Increased viability of endometrial cells by in vitro treatment with di-(2-ethylhexyl) phthalate
Based on the findings of several reports that have shown an increased plasma level of di-(2-ethylhexyl) phthalate (DEHP) in women with endometriosis, the present study was designed to evaluate whether in vitro treatment with DEHP can increase viability of endometrial cells. Utilizing 3-(4,5-dimethyl...
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| Veröffentlicht in: | Fertility and sterility 2010-11, Vol.94 (6), p.2413-2416 |
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| container_title | Fertility and sterility |
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| creator | Kim, Young-Hoon, M.D., Ph.D Kim, Sung Hoon, M.D., Ph.D Lee, Hoi Woul, M.S Chae, Hee Dong, M.D., Ph.D Kim, Chung-Hoon, M.D., Ph.D Kang, Byung Moon, M.D., Ph.D |
| description | Based on the findings of several reports that have shown an increased plasma level of di-(2-ethylhexyl) phthalate (DEHP) in women with endometriosis, the present study was designed to evaluate whether in vitro treatment with DEHP can increase viability of endometrial cells. Utilizing 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide assay, fluorescent activated cell sorter analysis, and microscopic evaluation after Hoechst staining, we revealed that in vitro treatment with DEHP leads to increased viability of Ishikawa cells as well as endometrial stromal cells in serum-free condition and following exposure to hydrogen peroxide, which suggests that exposure to phthalate might play a role in the establishment of endometriosis. |
| doi_str_mv | 10.1016/j.fertnstert.2010.04.027 |
| format | Article |
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Utilizing 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide assay, fluorescent activated cell sorter analysis, and microscopic evaluation after Hoechst staining, we revealed that in vitro treatment with DEHP leads to increased viability of Ishikawa cells as well as endometrial stromal cells in serum-free condition and following exposure to hydrogen peroxide, which suggests that exposure to phthalate might play a role in the establishment of endometriosis.</description><identifier>ISSN: 0015-0282</identifier><identifier>EISSN: 1556-5653</identifier><identifier>DOI: 10.1016/j.fertnstert.2010.04.027</identifier><identifier>PMID: 20493477</identifier><identifier>CODEN: FESTAS</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Biological and medical sciences ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Diethylhexyl Phthalate - pharmacology ; Dose-Response Relationship, Drug ; Drug Evaluation, Preclinical ; endocrine disruptor ; endometriosis ; endometrium ; Endometrium - drug effects ; Endometrium - physiology ; Female ; Female genital diseases ; Gynecology. Andrology. Obstetrics ; Humans ; Internal Medicine ; Medical sciences ; Non tumoral diseases ; Obstetrics and Gynecology ; pathogenesis ; Phthalate ; Plasticizers - pharmacology ; Stromal Cells - drug effects ; Stromal Cells - physiology ; Time Factors ; Up-Regulation - drug effects</subject><ispartof>Fertility and sterility, 2010-11, Vol.94 (6), p.2413-2416</ispartof><rights>American Society for Reproductive Medicine</rights><rights>2010 American Society for Reproductive Medicine</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. 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Andrology. Obstetrics</subject><subject>Humans</subject><subject>Internal Medicine</subject><subject>Medical sciences</subject><subject>Non tumoral diseases</subject><subject>Obstetrics and Gynecology</subject><subject>pathogenesis</subject><subject>Phthalate</subject><subject>Plasticizers - pharmacology</subject><subject>Stromal Cells - drug effects</subject><subject>Stromal Cells - physiology</subject><subject>Time Factors</subject><subject>Up-Regulation - drug effects</subject><issn>0015-0282</issn><issn>1556-5653</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk2LFDEQhoMo7rj6FyQXUQ89VtJJuvsirIsfCwse3HtIJ9V0xp70mGRW-9-bZkYXPHlJQXjequKhCKEMtgyYerfbDhhzSLm8Ww7lG8QWePOIbJiUqpJK1o_JBoDJCnjLL8izlHYAoFjDn5ILDqKrRdNsiL4JNqJJ6Oi9N72ffF7oPFAMbt5jjt5M1OI0Jdov1IcC5TjTXCJ5jyHTnz6P1PnqDa8wj8s04q9leksPYx7NZDI-J08GMyV8ca6X5O7Tx7vrL9Xt188311e3lZXQ5kpKIxvesVoNbcs613ctcM6sclKgbQFQgFBW9rZvRc8bN7QK6w64Yk4gqy_J61PbQ5x_HDFlvfdp3dsEnI9JN4pBzVtZF7I9kTbOKUUc9CH6vYmLZqBXuXqnH-TqVa4GoYvcEn15HnLs9-j-Bv_YLMCrM2CSNdMQTbA-PXC1YJ1SonAfThwWI_ceo07WY7DofESbtZv9_2zz_p8mdvLBl7nfccG0m48xFOOa6cQ16G_rMay3wNYzEEXFbwtWsho</recordid><startdate>20101101</startdate><enddate>20101101</enddate><creator>Kim, Young-Hoon, M.D., Ph.D</creator><creator>Kim, Sung Hoon, M.D., Ph.D</creator><creator>Lee, Hoi Woul, M.S</creator><creator>Chae, Hee Dong, M.D., Ph.D</creator><creator>Kim, Chung-Hoon, M.D., Ph.D</creator><creator>Kang, Byung Moon, M.D., Ph.D</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101101</creationdate><title>Increased viability of endometrial cells by in vitro treatment with di-(2-ethylhexyl) phthalate</title><author>Kim, Young-Hoon, M.D., Ph.D ; Kim, Sung Hoon, M.D., Ph.D ; Lee, Hoi Woul, M.S ; Chae, Hee Dong, M.D., Ph.D ; Kim, Chung-Hoon, M.D., Ph.D ; Kang, Byung Moon, M.D., Ph.D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c508t-55a5729136f8819db980221c6d54ec800e4046c5bcb84b27df86e390261d4e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological and medical sciences</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Diethylhexyl Phthalate - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>Drug Evaluation, Preclinical</topic><topic>endocrine disruptor</topic><topic>endometriosis</topic><topic>endometrium</topic><topic>Endometrium - drug effects</topic><topic>Endometrium - physiology</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Gynecology. 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| subjects | Biological and medical sciences Cell Proliferation - drug effects Cell Survival - drug effects Cells, Cultured Diethylhexyl Phthalate - pharmacology Dose-Response Relationship, Drug Drug Evaluation, Preclinical endocrine disruptor endometriosis endometrium Endometrium - drug effects Endometrium - physiology Female Female genital diseases Gynecology. Andrology. Obstetrics Humans Internal Medicine Medical sciences Non tumoral diseases Obstetrics and Gynecology pathogenesis Phthalate Plasticizers - pharmacology Stromal Cells - drug effects Stromal Cells - physiology Time Factors Up-Regulation - drug effects |
| title | Increased viability of endometrial cells by in vitro treatment with di-(2-ethylhexyl) phthalate |
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