylococcal Infections - prevention & control Staphylococcal Vaccines - immunology Staphylococcus aureus - immunology Staphylococcus aureus - metabolism Staphylococcus aureus adhesion endocarditis immunization fibronectin binding protein Vaccination Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Vaccines, Synthetic - immunology |
| title | Immunization with fibronectin binding protein from Staphylococcus aureus protects against experimental endocarditis in rats |
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