Identification of an allelic variant of isoform MLC1-V/sB (human myosin light chain)

A study of 250 specimens of human myocardium by two-dimensional gel electrophoresis revealed an allelic variant of isoform MLC1-V/sB, which was identified by immunoblotting with monoclonal antibody against MLC1-V/sB and peptide mapping after in situ tryptic digestion of electroblotted proteins. The...

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Veröffentlicht in:Biochemical genetics 1993-06, Vol.31 (5-6), p.253-258
Hauptverfasser: Laptev, A V, Shishkin, S S, Kovalyov, L I, Galyuk, M A, Musolyamov, A Kh, Egorov, T A
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container_end_page 258
container_issue 5-6
container_start_page 253
container_title Biochemical genetics
container_volume 31
creator Laptev, A V
Shishkin, S S
Kovalyov, L I
Galyuk, M A
Musolyamov, A Kh
Egorov, T A
description A study of 250 specimens of human myocardium by two-dimensional gel electrophoresis revealed an allelic variant of isoform MLC1-V/sB, which was identified by immunoblotting with monoclonal antibody against MLC1-V/sB and peptide mapping after in situ tryptic digestion of electroblotted proteins. The substitution Asn-144 for His-144 was found in this new allelic variant of MLC1-V/sB.
doi_str_mv 10.1007/BF00557334
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ispartof Biochemical genetics, 1993-06, Vol.31 (5-6), p.253-258
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1573-4927
language eng
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source MEDLINE; SpringerNature Journals
subjects Alleles
Amino Acid Sequence
Chromatography, High Pressure Liquid
Electrophoresis, Gel, Two-Dimensional
Genetic Variation
Humans
isoforms
isolation
light chain
man
Molecular Sequence Data
myocardium
Myocardium - chemistry
myosin
Myosins - chemistry
Myosins - genetics
variants
title Identification of an allelic variant of isoform MLC1-V/sB (human myosin light chain)
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