Preparation and characterization of antisera and monoclonal antibodies to serotonergic and dopaminergic ligands
In an attempt to produce polyclonal antisera and monoclonal antibodies to serotonin, SKF 38393 (D-1 agonist), dopamine, and haloperidol (D-2 antagonist) several procedures for the preparation of immunogenic ligand-protein carrier conjugates were investigated. The Mannich reaction utilizing formaldeh...
Gespeichert in:
| Veröffentlicht in: | Journal of neuroimmunology 1985-01, Vol.8 (2-3), p.115-127 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 127 |
|---|---|
| container_issue | 2-3 |
| container_start_page | 115 |
| container_title | Journal of neuroimmunology |
| container_volume | 8 |
| creator | Flurkey, K. Bolger, M.B. Linthicum, D.S. |
| description | In an attempt to produce polyclonal antisera and monoclonal antibodies to serotonin, SKF 38393 (D-1 agonist), dopamine, and haloperidol (D-2 antagonist) several procedures for the preparation of immunogenic ligand-protein carrier conjugates were investigated. The Mannich reaction utilizing formaldehyde as the chemical linker was used to prepare serotonin-protein conjugates; antibodies raised to this conjugate reacted specifically to the conjugated serotonin moiety but did not react to native serotonin. Chemical conjugations involving dimethylpimelylimidate or N-carboxymethyl derivatives for the coupling of serotonin, dopamine and SKF 38393 to carrier proteins produced antibodies primarily directed against the ‘chemical coupling arm’ and very little antibody activity against the ligand itself could be detected. Synthesis of a haloperidol derivative suitable for chemical coupling to a protein carrier via oxobutyric acid produced an immunogen which was capable of eliciting both polyclonal and monoclonal antibodies specific for the hapten. The pitfalls of the various chemical conjugation procedures and the difficulties of producing antibodies to free ligands are discussed. |
| doi_str_mv | 10.1016/S0165-5728(85)80052-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_76121965</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0165572885800527</els_id><sourcerecordid>76121965</sourcerecordid><originalsourceid>FETCH-LOGICAL-c420t-60812ae0cce95280c394496a1d0c6305799592f2b137d5fc7529ac6e36b307893</originalsourceid><addsrcrecordid>eNqFkU1r3DAQhkVo2W4-fsLCHkpJDk71YX2dSglJWgg0kOQstONxqmJbW8kbaH59Fa_Zay4a9M4zI828hKwYvWSUqa8P5ZCV1NycG3lhKJW80kdkyYzmlak5-0CWB-QTOc75D6VMitouyEIYLWrNlyTeJ9z65McQh7UfmjX8LjcYMYXXvRjboo8hY_IT0MchQhcH3036JjYB83qM60LEMQ6YngNMZBO3vg-z0IXnouVT8rH1XcazOZ6Qp5vrx6sf1d2v259X3-8qqDkdK0UN4x4pAFrJDQVh69oqzxoKSlCprZWWt3zDhG5kC1py60GhUBtBtbHihHzZ992m-HeHeXR9yIBd5weMu-y0YpxZJd8FWc0ss0IVUO5BSDHnhK3bptD79M8x6t4ccZMj7m3dzkg3OeJ0qVvND-w2PTaHqtmCkv88530G37XJDxDyAbNMcjUN9G2PYdnaS8DkMgQcAJuQEEbXxPDOR_4Dp86oqQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14191936</pqid></control><display><type>article</type><title>Preparation and characterization of antisera and monoclonal antibodies to serotonergic and dopaminergic ligands</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Flurkey, K. ; Bolger, M.B. ; Linthicum, D.S.</creator><creatorcontrib>Flurkey, K. ; Bolger, M.B. ; Linthicum, D.S.</creatorcontrib><description>In an attempt to produce polyclonal antisera and monoclonal antibodies to serotonin, SKF 38393 (D-1 agonist), dopamine, and haloperidol (D-2 antagonist) several procedures for the preparation of immunogenic ligand-protein carrier conjugates were investigated. The Mannich reaction utilizing formaldehyde as the chemical linker was used to prepare serotonin-protein conjugates; antibodies raised to this conjugate reacted specifically to the conjugated serotonin moiety but did not react to native serotonin. Chemical conjugations involving dimethylpimelylimidate or N-carboxymethyl derivatives for the coupling of serotonin, dopamine and SKF 38393 to carrier proteins produced antibodies primarily directed against the ‘chemical coupling arm’ and very little antibody activity against the ligand itself could be detected. Synthesis of a haloperidol derivative suitable for chemical coupling to a protein carrier via oxobutyric acid produced an immunogen which was capable of eliciting both polyclonal and monoclonal antibodies specific for the hapten. The pitfalls of the various chemical conjugation procedures and the difficulties of producing antibodies to free ligands are discussed.