Induction of immediate-early genes by angiotensin II and endothelin-1 in adult rat cardiomyocytes
OBJECTIVE:Few molecular signals for induction of myocardial hypertrophy have been identified. This study was carried out to investigate the action of angiotensin II and endothelin on the growth- and differentiation-related genes Egr-1 (early growth response gene 1) and c-fos in isolated adult rat ca...
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| Veröffentlicht in: | Journal of hypertension 1993-09, Vol.11 (9), p.927-934 |
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| container_title | Journal of hypertension |
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| creator | Neyses, Ludwig Nouskas, Janis Luyken, Joachim Fronhoffs, Stefan Oberdorf, Silke Pfeifer, Ulrich Williams, R Sanders Sukhatme, Vikas P Vetter, Hans |
| description | OBJECTIVE:Few molecular signals for induction of myocardial hypertrophy have been identified. This study was carried out to investigate the action of angiotensin II and endothelin on the growth- and differentiation-related genes Egr-1 (early growth response gene 1) and c-fos in isolated adult rat cardiomyocytes.
METHODS:Cardiac myocytes from male Wistar-Kyoto rats were isolated and incubated with angiotensin II and endothelin-1 in Dulbeccoʼs modified Eagleʼs medium. RNA was isolated and blotted, and densitometric analysis was performed. All experiments were repeated at least three times.
RESULTSEndothelin-1 (10mmol/l) induced a 20-25-fold rise in Egr-1 messenger RNA within 15min. This effect was dose-dependent, c-fos was induced 10-20-fold within 15 min with similar dose-response characteristics. Angiotensin II also induced Egr-1 and c-fos with kinetics similar to endothelin but a cofactor from fetal calf serum was needed for full c-fos expression. The protein kinase C activator phorbol 12-myristate 13-acetate also induced Egr-1.
CONCLUSIONS:The results identify Egr-1 and c-fos as target genes for the action of endothelin and angiotensin II in the adult myocardium suggesting that induction of the genes may be part of the signal transduction pathway for angiotensin II and endothelin in the myocardium. |
| doi_str_mv | 10.1097/00004872-199309000-00006 |
| format | Article |
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METHODS:Cardiac myocytes from male Wistar-Kyoto rats were isolated and incubated with angiotensin II and endothelin-1 in Dulbeccoʼs modified Eagleʼs medium. RNA was isolated and blotted, and densitometric analysis was performed. All experiments were repeated at least three times.
RESULTSEndothelin-1 (10mmol/l) induced a 20-25-fold rise in Egr-1 messenger RNA within 15min. This effect was dose-dependent, c-fos was induced 10-20-fold within 15 min with similar dose-response characteristics. Angiotensin II also induced Egr-1 and c-fos with kinetics similar to endothelin but a cofactor from fetal calf serum was needed for full c-fos expression. The protein kinase C activator phorbol 12-myristate 13-acetate also induced Egr-1.
CONCLUSIONS:The results identify Egr-1 and c-fos as target genes for the action of endothelin and angiotensin II in the adult myocardium suggesting that induction of the genes may be part of the signal transduction pathway for angiotensin II and endothelin in the myocardium.</description><identifier>ISSN: 0263-6352</identifier><identifier>EISSN: 1473-5598</identifier><identifier>DOI: 10.1097/00004872-199309000-00006</identifier><identifier>PMID: 7504706</identifier><identifier>CODEN: JOHYD3</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott-Raven Publishers</publisher><subject>Angiotensin II - pharmacology ; Animals ; Biological and medical sciences ; Cardiomegaly - etiology ; Cells, Cultured ; DNA-Binding Proteins - genetics ; Early Growth Response Protein 1 ; Endocrine kidney. Renin-angiotensin-aldosterone system ; Endothelins - pharmacology ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation - drug effects ; Genes, fos - drug effects ; Genes, Immediate-Early - drug effects ; Immediate-Early Proteins ; Male ; Myocardium - cytology ; Myocardium - metabolism ; Rats ; Rats, Inbred WKY ; RNA - analysis ; Transcription Factors - genetics ; Vertebrates: endocrinology</subject><ispartof>Journal of hypertension, 1993-09, Vol.11 (9), p.927-934</ispartof><rights>Lippincott-Raven Publishers.</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3656-de41a48a312e57739ba07d1d86c3992b0fc0b19d7471ca158032927e296cc5633</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4891173$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7504706$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Neyses, Ludwig</creatorcontrib><creatorcontrib>Nouskas, Janis</creatorcontrib><creatorcontrib>Luyken, Joachim</creatorcontrib><creatorcontrib>Fronhoffs, Stefan</creatorcontrib><creatorcontrib>Oberdorf, Silke</creatorcontrib><creatorcontrib>Pfeifer, Ulrich</creatorcontrib><creatorcontrib>Williams, R Sanders</creatorcontrib><creatorcontrib>Sukhatme, Vikas P</creatorcontrib><creatorcontrib>Vetter, Hans</creatorcontrib><title>Induction of immediate-early genes by angiotensin II and endothelin-1 in adult rat cardiomyocytes</title><title>Journal of hypertension</title><addtitle>J Hypertens</addtitle><description>OBJECTIVE:Few molecular signals for induction of myocardial hypertrophy have been identified. This study was carried out to investigate the action of angiotensin II and endothelin on the growth- and differentiation-related genes Egr-1 (early growth response gene 1) and c-fos in isolated adult rat cardiomyocytes.
