Expression of the human foamy virus bel-1 transactivator in insect cells
The human foamy virus (HFV) bel-1 transactivator protein was expressed in insect cells by a recombinant baculovirus. For the generation of the recombinant baculovirus, Acbel-1, the bel-1 gene of an HFV mutant was used, that bears truncations in the bel-1 overlapping bel-2 open reading frame. Acbel-1...
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| Veröffentlicht in: | Virus research 1993-10, Vol.30 (1), p.89-95 |
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| creator | Hong, Liu Bräutigam, Sandra Rethwilm, Axel |
| description | The human foamy virus (HFV) bel-1 transactivator protein was expressed in insect cells by a recombinant baculovirus. For the generation of the recombinant baculovirus, Acbel-1, the bel-1 gene of an HFV mutant was used, that bears truncations in the bel-1 overlapping bel-2 open reading frame. Acbel-1 infected Sf9 cells produced high amounts of recombinant protein of the same electrophoretic mobility (36 kD) as bel-1 expressed in mammalian cells. The baculovirus expressed bel-1 protein was readily identified by a polyclonal rabbit serum directed against bel-1 in immunoblot assay. As in mammalian cells, bel-1 was predominantly localized to the nucleus of Acbel-1 infected insect cells. The baculovirus expressed bel-1 protein will be of use to determine the action of this novel viral transactivator more precisely. |
| doi_str_mv | 10.1016/0168-1702(93)90018-I |
| format | Article |
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For the generation of the recombinant baculovirus, Acbel-1, the bel-1 gene of an HFV mutant was used, that bears truncations in the bel-1 overlapping bel-2 open reading frame. Acbel-1 infected Sf9 cells produced high amounts of recombinant protein of the same electrophoretic mobility (36 kD) as bel-1 expressed in mammalian cells. The baculovirus expressed bel-1 protein was readily identified by a polyclonal rabbit serum directed against bel-1 in immunoblot assay. As in mammalian cells, bel-1 was predominantly localized to the nucleus of Acbel-1 infected insect cells. The baculovirus expressed bel-1 protein will be of use to determine the action of this novel viral transactivator more precisely.</description><identifier>ISSN: 0168-1702</identifier><identifier>EISSN: 1872-7492</identifier><identifier>DOI: 10.1016/0168-1702(93)90018-I</identifier><identifier>PMID: 8266722</identifier><identifier>CODEN: VIREDF</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; baculovirus ; Biological and medical sciences ; DNA-Binding Proteins - biosynthesis ; DNA-Binding Proteins - genetics ; Expression in insect cells ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Viral ; Human foamy virus bel-1 transactivator ; Insecta - cytology ; Insecta - genetics ; Microbiology ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Retroviridae Proteins - biosynthesis ; Retroviridae Proteins - genetics ; Spumavirus - genetics ; Trans-Activators - biosynthesis ; Trans-Activators - genetics ; Virology</subject><ispartof>Virus research, 1993-10, Vol.30 (1), p.89-95</ispartof><rights>1993</rights><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c412t-60a4c5b559ff153f9a2fc65a7118564bdc9713f7eb0eae94a60200386bfc0db13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0168-1702(93)90018-I$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3795614$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8266722$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hong, Liu</creatorcontrib><creatorcontrib>Bräutigam, Sandra</creatorcontrib><creatorcontrib>Rethwilm, Axel</creatorcontrib><title>Expression of the human foamy virus bel-1 transactivator in insect cells</title><title>Virus research</title><addtitle>Virus Res</addtitle><description>The human foamy virus (HFV) bel-1 transactivator protein was expressed in insect cells by a recombinant baculovirus. For the generation of the recombinant baculovirus, Acbel-1, the bel-1 gene of an HFV mutant was used, that bears truncations in the bel-1 overlapping bel-2 open reading frame. Acbel-1 infected Sf9 cells produced high amounts of recombinant protein of the same electrophoretic mobility (36 kD) as bel-1 expressed in mammalian cells. The baculovirus expressed bel-1 protein was readily identified by a polyclonal rabbit serum directed against bel-1 in immunoblot assay. As in mammalian cells, bel-1 was predominantly localized to the nucleus of Acbel-1 infected insect cells. The baculovirus expressed bel-1 protein will be of use to determine the action of this novel viral transactivator more precisely.