Development of a human granulosa cell culture model with follicle stimulating hormone responsiveness

In order to study the effects of follicle-stimulating hormone (FSH) on differentiation of granulosa cells, a well-defined and validated in-vitro culture system is indispensable. In this study, pooled follicular aspirates were stimulated in vitro with FSH and luteinizing hormone (LH) for 2, 4 and 6 d...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Human reproduction (Oxford) 1993-09, Vol.8 (9), p.1380-1386
Hauptverfasser: SCHIPPER, I, FAUSER, B. C. J. M, VAN GAVER, E. B. O, ZARUTSKIE, P. W, DAHL, K. D
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1386
container_issue 9
container_start_page 1380
container_title Human reproduction (Oxford)
container_volume 8
creator SCHIPPER, I
FAUSER, B. C. J. M
VAN GAVER, E. B. O
ZARUTSKIE, P. W
DAHL, K. D
description In order to study the effects of follicle-stimulating hormone (FSH) on differentiation of granulosa cells, a well-defined and validated in-vitro culture system is indispensable. In this study, pooled follicular aspirates were stimulated in vitro with FSH and luteinizing hormone (LH) for 2, 4 and 6 days, either immediately after plating or after 7 days of preincubation. Cultures were assayed for progesterone and oestradiol production. Fresh cells displayed very high basal progesterone production which could be stimulated with LH but not FSH. After preincubation, addition of LH and FSH resulted in dose-dependent increases of progesterone and oestradiol. When cultured on human fibronectin-coated wells, similar basal but higher progesterone concentrations after stimulation were observed. In comparison with serum-free media, addition of Serum-Plus resulted in higher basal and stimulated progesterone concentration, possibly due to the presence of serum factors. This study demonstrates firstly that after 7 days preincubation, cultures gained responsiveness to FSH but remained responsive to LH during 4 days of stimulation. This suggests a persisting differentiated cell population in vitro. Secondly, the use of human fibronectin extracellular matrix and serum promotes steroid production, either due to factors promoting cell growth and function or to availability of steroid precursors. Therefore one has to be cautious with interpretation of data obtained from this widely used culture system, employing highly differentiated cells obtained after ovarian stimulation for in-vitro fertilization for study of local regulation of granulosa cell function.
doi_str_mv 10.1093/oxfordjournals.humrep.a138265
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_76105737</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>76105737</sourcerecordid><originalsourceid>FETCH-LOGICAL-p167t-3b5d801b6b5f2a6a84613e10f4c9b6d64597ed47d343d3176d7fec7a934906d53</originalsourceid><addsrcrecordid>eNo9kM1OwzAQhC0EKqXwCEg-ALcUO47t5IjKr1SJC5wjJ163qRw72HGBtyeIitOONJ9mR4PQNSVLSip267-MD3rnU3DKxuU29QGGpaKszAU_QnNaCJLljJNjNCe5KDNKBT1FZzHuCJlkKWZoVuacVXk-R_oe9mD90IMbsTdY4SlQObwJyiXro8ItWIvbZMcUAPdeg8Wf3bjFxlvbtRZwHLs-WTV2boO3PvTeAQ4QB-9itwcHMZ6jEzN1hYvDXaD3x4e31XO2fn16Wd2ts4EKOWas4boktBENN7kSqiwEZUCJKdqqEVoUvJKgC6lZwTSjUmhpoJWqYkVFhOZsgW7-cofgPxLEse67-NtfOfAp1lJQwiWTE3h5AFPTg66H0PUqfNeHWSb_6uCr2CprpjHaLv5jrCR8esl-AMhsefY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76105737</pqid></control><display><type>article</type><title>Development of a human granulosa cell culture model with follicle stimulating hormone responsiveness</title><source>MEDLINE</source><source>Oxford University Press Archive</source><creator>SCHIPPER, I ; FAUSER, B. C. J. M ; VAN GAVER, E. B. O ; ZARUTSKIE, P. W ; DAHL, K. D</creator><creatorcontrib>SCHIPPER, I ; FAUSER, B. C. J. M ; VAN GAVER, E. B. O ; ZARUTSKIE, P. W ; DAHL, K. D</creatorcontrib><description>In order to study the effects of follicle-stimulating hormone (FSH) on differentiation of granulosa cells, a