Angiotensin Increases Inositol Trisphosphate and Calcium in Vascular Smooth Muscle
Angiotensin II stimulated the breakdown of phosphatidylinositol-4,5-bisphosphate (PIP2) and the generation of inositol trisphosphate (IP3) in cultured rat aortic smooth muscle cells. The decrease in PIP2 and increase in IP, levels were rapid (measurable at 5 seconds; maximum IP, levels at 15 seconds...
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| Veröffentlicht in: | Hypertension (Dallas, Tex. 1979) Tex. 1979), 1985-05, Vol.7 (3, Part 1), p.447-451 |
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| container_end_page | 451 |
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| container_issue | 3, Part 1 |
| container_start_page | 447 |
| container_title | Hypertension (Dallas, Tex. 1979) |
| container_volume | 7 |
| creator | ALEXANDER, R WAYNE BROCK, TOMMY A GIMBRONE, MICHAEL A RITTENHOUSE, SUSAN E |
| description | Angiotensin II stimulated the breakdown of phosphatidylinositol-4,5-bisphosphate (PIP2) and the generation of inositol trisphosphate (IP3) in cultured rat aortic smooth muscle cells. The decrease in PIP2 and increase in IP, levels were rapid (measurable at 5 seconds; maximum IP, levels at 15 seconds). The time course of these changes was comparable to that of angiotensin Il-induced increases in cytosolic free calcium, as measured by the calcium-sensitive fluorescent indicator quin 2. The IP3 formation was not stimulated by the calcium ionophore A23187 (5 μM), nor were angiotensin Il-induced changes in IP, formation inhibited by the removal of extracellular calcium with EGTA. Angiotensin II appears to be capable of generating more IP, than is required for maximal release of intracellular calcium. These data are consistent with the hypothesis that generation of IP3 plays a role in the angiotensin Il-induced mobilization of calcium from intracellular storage sites in vascular smooth muscle cells. |
| doi_str_mv | 10.1161/01.hyp.7.3.447 |
| format | Article |
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The decrease in PIP2 and increase in IP, levels were rapid (measurable at 5 seconds; maximum IP, levels at 15 seconds). The time course of these changes was comparable to that of angiotensin Il-induced increases in cytosolic free calcium, as measured by the calcium-sensitive fluorescent indicator quin 2. The IP3 formation was not stimulated by the calcium ionophore A23187 (5 μM), nor were angiotensin Il-induced changes in IP, formation inhibited by the removal of extracellular calcium with EGTA. Angiotensin II appears to be capable of generating more IP, than is required for maximal release of intracellular calcium. These data are consistent with the hypothesis that generation of IP3 plays a role in the angiotensin Il-induced mobilization of calcium from intracellular storage sites in vascular smooth muscle cells.</description><identifier>ISSN: 0194-911X</identifier><identifier>EISSN: 1524-4563</identifier><identifier>DOI: 10.1161/01.hyp.7.3.447</identifier><identifier>PMID: 2987120</identifier><identifier>CODEN: HPRTDN</identifier><language>eng</language><publisher>Philadelphia, PA: American Heart Association, Inc</publisher><subject>Angiotensin II - pharmacology ; Animals ; Aorta, Thoracic - cytology ; Arterial hypertension. Arterial hypotension ; Biological and medical sciences ; Blood and lymphatic vessels ; Calcium - analysis ; Carbon Radioisotopes ; Cardiology. Vascular system ; Cells, Cultured ; Experimental diseases ; Extracellular Space - analysis ; Inositol 1,4,5-Trisphosphate ; Inositol Phosphates - biosynthesis ; Medical sciences ; Muscle, Smooth, Vascular - drug effects ; Muscle, Smooth, Vascular - metabolism ; Phosphatidylinositol 4,5-Diphosphate ; Phosphatidylinositols - metabolism ; Rats ; Sugar Phosphates - biosynthesis</subject><ispartof>Hypertension (Dallas, Tex. 1979), 1985-05, Vol.7 (3, Part 1), p.447-451</ispartof><rights>1985 American Heart Association, Inc.