Heterogeneity of Basal Keratinocytes: Nonrandom Distribution of Thymidine-Labeled Basal Cells in Confluent Cultures Is Not a Technical Artifact
Basal surface autoradiography of [3H]dThd-labeled, confluent, keratinocyte cultures reveals that proliferating cells have a nonrandom, patterned distribution. Unlabeled cells, likewise, appear nonrandomly in clusters. We show here that failure to detect DNA synthesis in some basal cells in culture i...
Gespeichert in:
| Veröffentlicht in: | J. Invest. Dermatol.; (United States) 1985-06, Vol.84 (6), p.504-507 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 507 |
|---|---|
| container_issue | 6 |
| container_start_page | 504 |
| container_title | J. Invest. Dermatol.; (United States) |
| container_volume | 84 |
| creator | Milstone, Leonard M. LaVigne, Jane F. |
| description | Basal surface autoradiography of [3H]dThd-labeled, confluent, keratinocyte cultures reveals that proliferating cells have a nonrandom, patterned distribution. Unlabeled cells, likewise, appear nonrandomly in clusters. We show here that failure to detect DNA synthesis in some basal cells in culture is not an artifact caused either by physical separation of the labeled nuclei from the radiographic emulsion or by a diffusion barrier that would prevent [3H]dThd from reaching basal cells. |
| doi_str_mv | 10.1111/1523-1747.ep12273479 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_osti_</sourceid><recordid>TN_cdi_proquest_miscellaneous_76131929</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0022202X15436772</els_id><sourcerecordid>76131929</sourcerecordid><originalsourceid>FETCH-LOGICAL-c459t-21dcbf65298389361cc647e5a618e3544f49d84902aaf185c929bb817717e10b3</originalsourceid><addsrcrecordid>eNp9kc-KFDEQxoMo67j6BgpBxFuvSTrdSTwIa_tnFwe9jOAtpNPVbqQnmU3SwjyFr2yaacabuRSkfvVRX30IPafkipb3hjasrqjg4goOlDFRc6EeoM35-yHaEMJYxQj78Rg9SekXIbTljbxAF7VSkiu5QX9uIEMMP8GDy0ccRvzeJDPhLxBNdj7YY4b0Fn8NPho_hD3-4FKOrp-zC37Bd3fHvRuch2prephgWAU6mKaEncdd8OM0g8-4m6c8R0j4NhXBjA3egb3zzhb8OmY3GpufokejmRI8W-sl-v7p4667qbbfPt9219vK8kblitHB9mPbMCVrqeqWWttyAY1pqYS64XzkaigOCTNmpLKxiqm-l1QIKoCSvr5EL0-6IWWnk3W5rGKD92CzbmtJhOAFen2CDjHcz5Cy3rtkizHjIcxJi5bWtCgXkJ9AG0NKEUZ9iG5v4lFTopew9JKKXlLR_8IqYy9W_bnfw3AeWtMp_Vdr36RypLFEYF06Y5IzRptF5t0Jg3Kw3w7i4ge8hcHFxc4Q3P_3-AuANrEs</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76131929</pqid></control><display><type>article</type><title>Heterogeneity of Basal Keratinocytes: Nonrandom Distribution of Thymidine-Labeled Basal Cells in Confluent Cultures Is Not a Technical Artifact</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Milstone, Leonard M. ; LaVigne, Jane F.</creator><creatorcontrib>Milstone, Leonard M. ; LaVigne, Jane F.</creatorcontrib><description>Basal surface autoradiography of [3H]dThd-labeled, confluent, keratinocyte cultures reveals that proliferating cells have a nonrandom, patterned distribution. Unlabeled cells, likewise, appear nonrandomly in clusters. We show here that failure to detect DNA synthesis in some basal cells in culture is not an artifact caused either by physical separation of the labeled nuclei from the radiographic emulsion or by a diffusion barrier that would prevent [3H]dThd from reaching basal cells.