well-defined and validated in-vitro culture system is indispensable. In this study, pooled follicular aspirates were stimulated in vitro with FSH and luteinizing hormone (LH) for 2, 4 and 6 days, either immediately after plating or after 7 days of preincubation. Cultures were assayed for progesterone and oestradiol production. Fresh cells displayed very high basal progesterone production which could be stimulated with LH but not FSH. After preincubation, addition of LH and FSH resulted in dose-dependent increases of progesterone and oestradiol. When cultured on human fibronectin-coated wells, similar basal but higher progesterone concentrations after stimulation were observed. In comparison with serum-free media, addition of Serum-Plus resulted in higher basal and stimulated progesterone concentration, possibly due to the presence of serum factors. This study demonstrates firstly that after 7 days preincubation, cultures gained responsiveness to FSH but remained responsive to LH during 4 days of stimulation. This suggests a persisting differentiated cell population in vitro. Secondly, the use of human fibronectin extracellular matrix and serum promotes steroid production, either due to factors promoting cell growth and function or to availability of steroid precursors. Therefore one has to be cautious with interpretation of data obtained from this widely used culture system, employing highly differentiated cells obtained after ovarian stimulation for in-vitro fertilization for study of local regulation of granulosa cell function.</description><identifier>ISSN: 0268-1161</identifier><identifier>EISSN: 1460-2350</identifier><identifier>DOI: 10.1093/oxfordjournals.humrep.a138265</identifier><identifier>PMID: 8253922</identifier><identifier>CODEN: HUREEE</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Basal Metabolism ; Biological and medical sciences ; Blood Physiological Phenomena ; Cells, Cultured ; Culture Media ; Estradiol - biosynthesis ; Extracellular Matrix - physiology ; Female ; Fertilization in Vitro ; Follicle Stimulating Hormone - physiology ; Follicular Phase - physiology ; Fundamental and applied biological sciences. Psychology ; Granulosa Cells - physiology ; Hormone metabolism and regulation ; Humans ; Luteinizing Hormone - physiology ; Mammalian female genital system ; Progesterone - biosynthesis ; Reproducibility of Results ; Vertebrates: reproduction</subject><ispartof>Human reproduction (Oxford), 1993-09, Vol.8 (9), p.1380-1386</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=3805490$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8253922$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SCHIPPER, I</creatorcontrib><creatorcontrib>FAUSER, B. C. J. M</creatorcontrib><creatorcontrib>VAN GAVER, E. B. O</creatorcontrib><creatorcontrib>ZARUTSKIE, P. W</creatorcontrib><creatorcontrib>DAHL, K. D</creatorcontrib><title>Development of a human granulosa cell culture model with follicle stimulating hormone responsiveness</title><title>Human reproduction (Oxford)</title><addtitle>Hum Reprod</addtitle><description>In order to study the effects of follicle-stimulating hormone (FSH) on differentiation of granulosa cells, a well-defined and validated in-vitro culture system is indispensable. In this study, pooled follicular aspirates were stimulated in vitro with FSH and luteinizing hormone (LH) for 2, 4 and 6 days, either immediately after plating or after 7 days of preincubation. Cultures were assayed for progesterone and oestradiol production. Fresh cells displayed very high basal progesterone production which could be stimulated with LH but not FSH. After preincubation, addition of LH and FSH resulted in dose-dependent increases of progesterone and oestradiol. When cultured on human fibronectin-coated wells, similar basal but higher progesterone concentrations after stimulation were observed. In comparison with serum-free media, addition of