Angiopoietin-1, angiopoietin-2 and Tie-2 receptor expression in human dermal wound repair and scarring
Summary Background The angiopoietin (Ang)/Tie‐2 ligand/receptor system is known to interact with the vascular endothelial growth factor (VEGF) pathway to determine the fate of blood vessels during angiogenesis. However, the precise contribution of this system to angiogenesis and the mechanisms of v...
Gespeichert in:
| Veröffentlicht in: | British journal of dermatology (1951) 2010-11, Vol.163 (5), p.920-927 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 927 |
|---|---|
| container_issue | 5 |
| container_start_page | 920 |
| container_title | British journal of dermatology (1951) |
| container_volume | 163 |
| creator | Staton, C.A. Valluru, M. Hoh, L. Reed, M.W.R. Brown, N.J. |
| description | Summary
Background The angiopoietin (Ang)/Tie‐2 ligand/receptor system is known to interact with the vascular endothelial growth factor (VEGF) pathway to determine the fate of blood vessels during angiogenesis. However, the precise contribution of this system to angiogenesis and the mechanisms of vascular maturation and remodelling in human tissue repair have yet to be elucidated.
Objectives To examine the spatial and temporal expression of Ang‐1, Ang‐2, Tie‐2 and VEGF in relation to angiogenesis in human surgical wounds.
Methods Punch biopsies were taken either from normal unwounded skin (controls) during surgery or from mastectomy scars between 3 days and 2 years postsurgery. Ang‐1, Ang‐2, Tie‐2 and VEGF fibroblast/myofibroblast and endothelial expression were characterized by immunohistochemistry, analysed semiquantitatively and correlated with microvessel density (MVD) and scar age.
Results The expression of VEGF, Ang‐1, Ang‐2 and Tie‐2 in fibroblasts/myofibroblasts was increased significantly in early scars, decreased in older scars and was related to scar age (P |
| doi_str_mv | 10.1111/j.1365-2133.2010.09940.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_761031782</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>761031782</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3870-e916bb7224b36d16d3771d05fa07f92b480d834169345babdfb888e699df71043</originalsourceid><addsrcrecordid>eNpNkU1v1DAQhi0EokvhL6BcEBeyjDOJHR84lEJb0FIuhUq9WE48KV7yhb1Rt_--TndZ8MUznue1ZD-MJRyWPK736yVHUaQZR1xmEE9BqRyW2ydscRg8ZQsAkCkogUfsRQhrAI5QwHN2lIFABFAL1pz0t24YB0cb16f8XWL-77PY2uTKUaw81TRuBp_QdvQUghv6xPXJr6kzfWLJd6ZN7oYp8p5G4_xjNNTGe9ffvmTPGtMGerXfj9mPs89Xpxfp6vv5l9OTVVpjKSElxUVVySzLKxSWC4tScgtFY0A2KqvyEmyJORcK86IylW2qsixJKGUbySHHY_Z2d-_ohz8ThY3uXKipbU1PwxS0FByQyzKL5Os9OVUdWT161xl_r_9-TQTe7AETX9E23vS1C_84RMlRFZH7sOPuXEv3hzkHPavSaz0b0bMRPavSj6r0Vn_8-mmuYj7d5V3Y0PaQN_63FhJloa8vz_XNxeXP1fXNNw34AKGWlPk</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>761031782</pqid></control><display><type>article</type><title>Angiopoietin-1, angiopoietin-2 and Tie-2 receptor expression in human dermal wound repair and scarring</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Staton, C.A. ; Valluru, M. ; Hoh, L. ; Reed, M.W.R. ; Brown, N.J.</creator><creatorcontrib>Staton, C.A. ; Valluru, M. ; Hoh, L. ; Reed, M.W.R. ; Brown, N.J.</creatorcontrib><description>Summary
Background The angiopoietin (Ang)/Tie‐2 ligand/receptor system is known to interact with the vascular endothelial growth factor (VEGF) pathway to determine the fate of blood vessels during angiogenesis. However, the precise contribution of this system to angiogenesis and the mechanisms of vascular maturation and remodelling in human tissue repair have yet to be elucidated.
Objectives To examine the spatial and temporal expression of Ang‐1, Ang‐2, Tie‐2 and VEGF in relation to angiogenesis in human surgical wounds.
