Identification of an allelic variant of isoform MLC1-V/sB (human myosin light chain)

A study of 250 specimens of human myocardium by two-dimensional gel electrophoresis revealed an allelic variant of isoform MLC1-V/sB, which was identified by immunoblotting with monoclonal antibody against MLC1-V/sB and peptide mapping after in situ tryptic digestion of electroblotted proteins. The...

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Veröffentlicht in:	Biochemical genetics 1993-06, Vol.31 (5-6), p.253-258
Hauptverfasser:	Laptev, A V, Shishkin, S S, Kovalyov, L I, Galyuk, M A, Musolyamov, A Kh, Egorov, T A
Format:	Artikel
Sprache:	eng
Schlagworte:	Alleles Amino Acid Sequence Chromatography, High Pressure Liquid Electrophoresis, Gel, Two-Dimensional Genetic Variation Humans isoforms isolation light chain man Molecular Sequence Data myocardium Myocardium - chemistry myosin Myosins - chemistry Myosins - genetics variants
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Zusammenfassung:	A study of 250 specimens of human myocardium by two-dimensional gel electrophoresis revealed an allelic variant of isoform MLC1-V/sB, which was identified by immunoblotting with monoclonal antibody against MLC1-V/sB and peptide mapping after in situ tryptic digestion of electroblotted proteins. The substitution Asn-144 for His-144 was found in this new allelic variant of MLC1-V/sB.
ISSN:	0006-2928 1573-4927
DOI:	10.1007/BF00557334