Serum-Plus resulted in higher basal and stimulated progesterone concentration, possibly due to the presence of serum factors. This study demonstrates firstly that after 7 days preincubation, cultures gained responsiveness to FSH but remained responsive to LH during 4 days of stimulation. This suggests a persisting differentiated cell population in vitro. Secondly, the use of human fibronectin extracellular matrix and serum promotes steroid production, either due to factors promoting cell growth and function or to availability of steroid precursors. Therefore one has to be cautious with interpretation of data obtained from this widely used culture system, employing highly differentiated cells obtained after ovarian stimulation for in-vitro fertilization for study of local regulation of granulosa cell function.</description><subject>Basal Metabolism</subject><subject>Biological and medical sciences</subject><subject>Blood Physiological Phenomena</subject><subject>Cells, Cultured</subject><subject>Culture Media</subject><subject>Estradiol - biosynthesis</subject><subject>Extracellular Matrix - physiology</subject><subject>Female</subject><subject>Fertilization in Vitro</subject><subject>Follicle Stimulating Hormone - physiology</subject><subject>Follicular Phase - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Granulosa Cells - physiology</subject><subject>Hormone metabolism and regulation</subject><subject>Humans</subject><subject>Luteinizing Hormone - physiology</subject><subject>Mammalian female genital system</subject><subject>Progesterone - biosynthesis</subject><subject>Reproducibility of Results</subject><subject>Vertebrates: reproduction</subject><issn>0268-1161</issn><issn>1460-2350</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1OwzAQhC0EKqXwCEg-ALcUO47t5IjKr1SJC5wjJ163qRw72HGBtyeIitOONJ9mR4PQNSVLSip267-MD3rnU3DKxuU29QGGpaKszAU_QnNaCJLljJNjNCe5KDNKBT1FZzHuCJlkKWZoVuacVXk-R_oe9mD90IMbsTdY4SlQObwJyiXro8ItWIvbZMcUAPdeg8Wf3bjFxlvbtRZwHLs-WTV2boO3PvTeAQ4QB-9itwcHMZ6jEzN1hYvDXaD3x4e31XO2fn16Wd2ts4EKOWas4boktBENN7kSqiwEZUCJKdqqEVoUvJKgC6lZwTSjUmhpoJWqYkVFhOZsgW7-cofgPxLEse67-NtfOfAp1lJQwiWTE3h5AFPTg66H0PUqfNeHWSb_6uCr2CprpjHaLv5jrCR8esl-AMhsefY</recordid><startdate>19930901</startdate><enddate>19930901</enddate><creator>SCHIPPER, I</creator><creator>FAUSER, B. C. J. M</creator><creator>VAN GAVER, E. B. O</creator><creator>ZARUTSKIE, P. W</creator><creator>DAHL, K. D</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19930901</creationdate><title>Development of a human granulosa cell culture model with follicle stimulating hormone responsiveness</title><author>SCHIPPER, I ; FAUSER, B. C. J. M ; VAN GAVER, E. B. O ; ZARUTSKIE, P. W ; DAHL, K. D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p167t-3b5d801b6b5f2a6a84613e10f4c9b6d64597ed47d343d3176d7fec7a934906d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Basal Metabolism</topic><topic>Biological and medical sciences</topic><topic>Blood Physiological Phenomena</topic><topic>Cells, Cultured</topic><topic>Culture Media</topic><topic>Estradiol - biosynthesis</topic><topic>Extracellular Matrix - physiology</topic><topic>Female</topic><topic>Fertilization in Vitro</topic><topic>Follicle Stimulating Hormone - physiology</topic><topic>Follicular Phase - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Granulosa Cells - physiology</topic><topic>Hormone metabolism and regulation</topic><topic>Humans</topic><topic>Luteinizing Hormone - physiology</topic><topic>Mammalian female genital system</topic><topic>Progesterone - biosynthesis</topic><topic>Reproducibility of Results</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SCHIPPER, I</creatorcontrib><creatorcontrib>FAUSER, B. C. J. M</creatorcontrib><creatorcontrib>VAN GAVER, E. B. O</creatorcontrib><creatorcontrib>ZARUTSKIE, P. W</creatorcontrib><creatorcontrib>DAHL, K. D</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Human reproduction (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SCHIPPER, I</au><au>FAUSER, B. C. J. M</au><au>VAN GAVER, E. B. O</au><au>ZARUTSKIE, P. W</au><au>DAHL, K. D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a human granulosa cell culture model with follicle stimulating hormone responsiveness</atitle><jtitle>Human reproduction (Oxford)</jtitle><addtitle>Hum Reprod</addtitle><date>1993-09-01</date><risdate>1993</risdate><volume>8</volume><issue>9</issue><spage>1380</spage><epage>1386</epage><pages>1380-1386</pages><issn>0268-1161</issn><eissn>1460-2350</eissn><coden>HUREEE</coden><abstract>In order to study the effects of follicle-stimulating hormone (FSH) on differentiation of granulosa cells, a well-defined and validated in-vitro culture system is indispensable. In this study, pooled follicular aspirates were stimulated in vitro with FSH and luteinizing hormone (LH) for 2, 4 and 6 days, either immediately after plating or after 7 days of preincubation. Cultures were assayed for progesterone and oestradiol production. Fresh cells displayed very high basal progesterone production which could be stimulated with LH but not FSH. After preincubation, addition of LH and FSH resulted in dose-dependent increases of progesterone and oestradiol. When cultured on human fibronectin-coated wells, similar basal but higher progesterone concentrations after stimulation were observed. In comparison with serum-free media, addition of Serum-Plus resulted in higher basal and stimulated progesterone concentration, possibly due to the presence of serum factors. This study demonstrates firstly that after 7 days preincubation, cultures gained responsiveness to FSH but remained responsive to LH during 4 days of stimulation. This suggests a persisting differentiated cell population in vitro. Secondly, the use of human fibronectin extracellular matrix and serum promotes steroid production, either due to factors promoting cell growth and function or to availability of steroid precursors. Therefore one has to be cautious with interpretation of data obtained from this widely used culture system, employing highly differentiated cells obtained after ovarian stimulation for in-vitro fertilization for study of local regulation of granulosa cell function.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>8253922</pmid><doi>10.1093/oxfordjournals.humrep.a138265</doi><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0268-1161
ispartof Human reproduction (Oxford), 1993-09, Vol.8 (9), p.1380-1386
issn 0268-1161
1460-2350
language eng
recordid cdi_proquest_miscellaneous_76105737
source MEDLINE; Oxford University Press Archive
subjects Basal Metabolism
Biological and medical sciences
Blood Physiological Phenomena
Cells, Cultured
Culture Media
Estradiol - biosynthesis
Extracellular Matrix - physiology
Female
Fertilization in Vitro
Follicle Stimulating Hormone - physiology
Follicular Phase - physiology
Fundamental and applied biological sciences. Psychology
Granulosa Cells - physiology
Hormone metabolism and regulation
Humans
Luteinizing Hormone - physiology
Mammalian female genital system
Progesterone - biosynthesis
Reproducibility of Results
Vertebrates: reproduction
title Development of a human granulosa cell culture model with follicle stimulating hormone responsiveness
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T16%3A58%3A19IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20a%20human%20granulosa%20cell%20culture%20model%20with%20follicle%20stimulating%20hormone%20responsiveness&rft.jtitle=Human%20reproduction%20(Oxford)&rft.au=SCHIPPER,%20I&rft.date=1993-09-01&rft.volume=8&rft.issue=9&rft.spage=1380&rft.epage=1386&rft.pages=1380-1386&rft.issn=0268-1161&rft.eissn=1460-2350&rft.coden=HUREEE&rft_id=info:doi/10.1093/oxfordjournals.humrep.a138265&rft_dat=%3Cproquest_pubme%3E76105737%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76105737&rft_id=info:pmid/8253922&rfr_iscdi=true