Increased viability of endometrial cells by in vitro treatment with di-(2-ethylhexyl) phthalate

Based on the findings of several reports that have shown an increased plasma level of di-(2-ethylhexyl) phthalate (DEHP) in women with endometriosis, the present study was designed to evaluate whether in vitro treatment with DEHP can increase viability of endometrial cells. Utilizing 3-(4,5-dimethyl...

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Veröffentlicht in:Fertility and sterility 2010-11, Vol.94 (6), p.2413-2416
Hauptverfasser: Kim, Young-Hoon, M.D., Ph.D, Kim, Sung Hoon, M.D., Ph.D, Lee, Hoi Woul, M.S, Chae, Hee Dong, M.D., Ph.D, Kim, Chung-Hoon, M.D., Ph.D, Kang, Byung Moon, M.D., Ph.D
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container_end_page 2416
container_issue 6
container_start_page 2413
container_title Fertility and sterility
container_volume 94
creator Kim, Young-Hoon, M.D., Ph.D
Kim, Sung Hoon, M.D., Ph.D
Lee, Hoi Woul, M.S
Chae, Hee Dong, M.D., Ph.D
Kim, Chung-Hoon, M.D., Ph.D
Kang, Byung Moon, M.D., Ph.D
description Based on the findings of several reports that have shown an increased plasma level of di-(2-ethylhexyl) phthalate (DEHP) in women with endometriosis, the present study was designed to evaluate whether in vitro treatment with DEHP can increase viability of endometrial cells. Utilizing 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide assay, fluorescent activated cell sorter analysis, and microscopic evaluation after Hoechst staining, we revealed that in vitro treatment with DEHP leads to increased viability of Ishikawa cells as well as endometrial stromal cells in serum-free condition and following exposure to hydrogen peroxide, which suggests that exposure to phthalate might play a role in the establishment of endometriosis.
doi_str_mv 10.1016/j.fertnstert.2010.04.027
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subjects Biological and medical sciences
Cell Proliferation - drug effects
Cell Survival - drug effects
Cells, Cultured
Diethylhexyl Phthalate - pharmacology
Dose-Response Relationship, Drug
Drug Evaluation, Preclinical
endocrine disruptor
endometriosis
endometrium
Endometrium - drug effects
Endometrium - physiology
Female
Female genital diseases
Gynecology. Andrology. Obstetrics
Humans
Internal Medicine
Medical sciences
Non tumoral diseases
Obstetrics and Gynecology
pathogenesis
Phthalate
Plasticizers - pharmacology
Stromal Cells - drug effects
Stromal Cells - physiology
Time Factors
Up-Regulation - drug effects
title Increased viability of endometrial cells by in vitro treatment with di-(2-ethylhexyl) phthalate
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