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1111/1523-1747.ep12273479</identifier><identifier>PMID: 3998498</identifier><identifier>CODEN: JIDEAE</identifier><language>eng</language><publisher>Danvers, MA: Elsevier Inc</publisher><subject>550201 - Biochemistry- Tracer Techniques ; ANIMAL CELLS ; ANIMAL TISSUES ; AUTORADIOGRAPHY ; AZINES ; BASIC BIOLOGICAL SCIENCES ; Biological and medical sciences ; BODY ; CELL CONSTITUENTS ; CELL CULTURES ; CELL NUCLEI ; CELL PROLIFERATION ; Cell Separation ; Cells, Cultured ; DIFFUSION ; DNA ; EPIDERMIS ; Epithelial Cells ; EPITHELIUM ; Ethidium - pharmacology ; Fundamental and applied biological sciences. Psychology ; HETEROCYCLIC COMPOUNDS ; Humans ; ISOTOPE APPLICATIONS ; KERATIN ; LABELLED COMPOUNDS ; Male ; Microscopy, Fluorescence ; NUCLEIC ACIDS ; NUCLEOSIDES ; NUCLEOTIDES ; ORGANIC COMPOUNDS ; ORGANIC NITROGEN COMPOUNDS ; ORGANS ; PROTEINS ; PYRIMIDINES ; RIBOSIDES ; SCLEROPROTEINS ; SKIN ; Skin - cytology ; THYMIDINE ; Time Factors ; TISSUES ; TRACER TECHNIQUES ; Tritium ; TRITIUM COMPOUNDS ; Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue</subject><ispartof>J. Invest. Dermatol.; (United States), 1985-06, Vol.84 (6), p.504-507</ispartof><rights>1985 The Society for Investigative Dermatology, Inc</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-21dcbf65298389361cc647e5a618e3544f49d84902aaf185c929bb817717e10b3</citedby><cites>FETCH-LOGICAL-c459t-21dcbf65298389361cc647e5a618e3544f49d84902aaf185c929bb817717e10b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,881,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8422159$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3998498$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6380774$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Milstone, Leonard M.</creatorcontrib><creatorcontrib>LaVigne, Jane F.</creatorcontrib><title>Heterogeneity of Basal Keratinocytes: Nonrandom Distribution of Thymidine-Labeled Basal Cells in Confluent Cultures Is Not a Technical Artifact</title><title>J. Invest. Dermatol.; (United States)</title><addtitle>J Invest Dermatol</addtitle><description>Basal surface autoradiography of [3H]dThd-labeled, confluent, keratinocyte cultures reveals that proliferating cells have a nonrandom, patterned distribution. Unlabeled cells, likewise, appear nonrandomly in clusters. We show here that failure to detect DNA synthesis in some basal cells in culture is not an artifact caused either by physical separation of the labeled nuclei from the radiographic emulsion or by a diffusion barrier that would prevent [3H]dThd from reaching basal cells.</description><subject>550201 - Biochemistry- Tracer Techniques</subject><subject>ANIMAL CELLS</subject><subject>ANIMAL TISSUES</subject><subject>AUTORADIOGRAPHY</subject><subject>AZINES</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>Biological and medical sciences</subject><subject>BODY</subject><subject>CELL CONSTITUENTS</subject><subject>CELL CULTURES</subject><subject>CELL NUCLEI</subject><subject>CELL PROLIFERATION</subject><subject>Cell Separation</subject><subject>Cells, Cultured</subject><subject>DIFFUSION</subject><subject>DNA</subject><subject>EPIDERMIS</subject><subject>Epithelial Cells</subject><subject>EPITHELIUM</subject><subject>Ethidium - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HETEROCYCLIC COMPOUNDS</subject><subject>Humans</subject><subject>ISOTOPE