METHODS:Cardiac myocytes from male Wistar-Kyoto rats were isolated and incubated with angiotensin II and endothelin-1 in Dulbeccoʼs modified Eagleʼs medium. RNA was isolated and blotted, and densitometric analysis was performed. All experiments were repeated at least three times.
RESULTSEndothelin-1 (10mmol/l) induced a 20-25-fold rise in Egr-1 messenger RNA within 15min. This effect was dose-dependent, c-fos was induced 10-20-fold within 15 min with similar dose-response characteristics. Angiotensin II also induced Egr-1 and c-fos with kinetics similar to endothelin but a cofactor from fetal calf serum was needed for full c-fos expression. The protein kinase C activator phorbol 12-myristate 13-acetate also induced Egr-1.
CONCLUSIONS:The results identify Egr-1 and c-fos as target genes for the action of endothelin and angiotensin II in the adult myocardium suggesting that induction of the genes may be part of the signal transduction pathway for angiotensin II and endothelin in the myocardium.</description><subject>Angiotensin II - pharmacology</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cardiomegaly - etiology</subject><subject>Cells, Cultured</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Early Growth Response Protein 1</subject><subject>Endocrine kidney. Renin-angiotensin-aldosterone system</subject><subject>Endothelins - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Genes, fos - drug effects</subject><subject>Genes, Immediate-Early - drug effects</subject><subject>Immediate-Early Proteins</subject><subject>Male</subject><subject>Myocardium - cytology</subject><subject>Myocardium - metabolism</subject><subject>Rats</subject><subject>Rats, Inbred WKY</subject><subject>RNA - analysis</subject><subject>Transcription Factors - genetics</subject><subject>Vertebrates: endocrinology</subject><issn>0263-6352</issn><issn>1473-5598</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUGP1CAUx4nRrLOrH8GEg_HWFUoL5Wg2rk6yiRc9k1d43UEprECz6be344xzkwt5__d7QH4QQjm75Uyrj2xb3aDahmstmN6q5hjJF2THOyWavtfDS7JjrRSNFH37mlyX8nMjBq3EFblSPesUkzsC--gWW32KNE3UzzM6DxUbhBxW-ogRCx1XCvHRp4qx-Ej3-610FKNL9YDBx4bTLQa3hEozVGohO5_mNdm1YnlDXk0QCr497zfkx_3n73dfm4dvX_Z3nx4aK2QvG4cdh24AwVvslRJ6BKYcd4O0Qut2ZJNlI9dOdYpb4P3ARKtbha2W1vZSiBvy4XTuU06_FyzVzL5YDAEipqUYJTkTUskNHE6gzamUjJN5yn6GvBrOzNGu-WfXXOz-jY6j7853LONm6jJ41rn135_7UCyEKUO0vlywbtCcq-NTuxP2nELFXH6F5RmzOSCEejD_-1vxB9wIkMo</recordid><startdate>199309</startdate><enddate>199309</enddate><creator>Neyses, Ludwig</creator><creator>Nouskas, Janis</creator><creator>Luyken, Joachim</creator><creator>Fronhoffs, Stefan</creator><creator>Oberdorf, Silke</creator><creator>Pfeifer, Ulrich</creator><creator>Williams, R Sanders</creator><creator>Sukhatme, Vikas P</creator><creator>Vetter, Hans</creator><general>Lippincott-Raven Publishers</general><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199309</creationdate><title>Induction of immediate-early genes by angiotensin II and endothelin-1 in adult rat cardiomyocytes</title><author>Neyses, Ludwig ; Nouskas, Janis ; Luyken, Joachim ; Fronhoffs, Stefan ; Oberdorf, Silke ; Pfeifer, Ulrich ; Williams, R Sanders ; Sukhatme, Vikas P ; Vetter, Hans</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3656-de41a48a312e57739ba07d1d86c3992b0fc0b19d7471ca158032927e296cc5633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Angiotensin II - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cardiomegaly - etiology</topic><topic>Cells, Cultured</topic><topic>DNA-Binding Proteins - genetics</topic><topic>Early Growth Response Protein 1</topic><topic>Endocrine kidney. Renin-angiotensin-aldosterone system</topic><topic>Endothelins - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Genes, fos - drug effects</topic><topic>Genes, Immediate-Early - drug