Isolation of an Antibacterial Peptide from Human Lung Lavage Fluid
The contribution of extracellular secretions to the antibacterial defenses of the lungs remains poorly defined. Recent studies have demonstrated that mouse and rabbit bronchoalveolar washings contain a low-molecular-weight peptide that has antibacterial activity against Escherichia coli. In this stu...
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| Veröffentlicht in: | The Journal of infectious diseases 1985-06, Vol.151 (6), p.1123-1129 |
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| container_end_page | 1129 |
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| container_title | The Journal of infectious diseases |
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| creator | Ellison, Richard T. Boose, Dorothy LaForce, F. Marc |
| description | The contribution of extracellular secretions to the antibacterial defenses of the lungs remains poorly defined. Recent studies have demonstrated that mouse and rabbit bronchoalveolar washings contain a low-molecular-weight peptide that has antibacterial activity against Escherichia coli. In this study we investigated whether a similar peptide could be identified in human secretions. Bronchoalveolar lavage fluid was obtained from normal volunteers and patients with interstitial lung disease or pulmonary alveolar proteinosis. Cellular material and surfactant lipids were removed from the fluid by sequential centrifugations, and the supernatant was fractionated by exclusion filtration to isolate peptides with a molecular weight |
| doi_str_mv | 10.1093/infdis/151.6.1123 |
| format | Article |
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Marc</creator><creatorcontrib>Ellison, Richard T. ; Boose, Dorothy ; LaForce, F. Marc</creatorcontrib><description>The contribution of extracellular secretions to the antibacterial defenses of the lungs remains poorly defined. Recent studies have demonstrated that mouse and rabbit bronchoalveolar washings contain a low-molecular-weight peptide that has antibacterial activity against Escherichia coli. In this study we investigated whether a similar peptide could be identified in human secretions. Bronchoalveolar lavage fluid was obtained from normal volunteers and patients with interstitial lung disease or pulmonary alveolar proteinosis. Cellular material and surfactant lipids were removed from the fluid by sequential centrifugations, and the supernatant was fractionated by exclusion filtration to isolate peptides with a molecular weight <10,000. Gel filtration chromatography separated the ultrafiltrate into several peaks, the first of which had antibacterial activity against E. coli. This material was further separated into several hydrophilic peaks by reverse-phase high-pressure liquid chromatography (RPHPLC). All samples had similar RPHPLC graphs. 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Marc</creatorcontrib><title>Isolation of an Antibacterial Peptide from Human Lung Lavage Fluid</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>The contribution of extracellular secretions to the antibacterial defenses of the lungs remains poorly defined. Recent studies have demonstrated that mouse and rabbit bronchoalveolar washings contain a low-molecular-weight peptide that has antibacterial activity against Escherichia coli. In this study we investigated whether a similar peptide could be identified in human secretions. Bronchoalveolar lavage fluid was obtained from normal volunteers and patients with interstitial lung disease or pulmonary alveolar proteinosis. Cellular material and surfactant lipids were removed from the fluid by sequential centrifugations, and the supernatant was fractionated by exclusion filtration to isolate peptides with a molecular weight <10,000. Gel filtration chromatography separated the ultrafiltrate into several peaks, the first of which had antibacterial activity against E. coli. This material was further separated into several hydrophilic peaks by reverse-phase high-pressure liquid chromatography (RPHPLC). All samples had similar RPHPLC graphs. Material from the third RPHPLC peak produced an antibacterial effect similar to that produced by the rabbit and mouse peptide.</description><subject>Alveolar macrophages</subject><subject>Anti-Bacterial Agents - isolation & purification</subject><subject>Antibacterials</subject><subject>Biological and medical sciences</subject><subject>Bronchoalveolar lavage</subject><subject>Chromatography, Gel</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Edetic Acid - pharmacology</subject><subject>Escherichia coli</subject><subject>Escherichia coli - drug effects</subject><subject>Gels</subject><subject>Hot Temperature</subject><subject>Humans</subject><subject>Lung - analysis</subject><subject>Lung - immunology</subject><subject>Lungs</subject><subject>Medical sciences</subject><subject>Molecular Weight</subject><subject>Original Articles</subject><subject>Penicillin</subject><subject>Peptides - isolation & purification</subject><subject>Phosphates</subject><subject>Phosphates - pharmacology</subject><subject>Pneumology</subject><subject>Pulmonary alveoli</subject><subject>Respiratory system : syndromes and miscellaneous diseases</subject><subject>Therapeutic irrigation</subject><subject>Zinc</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE9PHCEYh4nR2K3tB_DQZA7G26y8wMByVON2TcfowSaNF_LuAAadPyvMmPbbF7NbPfbE4Xl4Aj9CjoHOgWp-FnpvQzqDCuZyDsD4HplBxVUpJfB9MqOUsRIWWn8in1N6opQKLtUhOeSaceB0Ri6u09DiGIa-GHyBfXHej2GNzehiwLa4c5sxWFf4OHTFauqyUE_9Y1HjKz66YtlOwX4hBx7b5L7uziPyc3l1f7kq69vv15fnddkIkGNpPVt7WHu06DVCJZi0WvmFkFL5ii4qb5Er62h-m-JMZahZI7IsG8guPyKn2-4mDi-TS6PpQmpc22LvhikZJYEJKhb_FUHkr0slswhbsYlDStF5s4mhw_jHADVvA5vtwCYPbKR5Gzjf-baLT-vO2fcbu0UzP9lxTA22PmLf5MI_TTMJSsNH5imNQ_yo0NygWmRebnlIo_v9zjE-G6m4qszq14PR_OG-vvhxY5b8LweOnCA</recordid><startdate>19850601</startdate><enddate>19850601</enddate><creator>Ellison, Richard T.</creator><creator>Boose, Dorothy</creator><creator>LaForce, F. Marc</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>19850601</creationdate><title>Isolation of an Antibacterial Peptide from Human Lung Lavage Fluid</title><author>Ellison, Richard T. ; Boose, Dorothy ; LaForce, F. Marc</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c416t-df2bf1bfadaf9a15426d97f84667f5085fda37de03927327d9792c4af96c126d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Alveolar macrophages</topic><topic>Anti-Bacterial Agents - isolation & purification</topic><topic>Antibacterials</topic><topic>Biological and medical sciences</topic><topic>Bronchoalveolar lavage</topic><topic>Chromatography, Gel</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Edetic Acid - pharmacology</topic><topic>Escherichia coli</topic><topic>Escherichia coli - drug effects</topic><topic>Gels</topic><topic>Hot Temperature</topic><topic>Humans</topic><topic>Lung - analysis</topic><topic>Lung - immunology</topic><topic>Lungs</topic><topic>Medical sciences</topic><topic>Molecular Weight</topic><topic>Original Articles</topic><topic>Penicillin</topic><topic>Peptides - isolation & purification</topic><topic>Phosphates</topic><topic>Phosphates - pharmacology</topic><topic>Pneumology</topic><topic>Pulmonary alveoli</topic><topic>Respiratory system : syndromes and miscellaneous diseases</topic><topic>Therapeutic irrigation</topic><topic>Zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ellison, Richard T.</creatorcontrib><creatorcontrib>Boose, Dorothy</creatorcontrib><creatorcontrib>LaForce, F. Marc</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ellison, Richard T.</au><au>Boose, Dorothy</au><au>LaForce, F. Marc</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of an Antibacterial Peptide from Human Lung Lavage Fluid</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1985-06-01</date><risdate>1985</risdate><volume>151</volume><issue>6</issue><spage>1123</spage><epage>1129</epage><pages>1123-1129</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>The contribution of extracellular secretions to the antibacterial defenses of the lungs remains poorly defined. Recent studies have demonstrated that mouse and rabbit bronchoalveolar washings contain a low-molecular-weight peptide that has antibacterial activity against Escherichia coli. In this study we investigated whether a similar peptide could be identified in human secretions. Bronchoalveolar lavage fluid was obtained from normal volunteers and patients with interstitial lung disease or pulmonary alveolar proteinosis. Cellular material and surfactant lipids were removed from the fluid by sequential centrifugations, and the supernatant was fractionated by exclusion filtration to isolate peptides with a molecular weight <10,000. Gel filtration chromatography separated the ultrafiltrate into several peaks, the first of which had antibacterial activity against E. coli. This material was further separated into several hydrophilic peaks by reverse-phase high-pressure liquid chromatography (RPHPLC). All samples had similar RPHPLC graphs. Material from the third RPHPLC peak produced an antibacterial effect similar to that produced by the rabbit and mouse peptide.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>3923130</pmid><doi>10.1093/infdis/151.6.1123</doi><tpages>7</tpages></addata></record> |
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| source | Jstor Complete Legacy; MEDLINE |
| subjects | Alveolar macrophages Anti-Bacterial Agents - isolation & purification Antibacterials Biological and medical sciences Bronchoalveolar lavage Chromatography, Gel Chromatography, High Pressure Liquid Edetic Acid - pharmacology Escherichia coli Escherichia coli - drug effects Gels Hot Temperature Humans Lung - analysis Lung - immunology Lungs Medical sciences Molecular Weight Original Articles Penicillin Peptides - isolation & purification Phosphates Phosphates - pharmacology Pneumology Pulmonary alveoli Respiratory system : syndromes and miscellaneous diseases Therapeutic irrigation Zinc |
| title | Isolation of an Antibacterial Peptide from Human Lung Lavage Fluid |
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