Methods Punch biopsies were taken either from normal unwounded skin (controls) during surgery or from mastectomy scars between 3 days and 2 years postsurgery. Ang‐1, Ang‐2, Tie‐2 and VEGF fibroblast/myofibroblast and endothelial expression were characterized by immunohistochemistry, analysed semiquantitatively and correlated with microvessel density (MVD) and scar age.
Results The expression of VEGF, Ang‐1, Ang‐2 and Tie‐2 in fibroblasts/myofibroblasts was increased significantly in early scars, decreased in older scars and was related to scar age (P < 0·001) and MVD (P < 0·0004), with strong correlations between all factors. In contrast, vascular expression of Ang‐1 was decreased slightly in early scars, vascular Ang‐2 remained constant and Tie‐2 vascular expression increased, although there were no correlations with scar age or MVD.
Conclusions These data demonstrate that angiopoietins and their receptor, Tie‐2, are expressed in both fibroblasts/myofibroblasts and endothelial cells in healing human wounds. Fibroblast/myofibroblast expression correlates with angiogenesis and VEGF expression, suggesting a role for the angiopoietin/Tie‐2 system in normal wound repair and scarring.</description><identifier>ISSN: 0007-0963</identifier><identifier>EISSN: 1365-2133</identifier><identifier>DOI: 10.1111/j.1365-2133.2010.09940.x</identifier><identifier>PMID: 20633009</identifier><identifier>CODEN: BJDEAZ</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>angiogenesis ; angiopoietin ; Angiopoietin-1 - metabolism ; Angiopoietin-2 - metabolism ; Biological and medical sciences ; Biopsy ; Cicatrix - metabolism ; Cicatrix - pathology ; Dermatology ; Endothelial Cells - metabolism ; Female ; Fibroblasts - metabolism ; Humans ; Immunohistochemistry ; Medical sciences ; Myofibroblasts - metabolism ; Neovascularization, Physiologic - physiology ; Receptor, TIE-2 - metabolism ; Skin - metabolism ; Skin - pathology ; Tie-2 ; Vascular Endothelial Growth Factor A - metabolism ; VEGF ; wound healing ; Wound Healing - physiology</subject><ispartof>British journal of dermatology (1951), 2010-11, Vol.163 (5), p.920-927</ispartof><rights>2010 The Authors. BJD © 2010 British Association of Dermatologists</rights><rights>2015 INIST-CNRS</rights><rights>2010 The Authors. BJD © 2010 British Association of Dermatologists.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3870-e916bb7224b36d16d3771d05fa07f92b480d834169345babdfb888e699df71043</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2133.2010.09940.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2133.2010.09940.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23371395$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20633009$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Staton, C.A.</creatorcontrib><creatorcontrib>Valluru, M.</creatorcontrib><creatorcontrib>Hoh, L.</creatorcontrib><creatorcontrib>Reed, M.W.R.</creatorcontrib><creatorcontrib>Brown, N.J.</creatorcontrib><title>Angiopoietin-1, angiopoietin-2 and Tie-2 receptor expression in human dermal wound repair and scarring</title><title>British journal of dermatology (1951)</title><addtitle>Br J Dermatol</addtitle><description>Summary
Background The angiopoietin (Ang)/Tie‐2 ligand/receptor system is known to interact with the vascular endothelial growth factor (VEGF) pathway to determine the fate of blood vessels during angiogenesis. However, the precise contribution of this system to angiogenesis and the mechanisms of vascular maturation and remodelling in human tissue repair have yet to be elucidated.
Objectives To examine the spatial and temporal expression of Ang‐1, Ang‐2, Tie‐2 and VEGF in relation to angiogenesis in human surgical wounds.
Methods Punch biopsies were taken either from normal unwounded skin (controls) during surgery or from mastectomy scars between 3 days and 2 years postsurgery. Ang‐1, Ang‐2, Tie‐2 and VEGF fibroblast/myofibroblast and endothelial expression were characterized by immunohistochemistry, analysed semiquantitatively and correlated with microvessel density (MVD) and scar age.