</description><identifier>ISSN: 0165-5728</identifier><identifier>EISSN: 1872-8421</identifier><identifier>DOI: 10.1016/S0165-5728(85)80052-7</identifier><identifier>PMID: 3873472</identifier><identifier>CODEN: JNRIDW</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine ; Animals ; Antibodies - immunology ; Antibodies, Monoclonal - immunology ; Antisera ; Benzazepines - immunology ; Biological and medical sciences ; Biotechnology ; Dopamine ; Dopamine - immunology ; Fundamental and applied biological sciences. Psychology ; Haloperidol ; Haloperidol - immunology ; Haptens - immunology ; Health. Pharmaceutical industry ; Immunochemistry ; Industrial applications and implications. Economical aspects ; Ligands ; Medical sciences ; Mice ; Monoclonal antibodies ; Neuropharmacology ; Neurotransmitters. Neurotransmission. Receptors ; Pharmacology. Drug treatments ; Production of active biomolecules ; Rabbits ; Serotonin ; Serotonin - immunology</subject><ispartof>Journal of neuroimmunology, 1985-01, Vol.8 (2-3), p.115-127</ispartof><rights>1985 Elsevier Science Publishers (Biomedical Division) All rights reserved</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-60812ae0cce95280c394496a1d0c6305799592f2b137d5fc7529ac6e36b307893</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0165-5728(85)80052-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9152689$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3873472$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Flurkey, K.</creatorcontrib><creatorcontrib>Bolger, M.B.</creatorcontrib><creatorcontrib>Linthicum, D.S.</creatorcontrib><title>Preparation and characterization of antisera and monoclonal antibodies to serotonergic and dopaminergic ligands</title><title>Journal of neuroimmunology</title><addtitle>J Neuroimmunol</addtitle><description>In an attempt to produce polyclonal antisera and monoclonal antibodies to serotonin, SKF 38393 (D-1 agonist), dopamine, and haloperidol (D-2 antagonist) several procedures for the preparation of immunogenic ligand-protein carrier conjugates were investigated. The Mannich reaction utilizing formaldehyde as the chemical linker was used to prepare serotonin-protein conjugates; antibodies raised to this conjugate reacted specifically to the conjugated serotonin moiety but did not react to native serotonin. Chemical conjugations involving dimethylpimelylimidate or N-carboxymethyl derivatives for the coupling of serotonin, dopamine and SKF 38393 to carrier proteins produced antibodies primarily directed against the ‘chemical coupling arm’ and very little antibody activity against the ligand itself could be detected. Synthesis of a haloperidol derivative suitable for chemical coupling to a protein carrier via oxobutyric acid produced an immunogen which was capable of eliciting both polyclonal and monoclonal antibodies specific for the hapten. The pitfalls of the various chemical conjugation procedures and the difficulties of producing antibodies to free ligands are discussed.</description><subject>2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine</subject><subject>Animals</subject><subject>Antibodies - immunology</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antisera</subject><subject>Benzazepines - immunology</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Dopamine</subject><subject>Dopamine - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Haloperidol</subject><subject>Haloperidol - immunology</subject><subject>Haptens - immunology</subject><subject>Health. Pharmaceutical industry</subject><subject>Immunochemistry</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Ligands</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Monoclonal antibodies</subject><subject>Neuropharmacology</subject><subject>Neurotransmitters. Neurotransmission. Receptors</subject><subject>Pharmacology. Drug treatments</subject><subject>Production of active biomolecules</subject><subject>Rabbits</subject><subject>Serotonin</subject><subject>Serotonin - immunology</subject><issn>0165-5728</issn><issn>1872-8421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVo2W4-fsLCHkpJDk71YX2dSglJWgg0kOQstONxqmJbW8kbaH59Fa_Zay4a9M4zI828hKwYvWSUqa8P5ZCV1NycG3lhKJW80kdkyYzmlak5-0CWB-QTOc75D6VMitouyEIYLWrNlyTeJ9z65McQh7UfmjX8LjcYMYXXvRjboo8hY_IT0MchQhcH3036JjYB83qM60LEMQ6YngNMZBO3vg-z0IXnouVT8rH1XcazOZ6Qp5vrx6sf1d2v259X3-8qqDkdK0UN4x4pAFrJDQVh69oqzxoKSlCprZWWt3zDhG5kC1py60GhUBtBtbHihHzZ992m-HeHeXR9yIBd5weMu-y0YpxZJd8FWc0ss0IVUO5BSDHnhK3bptD79M8x6t4ccZMj7m3dzkg3OeJ0qVvND-w2PTaHqtmCkv88530G37XJDxDyAbNMcjUN9G2PYdnaS8DkMgQcAJuQEEbXxPDOR_4Dp86oqQ</recordid><startdate>19850101</startdate><enddate>19850101</enddate><creator>Flurkey, K.</creator><creator>Bolger, M.B.</creator><creator>Linthicum, D.S.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>19850101</creationdate><title>Preparation and characterization of antisera and monoclonal antibodies to serotonergic and dopaminergic ligands</title><author>Flurkey, K. ; Bolger, M.B. ; Linthicum, D.