APPLICATIONS</subject><subject>KERATIN</subject><subject>LABELLED COMPOUNDS</subject><subject>Male</subject><subject>Microscopy, Fluorescence</subject><subject>NUCLEIC ACIDS</subject><subject>NUCLEOSIDES</subject><subject>NUCLEOTIDES</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANIC NITROGEN COMPOUNDS</subject><subject>ORGANS</subject><subject>PROTEINS</subject><subject>PYRIMIDINES</subject><subject>RIBOSIDES</subject><subject>SCLEROPROTEINS</subject><subject>SKIN</subject><subject>Skin - cytology</subject><subject>THYMIDINE</subject><subject>Time Factors</subject><subject>TISSUES</subject><subject>TRACER TECHNIQUES</subject><subject>Tritium</subject><subject>TRITIUM COMPOUNDS</subject><subject>Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc-KFDEQxoMo67j6BgpBxFuvSTrdSTwIa_tnFwe9jOAtpNPVbqQnmU3SwjyFr2yaacabuRSkfvVRX30IPafkipb3hjasrqjg4goOlDFRc6EeoM35-yHaEMJYxQj78Rg9SekXIbTljbxAF7VSkiu5QX9uIEMMP8GDy0ccRvzeJDPhLxBNdj7YY4b0Fn8NPho_hD3-4FKOrp-zC37Bd3fHvRuch2prephgWAU6mKaEncdd8OM0g8-4m6c8R0j4NhXBjA3egb3zzhb8OmY3GpufokejmRI8W-sl-v7p4667qbbfPt9219vK8kblitHB9mPbMCVrqeqWWttyAY1pqYS64XzkaigOCTNmpLKxiqm-l1QIKoCSvr5EL0-6IWWnk3W5rGKD92CzbmtJhOAFen2CDjHcz5Cy3rtkizHjIcxJi5bWtCgXkJ9AG0NKEUZ9iG5v4lFTopew9JKKXlLR_8IqYy9W_bnfw3AeWtMp_Vdr36RypLFEYF06Y5IzRptF5t0Jg3Kw3w7i4ge8hcHFxc4Q3P_3-AuANrEs</recordid><startdate>198506</startdate><enddate>198506</enddate><creator>Milstone, Leonard M.</creator><creator>LaVigne, Jane F.</creator><general>Elsevier Inc</general><general>Nature Publishing</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>198506</creationdate><title>Heterogeneity of Basal Keratinocytes: Nonrandom Distribution of Thymidine-Labeled Basal Cells in Confluent Cultures Is Not a Technical Artifact</title><author>Milstone, Leonard M. ; LaVigne, Jane F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-21dcbf65298389361cc647e5a618e3544f49d84902aaf185c929bb817717e10b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>550201 - Biochemistry- Tracer Techniques</topic><topic>ANIMAL CELLS</topic><topic>ANIMAL TISSUES</topic><topic>AUTORADIOGRAPHY</topic><topic>AZINES</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>Biological and medical sciences</topic><topic>BODY</topic><topic>CELL CONSTITUENTS</topic><topic>CELL CULTURES</topic><topic>CELL NUCLEI</topic><topic>CELL PROLIFERATION</topic><topic>Cell Separation</topic><topic>Cells, Cultured</topic><topic>DIFFUSION</topic><topic>DNA</topic><topic>EPIDERMIS</topic><topic>Epithelial Cells</topic><topic>EPITHELIUM</topic><topic>Ethidium - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HETEROCYCLIC COMPOUNDS</topic><topic>Humans</topic><topic>ISOTOPE APPLICATIONS</topic><topic>KERATIN</topic><topic>LABELLED COMPOUNDS</topic><topic>Male</topic><topic>Microscopy, Fluorescence</topic><topic>NUCLEIC ACIDS</topic><topic>NUCLEOSIDES</topic><topic>NUCLEOTIDES</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANIC NITROGEN COMPOUNDS</topic><topic>ORGANS</topic><topic>PROTEINS</topic><topic>PYRIMIDINES</topic><topic>RIBOSIDES</topic><topic>SCLEROPROTEINS</topic><topic>SKIN</topic><topic>Skin - cytology</topic><topic>THYMIDINE</topic><topic>Time Factors</topic><topic>TISSUES</topic><topic>TRACER TECHNIQUES</topic><topic>Tritium</topic><topic>TRITIUM COMPOUNDS</topic><topic>Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Milstone, Leonard M.