Mechanism-based inactivation of thymine hydroxylase, an .alpha.-ketoglutarate-dependent dioxygenase, by 5-ethynyluracil

5-Ethynyluracil was shown to be a mechanism-based inactivator of thymine 7-hydroxylase, with Ki = 22 micromolar and a k2 = 2.6 min-1. Inactivation resulted in covalent modification of the enzyme with a stoichiometry of approximately 1 adduct/enzyme molecule. The reaction of thymine 7-hydroxylase wit...

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Veröffentlicht in:	Biochemistry (Easton) 1993-12, Vol.32 (50), p.14034-14042
Hauptverfasser:	Thornburg, Lora D, Stubbe, JoAnne
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTIVIDAD ENZIMATICA ACTIVITE ENZYMATIQUE Amino Acid Sequence Analytical, structural and metabolic biochemistry Biological and medical sciences DERIVADOS DEL URACILO DERIVE DE L'URACILE Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology INHIBIDORES DE ENZIMAS INHIBITEUR D'ENZYME Ketoglutaric Acids - metabolism Kinetics Mixed Function Oxygenases - antagonists & inhibitors Molecular Sequence Data Oxidoreductases OXIDORREDUCTASAS OXYDOREDUCTASE Peptide Fragments - analysis Peptide Mapping REACCIONES QUIMICAS REACTION CHIMIQUE RHODOTORULA THYMINE TIMINA TIROSINA Trypsin TYROSINE Uracil - analogs & derivatives Uracil - pharmacology
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