effects</topic><topic>Immediate-Early Proteins</topic><topic>Male</topic><topic>Myocardium - cytology</topic><topic>Myocardium - metabolism</topic><topic>Rats</topic><topic>Rats, Inbred WKY</topic><topic>RNA - analysis</topic><topic>Transcription Factors - genetics</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Neyses, Ludwig</creatorcontrib><creatorcontrib>Nouskas, Janis</creatorcontrib><creatorcontrib>Luyken, Joachim</creatorcontrib><creatorcontrib>Fronhoffs, Stefan</creatorcontrib><creatorcontrib>Oberdorf, Silke</creatorcontrib><creatorcontrib>Pfeifer, Ulrich</creatorcontrib><creatorcontrib>Williams, R Sanders</creatorcontrib><creatorcontrib>Sukhatme, Vikas P</creatorcontrib><creatorcontrib>Vetter, Hans</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of hypertension</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Neyses, Ludwig</au><au>Nouskas, Janis</au><au>Luyken, Joachim</au><au>Fronhoffs, Stefan</au><au>Oberdorf, Silke</au><au>Pfeifer, Ulrich</au><au>Williams, R Sanders</au><au>Sukhatme, Vikas P</au><au>Vetter, Hans</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Induction of immediate-early genes by angiotensin II and endothelin-1 in adult rat cardiomyocytes</atitle><jtitle>Journal of hypertension</jtitle><addtitle>J Hypertens</addtitle><date>1993-09</date><risdate>1993</risdate><volume>11</volume><issue>9</issue><spage>927</spage><epage>934</epage><pages>927-934</pages><issn>0263-6352</issn><eissn>1473-5598</eissn><coden>JOHYD3</coden><abstract>OBJECTIVE:Few molecular signals for induction of myocardial hypertrophy have been identified. This study was carried out to investigate the action of angiotensin II and endothelin on the growth- and differentiation-related genes Egr-1 (early growth response gene 1) and c-fos in isolated adult rat cardiomyocytes.
METHODS:Cardiac myocytes from male Wistar-Kyoto rats were isolated and incubated with angiotensin II and endothelin-1 in Dulbeccoʼs modified Eagleʼs medium. RNA was isolated and blotted, and densitometric analysis was performed. All experiments were repeated at least three times.
RESULTSEndothelin-1 (10mmol/l) induced a 20-25-fold rise in Egr-1 messenger RNA within 15min. This effect was dose-dependent, c-fos was induced 10-20-fold within 15 min with similar dose-response characteristics. Angiotensin II also induced Egr-1 and c-fos with kinetics similar to endothelin but a cofactor from fetal calf serum was needed for full c-fos expression. The protein kinase C activator phorbol 12-myristate 13-acetate also induced Egr-1.
CONCLUSIONS:The results identify Egr-1 and c-fos as target genes for the action of endothelin and angiotensin II in the adult myocardium suggesting that induction of the genes may be part of the signal transduction pathway for angiotensin II and endothelin in the myocardium.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott-Raven Publishers</pub><pmid>7504706</pmid><doi>10.1097/00004872-199309000-00006</doi><tpages>8</tpages></addata></record> |
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| subjects | Angiotensin II - pharmacology Animals Biological and medical sciences Cardiomegaly - etiology Cells, Cultured DNA-Binding Proteins - genetics Early Growth Response Protein 1 Endocrine kidney. Renin-angiotensin-aldosterone system Endothelins - pharmacology Fundamental and applied biological sciences. Psychology Gene Expression Regulation - drug effects Genes, fos - drug effects Genes, Immediate-Early - drug effects Immediate-Early Proteins Male Myocardium - cytology Myocardium - metabolism Rats Rats, Inbred WKY RNA - analysis Transcription Factors - genetics Vertebrates: endocrinology |
| title | Induction of immediate-early genes by angiotensin II and endothelin-1 in adult rat cardiomyocytes |
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