Effects of polyphyllin I on growth inhibition of human non-small lung cancer cells and in xenograft
Polyphyllin I （PPI）, a small molecular monomer extracted from Rhizoma of Paris polyphyllin, shows strong antieancer effects in previous study. Human lung adenocarcinoma A549 cells, human lung squamous cell carcinoma SK-MES-1 cells, and human lung large cell carcinoma H460 cells were cultured and the...
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| Veröffentlicht in: | Acta biochimica et biophysica Sinica 2010-11, Vol.42 (11), p.827-833 |
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| description | Polyphyllin I （PPI）, a small molecular monomer extracted from Rhizoma of Paris polyphyllin, shows strong antieancer effects in previous study. Human lung adenocarcinoma A549 cells, human lung squamous cell carcinoma SK-MES-1 cells, and human lung large cell carcinoma H460 cells were cultured and then treated with PPI. Cell proliferation and apoptosis were measured by 3-[4,5- dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, flow cytometry, western blot analysis, and DNA ladder. Athymic nude mice bearing tumors were injected with PPI, and tumor growth was recorded. Our results showed that PPI significantly inhibited the proliferation of three non-small cell lung cancer （NSCLC） cell lines, with the inhibitory concentrations （IC50） of 1.24, 2.40, and 2.33 μg/ml for A549, H460, and SK-MES-1 cells, respectively. After being treated with 2.5 μg/ml of PPI for 24 h, the apoptotic rate of A549 cells was 39.68%, which was remarkably higher than that of the control. Tumor growth was significantly inhibited in the PPI-treated group compared with the group treated with cisplatin （DDP） or PBS in the nude mice. PPI exhibits antitumor ability in NSCLC cells in vitro and in vivo, which might be related to the apoptosis induced by PPI. |
| doi_str_mv | 10.1093/abbs/gmq091 |
| format | Article |
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Human lung adenocarcinoma A549 cells, human lung squamous cell carcinoma SK-MES-1 cells, and human lung large cell carcinoma H460 cells were cultured and then treated with PPI. Cell proliferation and apoptosis were measured by 3-[4,5- dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, flow cytometry, western blot analysis, and DNA ladder. Athymic nude mice bearing tumors were injected with PPI, and tumor growth was recorded. Our results showed that PPI significantly inhibited the proliferation of three non-small cell lung cancer （NSCLC） cell lines, with the inhibitory concentrations （IC50） of 1.24, 2.40, and 2.33 μg/ml for A549, H460, and SK-MES-1 cells, respectively. After being treated with 2.5 μg/ml of PPI for 24 h, the apoptotic rate of A549 cells was 39.68%, which was remarkably higher than that of the control. Tumor growth was significantly inhibited in the PPI-treated group compared with the group treated with cisplatin （DDP） or PBS in the nude mice. PPI exhibits antitumor ability in NSCLC cells in vitro and in vivo, which might be related to the apoptosis induced by PPI.</description><identifier>ISSN: 1672-9145</identifier><identifier>EISSN: 1745-7270</identifier><identifier>DOI: 10.1093/abbs/gmq091</identifier><identifier>PMID: 20978038</identifier><language>eng</language><publisher>China</publisher><subject>A549细胞 ; Animals ; Apoptosis - drug effects ; Blotting, Western ; Carcinoma, Non-Small-Cell Lung - drug therapy ; Carcinoma, Non-Small-Cell Lung - metabolism ; Carcinoma, Non-Small-Cell Lung - pathology ; Caspase 3 - metabolism ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Diosgenin - analogs & derivatives ; Diosgenin - chemistry ; Diosgenin - pharmacology ; Dose-Response Relationship, Drug ; Down-Regulation - drug effects ; Humans ; Inhibitory Concentration 50 ; Lung Neoplasms - drug therapy ; Lung Neoplasms - metabolism ; Lung Neoplasms - pathology ; Male ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Molecular Structure ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Rhizome - chemistry ; Time Factors ; Tumor Burden - drug effects ; Xenograft Model Antitumor Assays ; 价格指数 ; 抑制作用 ; 经济增长 ; 肺癌细胞 ; 肿瘤生长</subject><ispartof>Acta biochimica et biophysica Sinica, 2010-11, Vol.42 (11), p.827-833</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-f699a728075932ea47b5468d76aeb82c171883f449f6f52f635c31e388733b93</citedby><cites>FETCH-LOGICAL-c418t-f699a728075932ea47b5468d76aeb82c171883f449f6f52f635c31e388733b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/90160X/90160X.jpg</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20978038$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kong, Minjian</creatorcontrib><creatorcontrib>Fan, Junqiang</creatorcontrib><creatorcontrib>Dong, Aiqiang</creatorcontrib><creatorcontrib>Cheng, Haifeng</creatorcontrib><creatorcontrib>Xu, Rongzhen</creatorcontrib><title>Effects of polyphyllin I on growth inhibition of human non-small lung cancer cells and in xenograft</title><title>Acta biochimica et biophysica Sinica</title><addtitle>Acta Biochimica et Biophysica Sinica</addtitle><description>Polyphyllin I （PPI）, a small molecular monomer extracted from Rhizoma of Paris polyphyllin, shows strong antieancer effects in previous study. Human lung adenocarcinoma A549 cells, human lung squamous cell carcinoma SK-MES-1 cells, and human lung large cell carcinoma H460 cells were cultured and then treated with PPI. Cell proliferation and apoptosis were measured by 3-[4,5- dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, flow cytometry, western blot analysis, and DNA ladder. Athymic nude mice bearing tumors were injected with PPI, and tumor growth was recorded. Our results showed that PPI significantly inhibited the proliferation of three non-small cell lung cancer （NSCLC） cell lines, with the inhibitory concentrations （IC50） of 1.24, 2.40, and 2.33 μg/ml for A549, H460, and SK-MES-1 cells, respectively. After being treated with 2.5 μg/ml of PPI for 24 h, the apoptotic rate of A549 cells was 39.68%, which was remarkably higher than that of the control. Tumor growth was significantly inhibited in the PPI-treated group compared with the group treated with cisplatin （DDP） or PBS in the nude mice. PPI exhibits antitumor ability in NSCLC cells in vitro and in vivo, which might be related to the apoptosis induced by PPI.</description><subject>A549细胞</subject><subject>Animals</subject><subject>Apoptosis - drug effects</subject><subject>Blotting, Western</subject><subject>Carcinoma, Non-Small-Cell Lung - drug therapy</subject><subject>Carcinoma, Non-Small-Cell Lung - metabolism</subject><subject>Carcinoma, Non-Small-Cell Lung - pathology</subject><subject>Caspase 3 - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Diosgenin - analogs & derivatives</subject><subject>Diosgenin - chemistry</subject><subject>Diosgenin - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>Down-Regulation - drug effects</subject><subject>Humans</subject><subject>Inhibitory Concentration 50</subject><subject>Lung Neoplasms - drug therapy</subject><subject>Lung Neoplasms - metabolism</subject><subject>Lung Neoplasms - pathology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>Molecular Structure</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Rhizome - chemistry</subject><subject>Time Factors</subject><subject>Tumor Burden - drug effects</subject><subject>Xenograft Model Antitumor Assays</subject><subject>价格指数</subject><subject>抑制作用</subject><subject>经济增长</subject><subject>肺癌细胞</subject><subject>肿瘤生长</subject><issn>1672-9145</issn><issn>1745-7270</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kElPwzAQRi0EYimcuCOLCwcU8JJ4OaKqLBISl94tx7UTg2O3cSLovydVC6cZjd58mnkAXGP0gJGkj7qu82PTbZDER-Ac87IqOOHoeOoZJ4XEZXUGLnL-RIgyhtEpOCNIcoGoOAdm4Zw1Q4bJwXUK23W7DcFH-AZThE2fvocW-tj62g9-mkxUO3Y6wphikTsdAgxjbKDR0dgeGhtChjquph34Y2Nqeu2GS3DidMj26lBnYPm8WM5fi_ePl7f503thSiyGwjEpNScC8UpSYnXJ66pkYsWZtrUgBnMsBHVlKR1zFXGMVoZiS4XglNaSzsDdPnbdp81o86A6n3cX6WjTmBWffi-JkGwi7_ek6VPOvXVq3ftO91uFkdo5VTunau90om8OuWPd2dU_-ydxAm4PcW2KzcbHRtXafDkfrKKV5IRWmP4CXXh-gQ</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Kong, Minjian</creator><creator>Fan, Junqiang</creator><creator>Dong, Aiqiang</creator><creator>Cheng, Haifeng</creator><creator>Xu, Rongzhen</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>WU4</scope><scope>~WA</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101101</creationdate><title>Effects of polyphyllin I on growth inhibition of human non-small lung cancer cells and in xenograft</title><author>Kong, Minjian ; Fan, Junqiang ; Dong, Aiqiang ; Cheng, Haifeng ; Xu, Rongzhen</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-f699a728075932ea47b5468d76aeb82c171883f449f6f52f635c31e388733b93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>A549细胞</topic><topic>Animals</topic><topic>Apoptosis - drug effects</topic><topic>Blotting, Western</topic><topic>Carcinoma, Non-Small-Cell Lung - drug therapy</topic><topic>Carcinoma, Non-Small-Cell Lung - metabolism</topic><topic>Carcinoma, Non-Small-Cell Lung - pathology</topic><topic>Caspase 3 - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Diosgenin - analogs & derivatives</topic><topic>Diosgenin - chemistry</topic><topic>Diosgenin - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Down-Regulation - drug effects</topic><topic>Humans</topic><topic>Inhibitory Concentration 50</topic><topic>Lung Neoplasms - drug therapy</topic><topic>Lung Neoplasms - metabolism</topic><topic>Lung Neoplasms - pathology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>Molecular Structure</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Rhizome - chemistry</topic><topic>Time Factors</topic><topic>Tumor Burden - drug effects</topic><topic>Xenograft Model Antitumor Assays</topic><topic>价格指数</topic><topic>抑制作用</topic><topic>经济增长</topic><topic>肺癌细胞</topic><topic>肿瘤生长</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kong, Minjian</creatorcontrib><creatorcontrib>Fan, Junqiang</creatorcontrib><creatorcontrib>Dong, Aiqiang</creatorcontrib><creatorcontrib>Cheng, Haifeng</creatorcontrib><creatorcontrib>Xu, Rongzhen</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库-自然科学-生物科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta biochimica et biophysica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kong, Minjian</au><au>Fan, Junqiang</au><au>Dong, Aiqiang</au><au>Cheng, Haifeng</au><au>Xu, Rongzhen</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of polyphyllin I on growth inhibition of human non-small lung cancer cells and in xenograft</atitle><jtitle>Acta biochimica et biophysica Sinica</jtitle><addtitle>Acta Biochimica et Biophysica Sinica</addtitle><date>2010-11-01</date><risdate>2010</risdate><volume>42</volume><issue>11</issue><spage>827</spage><epage>833</epage><pages>827-833</pages><issn>1672-9145</issn><eissn>1745-7270</eissn><abstract>Polyphyllin I （PPI）, a small molecular monomer extracted from Rhizoma of Paris polyphyllin, shows strong antieancer effects in previous study. Human lung adenocarcinoma A549 cells, human lung squamous cell carcinoma SK-MES-1 cells, and human lung large cell carcinoma H460 cells were cultured and then treated with PPI. Cell proliferation and apoptosis were measured by 3-[4,5- dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, flow cytometry, western blot analysis, and DNA ladder. Athymic nude mice bearing tumors were injected with PPI, and tumor growth was recorded. Our results showed that PPI significantly inhibited the proliferation of three non-small cell lung cancer （NSCLC） cell lines, with the inhibitory concentrations （IC50） of 1.24, 2.40, and 2.33 μg/ml for A549, H460, and SK-MES-1 cells, respectively. After being treated with 2.5 μg/ml of PPI for 24 h, the apoptotic rate of A549 cells was 39.68%, which was remarkably higher than that of the control. Tumor growth was significantly inhibited in the PPI-treated group compared with the group treated with cisplatin （DDP） or PBS in the nude mice. PPI exhibits antitumor ability in NSCLC cells in vitro and in vivo, which might be related to the apoptosis induced by PPI.</abstract><cop>China</cop><pmid>20978038</pmid><doi>10.1093/abbs/gmq091</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | A549细胞 Animals Apoptosis - drug effects Blotting, Western Carcinoma, Non-Small-Cell Lung - drug therapy Carcinoma, Non-Small-Cell Lung - metabolism Carcinoma, Non-Small-Cell Lung - pathology Caspase 3 - metabolism Cell Line, Tumor Cell Proliferation - drug effects Diosgenin - analogs & derivatives Diosgenin - chemistry Diosgenin - pharmacology Dose-Response Relationship, Drug Down-Regulation - drug effects Humans Inhibitory Concentration 50 Lung Neoplasms - drug therapy Lung Neoplasms - metabolism Lung Neoplasms - pathology Male Mice Mice, Inbred BALB C Mice, Nude Molecular Structure Plant Extracts - chemistry Plant Extracts - pharmacology Proto-Oncogene Proteins c-bcl-2 - metabolism Rhizome - chemistry Time Factors Tumor Burden - drug effects Xenograft Model Antitumor Assays 价格指数 抑制作用 经济增长 肺癌细胞 肿瘤生长 |
| title | Effects of polyphyllin I on growth inhibition of human non-small lung cancer cells and in xenograft |
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