Heterogeneity of Basal Keratinocytes: Nonrandom Distribution of Thymidine-Labeled Basal Cells in Confluent Cultures Is Not a Technical Artifact

Basal surface autoradiography of [3H]dThd-labeled, confluent, keratinocyte cultures reveals that proliferating cells have a nonrandom, patterned distribution. Unlabeled cells, likewise, appear nonrandomly in clusters. We show here that failure to detect DNA synthesis in some basal cells in culture i...

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Veröffentlicht in:	J. Invest. Dermatol.; (United States) 1985-06, Vol.84 (6), p.504-507
Hauptverfasser:	Milstone, Leonard M., LaVigne, Jane F.
Format:	Artikel
Sprache:	eng
Schlagworte:	550201 - Biochemistry- Tracer Techniques ANIMAL CELLS ANIMAL TISSUES AUTORADIOGRAPHY AZINES BASIC BIOLOGICAL SCIENCES Biological and medical sciences BODY CELL CONSTITUENTS CELL CULTURES CELL NUCLEI CELL PROLIFERATION Cell Separation Cells, Cultured DIFFUSION DNA EPIDERMIS Epithelial Cells EPITHELIUM Ethidium - pharmacology Fundamental and applied biological sciences. Psychology HETEROCYCLIC COMPOUNDS Humans ISOTOPE APPLICATIONS KERATIN LABELLED COMPOUNDS Male Microscopy, Fluorescence NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS ORGANS PROTEINS PYRIMIDINES RIBOSIDES SCLEROPROTEINS SKIN Skin - cytology THYMIDINE Time Factors TISSUES TRACER TECHNIQUES Tritium TRITIUM COMPOUNDS Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue
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