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-60812ae0cce95280c394496a1d0c6305799592f2b137d5fc7529ac6e36b307893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine</topic><topic>Animals</topic><topic>Antibodies - immunology</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antisera</topic><topic>Benzazepines - immunology</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Dopamine</topic><topic>Dopamine - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Haloperidol</topic><topic>Haloperidol - immunology</topic><topic>Haptens - immunology</topic><topic>Health. Pharmaceutical industry</topic><topic>Immunochemistry</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Ligands</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Monoclonal antibodies</topic><topic>Neuropharmacology</topic><topic>Neurotransmitters. Neurotransmission. Receptors</topic><topic>Pharmacology. Drug treatments</topic><topic>Production of active biomolecules</topic><topic>Rabbits</topic><topic>Serotonin</topic><topic>Serotonin - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Flurkey, K.</creatorcontrib><creatorcontrib>Bolger, M.B.</creatorcontrib><creatorcontrib>Linthicum, D.S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroimmunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Flurkey, K.</au><au>Bolger, M.B.</au><au>Linthicum, D.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation and characterization of antisera and monoclonal antibodies to serotonergic and dopaminergic ligands</atitle><jtitle>Journal of neuroimmunology</jtitle><addtitle>J Neuroimmunol</addtitle><date>1985-01-01</date><risdate>1985</risdate><volume>8</volume><issue>2-3</issue><spage>115</spage><epage>127</epage><pages>115-127</pages><issn>0165-5728</issn><eissn>1872-8421</eissn><coden>JNRIDW</coden><abstract>In an attempt to produce polyclonal antisera and monoclonal antibodies to serotonin, SKF 38393 (D-1 agonist), dopamine, and haloperidol (D-2 antagonist) several procedures for the preparation of immunogenic ligand-protein carrier conjugates were investigated. The Mannich reaction utilizing formaldehyde as the chemical linker was used to prepare serotonin-protein conjugates; antibodies raised to this conjugate reacted specifically to the conjugated serotonin moiety but did not react to native serotonin. Chemical conjugations involving dimethylpimelylimidate or N-carboxymethyl derivatives for the coupling of serotonin, dopamine and SKF 38393 to carrier proteins produced antibodies primarily directed against the ‘chemical coupling arm’ and very little antibody activity against the ligand itself could be detected. Synthesis of a haloperidol derivative suitable for chemical coupling to a protein carrier via oxobutyric acid produced an immunogen which was capable of eliciting both polyclonal and monoclonal antibodies specific for the hapten. The pitfalls of the various chemical conjugation procedures and the difficulties of producing antibodies to free ligands are discussed.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>3873472</pmid><doi>10.1016/S0165-5728(85)80052-7</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-5728 |
| ispartof | Journal of neuroimmunology, 1985-01, Vol.8 (2-3), p.115-127 |
| issn | 0165-5728 1872-8421 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76121965 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine Animals Antibodies - immunology Antibodies, Monoclonal - immunology Antisera Benzazepines - immunology Biological and medical sciences Biotechnology Dopamine Dopamine - immunology Fundamental and applied biological sciences. Psychology Haloperidol Haloperidol - immunology Haptens - immunology Health. Pharmaceutical industry Immunochemistry Industrial applications and implications. Economical aspects Ligands Medical sciences Mice Monoclonal antibodies Neuropharmacology Neurotransmitters. Neurotransmission. Receptors Pharmacology. Drug treatments Production of active biomolecules Rabbits Serotonin Serotonin - immunology |
| title | Preparation and characterization of antisera and monoclonal antibodies to serotonergic and dopaminergic ligands |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T18%3A25%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Preparation%20and%20characterization%20of%20antisera%20and%20monoclonal%20antibodies%20to%20serotonergic%20and%20dopaminergic%20ligands&rft.jtitle=Journal%20of%20neuroimmunology&rft.au=Flurkey,%20K.&rft.date=1985-01-01&rft.volume=8&rft.issue=2-3&rft.spage=115&rft.epage=127&rft.pages=115-127&rft.issn=0165-5728&rft.eissn=1872-8421&rft.coden=JNRIDW&rft_id=info:doi/10.1016/S0165-5728(85)80052-7&rft_dat=%3Cproquest_cross%3E76121965%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14191936&rft_id=info:pmid/3873472&rft_els_id=S0165572885800527&rfr_iscdi=true |