The outer membrane of plant mitochondria contains a calcium-dependent protein kinase and multiple phosphoproteins
Highly purified mitochondria from potato ( Solomon tuberosum L. cv. Bintje) tubers were subfractionated into a matrix fraction, an inner membrane fraction and an outer membrane fraction with minimal cross-contamination. When the matrix and inner membrane fractions were incubated with [γ- 32P]ATP onl...
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| Veröffentlicht in: | FEBS letters 1993-12, Vol.336 (2), p.347-351 |
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| creator | Pical, Christophe Fredlund, Kenneth M. Petit, Patrice X. Sommarin, Marianne Møller, lan M. |
| description | Highly purified mitochondria from potato (
Solomon tuberosum L. cv. Bintje) tubers were subfractionated into a matrix fraction, an inner membrane fraction and an outer membrane fraction with minimal cross-contamination. When the matrix and inner membrane fractions were incubated with [γ-
32P]ATP only one and three prominent phosphoproteins were detected after SDS-PAGE and autoradiography, respectively. In contrast, more than 20 phosphoproteins could be labelled in the outer membrane fraction, the main ones at 12, 18, 26, 43, 58, 60, 65, 74 and 110 kDa. Only one band, at 18 kDa, was detectable when the labelling was done in the presence of EGTA. We conclude that the outer membrane of plant mitochondria contains at least one Ca
2+-dependent protein kinase and more than 20 endogenous substrates. |
| doi_str_mv | 10.1016/0014-5793(93)80835-I |
| format | Article |
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32P]ATP only one and three prominent phosphoproteins were detected after SDS-PAGE and autoradiography, respectively. In contrast, more than 20 phosphoproteins could be labelled in the outer membrane fraction, the main ones at 12, 18, 26, 43, 58, 60, 65, 74 and 110 kDa. Only one band, at 18 kDa, was detectable when the labelling was done in the presence of EGTA. We conclude that the outer membrane of plant mitochondria contains at least one Ca
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Solomon tuberosum L. cv. Bintje) tubers were subfractionated into a matrix fraction, an inner membrane fraction and an outer membrane fraction with minimal cross-contamination. When the matrix and inner membrane fractions were incubated with [γ-
32P]ATP only one and three prominent phosphoproteins were detected after SDS-PAGE and autoradiography, respectively. In contrast, more than 20 phosphoproteins could be labelled in the outer membrane fraction, the main ones at 12, 18, 26, 43, 58, 60, 65, 74 and 110 kDa. Only one band, at 18 kDa, was detectable when the labelling was done in the presence of EGTA. We conclude that the outer membrane of plant mitochondria contains at least one Ca
2+-dependent protein kinase and more than 20 endogenous substrates.</description><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Calcium</subject><subject>CCO, cytochrome c oxidase</subject><subject>CCR, antimycin A-insensitive NADH-cytochrome c reductase</subject><subject>Cell structures and functions</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Inner mitochondrial membrane</subject><subject>Intracellular Membranes - chemistry</subject><subject>MDH, NAD+-dependent malate dehydrogenase</subject><subject>Mitochondria - chemistry</subject><subject>Mitochondria and cell respiration</subject><subject>Molecular and cellular biology</subject><subject>Outer mitochondrial membrane</subject><subject>Phosphoproteins - analysis</subject><subject>Phosphorylation</subject><subject>Plant mitochondrion</subject><subject>Plant Proteins - analysis</subject><subject>Protein Kinases - analysis</subject><subject>Protein phosphorylation</subject><subject>Solanum tuberosum - chemistry</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM-P1CAUx4nRrOPqf6AJB2P0UIVCKVxMdLOjk2ziZe6EwmsGbaELrWb_e-lOM0djAoH33vf9-iD0mpKPlFDxiRDKq6ZV7L1iHySRrKkOT9COypZVjAv5FO0ukufoRc4_SbElVVfoStairgXZofvjCXBcZkh4hLFLJhSzx9NgwoxHP0d7isElb7CNYTY-ZFy-ZrB-GSsHEwQHRTmlOIMP-JcPJgM2weFxGWY_DYCnU8zlbpL8Ej3rzZDh1fZeo-P-9njzvbr78e1w8-WuspyrQ0VBOlBdJ7u-BiV60vScUaEE1LIhvO0ZJ0R2wJlprAMuWFM3xWsZV33H2DV6dy5b-t4vkGc9-mxhKItBXLJuBaWqbmUR8rPQpphzgl5PyY8mPWhK9AparxT1SlGX8whaH0ram63-0o3gLkkb2RJ_u8VNLrz6Qtb6fJExWcqodcz9WfbHD_DwX631_vZrvQZWv2KP3nWez-dCUJj-9pB0th6CBecT2Fm76P-90F9E5q8j</recordid><startdate>19931227</startdate><enddate>19931227</enddate><creator>Pical, Christophe</creator><creator>Fredlund, Kenneth M.