Preparation of monolayer smears from paraffin‐embedded tissue for image cytometry
A method is described for the preparation of monolayer smears from paraffin‐embedded tissue suitable for automated image analysis and DNA measurements. The proposed technique uses enzyme treatment and syringing for cell dispersal. Slide preparation is performed by centrifugal cytology. After Feulgen...
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| Veröffentlicht in: | Cytometry (New York, N.Y.) N.Y.), 1985-05, Vol.6 (3), p.268-272 |
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| container_title | Cytometry (New York, N.Y.) |
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| creator | van Driel‐Kulker, A. M. J. Mesker, W. E. van Velzen, I. Tanke, H. J. Feichtinger, J. Ploem, J. S. |
| description | A method is described for the preparation of monolayer smears from paraffin‐embedded tissue suitable for automated image analysis and DNA measurements. The proposed technique uses enzyme treatment and syringing for cell dispersal. Slide preparation is performed by centrifugal cytology. After Feulgen staining the quality of the monolayer smears is sufficiently high to enable visual morphologic evaluation. Automated DNA measurements using the Leyden television analysis system (LEYTAS) show coefficients of variation (CV) of 4.5% for the diploid cell population of the suspended tissue. This is approximately the same as the CV in fresh material from the same tumor. Formalin fixed trout red blood cells are used as reference cells. By applying image cytometry to paraffin‐embedded tissue this method allows retrospective studies of, for instance, the significan of DNA content with regard to the behavior of a tumor. |
| doi_str_mv | 10.1002/cyto.990060314 |
| format | Article |
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M. J. ; Mesker, W. E. ; van Velzen, I. ; Tanke, H. J. ; Feichtinger, J. ; Ploem, J. S.</creator><creatorcontrib>van Driel‐Kulker, A. M. J. ; Mesker, W. E. ; van Velzen, I. ; Tanke, H. J. ; Feichtinger, J. ; Ploem, J. S.</creatorcontrib><description>A method is described for the preparation of monolayer smears from paraffin‐embedded tissue suitable for automated image analysis and DNA measurements. The proposed technique uses enzyme treatment and syringing for cell dispersal. Slide preparation is performed by centrifugal cytology. After Feulgen staining the quality of the monolayer smears is sufficiently high to enable visual morphologic evaluation. Automated DNA measurements using the Leyden television analysis system (LEYTAS) show coefficients of variation (CV) of 4.5% for the diploid cell population of the suspended tissue. This is approximately the same as the CV in fresh material from the same tumor. Formalin fixed trout red blood cells are used as reference cells. 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M. J.</creatorcontrib><creatorcontrib>Mesker, W. E.</creatorcontrib><creatorcontrib>van Velzen, I.</creatorcontrib><creatorcontrib>Tanke, H. J.</creatorcontrib><creatorcontrib>Feichtinger, J.</creatorcontrib><creatorcontrib>Ploem, J. S.</creatorcontrib><title>Preparation of monolayer smears from paraffin‐embedded tissue for image cytometry</title><title>Cytometry (New York, N.Y.)</title><addtitle>Cytometry</addtitle><description>A method is described for the preparation of monolayer smears from paraffin‐embedded tissue suitable for automated image analysis and DNA measurements. The proposed technique uses enzyme treatment and syringing for cell dispersal. Slide preparation is performed by centrifugal cytology. After Feulgen staining the quality of the monolayer smears is sufficiently high to enable visual morphologic evaluation. Automated DNA measurements using the Leyden television analysis system (LEYTAS) show coefficients of variation (CV) of 4.5% for the diploid cell population of the suspended tissue. This is approximately the same as the CV in fresh material from the same tumor. Formalin fixed trout red blood cells are used as reference cells. By applying image cytometry to paraffin‐embedded tissue this method allows retrospective studies of, for instance, the significan of DNA content with regard to the behavior of a tumor.