</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4422-a541864222d71da6c668559167044f5d5a5ff0254a4e1efcb3410b07f9bb4c2a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3687,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8499260$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2987120$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>ALEXANDER, R WAYNE</creatorcontrib><creatorcontrib>BROCK, TOMMY A</creatorcontrib><creatorcontrib>GIMBRONE, MICHAEL A</creatorcontrib><creatorcontrib>RITTENHOUSE, SUSAN E</creatorcontrib><title>Angiotensin Increases Inositol Trisphosphate and Calcium in Vascular Smooth Muscle</title><title>Hypertension (Dallas, Tex. 1979)</title><addtitle>Hypertension</addtitle><description>Angiotensin II stimulated the breakdown of phosphatidylinositol-4,5-bisphosphate (PIP2) and the generation of inositol trisphosphate (IP3) in cultured rat aortic smooth muscle cells. The decrease in PIP2 and increase in IP, levels were rapid (measurable at 5 seconds; maximum IP, levels at 15 seconds). The time course of these changes was comparable to that of angiotensin Il-induced increases in cytosolic free calcium, as measured by the calcium-sensitive fluorescent indicator quin 2. The IP3 formation was not stimulated by the calcium ionophore A23187 (5 μM), nor were angiotensin Il-induced changes in IP, formation inhibited by the removal of extracellular calcium with EGTA. Angiotensin II appears to be capable of generating more IP, than is required for maximal release of intracellular calcium. These data are consistent with the hypothesis that generation of IP3 plays a role in the angiotensin Il-induced mobilization of calcium from intracellular storage sites in vascular smooth muscle cells.</description><subject>Angiotensin II - pharmacology</subject><subject>Animals</subject><subject>Aorta, Thoracic - cytology</subject><subject>Arterial hypertension. Arterial hypotension</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Calcium - analysis</subject><subject>Carbon Radioisotopes</subject><subject>Cardiology. Vascular system</subject><subject>Cells, Cultured</subject><subject>Experimental diseases</subject><subject>Extracellular Space - analysis</subject><subject>Inositol 1,4,5-Trisphosphate</subject><subject>Inositol Phosphates - biosynthesis</subject><subject>Medical sciences</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Phosphatidylinositol 4,5-Diphosphate</subject><subject>Phosphatidylinositols - metabolism</subject><subject>Rats</subject><subject>Sugar Phosphates - biosynthesis</subject><issn>0194-911X</issn><issn>1524-4563</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMFr3TAMxs3oaN_aXXcr5FB2S2Y5spMcy2NbCx0baze2k3EcZ0nrxK9WQul_P5f3qEDoE_pJoI-xD8ALAAWfOBTD866oirJArN6wDUiBOUpVHrENhwbzBuDPCXtHdM85YIKO2bFo6goE37Cfl_O_MSxupnHOrmcbnSFHSQUal-CzuzjSbggpzeIyM3fZ1ng7rlOW-N-G7OpNzG6nEJYh-7aS9e6Mve2NJ_f-UE_Zry-f77ZX-c33r9fby5vcIgqRG4lQq6REV0FnlFWqlrIBVXHEXnbSyL7nQqJBB663bYnAW171TduiFaY8ZR_3d3cxPK6OFj2NZJ33ZnZhJV2p9KFATGCxB20MRNH1ehfHycRnDVy_mKg56Ku_P3SlS538SQvnh8trO7nuFT-4luYXh3kywPg-mtmO9IrV2DRCvWC4x56CX1ykB78-uagHZ_wyaJ4ChapzaGrJZerylEKU_wGtBIlT</recordid><startdate>198505</startdate><enddate>198505</enddate><creator>ALEXANDER, R WAYNE</creator><creator>BROCK, TOMMY A</creator><creator>GIMBRONE, MICHAEL A</creator><creator>RITTENHOUSE, SUSAN E</creator><general>American Heart Association, Inc</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198505</creationdate><title>Angiotensin Increases Inositol Trisphosphate and Calcium in Vascular Smooth Muscle</title><author>ALEXANDER, R WAYNE ; BROCK, TOMMY A ; GIMBRONE, MICHAEL A ; RITTENHOUSE, SUSAN E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4422-a541864222d71da6c668559167044f5d5a5ff0254a4e1efcb3410b07f9bb4c2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Angiotensin