Fourier transform infrared study of the primary electron donor in chromatophores of Rhodobacter sphaeroides with reaction centers genetically modified at residues M160 and L131

Structural changes in chromatophores of Rhodobacter sphaeroides reaction center mutants associated with the substitution of amino acid residues near the primary electron donor P have been investigated by light-induced FTIR difference spectroscopy. The single-site mutations Leu-L131 to His and Leu-M1...

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Veröffentlicht in:Biochemistry (Easton) 1993-12, Vol.32 (50), p.13879-13885
Hauptverfasser: Nabedryk, E, Allen, J. P, Taguchi, A. K. W, Williams, J. C, Woodbury, N. W, Breton, J
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container_issue 50
container_start_page 13879
container_title Biochemistry (Easton)
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creator Nabedryk, E
Allen, J. P
Taguchi, A. K. W
Williams, J. C
Woodbury, N. W
Breton, J
description Structural changes in chromatophores of Rhodobacter sphaeroides reaction center mutants associated with the substitution of amino acid residues near the primary electron donor P have been investigated by light-induced FTIR difference spectroscopy. The single-site mutations Leu-L131 to His and Leu-M160 to His and the corresponding double mutation were designed to introduce a proton-donating residue that could form a hydrogen bond with the keto carbonyl of ring V of each bacteriochlorophyll (PL and PM) of the dimer. The presence of large positive bands at approximately 1550, 1480, and 1295 cm-1, as well as at 2600-2800 cm-1 in the light-induced P+QA-/PQA FTIR difference spectra, corresponding to the photooxidation of P and the photoreduction of the primary quinone QA, demonstrates that the BChl dimer state of P+ is preserved in the LH(L131), LH(M160), and LH(M160)+LH(L131) mutants, although frequency shifts and amplitude changes can be observed, notably for LH(M160). Compared to wild type, these changes are thought to reflect a different charge repartition over the two BChls in P+. Large frequency downshifts in the 9-keto C=O stretching region of the P+QA-/PQA FTIR difference spectra of chromatophores are observed in the mutant samples relative to wild type. For the LH(M160) mutant, a large differential signal at 1678/1664 cm-1 is assigned to a shift, upon photooxidation, of the 9-keto C=O of PM hydrogen-bonded to His-M160, while that at 1718/1696 cm-1 corresponds to the free 9-keto C=O of PL.
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The presence of large positive bands at approximately 1550, 1480, and 1295 cm-1, as well as at 2600-2800 cm-1 in the light-induced P+QA-/PQA FTIR difference spectra, corresponding to the photooxidation of P and the photoreduction of the primary quinone QA, demonstrates that the BChl dimer state of P+ is preserved in the LH(L131), LH(M160), and LH(M160)+LH(L131) mutants, although frequency shifts and amplitude changes can be observed, notably for LH(M160). Compared to wild type, these changes are thought to reflect a different charge repartition over the two BChls in P+. Large frequency downshifts in the 9-keto C=O stretching region of the P+QA-/PQA FTIR difference spectra of chromatophores are observed in the mutant samples relative to wild type. 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Models</topic><topic>Molecular biophysics</topic><topic>Mutation</topic><topic>Photosynthetic Reaction Center Complex Proteins - chemistry</topic><topic>Photosynthetic Reaction Center Complex Proteins - genetics</topic><topic>Physical chemistry in biology</topic><topic>Rhodobacter sphaeroides - chemistry</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nabedryk, E</creatorcontrib><creatorcontrib>Allen, J. P</creatorcontrib><creatorcontrib>Taguchi, A. K. W</creatorcontrib><creatorcontrib>Williams, J. C</creatorcontrib><creatorcontrib>Woodbury, N. 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W</au><au>Breton, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fourier transform infrared study of the primary electron donor in chromatophores of Rhodobacter sphaeroides with reaction centers genetically modified at residues M160 and L131</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1993-12-01</date><risdate>1993</risdate><volume>32</volume><issue>50</issue><spage>13879</spage><epage>13885</epage><pages>13879-13885</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>Structural changes in chromatophores of Rhodobacter sphaeroides reaction center mutants associated with the substitution of amino acid residues near the primary electron donor P have been investigated by light-induced FTIR difference spectroscopy. The single-site mutations Leu-L131 to His and Leu-M160 to His and the corresponding double mutation were designed to introduce a proton-donating residue that could form a hydrogen bond with the keto carbonyl of ring V of each bacteriochlorophyll (PL and PM) of the dimer. The presence of large positive bands at approximately 1550, 1480, and 1295 cm-1, as well as at 2600-2800 cm-1 in the light-induced P+QA-/PQA FTIR difference spectra, corresponding to the photooxidation of P and the photoreduction of the primary quinone QA, demonstrates that the BChl dimer state of P+ is preserved in the LH(L131), LH(M160), and LH(M160)+LH(L131) mutants, although frequency shifts and amplitude changes can be observed, notably for LH(M160). Compared to wild type, these changes are thought to reflect a different charge repartition over the two BChls in P+. Large frequency downshifts in the 9-keto C=O stretching region of the P+QA-/PQA FTIR difference spectra of chromatophores are observed in the mutant samples relative to wild type. For the LH(M160) mutant, a large differential signal at 1678/1664 cm-1 is assigned to a shift, upon photooxidation, of the 9-keto C=O of PM hydrogen-bonded to His-M160, while that at 1718/1696 cm-1 corresponds to the free 9-keto C=O of PL.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>8268163</pmid><doi>10.1021/bi00213a017</doi><tpages>7</tpages></addata></record>
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source MEDLINE; American Chemical Society Journals
subjects Bacterial Chromatophores - chemistry
Biological and medical sciences
Electrons
Fundamental and applied biological sciences. Psychology
Hydrogen Bonding
Light-Harvesting Protein Complexes
Mechanisms. Catalysis. Electron transfer. Models
Molecular biophysics
Mutation
Photosynthetic Reaction Center Complex Proteins - chemistry
Photosynthetic Reaction Center Complex Proteins - genetics
Physical chemistry in biology
Rhodobacter sphaeroides - chemistry
Spectroscopy, Fourier Transform Infrared
title Fourier transform infrared study of the primary electron donor in chromatophores of Rhodobacter sphaeroides with reaction centers genetically modified at residues M160 and L131
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