</creatorcontrib><creatorcontrib>LaVigne, Jane F.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>J. Invest. Dermatol.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Milstone, Leonard M.</au><au>LaVigne, Jane F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Heterogeneity of Basal Keratinocytes: Nonrandom Distribution of Thymidine-Labeled Basal Cells in Confluent Cultures Is Not a Technical Artifact</atitle><jtitle>J. Invest. Dermatol.; (United States)</jtitle><addtitle>J Invest Dermatol</addtitle><date>1985-06</date><risdate>1985</risdate><volume>84</volume><issue>6</issue><spage>504</spage><epage>507</epage><pages>504-507</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><coden>JIDEAE</coden><abstract>Basal surface autoradiography of [3H]dThd-labeled, confluent, keratinocyte cultures reveals that proliferating cells have a nonrandom, patterned distribution. Unlabeled cells, likewise, appear nonrandomly in clusters. We show here that failure to detect DNA synthesis in some basal cells in culture is not an artifact caused either by physical separation of the labeled nuclei from the radiographic emulsion or by a diffusion barrier that would prevent [3H]dThd from reaching basal cells.</abstract><cop>Danvers, MA</cop><pub>Elsevier Inc</pub><pmid>3998498</pmid><doi>10.1111/1523-1747.ep12273479</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-202X |
| ispartof | J. Invest. Dermatol.; (United States), 1985-06, Vol.84 (6), p.504-507 |
| issn | 0022-202X 1523-1747 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76131929 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | 550201 - Biochemistry- Tracer Techniques ANIMAL CELLS ANIMAL TISSUES AUTORADIOGRAPHY AZINES BASIC BIOLOGICAL SCIENCES Biological and medical sciences BODY CELL CONSTITUENTS CELL CULTURES CELL NUCLEI CELL PROLIFERATION Cell Separation Cells, Cultured DIFFUSION DNA EPIDERMIS Epithelial Cells EPITHELIUM Ethidium - pharmacology Fundamental and applied biological sciences. Psychology HETEROCYCLIC COMPOUNDS Humans ISOTOPE APPLICATIONS KERATIN LABELLED COMPOUNDS Male Microscopy, Fluorescence NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS ORGANS PROTEINS PYRIMIDINES RIBOSIDES SCLEROPROTEINS SKIN Skin - cytology THYMIDINE Time Factors TISSUES TRACER TECHNIQUES Tritium TRITIUM COMPOUNDS Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue |
| title | Heterogeneity of Basal Keratinocytes: Nonrandom Distribution of Thymidine-Labeled Basal Cells in Confluent Cultures Is Not a Technical Artifact |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T04%3A18%3A57IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_osti_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Heterogeneity%20of%20Basal%20Keratinocytes:%20Nonrandom%20Distribution%20of%20Thymidine-Labeled%20Basal%20Cells%20in%20Confluent%20Cultures%20Is%20Not%20a%20Technical%20Artifact&rft.jtitle=J.%20Invest.%20Dermatol.;%20(United%20States)&rft.au=Milstone,%20Leonard%20M.&rft.date=1985-06&rft.volume=84&rft.issue=6&rft.spage=504&rft.epage=507&rft.pages=504-507&rft.issn=0022-202X&rft.eissn=1523-1747&rft.coden=JIDEAE&rft_id=info:doi/10.1111/1523-1747.ep12273479&rft_dat=%3Cproquest_osti_%3E76131929%3C/proquest_osti_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76131929&rft_id=info:pmid/3998498&rft_els_id=S0022202X15436772&rfr_iscdi=true |