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>centrifugal cytology</subject><subject>Cytological Techniques</subject><subject>Cytology</subject><subject>Diverse techniques</subject><subject>DNA measurements</subject><subject>DNA, Neoplasm - analysis</subject><subject>Erythrocytes - analysis</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>histology</subject><subject>Humans</subject><subject>image cytometry</subject><subject>Molecular and cellular biology</subject><subject>monolayer preparation</subject><subject>Neoplasms - analysis</subject><subject>Neoplasms - pathology</subject><subject>Paraffin</subject><subject>paraffin blocks</subject><subject>Reference Standards</subject><subject>Trout</subject><issn>0196-4763</issn><issn>1097-0320</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9Kw0AQhxdRaq1evQl7EG-ps93NbvYoxX9QqGA9eAqbZFYiSbbupkhuPoLP6JOY0FKPwsAc5puZjx8h5wymDGB2nXetm2oNIIEzcUDGDLSKgM_gkIyBaRkJJfkxOQnhHQC0FHxERjxJkjhWY_L85HFtvGlL11Bnae0aV5kOPQ01Gh-o9a6mA2Ft2fx8fWOdYVFgQdsyhA1S6zwta_OGdDCpsfXdKTmypgp4tusT8nJ3u5o_RIvl_eP8ZhHlArSIFAipVAGZhLy3sTJLDHI1YzZTQmipCg2Z5owLzRNu4sxyJnMlbF82LiSfkKvt3bV3HxsMbVqXIceqMg26TUiVZEwlXPfgdAvm3oXg0aZr3zv7LmWQDimmg3u6T7FfuNhd3mQ1Fnt8F1s_v9zNTchNZb1p8jLsMT2L45jxHtNb7LOssPvnaTp_XS3_FH4BSQKM1w</recordid><startdate>198505</startdate><enddate>198505</enddate><creator>van Driel‐Kulker, A. 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Psychology</topic><topic>histology</topic><topic>Humans</topic><topic>image cytometry</topic><topic>Molecular and cellular biology</topic><topic>monolayer preparation</topic><topic>Neoplasms - analysis</topic><topic>Neoplasms - pathology</topic><topic>Paraffin</topic><topic>paraffin blocks</topic><topic>Reference Standards</topic><topic>Trout</topic><toplevel>online_resources</toplevel><creatorcontrib>van Driel‐Kulker, A. M. J.</creatorcontrib><creatorcontrib>Mesker, W. E.</creatorcontrib><creatorcontrib>van Velzen, I.</creatorcontrib><creatorcontrib>Tanke, H. J.</creatorcontrib><creatorcontrib>Feichtinger, J.</creatorcontrib><creatorcontrib>Ploem, J. 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S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparation of monolayer smears from paraffin‐embedded tissue for image cytometry</atitle><jtitle>Cytometry (New York, N.Y.)</jtitle><addtitle>Cytometry</addtitle><date>1985-05</date><risdate>1985</risdate><volume>6</volume><issue>3</issue><spage>268</spage><epage>272</epage><pages>268-272</pages><issn>0196-4763</issn><eissn>1097-0320</eissn><coden>CYTODQ</coden><abstract>A method is described for the preparation of monolayer smears from paraffin‐embedded tissue suitable for automated image analysis and DNA measurements. The proposed technique uses enzyme treatment and syringing for cell dispersal. Slide preparation is performed by centrifugal cytology. After Feulgen staining the quality of the monolayer smears is sufficiently high to enable visual morphologic evaluation. Automated DNA measurements using the Leyden television analysis system (LEYTAS) show coefficients of variation (CV) of 4.5% for the diploid cell population of the suspended tissue. This is approximately the same as the CV in fresh material from the same tumor. Formalin fixed trout red blood cells are used as reference cells. By applying image cytometry to paraffin‐embedded tissue this method allows retrospective studies of, for instance, the significan of DNA content with regard to the behavior of a tumor.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>3888557</pmid><doi>10.1002/cyto.990060314</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0196-4763 |
| ispartof | Cytometry (New York, N.Y.), 1985-05, Vol.6 (3), p.268-272 |
| issn | 0196-4763 1097-0320 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Animals Biological and medical sciences centrifugal cytology Cytological Techniques Cytology Diverse techniques DNA measurements DNA, Neoplasm - analysis Erythrocytes - analysis Female Fundamental and applied biological sciences. Psychology histology Humans image cytometry Molecular and cellular biology monolayer preparation Neoplasms - analysis Neoplasms - pathology Paraffin paraffin blocks Reference Standards Trout |
| title | Preparation of monolayer smears from paraffin‐embedded tissue for image cytometry |
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