A major surface antigen of procyclic stage Trypanosoma congolense

Five monoclonal antibodies (mAb) were raised that bound to the surface of procyclic stage Trypanosoma congolense with high intensity in immunofluorescence. Immunoblot analysis of trypanosome lysates using 3 of these mAb revealed a diffuse SDS-PAGE band of 36-40 kDa. The purified antigen did not reac...

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Veröffentlicht in:	Molecular and biochemical parasitology 1993-10, Vol.61 (2), p.295-310
Hauptverfasser:	Bayne, Rosemary A.L., Kilbride, Elizabeth A., Lainson, F.Alex, Tetley, Laurence, Barry, J.David
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Antibodies, Monoclonal Antigens, Protozoan - analysis Antigens, Protozoan - biosynthesis Antigens, Surface - analysis Antigens, Surface - biosynthesis Base Sequence Biochemistry. Physiology. Immunology. Molecular biology Biological and medical sciences Codon DNA Primers DNA, Protozoan - isolation & purification DNA, Protozoan - metabolism Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Genomic Library Glutamate and alanine-rich protein Immunoblotting Invertebrates Molecular Sequence Data Procyclin Protozoa Restriction Mapping RNA Splicing RNA, Messenger - isolation & purification RNA, Messenger - metabolism RNA, Protozoan - isolation & purification RNA, Protozoan - metabolism Surface glycoprotein Trypanosoma congolense Trypanosoma congolense - immunology Trypanosoma congolense - physiology
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