II - pharmacology</topic><topic>Animals</topic><topic>Aorta, Thoracic - cytology</topic><topic>Arterial hypertension. Arterial hypotension</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Calcium - analysis</topic><topic>Carbon Radioisotopes</topic><topic>Cardiology. Vascular system</topic><topic>Cells, Cultured</topic><topic>Experimental diseases</topic><topic>Extracellular Space - analysis</topic><topic>Inositol 1,4,5-Trisphosphate</topic><topic>Inositol Phosphates - biosynthesis</topic><topic>Medical sciences</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Phosphatidylinositol 4,5-Diphosphate</topic><topic>Phosphatidylinositols - metabolism</topic><topic>Rats</topic><topic>Sugar Phosphates - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>ALEXANDER, R WAYNE</creatorcontrib><creatorcontrib>BROCK, TOMMY A</creatorcontrib><creatorcontrib>GIMBRONE, MICHAEL A</creatorcontrib><creatorcontrib>RITTENHOUSE, SUSAN E</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Hypertension (Dallas, Tex. 1979)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>ALEXANDER, R WAYNE</au><au>BROCK, TOMMY A</au><au>GIMBRONE, MICHAEL A</au><au>RITTENHOUSE, SUSAN E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Angiotensin Increases Inositol Trisphosphate and Calcium in Vascular Smooth Muscle</atitle><jtitle>Hypertension (Dallas, Tex. 1979)</jtitle><addtitle>Hypertension</addtitle><date>1985-05</date><risdate>1985</risdate><volume>7</volume><issue>3, Part 1</issue><spage>447</spage><epage>451</epage><pages>447-451</pages><issn>0194-911X</issn><eissn>1524-4563</eissn><coden>HPRTDN</coden><abstract>Angiotensin II stimulated the breakdown of phosphatidylinositol-4,5-bisphosphate (PIP2) and the generation of inositol trisphosphate (IP3) in cultured rat aortic smooth muscle cells. The decrease in PIP2 and increase in IP, levels were rapid (measurable at 5 seconds; maximum IP, levels at 15 seconds). The time course of these changes was comparable to that of angiotensin Il-induced increases in cytosolic free calcium, as measured by the calcium-sensitive fluorescent indicator quin 2. The IP3 formation was not stimulated by the calcium ionophore A23187 (5 μM), nor were angiotensin Il-induced changes in IP, formation inhibited by the removal of extracellular calcium with EGTA. Angiotensin II appears to be capable of generating more IP, than is required for maximal release of intracellular calcium. These data are consistent with the hypothesis that generation of IP3 plays a role in the angiotensin Il-induced mobilization of calcium from intracellular storage sites in vascular smooth muscle cells.</abstract><cop>Philadelphia, PA</cop><cop>Hagerstown, MD</cop><pub>American Heart Association, Inc</pub><pmid>2987120</pmid><doi>10.1161/01.hyp.7.3.447</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Hypertension (Dallas, Tex. 1979), 1985-05, Vol.7 (3, Part 1), p.447-451 |
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| source | MEDLINE; American Heart Association Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Journals@Ovid Complete |
| subjects | Angiotensin II - pharmacology Animals Aorta, Thoracic - cytology Arterial hypertension. Arterial hypotension Biological and medical sciences Blood and lymphatic vessels Calcium - analysis Carbon Radioisotopes Cardiology. Vascular system Cells, Cultured Experimental diseases Extracellular Space - analysis Inositol 1,4,5-Trisphosphate Inositol Phosphates - biosynthesis Medical sciences Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - metabolism Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositols - metabolism Rats Sugar Phosphates - biosynthesis |
| title | Angiotensin Increases Inositol Trisphosphate and Calcium in Vascular Smooth Muscle |
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