</creator><creator>Petit, Patrice X.</creator><creator>Sommarin, Marianne</creator><creator>Møller, lan M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19931227</creationdate><title>The outer membrane of plant mitochondria contains a calcium-dependent protein kinase and multiple phosphoproteins</title><author>Pical, Christophe ; Fredlund, Kenneth M. ; Petit, Patrice X. ; Sommarin, Marianne ; Møller, lan M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c449I-1e8de9bb8bf2e96f05f431696e285047f34008be43a5cde4635257f3c349fb33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Autoradiography</topic><topic>Biological and medical sciences</topic><topic>Calcium</topic><topic>CCO, cytochrome c oxidase</topic><topic>CCR, antimycin A-insensitive NADH-cytochrome c reductase</topic><topic>Cell structures and functions</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Inner mitochondrial membrane</topic><topic>Intracellular Membranes - chemistry</topic><topic>MDH, NAD+-dependent malate dehydrogenase</topic><topic>Mitochondria - chemistry</topic><topic>Mitochondria and cell respiration</topic><topic>Molecular and cellular biology</topic><topic>Outer mitochondrial membrane</topic><topic>Phosphoproteins - analysis</topic><topic>Phosphorylation</topic><topic>Plant mitochondrion</topic><topic>Plant Proteins - analysis</topic><topic>Protein Kinases - analysis</topic><topic>Protein phosphorylation</topic><topic>Solanum tuberosum - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pical, Christophe</creatorcontrib><creatorcontrib>Fredlund, Kenneth M.</creatorcontrib><creatorcontrib>Petit, Patrice X.</creatorcontrib><creatorcontrib>Sommarin, Marianne</creatorcontrib><creatorcontrib>Møller, lan M.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pical, Christophe</au><au>Fredlund, Kenneth M.</au><au>Petit, Patrice X.</au><au>Sommarin, Marianne</au><au>Møller, lan M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The outer membrane of plant mitochondria contains a calcium-dependent protein kinase and multiple phosphoproteins</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1993-12-27</date><risdate>1993</risdate><volume>336</volume><issue>2</issue><spage>347</spage><epage>351</epage><pages>347-351</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>Highly purified mitochondria from potato (
Solomon tuberosum L. cv. Bintje) tubers were subfractionated into a matrix fraction, an inner membrane fraction and an outer membrane fraction with minimal cross-contamination. When the matrix and inner membrane fractions were incubated with [γ-
32P]ATP only one and three prominent phosphoproteins were detected after SDS-PAGE and autoradiography, respectively. In contrast, more than 20 phosphoproteins could be labelled in the outer membrane fraction, the main ones at 12, 18, 26, 43, 58, 60, 65, 74 and 110 kDa. Only one band, at 18 kDa, was detectable when the labelling was done in the presence of EGTA. We conclude that the outer membrane of plant mitochondria contains at least one Ca
2+-dependent protein kinase and more than 20 endogenous substrates.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>8262260</pmid><doi>10.1016/0014-5793(93)80835-I</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | FEBS letters, 1993-12, Vol.336 (2), p.347-351 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76119278 |
| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Autoradiography Biological and medical sciences Calcium CCO, cytochrome c oxidase CCR, antimycin A-insensitive NADH-cytochrome c reductase Cell structures and functions Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Inner mitochondrial membrane Intracellular Membranes - chemistry MDH, NAD+-dependent malate dehydrogenase Mitochondria - chemistry Mitochondria and cell respiration Molecular and cellular biology Outer mitochondrial membrane Phosphoproteins - analysis Phosphorylation Plant mitochondrion Plant Proteins - analysis Protein Kinases - analysis Protein phosphorylation Solanum tuberosum - chemistry |
| title | The outer membrane of plant mitochondria contains a calcium-dependent protein kinase and multiple phosphoproteins |
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