Results The expression of VEGF, Ang‐1, Ang‐2 and Tie‐2 in fibroblasts/myofibroblasts was increased significantly in early scars, decreased in older scars and was related to scar age (P < 0·001) and MVD (P < 0·0004), with strong correlations between all factors. In contrast, vascular expression of Ang‐1 was decreased slightly in early scars, vascular Ang‐2 remained constant and Tie‐2 vascular expression increased, although there were no correlations with scar age or MVD.
Conclusions These data demonstrate that angiopoietins and their receptor, Tie‐2, are expressed in both fibroblasts/myofibroblasts and endothelial cells in healing human wounds. Fibroblast/myofibroblast expression correlates with angiogenesis and VEGF expression, suggesting a role for the angiopoietin/Tie‐2 system in normal wound repair and scarring.</description><subject>angiogenesis</subject><subject>angiopoietin</subject><subject>Angiopoietin-1 - metabolism</subject><subject>Angiopoietin-2 - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biopsy</subject><subject>Cicatrix - metabolism</subject><subject>Cicatrix - pathology</subject><subject>Dermatology</subject><subject>Endothelial Cells - metabolism</subject><subject>Female</subject><subject>Fibroblasts - metabolism</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Medical sciences</subject><subject>Myofibroblasts - metabolism</subject><subject>Neovascularization, Physiologic - physiology</subject><subject>Receptor, TIE-2 - metabolism</subject><subject>Skin - metabolism</subject><subject>Skin - pathology</subject><subject>Tie-2</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>VEGF</subject><subject>wound healing</subject><subject>Wound Healing - physiology</subject><issn>0007-0963</issn><issn>1365-2133</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkU1v1DAQhi0EokvhL6BcEBeyjDOJHR84lEJb0FIuhUq9WE48KV7yhb1Rt_--TndZ8MUznue1ZD-MJRyWPK736yVHUaQZR1xmEE9BqRyW2ydscRg8ZQsAkCkogUfsRQhrAI5QwHN2lIFABFAL1pz0t24YB0cb16f8XWL-77PY2uTKUaw81TRuBp_QdvQUghv6xPXJr6kzfWLJd6ZN7oYp8p5G4_xjNNTGe9ffvmTPGtMGerXfj9mPs89Xpxfp6vv5l9OTVVpjKSElxUVVySzLKxSWC4tScgtFY0A2KqvyEmyJORcK86IylW2qsixJKGUbySHHY_Z2d-_ohz8ThY3uXKipbU1PwxS0FByQyzKL5Os9OVUdWT161xl_r_9-TQTe7AETX9E23vS1C_84RMlRFZH7sOPuXEv3hzkHPavSaz0b0bMRPavSj6r0Vn_8-mmuYj7d5V3Y0PaQN_63FhJloa8vz_XNxeXP1fXNNw34AKGWlPk</recordid><startdate>201011</startdate><enddate>201011</enddate><creator>Staton, C.A.</creator><creator>Valluru, M.</creator><creator>Hoh, L.</creator><creator>Reed, M.W.R.</creator><creator>Brown, N.J.</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201011</creationdate><title>Angiopoietin-1, angiopoietin-2 and Tie-2 receptor expression in human dermal wound repair and scarring</title><author>Staton, C.A. ; Valluru, M. ; Hoh, L. ; Reed, M.W.R. ; Brown, N.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3870-e916bb7224b36d16d3771d05fa07f92b480d834169345babdfb888e699df71043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>angiogenesis</topic><topic>angiopoietin</topic><topic>Angiopoietin-1 - metabolism</topic><topic>Angiopoietin-2 - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biopsy</topic><topic>Cicatrix - metabolism</topic><topic>Cicatrix - pathology</topic><topic>Dermatology</topic><topic>Endothelial Cells - metabolism</topic><topic>Female</topic><topic>Fibroblasts - metabolism</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Medical sciences</topic><topic>Myofibroblasts - metabolism</topic><topic>Neovascularization, Physiologic - physiology</topic><topic>Receptor, TIE-2 - metabolism</topic><topic>Skin - metabolism</topic><topic>Skin - pathology</topic><topic>Tie-2</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>VEGF</topic><topic>wound healing</topic><topic>Wound Healing - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Staton, C.A.</creatorcontrib><creatorcontrib>Valluru, M.</creatorcontrib><creatorcontrib>Hoh, L.</creatorcontrib><creatorcontrib>Reed, M.W.R.</creatorcontrib><creatorcontrib>Brown, N.J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of dermatology (1951)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Staton, C.A.</au><au>Valluru, M.</au><au>Hoh, L.</au><au>Reed, M.W.R.</au><au>Brown, N.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Angiopoietin-1, angiopoietin-2 and Tie-2 receptor expression in human dermal wound repair and scarring</atitle><jtitle>British journal of dermatology (1951)</jtitle><addtitle>Br J Dermatol</addtitle><date>2010-11</date><risdate>2010</risdate><volume>163</volume><issue>5</issue><spage>920</spage><epage>927</epage><pages>920-927</pages><issn>0007-0963</issn><eissn>1365-2133</eissn><coden>BJDEAZ</coden><abstract>Summary
Background The angiopoietin (Ang)/Tie‐2 ligand/receptor system is known to interact with the vascular endothelial growth factor (VEGF) pathway to determine the fate of blood vessels during angiogenesis. However, the precise contribution of this system to angiogenesis and the mechanisms of vascular maturation and remodelling in human tissue repair have yet to be elucidated.