</description><subject>Animals</subject><subject>baculovirus</subject><subject>Biological and medical sciences</subject><subject>DNA-Binding Proteins - biosynthesis</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Expression in insect cells</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Viral</subject><subject>Human foamy virus bel-1 transactivator</subject><subject>Insecta - cytology</subject><subject>Insecta - genetics</subject><subject>Microbiology</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Retroviridae Proteins - biosynthesis</subject><subject>Retroviridae Proteins - genetics</subject><subject>Spumavirus - genetics</subject><subject>Trans-Activators - biosynthesis</subject><subject>Trans-Activators - genetics</subject><subject>Virology</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtr3DAQgEVp2W7S_oMWdCglOTjVw5asS6AseSwEcmnPQpZHrIofG429JP--dtbsMYEZ5jDfPPgI-cbZFWdc_ZqyzLhm4sLIS8MYL7PtB7LmpRaZzo34SNYn5DM5Q_zHGFNSqxVZlUIpLcSa3N887xMgxr6jfaDDDuhubF1HQ-_aF3qIaURaQZNxOiTXofNDPLihTzR2UyD4gXpoGvxCPgXXIHxd6jn5e3vzZ3OfPTzebTe_HzKfczFkirncF1VRmBB4IYNxInhVOM15Wai8qr3RXAYNFQMHJneKCcZkqargWV1xeU5-HvfuU_80Ag62jTh_4DroR7RacVlIkb8LTmqMUWoG8yPoU4-YINh9iq1LL5YzO5u2s0Y7a7RG2lfTdjuNfV_2j1UL9WloUTv1fyx9h941YbLnI54wqU2h-Hz9-ojBJO0QIVn0EToPdUyTXFv38e0__gMtTJk0</recordid><startdate>19931001</startdate><enddate>19931001</enddate><creator>Hong, Liu</creator><creator>Bräutigam, Sandra</creator><creator>Rethwilm, Axel</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19931001</creationdate><title>Expression of the human foamy virus bel-1 transactivator in insect cells</title><author>Hong, Liu ; Bräutigam, Sandra ; Rethwilm, Axel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c412t-60a4c5b559ff153f9a2fc65a7118564bdc9713f7eb0eae94a60200386bfc0db13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>baculovirus</topic><topic>Biological and medical sciences</topic><topic>DNA-Binding Proteins - biosynthesis</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Expression in insect cells</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Viral</topic><topic>Human foamy virus bel-1 transactivator</topic><topic>Insecta - cytology</topic><topic>Insecta - genetics</topic><topic>Microbiology</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Retroviridae Proteins - biosynthesis</topic><topic>Retroviridae Proteins - genetics</topic><topic>Spumavirus - genetics</topic><topic>Trans-Activators - biosynthesis</topic><topic>Trans-Activators - genetics</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hong, Liu</creatorcontrib><creatorcontrib>Bräutigam, Sandra</creatorcontrib><creatorcontrib>Rethwilm, Axel</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hong, Liu</au><au>Bräutigam, Sandra</au><au>Rethwilm, Axel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of the human foamy virus bel-1 transactivator in insect cells</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>1993-10-01</date><risdate>1993</risdate><volume>30</volume><issue>1</issue><spage>89</spage><epage>95</epage><pages>89-95</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><coden>VIREDF</coden><abstract>The human foamy virus (HFV) bel-1 transactivator protein was expressed in insect cells by a recombinant baculovirus. For the generation of the recombinant baculovirus, Acbel-1, the bel-1 gene of an HFV mutant was used, that bears truncations in the bel-1 overlapping bel-2 open reading frame. Acbel-1 infected Sf9 cells produced high amounts of recombinant protein of the same electrophoretic mobility (36 kD) as bel-1 expressed in mammalian cells. The baculovirus expressed bel-1 protein was readily identified by a polyclonal rabbit serum directed against bel-1 in immunoblot assay. As in mammalian cells, bel-1 was predominantly localized to the nucleus of Acbel-1 infected insect cells. The baculovirus expressed bel-1 protein will be of use to determine the action of this novel viral transactivator more precisely.</abstract><cop>London</cop><cop>Shannon</cop><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8266722</pmid><doi>10.1016/0168-1702(93)90018-I</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Virus research, 1993-10, Vol.30 (1), p.89-95 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals baculovirus Biological and medical sciences DNA-Binding Proteins - biosynthesis DNA-Binding Proteins - genetics Expression in insect cells Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Viral Human foamy virus bel-1 transactivator Insecta - cytology Insecta - genetics Microbiology Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Retroviridae Proteins - biosynthesis Retroviridae Proteins - genetics Spumavirus - genetics Trans-Activators - biosynthesis Trans-Activators - genetics Virology |
| title | Expression of the human foamy virus bel-1 transactivator in insect cells |
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