Objectives To examine the spatial and temporal expression of Ang‐1, Ang‐2, Tie‐2 and VEGF in relation to angiogenesis in human surgical wounds.
Methods Punch biopsies were taken either from normal unwounded skin (controls) during surgery or from mastectomy scars between 3 days and 2 years postsurgery. Ang‐1, Ang‐2, Tie‐2 and VEGF fibroblast/myofibroblast and endothelial expression were characterized by immunohistochemistry, analysed semiquantitatively and correlated with microvessel density (MVD) and scar age.
Results The expression of VEGF, Ang‐1, Ang‐2 and Tie‐2 in fibroblasts/myofibroblasts was increased significantly in early scars, decreased in older scars and was related to scar age (P < 0·001) and MVD (P < 0·0004), with strong correlations between all factors. In contrast, vascular expression of Ang‐1 was decreased slightly in early scars, vascular Ang‐2 remained constant and Tie‐2 vascular expression increased, although there were no correlations with scar age or MVD.
Conclusions These data demonstrate that angiopoietins and their receptor, Tie‐2, are expressed in both fibroblasts/myofibroblasts and endothelial cells in healing human wounds. Fibroblast/myofibroblast expression correlates with angiogenesis and VEGF expression, suggesting a role for the angiopoietin/Tie‐2 system in normal wound repair and scarring.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>20633009</pmid><doi>10.1111/j.1365-2133.2010.09940.x</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0007-0963 |
| ispartof | British journal of dermatology (1951), 2010-11, Vol.163 (5), p.920-927 |
| issn | 0007-0963 1365-2133 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_761031782 |
| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | angiogenesis angiopoietin Angiopoietin-1 - metabolism Angiopoietin-2 - metabolism Biological and medical sciences Biopsy Cicatrix - metabolism Cicatrix - pathology Dermatology Endothelial Cells - metabolism Female Fibroblasts - metabolism Humans Immunohistochemistry Medical sciences Myofibroblasts - metabolism Neovascularization, Physiologic - physiology Receptor, TIE-2 - metabolism Skin - metabolism Skin - pathology Tie-2 Vascular Endothelial Growth Factor A - metabolism VEGF wound healing Wound Healing - physiology |
| title | Angiopoietin-1, angiopoietin-2 and Tie-2 receptor expression in human dermal wound repair and scarring |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T20%3A14%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Angiopoietin-1,%20angiopoietin-2%20and%20Tie-2%20receptor%20expression%20in%20human%20dermal%20wound%20repair%20and%20scarring&rft.jtitle=British%20journal%20of%20dermatology%20(1951)&rft.au=Staton,%20C.A.&rft.date=2010-11&rft.volume=163&rft.issue=5&rft.spage=920&rft.epage=927&rft.pages=920-927&rft.issn=0007-0963&rft.eissn=1365-2133&rft.coden=BJDEAZ&rft_id=info:doi/10.1111/j.1365-2133.2010.09940.x&rft_dat=%3Cproquest_pubme%3E761031782%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=761031782&rft_id=info:pmid/20633009&rfr_iscdi=true |